scholarly journals Clove Oil (Syzygium aromaticum L.) Activity against Alicyclobacillus acidoterrestris Biofilm on Technical Surfaces

Molecules ◽  
2020 ◽  
Vol 25 (15) ◽  
pp. 3334
Author(s):  
Alina Kunicka-Styczyńska ◽  
Agnieszka Tyfa ◽  
Dariusz Laskowski ◽  
Aleksandra Plucińska ◽  
Katarzyna Rajkowska ◽  
...  
Keyword(s):  
Essential Oil ◽  
Culture Conditions ◽  
Plate Count ◽  
Syzygium Aromaticum ◽  
Bacterial Cells ◽  
Clove Oil ◽  
Alicyclobacillus Acidoterrestris ◽  
Agitated Culture ◽  
Clove Essential Oil ◽  
Biofilm Development

Acidotermophilic bacteria Alicyclobacillus acidoterrestris is one of the main contaminants in the fruit industry forming biofilms which are difficult to remove from the production line by conventional methods. An alternative approach aims for the use of essential oils to prevent Alicyclobacillus biofilm development. The effect of clove essential oil on A. acidoterrestris biofilms on glass and polyvinyl chloride surfaces under static and agitated culture conditions was investigated by atomic force microscopy and the plate count method. The medium-flow and the type of technical surface significantly influenced A. acidoterrestris biofilm. The PVC was colonized in a greater extent comparing to glass. Clove essential oil in 0.05% (v/v) caused 25.1–65.0% reduction of biofilms on the technical surfaces along with substantial changes in their morphology by a decrease in the biofilm: height, surface roughness, and surface area difference. The oil also induced alteration in individual bacterial cells length and visible increase of their roughness. Clove essential oil seems to release EPS from biofilm and thus induce detachment of bacteria from the surface. Due to anti-A. acidoterrestris biofilm activity, the clove oil may be used in the juice industry to hinder a development of A. acidoterrestris biofilms on production surfaces.

scholarly journals Antifungal activity of the clove essential oil from Syzygium aromaticum on Candida, Aspergillus and dermatophyte species

2009 ◽  
Vol 58 (11) ◽  
pp. 1454-1462 ◽  
Author(s):  
Eugénia Pinto ◽  
Luís Vale-Silva ◽  
Carlos Cavaleiro ◽  
Lígia Salgueiro
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Cell Membrane ◽  
Fungal Infections ◽  
Yeast Cells ◽  
Syzygium Aromaticum ◽  
Clove Oil ◽  
Fungal Cell ◽  
Minimum Fungicidal Concentration ◽  
Clove Essential Oil

The composition and antifungal activity of clove essential oil (EO), obtained from Syzygium aromaticum, were studied. Clove oil was obtained commercially and analysed by GC and GC-MS. The EO analysed showed a high content of eugenol (85.3 %). MICs, determined according to Clinical and Laboratory Standards Institute protocols, and minimum fungicidal concentration were used to evaluate the antifungal activity of the clove oil and its main component, eugenol, against Candida, Aspergillus and dermatophyte clinical and American Type Culture Collection strains. The EO and eugenol showed inhibitory activity against all the tested strains. To clarify its mechanism of action on yeasts and filamentous fungi, flow cytometric and inhibition of ergosterol synthesis studies were performed. Propidium iodide rapidly penetrated the majority of the yeast cells when the cells were treated with concentrations just over the MICs, meaning that the fungicidal effect resulted from an extensive lesion of the cell membrane. Clove oil and eugenol also caused a considerable reduction in the quantity of ergosterol, a specific fungal cell membrane component. Germ tube formation by Candida albicans was completely or almost completely inhibited by oil and eugenol concentrations below the MIC values. The present study indicates that clove oil and eugenol have considerable antifungal activity against clinically relevant fungi, including fluconazole-resistant strains, deserving further investigation for clinical application in the treatment of fungal infections.

scholarly journals Syzygium aromaticum Essential Oil to the Control of Tenuipalpus heveae

2019 ◽  
Vol 11 (8) ◽  
pp. 295
Author(s):  
Thalia Mayara Pereira de Melo ◽  
Eliamara Marques da Silva ◽  
Abimael Gomes da Silva ◽  
Gustavo Haralampidou da Costa Vieira ◽  
Beatriz Garcia Lopes
Keyword(s):  
Regression Analysis ◽  
Essential Oil ◽  
Essential Oils ◽  
Syzygium Aromaticum ◽  
Clove Oil ◽  
Adult Females ◽  
Clove Essential Oil ◽  
Petri Dishes ◽  
Adult Mite ◽  
Mite Mortality

