scholarly journals Lamellar keratoplasty in rabbits using an allogeneic free omental graft and omentum-derived mesenchymal cells associated with the canine amniotic membrane

2016 ◽  
Vol 46 (10) ◽  
pp. 1838-1845 ◽  
Author(s):  
Séfora Vieira da Silva Gouvêa de Barros ◽  
Marcela Aldrovani ◽  
Luciana Cenço Correa de Lacerda ◽  
Mônica Horr ◽  
Fábio Andrade Marinho ◽  
...  
Keyword(s):  
Amniotic Membrane ◽  
Clinical Signs ◽  
Corneal Opacity ◽  
Mesenchymal Cells ◽  
Lamellar Keratoplasty ◽  
Cell Group ◽  
Ki 67 ◽  
Clinical Assessments ◽  
Histopathological Evaluation ◽  
Omental Graft

ABSTRACT: The objective of this research was to evaluate the clinical and microscopic effects in rabbits of lamellar keratoplasty using allogeneic omentum associated with canine amniotic membrane (AM). Rabbits were divided into two groups: one received the allogeneic free omental graft covered with the AM (OM-graft group), while the other received the AM graft containing omental mesenchymal cells (OM-cell group). Clinical signs were evaluated on different postoperative days. After the clinical assessments, the rabbits were euthanized and their corneas were obtained for histopathology and immunohistochemistry (Ki-67, marker for proliferation). Both groups showed chemosis, blepharospasm, eye discharge, hyperemia, and corneal opacity/edema. Neovascularization was observed in the OM-cell group. Histopathological evaluation revealed epithelial islands within the stroma of OM-cell samples. Thirty days after surgery, complete corneal re-epithelialization had occurred in both groups. The OM-cell group showed more Ki-67 positive cells. The free omentum and its cells, combined with the AM, contributed to corneal repair, a process that was completed 30 days after lamellar keratoplasty.

Bioresorbable electrospun nanofibrous scaffolds loaded with bioactive molecules

e-Polymers ◽  
2009 ◽  
Vol 9 (1) ◽  
Author(s):  
Chiara Gualandi ◽  
Piotr Wilczek ◽  
Maria Letizia Focarete ◽  
Gianandrea Pasquinelli ◽  
Michal Kawalec ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Degradation Kinetics ◽  
Hydrolytic Degradation ◽  
Mesenchymal Cells ◽  
Bioactive Molecules ◽  
Differential Interference Contrast Microscopy ◽  
Electrospinning Technique ◽  
Ki 67 ◽  
Nanofibrous Scaffolds

AbstractElectrospinning technology is used to fabricate sub-micrometric fiber mats made of a random equimolar poly(lactide-co-glycolide) copolymer (PLGA), whose in vitro hydrolytic degradation kinetics is investigated over a period of 49 days in phosphate buffer at 37 °C. The PLGA mats show a decrease of molecular weight (by GPC) from the very beginning of the experiment, whereas a macroscopic weight loss from the samples is appreciated (by gravimetry) only after 20 days of buffer exposure. The molar mass distribution curves remain monomodal during the degradation experiment suggesting that no acid auto-catalyzed hydrolysis, commonly observed in bulk specimens, occurs in sub-micrometric PLGA fibers. PLGA scaffolds containing Endothelial Growth Factor Supplement (ECGS) were also fabricated by electrospinning, from ECGS-containing polymer solutions. Mesenchymal cells derived from human bone marrow mononuclear cells were cultured in the presence of such ECGS-loaded PLGA scaffolds. Flow cytometry and Differential Interference Contrast microscopy were used to characterize the cell cultures over a 7 day period. The results of AnexinV/PI staining and of intranuclear Ki-67 protein expression show, together with concomitant cell morphology modifications, that growth factors released from the scaffolds support the survival, proliferation and growth of the mesenchymal cells. This result demonstrates that ECGS maintains its bioactivity upon release from the electrospun fibers and shows the versatility of the electrospinning technique.

