scholarly journals Toxicity of clove essential oil and its ester eugenyl acetate against Artemia salina

2017 ◽  
Vol 77 (1) ◽  
pp. 155-161 ◽  
Author(s):  
R. L. Cansian ◽  
A. B. Vanin ◽  
T. Orlando ◽  
S. P. Piazza ◽  
B. M. S. Puton ◽  
...  
Keyword(s):  
Essential Oil ◽  
Artemia Salina ◽  
Insect Vector ◽  
Enzymatic Esterification ◽  
Clove Oil ◽  
Eugenyl Acetate ◽  
Biological Potential ◽  
Insecticide Activity ◽  
Clove Essential Oil ◽  
High Doses

Abstract The production of compounds via enzymatic esterification has great scientific and technological interest due to the several inconveniences related to acid catalysis, mainly by these systems do not fit to the concept of “green chemistry”. Besides, natural products as clove oil present compounds with excellent biological potential. Bioactives compounds are often toxic at high doses. The evaluation of lethality in a less complex animal organism can be used to a monitoring simple and rapid, helping the identification of compounds with potential insecticide activity against larvae of insect vector of diseases. In this sense, the toxicity against Artemia salina of clove essential oil and its derivative eugenyl acetate obtained by enzymatic esterification using Novozym 435 as biocatalyst was evaluated. The conversion of eugenyl acetate synthesis was 95.6%. The results about the evaluation of toxicity against the microcrustacean Artemia salina demonstrated that both oil (LC50= 0.5993 µg.mL–1) and ester (LC50= 0.1178 µg.mL–1) presented high toxic potential, being the eugenyl acetate almost 5 times more toxic than clove essential oil. The results reported here shows the potential of employing clove oil and eugenyl acetate in insecticide formulations.

Antimicrobial and Antioxidant Activities of Clove Essential Oil and Eugenyl Acetate Produced by Enzymatic Esterification

2014 ◽  
Vol 174 (4) ◽  
pp. 1286-1298 ◽  
Author(s):  
Adriana B. Vanin ◽  
Tainara Orlando ◽  
Suelen P. Piazza ◽  
Bruna M. S. Puton ◽  
Rogério L. Cansian ◽  
...  
Keyword(s):  
Essential Oil ◽  
Antioxidant Activities ◽  
Enzymatic Esterification ◽  
Eugenyl Acetate ◽  
Clove Essential Oil

scholarly journals Clove Oil (Syzygium aromaticum L.) Activity against Alicyclobacillus acidoterrestris Biofilm on Technical Surfaces

Molecules ◽  
2020 ◽  
Vol 25 (15) ◽  
pp. 3334
Author(s):  
Alina Kunicka-Styczyńska ◽  
Agnieszka Tyfa ◽  
Dariusz Laskowski ◽  
Aleksandra Plucińska ◽  
Katarzyna Rajkowska ◽  
...  
Keyword(s):  
Essential Oil ◽  
Culture Conditions ◽  
Plate Count ◽  
Syzygium Aromaticum ◽  
Bacterial Cells ◽  
Clove Oil ◽  
Alicyclobacillus Acidoterrestris ◽  
Agitated Culture ◽  
Clove Essential Oil ◽  
Biofilm Development

Acidotermophilic bacteria Alicyclobacillus acidoterrestris is one of the main contaminants in the fruit industry forming biofilms which are difficult to remove from the production line by conventional methods. An alternative approach aims for the use of essential oils to prevent Alicyclobacillus biofilm development. The effect of clove essential oil on A. acidoterrestris biofilms on glass and polyvinyl chloride surfaces under static and agitated culture conditions was investigated by atomic force microscopy and the plate count method. The medium-flow and the type of technical surface significantly influenced A. acidoterrestris biofilm. The PVC was colonized in a greater extent comparing to glass. Clove essential oil in 0.05% (v/v) caused 25.1–65.0% reduction of biofilms on the technical surfaces along with substantial changes in their morphology by a decrease in the biofilm: height, surface roughness, and surface area difference. The oil also induced alteration in individual bacterial cells length and visible increase of their roughness. Clove essential oil seems to release EPS from biofilm and thus induce detachment of bacteria from the surface. Due to anti-A. acidoterrestris biofilm activity, the clove oil may be used in the juice industry to hinder a development of A. acidoterrestris biofilms on production surfaces.

scholarly journals Antifungal activity of the clove essential oil from Syzygium aromaticum on Candida, Aspergillus and dermatophyte species

2009 ◽  
Vol 58 (11) ◽  
pp. 1454-1462 ◽  
Author(s):  
Eugénia Pinto ◽  
Luís Vale-Silva ◽  
Carlos Cavaleiro ◽  
Lígia Salgueiro
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Cell Membrane ◽  
Fungal Infections ◽  
Yeast Cells ◽  
Syzygium Aromaticum ◽  
Clove Oil ◽  
Fungal Cell ◽  
Minimum Fungicidal Concentration ◽  
Clove Essential Oil

