scholarly journals Effects of Two In-Office Bleaching Agents with Different pH on the Structure of Human Enamel: An In Situ and In Vitro Study

2013 ◽  
Vol 38 (1) ◽  
pp. 100-110 ◽  
Author(s):  
Y Sa ◽  
L Sun ◽  
Z Wang ◽  
X Ma ◽  
S Liang ◽  
...  
Keyword(s):  
Artificial Saliva ◽  
In Vitro Study ◽  
Storage Condition ◽  
Human Saliva ◽  
Low Ph ◽  
Force Microscopy ◽  
Human Enamel ◽  
And Control

SUMMARY This study evaluated the effects of two in-office bleaching agents (Beyond and Opalescence Boost) with different pH on the structure and mechanical properties of human enamel in vitro and in situ. One hundred and eight enamel slabs were obtained from freshly extracted premolars. The specimens were randomly distributed into nine groups (n=12), and the human saliva (HS) in the volunteers' oral cavities was used to simulate the in situ condition: Beyond + HS, Opalescence Boost (O-Boost) + HS, Control + HS, Beyond + artificial saliva (AS), O-Boost + AS, Control + AS, Beyond + distilled water (DW), O-Boost + DW, and Control + DW. The bleaching treatments were performed on the first and eighth day, and the total bleaching time was 90 minutes. Baseline and final surface roughness (RMS), surface morphology, microhardness, and fracture toughness (FT) were measured before the treatment and on the fifteenth day, respectively. Compared with control groups, surface alterations on enamel were found in the Beyond + AS and Beyond + DW groups under atomic force microscopy evaluation. Two-way analysis of variance and Tukey test revealed that the RMS showed significant intergroup differences for both storage condition and bleaching agent, whereas microhardness and FT revealed no significant alteration. The results indicated that in-office bleaching agents with low pH values could induce enamel morphology alterations under in vitro conditions. The presence of natural HS could eliminate the demineralization effect caused by low pH.

scholarly journals Pellicle Modification with Casein and Mucin Does Not Affect Surface Loss from Erosion and Abrasion

2020 ◽  
Vol 54 (5-6) ◽  
pp. 509-516
Author(s):  
Tommy Baumann ◽  
Christoph Schmidt ◽  
Thiago Saads Carvalho
Keyword(s):  
In Vitro Study ◽  
Human Saliva ◽  
Ctrl Group ◽  
Protein Mixture ◽  
Surface Loss ◽  
Human Enamel ◽  
Optical Profilometer ◽  
And Control ◽  
Protein Treatment

<b><i>Aim:</i></b> A combination of the proteins casein and mucin is known to modify the salivary pellicle and improve its protection of the underlying enamel from erosion. It is so far not known if this protection is confined solely to erosion, or if it also extends to abrasion, and this in vitro study aimed at investigating this question. <b><i>Methods:</i></b> A total of 72 human enamel specimens were prepared and randomly assigned to four groups: pellicle (P), casein/mucin (CM), pellicle + casein/mucin (PCM), and control (Ctrl). Each specimen underwent five cycles, each cycle consisting of a pellicle/treatment part, an erosion part (3 min in 1% citric acid, pH 3.6, 25°C, 70 rpm), and an abrasion part (50 toothbrush strokes within 25 s in toothpaste slurry with a 200-g load). The pellicle/treatment part consisted of 2 h of incubation in whole human saliva for group P, 2 h of incubation (25°C, 70 rpm) in a protein mixture of 1% casein and 0.27% mucin for group CM, and 2 h of incubation in saliva followed by 2 h of incubation in the protein mixture for group PCM. The fourth group (Ctrl) served as the control and was kept in a humid chamber without saliva or protein treatment. The enamel surfaces were scanned with an optical profilometer initially and after the final cycle, and surface loss was analyzed. Furthermore, the surface microhardness (SMH) was measured initially, after each pellicle/treatment part and each erosion cycle, and after the final abrasion cycle. The results were analyzed with Kruskal-Wallis and Wilcoxon tests with Bonferroni corrections. <b><i>Results:</i></b> The different treatments did not show differences in surface loss and therefore did not protect enamel from surface loss by abrasion. Nonetheless, we observed differences in the SMH values, namely the Ctrl group being significantly softer than the experimental groups. <b><i>Conclusion:</i></b> The observed differences in SMH suggest that a different abrasion protocol could lead to differences in surface loss, and further investigation of whether and under which conditions pellicle modification leads to increased resistance to abrasion remains worthwhile.

scholarly journals Effect of a carbamide peroxide bleaching gel containing calcium or fluoride on human enamel surface microhardness