Molybdenosis Leading to Type 2 Diabetes Mellitus in Swedish Moose

2003 ◽  
Author(s):  
Adrian Frank
Keyword(s):  
Trace Element ◽  
Experimental Studies ◽  
Clinical Signs ◽  
Corneal Opacity ◽  
Chemical Parameters ◽  
Trace Element Pattern ◽  
Cu Deficiency ◽  
Mucosal Oedema ◽  
Loss Of Appetite ◽  
Physical And Chemical

The “mysterious moose disease” also called “wasting disease” is affecting moose in a strongly acidified region of southwestern Sweden. Chemical investigations of animals from the affected region have been performed since 1988 and several articles are already published (Frank et al. 1994, Frank 1998, Frank et al. 1999, 2000a, b, c, d). The numerous clinical signs and necropsy findings have included diarrhea, loss of appetite, emaciation, discoloration and loss of hair, apathy, osteoporosis, and neurological signs such as behavioral and locomotor disturbances (Rehbinder et al. 1991, Stéen et al. 1993). Further findings were mucosal oedema, hyperemia, hemorrhages and lesions of the mucosa in the gastrointestinal tract, hemosiderosis of the spleen and liver, dilated flabby heart, alveolar emphysema, and uni- or bilateral corneal opacity. Not all the symptoms appear simultaneously in one and the same animal. About 150—180 affected animals have been reported annually since the late 1980s. An increase in molybdenum (Mo) and a decrease in copper and cadmium (Cu, Cd) content in organ tissues (e.g., liver) are signs of a disturbed trace element balance found in affected animals (Frank 1998). To confirm the findings and to elucidate the mechanisms leading to molybdenosis and Cu deficiency, experimental studies were performed in goats. The feeding studies were performed in a controlled laboratory environment and a semi-synthetic diet was supplied (Frank et al. 2000c). Despite considerable differences in species and living conditions between goat and moose, similar changes in trace element pattern and clinical chemical parameters were observed in both species. The study shows that the etiology of the moose disease is basically molybdenosis followed by Cu deficiency, inter alia (Frank et al. 2000a,b,d). Mo is an essential trace element that controls the metabolism of Cu in ruminants. Increased Mo concentrations relative to Cu in feed results in Cu deficiency, whereas the converse leads to an accumulation of Cu, even to Cu poisoning (e.g., in sheep). In an acidified environment, the molybdate anion is adsorbed in the soil, contrary to positively charged metals. The presence of Mo and Cu in the environment is basically dependent mainly on geochemistry, influenced by numerous physical and chemical parameters (Selinus et al. 1996, Selinus and Frank 2000).

scholarly journals Management of dislocated nucleus with corneal opacity by combined deep anterior lamellar keratoplasty, pars- plana vitrectomy and phaco-fragmentation

2019 ◽  
Vol 12 (6) ◽  
pp. e229397
Author(s):  
Samendra Karkhur ◽  
Alok Sen ◽  
Gautam Parmar ◽  
Priyavrat Bhatia
Keyword(s):  
Ciliary Body ◽  
Surgical Intervention ◽  
Corneal Opacity ◽  
Ultrasound Biomicroscopy ◽  
Lamellar Keratoplasty ◽  
Deep Anterior Lamellar Keratoplasty ◽  
Anterior Lamellar Keratoplasty ◽  
Ciliary Processes ◽  
Pars Plana ◽  
The Right