The composition and antifungal activity of clove essential oil (EO), obtained from Syzygium aromaticum, were studied. Clove oil was obtained commercially and analysed by GC and GC-MS. The EO analysed showed a high content of eugenol (85.3 %). MICs, determined according to Clinical and Laboratory Standards Institute protocols, and minimum fungicidal concentration were used to evaluate the antifungal activity of the clove oil and its main component, eugenol, against Candida, Aspergillus and dermatophyte clinical and American Type Culture Collection strains. The EO and eugenol showed inhibitory activity against all the tested strains. To clarify its mechanism of action on yeasts and filamentous fungi, flow cytometric and inhibition of ergosterol synthesis studies were performed. Propidium iodide rapidly penetrated the majority of the yeast cells when the cells were treated with concentrations just over the MICs, meaning that the fungicidal effect resulted from an extensive lesion of the cell membrane. Clove oil and eugenol also caused a considerable reduction in the quantity of ergosterol, a specific fungal cell membrane component. Germ tube formation by Candida albicans was completely or almost completely inhibited by oil and eugenol concentrations below the MIC values. The present study indicates that clove oil and eugenol have considerable antifungal activity against clinically relevant fungi, including fluconazole-resistant strains, deserving further investigation for clinical application in the treatment of fungal infections.

scholarly journals Experience of using clove oil for olfactory enrichment in Russian Zoos

2019 ◽  
Vol 9 (3) ◽  
pp. 381-383
Author(s):  
T. A. Karmanova ◽  
D. D. Volgina ◽  
T. V. Antonenko ◽  
A. V. Matsyura
Keyword(s):  
Essential Oil ◽  
Snow Leopard ◽  
Clove Oil ◽  
Stereotypical Behavior ◽  
Clove Essential Oil ◽  
Far Eastern ◽  
African Lions ◽  
Red Wolves

Successful experiments on olfactory enrichment of clove oil in Russian zoos have been carried out. Olfactory enrichment with clove oil reduced or eliminated stereotypical behavior (pacing) in most Amur tigers, African lions, and some Canadian and Red wolves. The behavior of the Far Eastern leopard and the Snow leopard behavior has not changed significantly under the influence of clove essential oil. Olfactory enrichment of clove essential oil is more successful for young animals.

scholarly journals Syzygium aromaticum Essential Oil to the Control of Tenuipalpus heveae

2019 ◽  
Vol 11 (8) ◽  
pp. 295
Author(s):  
Thalia Mayara Pereira de Melo ◽  
Eliamara Marques da Silva ◽  
Abimael Gomes da Silva ◽  
Gustavo Haralampidou da Costa Vieira ◽  
Beatriz Garcia Lopes
Keyword(s):  
Regression Analysis ◽  
Essential Oil ◽  
Essential Oils ◽  
Syzygium Aromaticum ◽  
Clove Oil ◽  
Adult Females ◽  
Clove Essential Oil ◽  
Petri Dishes ◽  
Adult Mite ◽  
Mite Mortality

The objective was to determine the effect of essential oils on the mortality and repellency of the Tenuipalpus heveae Baker mite (Acari, Tenuipalpidae). Leaf discs of rubber trees were deposited in Petri dishes containing a moistened cotton layer. Posteriorly, the essential oils were diluted to formulate solutions, which were sprayed onto the discs. The experimental design was completely randomized, in a 3 × 4 factorial design: neem, cedar and clove oils at doses 0, 10 μL, 50 μL and 100 μL, with five replicates, with ten adult females being considered per replicate. The exposure to the effect of essential oil was evaluated after 24, 48, 72 and 96 hours for the number of females dead and females in the cotton. The data were submitted to analysis of variance, the values were transformed into (x + 0.5)1/2, and the means were grouped by the Scott-Knot test at 5% probability for the treatments, the doses were submitted to regression analysis. From the 48 hours of evaluation, clove oil showed greater potential for adult mite mortality, being 1.38 in 48 hours, 1.40 in 72 hours and 1.50 in 96 hours. The dose of 100 μL presented a greater potential for the neem and clove oils. The clove essential oil has potential in the control of Tenuipalpus heveae.

scholarly journals Toxicity and larvicidal activity on Aedes aegypti of citronella essential oil submitted to enzymatic esterification

2023 ◽  
Vol 83 ◽  
Author(s):  
R. L. Cansian ◽  
A. Staudt ◽  
J. L. Bernardi ◽  
B. M. S. Puton ◽  
D. Oliveira ◽  
...  
Keyword(s):  
Essential Oil ◽  
Aedes Aegypti ◽  
Cinnamic Acid ◽  
Biological Activities ◽  
Artemia Salina ◽  
Enzymatic Esterification ◽  
Conversion Rates ◽  
Before And After ◽  
Natural Pesticides ◽  
Acid Esters