2005 ◽  
Vol 16 (2) ◽  
pp. 103-106 ◽  
Author(s):  
Rogério de Oliveira ◽  
Adriana Franco Paes Leme ◽  
Marcelo Giannini
Keyword(s):  
Artificial Saliva ◽  
In Vitro Study ◽  
Enamel Surface ◽  
Control Group ◽  
Carbamide Peroxide ◽  
Surface Microhardness ◽  
Treatment Groups ◽  
Peroxide Bleaching ◽  
Human Enamel

This in vitro study evaluated the surface microhardness of human enamel submitted to bleaching with 10% carbamide peroxide (CP) containing calcium or fluoride. Ninety-eight dental blocks (5 x 5 mm²) with polished enamel surfaces were randomly assigned to 7 treatment groups (n=14), as follows: without bleaching and storage in artificial saliva (control); 10% CP; 10% CP + 0.05% calcium; 10% CP + 0.1% calcium; 10% CP + 0.2% calcium; 10% CP + 0.2% fluoride; and 10% CP + 0.5% fluoride. During 14 days, enamel surfaces were daily exposed to a 6-h bleaching regimen followed by storage in artificial saliva. Surface microhardness was measured before (baseline), during (7th day), immediately after bleaching (14th day) and 1 week post bleaching. Data were analyzed by two-way ANOVA and Tukey's test (p<0.05). All treatments reduced SM significantly during the bleaching cycle (7th day), immediately after bleaching (14th day) and 1 week post bleaching, compared to baseline and to the unbleached control group. In conclusion, in spite of the addition of calcium and fluoride, all bleaching treatments affected the enamel surface microhardness.

scholarly journals Artificial Saliva Formulations versus Human Saliva Pretreatment in Dental Erosion Experiments

2016 ◽  
Vol 50 (1) ◽  
pp. 78-86 ◽  
Author(s):  
Graziela Ribeiro Batista ◽  
Carlos Rocha Gomes Torres ◽  
Beatrice Sener ◽  
Thomas Attin ◽  
Annette Wiegand
Keyword(s):  
Calcium Release ◽  
Artificial Saliva ◽  
Dental Erosion ◽  
Human Saliva ◽  
Deionized Water ◽  
Vitro Experiment ◽  
In Vitro Experiment ◽  
Calcium Loss

The aim of this study was to evaluate the erosion-preventive effect of different artificial saliva formulations and human saliva in vitro compared to human saliva in situ. In the in vitro experiment, bovine enamel and dentin specimens were stored in artificial saliva (4 different formulations, each n = 20), deionized water (n = 20) or human saliva (n = 6 enamel and dentin specimens/volunteer) for 120 min. In the in situ experiment, each of the 6 enamel and dentin specimens was worn intraorally by 10 volunteers for 120 min. The specimens were then eroded (HCl, pH 2.6, 60 s). Half of the specimens were subjected to microhardness analysis (enamel) and the determination of calcium release into the acid (enamel and dentin), while the other half were again placed in the respective medium or worn intraorally, respectively, for 120 min before a second erosion was performed. Knoop microhardness of enamel and the calcium release of enamel and dentin into the acid were again determined. Statistical analysis was conducted by two-way repeated-measures ANOVA or two-way ANOVA (α = 0.05). Enamel microhardness was not significantly different between all test groups after the first and the second erosive challenge, respectively. Enamel calcium loss was significantly lower in situ compared to the in vitro experiment, where there was no significant difference between all test groups. Dentin calcium loss was significantly lower than deionized water only after the first and than all except one artificial saliva after the second erosion. Under the conditions of this experiment, the use of artificial saliva formulations and human saliva in vitro does not reflect the intraoral situation in dental erosion experiments adequately.

scholarly journals Comparative evaluation of sorption and solubility of Amalgomer CR and Cention N restorative material- An in vitro study

2021 ◽  
Vol 5 (3) ◽  
pp. 122-125
Author(s):  
Krishna Prasada ◽  
◽  
Hithysh T Vidhyadhara ◽  
Keyword(s):  
Artificial Saliva ◽  
In Vitro Study ◽  
Low Ph ◽  
Vitro Study ◽  
Dietary Habit ◽  
Restorative Material ◽  
Group I ◽  
Restorative Materials ◽  
Carbonated Drinks