A 32-year-old man presented with bilateral diminution of vision for 10 years. Visual acuity was light perception in both eyes with inaccurate projection in the oculus dextrus(OD) and accurate projection in the in oculus sinister (OS). Intraocular pressure was 6 and 12 mm Hg in the OD and OS, respectively. Slit-lamp examination revealed the presence of leucomatous corneal opacification with microcornea in oculus uterque (OU). Both eyes had a dislocated nucleus in the vitreous cavity on ultrasound B-scan with advanced cupping in the right eye. Ultrasound biomicroscopy revealed ciliary body atrophy in the OD and preserved ciliary processes (two quadrants) in the OS. In view of a partially preserved ciliary body function and a potential for gaining useful vision, we planned surgical intervention in the left eye. Deep anterior lamellar keratoplasty with pars planavitrectomy (PPV) and phacofragmentation of the nucleus was done. Donor lamellar graft was sutured after completion of PPV and nucleus fragmentation. The patient regained useful ambulatory vision postoperatively with clear corneal graft.

scholarly journals Both Freshly Prepared and Frozen-Stored Amniotic Membrane Cells Express the Complement Inhibitor CD59

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Ágnes Füst ◽  
Éva Pállinger ◽  
Adrienn Stündl ◽  
Eszter Kovács ◽  
László Imre ◽  
...  
Keyword(s):  
Cell Surface ◽  
Amniotic Membrane ◽  
Ocular Surface ◽  
Cell Types ◽  
Surface Expression ◽  
Mesenchymal Cells ◽  
Cell Surface Expression ◽  
Complement Inhibitor ◽  
Becton Dickinson ◽  
Intracellular Expression

Amniotic membrane proved to be very effective tool in the treatment of a number of ocular surface diseases. The amniotic membrane, however, has to be stored before its transplantation onto the ocular surface followed by mandatory serologic control in order to exclude the transmission of certain viruses. Therefore it is most important to study if cryopreservation of the membrane affects cell surface expression of the molecules. We measured cell surface expression of CD59, a membrane-bound complement inhibitor on the cells of freshly prepared and cryopreserved amniotic membrane. Cells of amniotic membrane were separated mechanically. Epithelial and mesenchymal cells were identified by the intracellular expression of nanog and the cell surface ICAM1 positivity, respectively. Multicolor flow cytometric immunophenotyping was used for determination of the CD59 expression. CellQuest-Pro software program (Becton Dickinson) was used both for measurements and analysis. CD59-positive cells could be detected in all investigated samples and in all investigated cell types, although the expression level of CD59 differed. CD59 was expressed both on freshly prepared and frozen-stored samples. Higher level of CD59 was detected on ICAM1+ mesenchymal cells than on nanog+ epithelial cells. Our findings indicate that amniotic membranes maintain their complement inhibiting capacity after cryopreservation.

scholarly journals Amniotic Membrane-Derived Mesenchymal Cells and Their Conditioned Media: Potential Candidates for Uterine Regenerative Therapy in the Horse

PLoS ONE ◽  
2014 ◽  
Vol 9 (10) ◽  
pp. e111324 ◽  
Author(s):  
Bruna Corradetti ◽  
Alessio Correani ◽  
Alessio Romaldini ◽  
Maria Giovanna Marini ◽  
Davide Bizzaro ◽  
...  
Keyword(s):  
Amniotic Membrane ◽  
Mesenchymal Cells ◽  
Conditioned Media ◽  
Regenerative Therapy

scholarly journals Silent somatotroph tumour revisited from a study of 80 patients with and without acromegaly and a review of the literature

2017 ◽  
Vol 176 (2) ◽  
pp. 195-201 ◽  
Author(s):  
Laura Chinezu ◽  
Alexandre Vasiljevic ◽  
Jacqueline Trouillas ◽  
Marion Lapoirie ◽  
Emmanuel Jouanneau ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Transcription Factor ◽  
Somatostatin Receptors ◽  
Clinical Signs ◽  
Secretory Activity ◽  
Thyroid Stimulating Hormone ◽  
Lower Percentage ◽  
Review Of The Literature ◽  
Pituitary Tumours ◽  
Ki 67