Abstract The essential oil of citronella (Cymbopogon winterianus) has several biological activities, among them the insect repellent action. Some studies showed that cinnamic acid esters can be applied as natural pesticides, insecticides and fungicides. In this context, the objective of the present work was to evaluate the production of esters from citronella essential oil with cinnamic acid via enzymatic esterification. Besides, the essential oil toxicity before and after esterification against Artemia salina and larvicidal action on Aedes aegypti was investigated. Esters were produced using cinnamic acid as the acylating agent and citronella essential oil (3:1) in heptane and 15 wt% NS 88011 enzyme as biocatalysts, at 70 °C and 150 rpm. Conversion rates of citronellyl and geranyl cinnamates were 58.7 and 69.0% for NS 88011, respectively. For the toxicity to Artemia salina LC50 results of 5.29 μg mL-1 were obtained for the essential oil and 4.36 μg mL-1 for the esterified oils obtained with NS 88011. In the insecticidal activity against Aedes aegypti larvae, was obtained LC50 of 111.84 μg mL-1 for the essential oil of citronella and 86.30 μg mL-1 for the esterified oils obtained with the enzyme NS 88011, indicating high toxicity of the esters. The results demonstrated that the evaluated samples present potential of application as bioinsecticide.

REVIEW : KARAKTERISTIK FISIK DAN DAYA IRITASI MINYAK ATSIRI BUNGA CENGKEH (Syzygium aromaticum) DALAM BEBERAPA TIPE BASIS SEBAGAI ANTI-INFLAMASI

2020 ◽  
Vol 5 (2) ◽  
pp. 364-375
Author(s):  
Amraini Amelia ◽  
◽  
Nining Sugihartini ◽  
Hari Susanti ◽  
Keyword(s):  
Essential Oil ◽  
Physical Properties ◽  
Research Method ◽  
Water Soluble ◽  
Syzygium Aromaticum ◽  
Absorption Properties ◽  
Inflammatory Drugs ◽  
Clove Essential Oil ◽  
Soluble Base ◽  
Essential Oil Formulations

This review aims to determine the types of bases that can be used every day, which are effective and efficient as anti-inflammatory drugs. The research method used was to review the development of clove essential oil formulations that have been carried out using various concentrations of various types of bases including M / A type cream, A / M type cream, water soluble base, lotion, emulgel, hydrocarbons, hydrogels and absorbents. The recommended formulation is type M / A cream with a concentration of 5% clove flower essential oil. The types of bases studied were M / A cream, type A / M cream, water soluble base, lotion, emulgel, hydrocarbons, hydrogels and absorption properties which had good physical properties and did not irritate the skin of the test animals. This review refers to several national and international journals released in the last ten years, from 2010 to 2020.

Extraction and Quantification of Eugenol from Clove Buds Using HPLC

2020 ◽  
Vol 7 (1) ◽  
pp. 17-23
Author(s):  
Khadiza Fitri Shafira ◽  
Abul Kalam Azad ◽  
Zubair Khalid Labu ◽  
Abul Bashar Mohammed Helal Uddin
Keyword(s):  
Processing Time ◽  
Soxhlet Extraction ◽  
Extraction Methods ◽  
Past Study ◽  
Solvent Ratio ◽  
Clove Oil ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Significant Difference ◽  
Clove Essential Oil

Background: Eugenol is the main constituent of clove essential oil. Past studies have found that clove oil has diverse uses in the pharmaceutical field due to its antioxidant, antibacterial and anesthetic properties. Objective: This work compares the performance of different extraction methods and factors and identifies the effect of the treatments on oil yields and eugenol content. Materials and Methods: Maceration, Hydro distillation, microwave-assisted extraction (MAE), and Soxhlet were performed. The best technique was identified according to yield and content. Further studies were conducted to examine the effects of different factors, such as solvent types (ethanol and methanol) and sample-to-solvent ratio (1:10 and 1:15). HPLC UV-Vis was utilized in the analysis of eugenol concentration. Results and Discussion: Soxhlet extraction provided the highest yield (39.98%) and eugenol content (15.83%), compared to other methods. The results observed from several Soxhlet extraction factors showed that there is no significant difference between the different factors. In the meantime, methanol 1:15 provided the greatest amount of yields (57.83%) and eugenol content (22.21%). In this regard, the higher ratio resulted in higher eugenol content. Conclusion: The results obtained are less comparable because the processing time, the working solvent, and the separation technique were carried out differently for each method. In the meantime, as there is no past study that compared the selected methods and factors, this study’s findings will contribute substantially to fill the gap in this field.

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