scholarly journals EROSIVE POTENTIAL OF SPORTS BEVERAGES ON HUMAN ENAMEL “IN VITRO”

2018 ◽  
Vol 24 (5) ◽  
pp. 386-390
Author(s):  
Dalila Meazza Damo ◽  
Guilherme Anziliero Arossi ◽  
Helena Alvez da Silva ◽  
Leonardo Haerter dos Santos ◽  
Diego Rafael Kappaun
Keyword(s):  
Vickers Microhardness ◽  
Tooth Enamel ◽  
Low Ph ◽  
Control Surface ◽  
Test Surface ◽  
Results Of Treatment ◽  
Sports Drinks ◽  
Human Enamel ◽  
The Mean

ABSTRACT Introduction: The low pH of sports drinks may cause tooth enamel demineralization. Objective: To measure Vickers hardness of human enamel exposed to sports drinks. Methods: Human molars were used to collect the enamel samples. Each sample had a test surface (exposed to the drinks) and a control surface (unexposed). The samples were exposed to isotonic drinks Gatorade and Powerade, and to maltodextrin drinks Malto Advanced and Malto Active, for 10 minutes every 12 hours over 30 days. The Vickers microhardness test was conducted with three indentations on each surface. The mean of the indentations within each group was considered in the statistical analysis. Sports drinks variables were analyzed with ANOVA/Tukey (p≤0.01). The independent t-test was used in the comparison between the control and test surfaces of each drink (p ≤ 0.05). Results: Enamel exposure to Gatorade (p = 0.000) Malto Advanced (p = 0.000) and Malto Active (p = 0.000) was seen to significantly reduce microhardness, while the isotonic drink Powerade had no significant effect on enamel (p = 0.248). Conclusion: It was concluded that with the exception of the isotonic drink Powerade, all the sports drinks tested caused a reduction in the microhardness of human enamel. Evidence Level III; Therapeutic studies - Investigating the Results of Treatment.

scholarly journals Remineralizing Effect of Topical NovaMin and Nanohydroxyapatite on Caries-like Lesions in Primary Teeth

2016 ◽  
Vol 17 (8) ◽  
pp. 645-649 ◽  
Author(s):  
Roza Haghgoo ◽  
Motahare Ahmadvand ◽  
Sareh Moshaverinia
Keyword(s):  
Primary Teeth ◽  
Vickers Microhardness ◽  
Tooth Enamel ◽  
Dental Enamel ◽  
Carbonate Apatite ◽  
Anterior Teeth ◽  
Significant Difference ◽  
The Mean ◽  
Caries Lesions

ABSTRACT Introduction NovaMin is a synthetic mineral compound composed of calcium, sodium, phosphorus, and silica. It releases crystalline hydroxyl-carbonate apatite (HCA), which structurally resembles the minerals naturally found in the teeth. Nanohydroxyapatite (NHA) is a biocompatible compound with high affinity for tooth enamel. NHA particles morphologically resemble dental enamel apatite crystals. Considering the efficacy of remineralizing agents and the importance of conservative preventive measures, this study aimed to compare the remineralizing effects of NovaMin and NHA on caries-like lesions in primary teeth. Materials and methods This in vitro experimental study was conducted on 30 sound human primary anterior teeth with no cracks or fractures. The surface microhardness (SMH) of each tooth was measured at baseline using a Vickers microhardness tester. The teeth were then subjected to remineralization/ demineralization cycles, and artificial caries lesions were created in them. The SMH of each tooth was measured again and the teeth were then randomly treated with toothpastes containing NovaMin or 10% NHA powder for 2 minutes daily for a period of 5 days. The SMH of each was again measured afterward. Data were statistically analyzed using independent t-tests and Mann–Whitney U tests. Results The mean SMH was found to be higher in the teeth treated with NovaMin toothpaste (422.67 kgf/mm2) than in the teeth treated with NHA (384.2 kgf/mm2); However, this difference was not statistically significant. Conclusion Both NHA and NovaMin were effective for remineralization of caries-like lesions of primary teeth and no significant difference was detected in their efficacy. How to cite this article Haghgoo R, Ahmadvand M, Moshaverinia S. Remineralizing Effect of Topical NovaMin and Nano-hydroxyapatite on Caries-like Lesions in Primary Teeth. J Contemp Dent Pract 2016;17(8):645-649.

scholarly journals Analysis of the surface of healthy and fluorotic human enamel using microhardness test