Introduction: The potential of direct restorative dental materials to resist masticatory force, without getting damaged and to remain unaffected after getting exposed to various media (chemical stability) in the mouth for a considerable period of time are important for their good clinical performance. Amalgomer CR is a ceramic reinforced posterior GIC having compressive, flexural and tensile strength close to that of amalgam. Cention N is a new material based on “alkasite” technology which is a subgroup of the composite resin. It is a novel bulk fill direct posterior restorative material. Every restorative material should meet the required ideal physical properties standards, among which water sorption and solubility are two important properties that influences the clinical durability of a restorative material. Chemical agents found in soft drinks and other beverages like coffee and tea can be exposed to restorative materials, continuously or intermittently. The modern dietary habit of frequent consumption of low pH carbonated drinks can alter the oral environment to an acidic range. Keeping all the above discussed factors in mind, this, in-vitro study was conducted to compare and evaluate the sorption and solubility of Cention N and Amalgomer CR in four direct solutions; Sprite, Pepsi, Coca Cola and artificial saliva. Aim: The aim and objective of this in-vitro study was to evaluate the sorption and solubility of Cention N and Amalgomer CR in four direct solutions; Sprite, Pepsi, Coca Cola and artificial saliva. Materials and methods: The materials that was used for this study are Cention N and Amalgomer CR. A total of forty samples of restorative material were prepared using a metallic mould and divided into two groups of twenty samples each (n=20); Group I: Cention N and Group II: Amalgomer CR. The weight before immersion in the test media (m1) was measured using digital analytical scale. Each group was subdivided into four subgroups (n=5) based on the test media they were immersed in; Subgroup 1: artificial saliva, Subgroup 2: Coco Cola, Subgroup 3: Pepsi, Subgroup 4: Sprite. Five specimens each of the test material were kept immersed in the test media for seven days. The weight of the samples after immersion (m2) in the test media after seven days and the final dry weight of the samples (m3) were measured. Sorption and solubility of Cention N and Amalgomer CR cement were calculated using ISO guidelines. Data was analyzed statistically using appropriate statistical tools. Results: In our study, both Amalgomer CR & Cention N showed values for sorption and solubility well under the values of ISO recommendations, however, Amalgomer CR had significantly more sorption and solubility when compared to Cention N. Among the test solutions used Sprite and Pepsi had the most degrading effect on Cention N and Amalgomer CR. Conclusion: The composition of material along with their setting / curing method and low pH carbonated drinks can influence the properties of direct esthetic restorative materials. In this present study, Cention N was marginally better when compared to Amalgomer CR.

The Impact of the Demineralized Organic Matrix on the Effect of TiF4 Varnish on the Progression of Dentin Erosive Loss

2017 ◽  
Vol 51 (3) ◽  
pp. 264-270 ◽  
Author(s):  
Beatriz Martines de Souza ◽  
Mariele Vertuan ◽  
Marília Afonso Rabelo Buzalaf ◽  
Ana Carolina Magalhães
Keyword(s):  
Citric Acid ◽  
Artificial Saliva ◽  
In Vitro Study ◽  
Organic Matrix ◽  
Analysis Data ◽  
Acid Ph ◽  
Bonferroni Test ◽  
And Control ◽  
The Impact

This in vitro study compared the effect of TiF4 varnish with that of NaF varnish, applied on pre-eroded bovine dentin samples, with respect to the progression of erosive loss, in the presence or absence of the demineralized organic matrix (DOM). One hundred and sixty bovine dentin samples were pre-eroded (0.1% citric acid, pH 2.5, 30 min). Half of the samples were subjected to the DOM removal (collagenase solution, 5 days). Samples with and without the DOM were treated according to the groups (n = 20 with DOM and 20 without DOM/group): TiF4 varnish (2.45% F), NaF varnish (2.45% F), placebo varnish (without fluoride) and control (no treatment). Thereafter, the treated samples were submitted to erosive challenges 4 × 90 s/day (0.1% citric acid, pH 2.5) during 7 days. Between the challenges, the samples were immersed in artificial saliva. The dentin erosive loss was measured using contact profilometry (µm, n = 15). Five dentin samples per group were prepared for energy-dispersive X-ray spectroscopy analysis. Data were compared using 2-way ANOVA/Bonferroni test (p < 0.05). Both fluoride varnishes were effective in reducing the erosive loss progression regardless of the dentin condition when compared to placebo varnish and control groups. Despite the fact that the TiF4 varnish was more effective than the NaF varnish for both dentin conditions (p < 0.001), its effect was significantly reduced in the absence of DOM (p < 0.05). It can be concluded that the TiF4 varnish is the best treatment in reducing the progression of dentin erosive loss (100%) in vitro, but its protective effect is more pronounced in the presence of DOM.

