scholarly journals Improvement of the viability of probiotics (Lactobacillus acidophilus) by multilayer encapsulation

2019 ◽  
Vol 49 (9) ◽  
Author(s):  
Maria Fernanda da Silveira Cáceres de Menezes ◽  
Thaiane Marques da Silva ◽  
Mariana de Araújo Etchepare ◽  
Bruna de Souza Fonseca ◽  
Vandré Pinto Sonza ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Gastrointestinal Tract ◽  
Whey Protein ◽  
Lactobacillus Acidophilus ◽  
Gastrointestinal System ◽  
Ionic Gelation ◽  
Gastrointestinal Conditions ◽  
In Vitro Exposure ◽  
Free Cells

ABSTRACT: This study produced pectin microcapsules containing Lactobacillus acidophilus by external ionic gelation, followed by the adsorption of whey protein and pectin to form multilayers. The viability of free and microencapsulated lactobacilli was evaluated after in vitro exposure to gastrointestinal conditions. They were also assessed by heat treatment, and stability was examined at -18 °C, 5 °C and 25 °C for 120 days. Exposure to different pHs, simulating passage through the gastrointestinal tract, showed that treatment of the microcapsules with only pectin (LA/P0) and with one and two layers of whey protein (treatments LA/P1 and LA/P3, respectively), were able to protect Lactobacillus acidophilus , with microcapsules increasing the release of probiotics from the stomach into the intestines. Free cells showed a decrease in their counts over the course of the simulated gastrointestinal system. Regarding heat treatments, microcapsules with a layer of whey protein (LA/P1) maintained the viability of their encapsulated Lactobacillus acidophilus (9.57 log CFU/g-1). The best storage viability was at -18 °C, with a count of 7.86 log CFU/g-1at 120 days for microcapsule LA/P1,with those consisting of two layers of whey protein (LA/P3)having a 6.55 log CFU/g-1 at 105 days. This study indicated that external ionic gelation was effective and could be used for the production of pectin microcapsules, with multilayer whey protein promoting greater protection and viability of Lactobacillus acidophilus.

scholarly journals Viability of Lactobacillus acidophilus La5 in pectin–whey protein microparticles during exposure to simulated gastrointestinal conditions

2013 ◽  
Vol 51 (2) ◽  
pp. 872-878 ◽  
Author(s):  
Clarice Gebara ◽  
Karina S. Chaves ◽  
Maria Cecília E. Ribeiro ◽  
Flavia N. Souza ◽  
Carlos R.F. Grosso ◽  
...  
Keyword(s):  
Whey Protein ◽  
Lactobacillus Acidophilus ◽  
Gastrointestinal Conditions ◽  
Lactobacillus Acidophilus La5 ◽  
Protein Microparticles

scholarly journals Modification of protein structures by altering the whey protein profile and heat treatment affects in vitro static digestion of model infant milk formulas

2020 ◽  
Vol 11 (8) ◽  
pp. 6933-6945
Author(s):  
Amira Halabi ◽  
Thomas Croguennec ◽  
Said Bouhallab ◽  
Didier Dupont ◽  
Amélie Deglaire
Keyword(s):  
Heat Treatment ◽  
Whey Protein ◽  
Protein Profile ◽  
Protein Structures

The aim was to investigate how protein structures in model infant milk formulas affect proteolysis kinetics during in vitro digestion.

Dynamic gastric stability and in vitro lipid digestion of whey-protein-stabilised emulsions: Effect of heat treatment

2020 ◽  
Vol 318 ◽  
pp. 126463 ◽  
Author(s):  
Aiqian Ye ◽  
Xin Wang ◽  
Quanquan Lin ◽  
Jianzhong Han ◽  
Harjinder Singh
Keyword(s):  
Heat Treatment ◽  
Whey Protein ◽  
Lipid Digestion

scholarly journals Effect of Simulated Gastrointestinal Conditions on Biofilm Formation bySalmonella1,4,[5],12:i:-

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
R. Seixas ◽  
M. Gabriel ◽  
J. Machado ◽  
L. Tavares ◽  
F. Bernardo ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Biofilm Formation ◽  
Contributing Factors ◽  
Biofilm Growth ◽  
Biofilm Production ◽  
Gastrointestinal Conditions ◽  
Mueller Hinton ◽  
Mueller Hinton Broth

SalmonellaTyphimurium 1,4,[5],12:i:- is a major serovar responsible for human salmonellosis whose biofilm-forming ability, influenced by environmental conditions like those found in the gastrointestinal tract, is one of the main contributing factors to its ability to persist in the host and thus one of the main causes of chronic relapsing infections. Most studies to evaluate biofilm formation are performed in microtiter assays using standard media. However, no reports are available on the ability of this serovar to produce biofilm underin vitrosimulated gastrointestinal conditions which better correlate with the environment found in the gastrointestinal tract. To address this, a modified biofilm assay simulating intestinal fluid was conceived to assess the biofilm formation of 133SalmonellaTyphimurium 1,4,[5],12:i:- isolates with and without agitation and at three different time points (24 h, 48 h, and 72 h). The results were then compared to the existing microtiter method using conventional biofilm growth medium (Mueller Hinton Broth). Statistical analysis revealed significant differences in the results obtained between the three protocols used. The simulated human intestinal environment impaired biofilm production demonstrating that conditions like pH, agitation or the presence of enzymes can influence biofilm production. Therefore, results fromin vitrosimulation ofin vivoconditions may contribute to unravelling factors relating to biofilm formation and persistence in the context of the human host.

A prebiotic mixture improved Lactobacillus acidophilus and Bifidobacterium animalis gastrointestinal in vitro resistance in petit-suisse

2016 ◽  
Vol 7 (5) ◽  
pp. 2312-2319 ◽  
Author(s):  
Marina Padilha ◽  
Martha Lissete Villarreal Morales ◽  
Antonio Diogo Silva Vieira ◽  
Mayra Garcia Maia Costa ◽  
Susana Marta Isay Saad
Keyword(s):  
Lactobacillus Acidophilus ◽  
Bifidobacterium Animalis ◽  
Gastrointestinal Conditions ◽  
Probiotic Strains

Advantageous addition of a prebiotic mixture in petit-suisse cheese, improved the probiotic strains survival under gastrointestinal conditions simulated in vitro throughout 28 days of storage.

Encapsulation of Lactobacillus acidophilus NCDC 016 cells by spray drying: characterization, survival after in vitro digestion, and storage stability

2020 ◽  
Vol 11 (10) ◽  
pp. 8694-8706
Author(s):  
Divyasree Arepally ◽  
Ravula Sudharshan Reddy ◽  
Tridib Kumar Goswami
Keyword(s):  
Spray Drying ◽  
Lactobacillus Acidophilus ◽  
Storage Stability ◽  
Gum Arabic ◽  
In Vitro Digestion ◽  
Encapsulated Cells ◽  
Gastrointestinal Conditions ◽  
Drying Technology ◽  
And Storage

L. acidophilus was encapsulated with maltodextrin and different concentrations of gum arabic by spray drying technology. Encapsulated cells have shown better viability under simulated gastrointestinal conditions compared to free cells.

Sign in / Sign up

Export Citation Format

Share Document