2017 ◽  
Vol 30 (1) ◽  
pp. 6-9
Author(s):  
Ana Josefina Monjarás Ávila ◽  
Veronica Zavala Alonso ◽  
Grace Morales Alcocer ◽  
Gabriel Alejandro Martínez Castañón ◽  
Nuria Patiño Marín ◽  
...  
Keyword(s):  
Tooth Enamel ◽  
Dental Fluorosis ◽  
Essential Property ◽  
Surface Microhardness ◽  
Average Standard Deviation ◽  
Mean Values ◽  
Hardness Tester ◽  
Tukey Test ◽  
Human Enamel ◽  
The Mean

The microhardness is an essential property of tooth enamel; there may be many factors that alter or diminish this quality causing weakness, one of which is dental fluorosis. The aim of this study was evaluate the surface microhardness of fluorotic enamel compared with healthy enamel. Two hundred forty extracted human molars were classified into four groups: Healthy (H), mild (MI), moderate (MO) and severe (S) fluorosis according to the Dean index. All samples were analyzed by Micro Vickers Hardness Tester. Average, standard deviation and ranges were calculated for quantitative variables, the ANOVA and Tukey test was used to identify differences between groups. The mean values of surface microhardness in HVN were: H, 333.4; MI, 290.3; MO, 266.1; S, 252.0. The differences between mean surface microhardness among healthy group and fluorotic groups were statistically significant (p

scholarly journals A Comparative Study of Different Bleaching Agents on the Morphology of Human Enamel: An in vitro SEM Study

2012 ◽  
Vol 13 (6) ◽  
pp. 756-759 ◽  
Author(s):  
Roshan Uthappa ◽  
ML Suprith ◽  
Shreetha Bhandary ◽  
Sumit Dash
Keyword(s):  
Phosphoric Acid ◽  
Comparative Study ◽  
Tooth Enamel ◽  
Extended Period ◽  
Enamel Surface ◽  
Control Group ◽  
Human Enamel ◽  
Sem Study ◽  
Labial Surface

ABSTRACT The purpose of the study was to compare two different commercial bleaching agents, Opalescence with Colgate Platinum, and 30% phosphoric acid used as aggressive agent on the morphology of human enamel. Materials and methods Ten freshly extracted, noncarious, human maxillary central incisors extracted for periodontal reasons were used in this study. The labial surface of the disinfected teeth were polished using a polishing paste with the help of rubber cup and a slow speed handpiece. Each tooth was sectioned at cement-enamel junction and the crown was separated into four specimens, all taken from labial surface. Group 1 was treated with Colgate Platinum for 7 hours, group 2 with Opalescence for 7 hours, group 3 was treated with 30% phosphoric acid for 30 seconds and group 4 was untreated and used as control. After the treatment period, the specimens were washed with normal saline and stored in sterile bottle and sealed. Photomicrographs obtained from the scanning electron microscopy (SEM) after surface treatments were examined for no alteration, slight alteration, moderate alteration and severe alterations. Results The specimens treated with commercial bleaching agents revealed no enamel surface morphologic alterations compared to control group. The specimen treated with phosphoric acid showed severe alterations. Conclusion Ten percent carbamide peroxide evaluated in this study does not etch tooth enamel or alter enamel surface morphology as do conventional etching techniques. Clinical relevance Carbamine peroxide is a safe and effective tooth whitening agent even when used for extended period of time. The enamel surface remains smooth which reduces caries due to plaque collection. How to cite this article Uthappa R, Suprith ML, Bhandary S, Dash S. A Comparative Study of Different Bleaching Agents on the Morphology of Human Enamel: An in vitro SEM Study. J Contemp Dent Pract 2012;13(6):756-759.

scholarly journals Effects of Two In-Office Bleaching Agents with Different pH on the Structure of Human Enamel: An In Situ and In Vitro Study

2013 ◽  
Vol 38 (1) ◽  
pp. 100-110 ◽  
Author(s):  
Y Sa ◽  
L Sun ◽  
Z Wang ◽  
X Ma ◽  
S Liang ◽  
...  
Keyword(s):  
Artificial Saliva ◽  
In Vitro Study ◽  
Storage Condition ◽  
Human Saliva ◽  
Low Ph ◽  
Force Microscopy ◽  
Human Enamel ◽  
And Control