EFFECT OF HUMAN ENAMEL ON WEAR OF THREE TYPES OF INTERIM RESTORATIVE MATERIALS (AN IN-VITRO STUDY)

2017 ◽  
Vol 63 (4) ◽  
pp. 3933-3945
Author(s):  
Khaled Haggag ◽  
Muhammad Abbas ◽  
Zainab EL Sharkawy
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Human Enamel ◽  
Restorative Materials

scholarly journals In Vitro Effects of Resin Infiltration on Enamel Erosion Inhibition

2015 ◽  
Vol 40 (5) ◽  
pp. 492-502 ◽  
Author(s):  
GC Oliveira ◽  
AP Boteon ◽  
FQ Ionta ◽  
MJ Moretto ◽  
HM Honório ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Artificial Saliva ◽  
In Vitro Study ◽  
Adhesive System ◽  
Dental Erosion ◽  
Deep Penetration ◽  
Resin Infiltration ◽  
Fluorescence Confocal Microscopy ◽  
In Vitro Effects

SUMMARY Resin-based materials that show promising effects for preventing the progression of erosion have been studied. This in vitro study evaluated the effects of applying resin-based materials, including resin infiltration, on previously eroded enamel subjected to erosive challenges. The influence of enamel surface etching prior to application of the material was also studied. Bovine enamel blocks were immersed in hydrochloric acid (HCl), 0.01 M (pH 2.3), for 30 seconds in order to form a softened erosion lesion. The blocks were then randomly divided into nine groups (n=12) and treated as follows: C = control without treatment; Hel = pit & fissure resin sealant (Helioseal Clear); Adh = two-step self-etching adhesive system (AdheSe); Tet = two-step conventional adhesive system (Tetric N-bond); and Inf = infiltrant (Icon). The Helno, Adhno, Tetno, and Infno groups received the same materials without (or with no) surface conditioning. The depth of the material's penetration into softened erosion lesions was qualitatively analyzed using reflection and fluorescence confocal microscopy. After application of the materials, the blocks were immersed in HCl for two minutes; this step was followed by immersion in artificial saliva for 120 minutes four times a day for five days (erosive cycling). Both the enamel alteration and material thickness were analyzed using profilometry, and the results were submitted to Kruskal-Wallis and Dunn tests (p&gt;0.05). Images from the confocal microscopy showed minimal penetration of Adh/Adhno and deep penetration of Inf/Infno into the erosive lesions. The groups Hel, Adh, Inf, Tetno, and Infno resulted in the formation of a layer of material over the enamel, which was effective in inhibiting the progression of erosion. In conclusion, the infiltrant, with or without etching, was able to penetrate and protect the enamel against dental erosion. The other resin-based materials, except for the two-step conventional adhesive, were able to penetrate and inhibit the progression of erosive lesions only when they were applied after enamel etching.

scholarly journals Potential of different fluoride gels to prevent erosive tooth wear caused by gastroesophageal reflux

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Philipp Körner ◽  
Luca Georgis ◽  
Daniel B. Wiedemeier ◽  
Thomas Attin ◽  
Florian J. Wegehaupt
Keyword(s):  
Gastroesophageal Reflux ◽  
Artificial Saliva ◽  
In Vitro Study ◽  
Tooth Wear ◽  
Control Group ◽  
Erosive Tooth Wear ◽  
Custom Made ◽  
Post Hoc ◽  
Bovine Enamel

Abstract Background This in-vitro-study aimed to evaluate the potential of different fluoride gels to prevent gastroesophageal reflux induced erosive tooth wear. Methods Surface baseline profiles of a total of 50 bovine enamel specimens [randomly assigned to five groups (G1–5)] were recorded. All specimens were positioned in a custom made artificial oral cavity and perfused with artificial saliva (0.5 ml/min). Reflux was simulated 11 times a day during 12 h by adding HCl (pH 3.0) for 30 s (flow rate 2 ml/min). During the remaining 12 h (overnight), specimens were stored in artificial saliva and brushed twice a day (morning and evening) with a toothbrush and toothpaste slurry (15 brushing strokes). While specimens in the control group (G1) did not receive any further treatment, specimens in G2–5 were coated with different fluoride gels [Elmex Gelée (G2); Paro Amin Fluor Gelée (G3); Paro Fluor Gelée Natriumfluorid (G4); Sensodyne ProSchmelz Fluorid Gelée (G5)] in the evening for 30 s. After 20 days, surface profiles were recorded again and enamel loss was determined by comparing them with the baseline profiles. The results were statistically analysed using one-way analysis of variance (ANOVA) followed by Tukey`s HSD post-hoc test. Results The overall highest mean wear of enamel (9.88 ± 1.73 µm) was observed in the control group (G1), where no fluoride gel was applied. It was significantly higher (p < 0.001) compared to all other groups. G2 (5.03 ± 1.43 µm), G3 (5.47 ± 0.63 µm, p = 0.918) and G4 (5.14 ± 0.82 µm, p > 0.999) showed the overall best protection from hydrochloric acid induced erosion. Enamel wear in G5 (6.64 ± 0.86 µm) was significantly higher compared to G2 (p = 0.028) and G4 (p = 0.047). Conclusions After 20 days of daily application, all investigated fluoride gels are able to significantly reduce gastroesophageal reflux induced loss of enamel.

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