Background Silent somatotroph tumours are growth hormone (GH) immunoreactive (IR) pituitary tumours without clinical and biological signs of acromegaly. Their better characterisation is required to improve the diagnosis. Materials and methods Twenty-one silent somatotroph tumours were compared to 59 somatotroph tumours with acromegaly. Tumours in each group were classified into GH and plurihormonal (GH/prolactin (PRL)/±thyroid-stimulating hormone (TSH)) and into densely granulated (DG) and sparsely granulated (SG) types. The two groups were then compared with regards to proliferation (Ki-67, p53 indexes and mitotic count), differentiation (expression of somatostatin receptors SSTR2A–SSTR5 and transcription factor Pit-1) and secretory activity (% of GH- and PRL-IR cells). Results The silent somatotroph tumours represented 2% of all tested pituitary tumours combined. They were more frequent in women than in men (P = 0.002), more frequently plurihormonal and SG (P < 0.01), with a lower percentage of GH-IR cells (P < 0.0001) compared to those with acromegaly. They all expressed SSTR2A, SSTR5 and Pit-1. The plurihormonal (GH/PRL/±TSH) tumours were mostly observed in women (sex ratio: 3/1) and in patients who were generally younger than those with acromegaly (P < 0.001). They were larger (P < 0.001) with a higher Ki-67 index (P = 0.007). Conclusions The silent somatotroph tumours are not uncommon. Their pathological diagnosis requires the immunodetection of GH and Pit-1. They are more frequently plurihormonal and more proliferative than those with acromegaly. A low secretory activity of these tumours might explain the normal plasma values for GH and insulin-like growth factor 1 (IGF1) and the absence of clinical signs of acromegaly.

Lamellar keratoplasty in rabbits using human and rabbit amniotic membrane grafts: a comparative study

2013 ◽  
Vol 18 (3) ◽  
pp. 191-197 ◽  
Author(s):  
Cintia A. L. Godoy-Esteves ◽  
José Álvaro P. Gomes ◽  
Karina Yazbek ◽  
José L. Guerra ◽  
Paulo S. M. Barros
Keyword(s):  
Comparative Study ◽  
Amniotic Membrane ◽  
Lamellar Keratoplasty

scholarly journals Tectonic DSAEK for the Management of Impending Corneal Perforation

2012 ◽  
Vol 2012 ◽  
pp. 1-4
Author(s):  
Enrique O. Graue-Hernandez ◽  
Isaac Zuñiga-Gonzalez ◽  
Julio C. Hernandez-Camarena ◽  
Martha Jaimes ◽  
Patricia Chirinos-Saldaña ◽  
...  
Keyword(s):  
Dry Eye ◽  
Amniotic Membrane ◽  
Ocular Surface ◽  
Autologous Serum ◽  
Lamellar Keratoplasty ◽  
Corneal Perforation ◽  
Structural Support ◽  
History Of ◽  
The Right ◽  
Corneal Thinning

Purpose. To report a case of severe corneal thinning secondary to dry eye treated with a tectonic Descemet stripping automated lamellar keratoplasty (DSAEK) and amniotic membrane graft.Methods. A 72-year-old man with a history of long standing diabetes mellitus type 2 and dry eye presented with 80% corneal thinning and edema on the right eye and no signs of infectious disease, initially managed with topical unpreserved lubrication and 20% autologous serum drops. Eight weeks after, the defect advanced in size and depth until Descemetocele was formed. Thereafter, he underwent DSAEK for tectonic purposes. One month after the procedure, the posterior lamellar graft was well adhered but a 4 mm epithelial defect was still present. A multilayered amniotic membrane graft was then performed.Results. Ocular surface healed quickly and reepithelization occurred over a 2-week period. Eight months after, the ocular surface remained stable and structurally adequate.Conclusion. Tectonic DSAEK in conjunction with multilayered amniotic graft may not only provide structural support and avoid corneal perforation, but may also promote reepithelization and ocular surface healing and decrease concomitant inflammation.

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