SUMMARY This study evaluated the effects of two in-office bleaching agents (Beyond and Opalescence Boost) with different pH on the structure and mechanical properties of human enamel in vitro and in situ. One hundred and eight enamel slabs were obtained from freshly extracted premolars. The specimens were randomly distributed into nine groups (n=12), and the human saliva (HS) in the volunteers' oral cavities was used to simulate the in situ condition: Beyond + HS, Opalescence Boost (O-Boost) + HS, Control + HS, Beyond + artificial saliva (AS), O-Boost + AS, Control + AS, Beyond + distilled water (DW), O-Boost + DW, and Control + DW. The bleaching treatments were performed on the first and eighth day, and the total bleaching time was 90 minutes. Baseline and final surface roughness (RMS), surface morphology, microhardness, and fracture toughness (FT) were measured before the treatment and on the fifteenth day, respectively. Compared with control groups, surface alterations on enamel were found in the Beyond + AS and Beyond + DW groups under atomic force microscopy evaluation. Two-way analysis of variance and Tukey test revealed that the RMS showed significant intergroup differences for both storage condition and bleaching agent, whereas microhardness and FT revealed no significant alteration. The results indicated that in-office bleaching agents with low pH values could induce enamel morphology alterations under in vitro conditions. The presence of natural HS could eliminate the demineralization effect caused by low pH.

scholarly journals In vitro effect of energy drinks on human enamel surface

2018 ◽  
Vol 47 (1) ◽  
pp. 57-62
Author(s):  
Marise Sano Suga MATUMOTO ◽  
Raquel Sano Suga TERADA ◽  
Dayla Thyeme HIGASHI ◽  
Mitsue FUJIMAKI ◽  
Selma Sano SUGA ◽  
...  
Keyword(s):  
Titratable Acidity ◽  
Energy Drinks ◽  
Low Ph ◽  
Enamel Surface ◽  
Human Enamel ◽  
Knoop Microhardness ◽  
Vitro Effect ◽  
Red Bull ◽  
Acid Challenge

Abstract Introduction Energy drinks (ED) possess low pH and citric acid in their composition, making them potentially erosive beverages that can contribute to the high dental erosion rates found currently in the general population and also in young people. Objective To evaluate the mean pH and titratable acidity of commercial ED and the influence of a brand of ED on the superficial microhardness of human enamel. Material and method Ten commercial ED were selected and the pH of two lots of each ED with and without gas was obtained. Acid titration was conducted with the addition of NaOH aliquots until the pH 7 was reached. Eighteen human enamel specimens were allocated in three groups (N=6), Red Bull (RB), Red Bull Light (RBL) and distilled water (C), submitted to an acid challenge with the ED, six consecutive times, with 12 hours intervals, during three days. Knoop microhardness was measured before and after the acid challenge. Result All ED brands tested presented low pH levels ranging from 2.1 to 3.2. Regarding titratable acidity, it was found that the amount of base required promoting the neutralization of the solutions ranged from 1200μL to 3750μL. Samples of human enamel in the RB and RBL groups submitted to the acid challenge presented significantly decreased Knoop microhardness when compared with the group C. Conclusion All ED examined have potential to promote mineral loss due to the low pH and high titratable acidity. The ED analyzed promoted significant mineral losses on the dental enamel surface.

Influence of Platelet Membrane Sialic Acid and Platelet-Associated IgG on Ageing and Sequestration of Blood Platelets in Baboons

1993 ◽  
Vol 70 (04) ◽  
pp. 676-680 ◽  
Author(s):  
H F Kotzé ◽  
V van Wyk ◽  
P N Badenhorst ◽  
A du P Heyns ◽  
J P Roodt ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Life Span ◽  
Blood Platelets ◽  
Senescent Cell ◽  
Platelet Membrane ◽  
Antigen Complex ◽  
The Mean ◽  
Aggregation Of Platelets

SummaryPlatelets were isolated from blood of baboons and treated with neuraminidase to remove platelet membrane sialic acid, a process which artificially ages the platelets. The platelets were then labelled with 111In and their mean life span, in vivo distribution and sites of Sequestration were measured. The effect of removal of sialic acid on the attachment of immunoglobulin to platelets were investigated and related to the Sequestration of the platelets by the spleen, liver, and bone marrow. Removal of sialic acid by neuraminidase did not affect the aggregation of platelets by agonists in vitro, nor their sites of Sequestration. The removal of 0.51 (median, range 0.01 to 2.10) nmol sialic acid/108 platelets shortened their life span by 75 h (median, range 0 to 132) h (n = 19, p <0.001), and there was an exponential correlation between the shortening of the mean platelet life span and the amount of sialic acid removed. The increase in platelet-associated IgG was 0.112 (median, range 0.007 to 0.309) fg/platelet (n = 25, p <0.001) after 0.79 (median, range 0.00 to 6.70) nmol sialic acid/108 platelets was removed (p <0.001). There was an exponential correlation between the shortening of mean platelet life span after the removal of sialic acid and the increase in platelet-associated IgG. The results suggest that platelet membrane sialic acid influences ageing of circulating platelets, and that the loss of sialic acid may have exposed a senescent cell antigen that binds IgG on the platelet membrane. The antibody-antigen complex may then provide a signal to the macrophages that the platelet is old, and can be phagocytosed and destroyed.

EFFECT OF HUMAN ENAMEL ON WEAR OF THREE TYPES OF INTERIM RESTORATIVE MATERIALS (AN IN-VITRO STUDY)

2017 ◽  
Vol 63 (4) ◽  
pp. 3933-3945
Author(s):  
Khaled Haggag ◽  
Muhammad Abbas ◽  
Zainab EL Sharkawy
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Human Enamel ◽  
Restorative Materials

The Safety and Antimicrobial Properties of Stabilized Hypochlorous Acid in Acetic Acid Buffer for the Treatment of Acute Wounds—a Human Pilot Study and In Vitro Data

2021 ◽  
pp. 153473462110156
Author(s):  
Ewa A. Burian ◽  
Lubna Sabah ◽  
Klaus Kirketerp-Møller ◽  
Elin Ibstedt ◽  
Magnus M. Fazli ◽  
...  
Keyword(s):  
Pilot Study ◽  
Hypochlorous Acid ◽  
Treatment Time ◽  
Donor Site ◽  
Antimicrobial Properties ◽  
Prolonged Treatment ◽  
Wound Irrigation ◽  
The Mean ◽  
And Performance

Acute wounds may require cleansing to reduce the risk of infection. Stabilized hypochlorous acid in acetic buffer (HOCl + buffer) is a novel wound irrigation solution with antimicrobial properties. We performed a first-in-man, prospective, open-label pilot study to document preliminary safety and performance in the treatment of acute wounds. The study enrolled 12 subjects scheduled for a split-skin graft transplantation, where the donor site was used as a model of an acute wound. The treatment time was 75 s, given on 6 occasions. A total of 7 adverse events were regarded as related to the treatment; all registered as pain during the procedure for 2 subjects. One subject had a wound infection at the donor site. The mean colony-forming unit (CFU) decreased by 41% after the treatment, and the mean epithelialization was 96% on both days 14 (standard deviation [SD] 8%) and 21 (SD 10%). The study provides preliminary support for the safety, well-tolerance, and efficacy of HOCl + buffer for acute wounds. The pain was frequent although resolved quickly. Excellent wound healing and satisfying antimicrobial properties were observed. A subsequent in vitro biofilm study also indicated good antimicrobial activity against Pseudomonas aeruginosa with a 96% mean reduction of CFU, when used for a treatment duration of 15 min ( P < .0001), and a 50% decrease for Staphylococcus aureus ( P = .1010). Future larger studies are needed to evaluate the safety and performance of HOCl + buffer in acute wounds, including the promising antimicrobial effect by prolonged treatment on bacterial biofilms.

scholarly journals Formulation and in vitro evaluation of self-nanoemulsifying liquisolid tablets of furosemide

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Lena Dalal ◽  
Abdul Wahab Allaf ◽  
Hind El-Zein
Keyword(s):  
Theoretical Model ◽  
Castor Oil ◽  
In Vivo Studies ◽  
Coating Materials ◽  
Peg 400 ◽  
The Mean ◽  
Usp Apparatus ◽  
Solid System

AbstractSelf-nanoemulsifying drug delivery systems (SNEDDS) were used to enhance the dissolution rate of furosemide as a model for class IV drugs and the system was solidified into liquisolid tablets. SNEDDS of furosemide contained 10% Castor oil, 60% Cremophor EL, and 30% PEG 400. The mean droplets size was 17.9 ± 4.5 nm. The theoretical model was used to calculate the amounts of the carrier (Avicel PH101) and coating materials (Aerosil 200) to prepare liquisolid powder. Carrier/coating materials ratio of 5/1 was used and Ludipress was added to the solid system, thus tablets with hardness of 45 ± 2 N were obtained. Liquisolid tablets showed 2-folds increase in drug release as compared to the generic tablets after 60 min in HCl 0.1 N using USP apparatus-II. Furosemide loaded SNEDDS tablets have great prospects for further in vivo studies, and the theoretical model is useful for calculating the adequate amounts of adsorbents required to solidify these systems.

Sign in / Sign up

Export Citation Format

Share Document