The objective was to determine the effect of essential oils on the mortality and repellency of the Tenuipalpus heveae Baker mite (Acari, Tenuipalpidae). Leaf discs of rubber trees were deposited in Petri dishes containing a moistened cotton layer. Posteriorly, the essential oils were diluted to formulate solutions, which were sprayed onto the discs. The experimental design was completely randomized, in a 3 × 4 factorial design: neem, cedar and clove oils at doses 0, 10 μL, 50 μL and 100 μL, with five replicates, with ten adult females being considered per replicate. The exposure to the effect of essential oil was evaluated after 24, 48, 72 and 96 hours for the number of females dead and females in the cotton. The data were submitted to analysis of variance, the values were transformed into (x + 0.5)1/2, and the means were grouped by the Scott-Knot test at 5% probability for the treatments, the doses were submitted to regression analysis. From the 48 hours of evaluation, clove oil showed greater potential for adult mite mortality, being 1.38 in 48 hours, 1.40 in 72 hours and 1.50 in 96 hours. The dose of 100 μL presented a greater potential for the neem and clove oils. The clove essential oil has potential in the control of Tenuipalpus heveae.

REVIEW : KARAKTERISTIK FISIK DAN DAYA IRITASI MINYAK ATSIRI BUNGA CENGKEH (Syzygium aromaticum) DALAM BEBERAPA TIPE BASIS SEBAGAI ANTI-INFLAMASI

2020 ◽  
Vol 5 (2) ◽  
pp. 364-375
Author(s):  
Amraini Amelia ◽  
◽  
Nining Sugihartini ◽  
Hari Susanti ◽  
Keyword(s):  
Essential Oil ◽  
Physical Properties ◽  
Research Method ◽  
Water Soluble ◽  
Syzygium Aromaticum ◽  
Absorption Properties ◽  
Inflammatory Drugs ◽  
Clove Essential Oil ◽  
Soluble Base ◽  
Essential Oil Formulations

This review aims to determine the types of bases that can be used every day, which are effective and efficient as anti-inflammatory drugs. The research method used was to review the development of clove essential oil formulations that have been carried out using various concentrations of various types of bases including M / A type cream, A / M type cream, water soluble base, lotion, emulgel, hydrocarbons, hydrogels and absorbents. The recommended formulation is type M / A cream with a concentration of 5% clove flower essential oil. The types of bases studied were M / A cream, type A / M cream, water soluble base, lotion, emulgel, hydrocarbons, hydrogels and absorption properties which had good physical properties and did not irritate the skin of the test animals. This review refers to several national and international journals released in the last ten years, from 2010 to 2020.

Syzygium aromaticum (Clove) essential oil: an alternative for the sanitization of citrus fruit in packinghouses

2021 ◽  
Author(s):  
Tiago Soraggi Battagin ◽  
Mario Nicolas Caccalano ◽  
Guilherme Dilarri ◽  
Caio Felipe Cavicchia Zamuner ◽  
Natália Alleoni ◽  
...  
Keyword(s):  
Essential Oil ◽  
Citrus Fruit ◽  
Syzygium Aromaticum ◽  
Clove Essential Oil

Enzyme‐assisted extraction of essential oils from Syzygium aromaticum

2011 ◽  
Vol 1 (6) ◽  
pp. 248-254
Author(s):  
R. Amudan ◽  
D. V. Kamat ◽  
S. D. Kamat
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Steam Distillation ◽  
Antibacterial Effect ◽  
Chemical Extraction ◽  
Syzygium Aromaticum ◽  
Clove Oil ◽  
Zone Size ◽  
Structure Quality ◽  
Assisted Extraction

The essential oil of clove ( Syzygium aromaticum) holds an important positionamongst widely used essential oils. A typical steam distillation processfor the extraction of clove oil provides a 10.1% yield. Recent studies involvedthe use of enzymes such as pectinase, amylase, lignocellulase, and cellulaseon the powder of clove buds, prior to extraction. The traditional methods ofphysical and chemical extraction are effective but may affect the structure,quality and yield of the phytochemicals extracted. In the current study,hence, enzymes specific for action on the cell wall have been used in the pretreatment prior to extraction, to enhance the quality and yield of the phytochemicalsextracted. The results indicated that all the enzymes, gave morethan 50% higher yield than control in terms of weight of extracted essentialoil. A mixture of the enzymes gave the highest yield of 17.82%. Gas chromatographyresults indicated that the essential oil extracted using amylase hada maximum eugenol content of 70%, in comparison with the eugenol content(62–68%) in the essential oils extracted using the rest of the enzymes.Antibacterial activity of all the extracts was studied on methicillin â€resistantStaphylococcus aureus  (MRSA). The essential oil extracted by using amylaseinhibitedMRSA, showed a zone size of 40 mm, whereas the essential oil extractedby using lignocellulase showed a zone size of 45 mm. The gas chromatogramindicated the maximum number of peaks in this extract, whichcould be producing a combined antibacterial effect on the organism. Thespecific gravity values of the essential oil extracted using lignocellulase andamylase was 1.051 and 1.062, respectively, whereas the control had a specificgravity of 1.015.

scholarly journals Development of a Millet Starch Edible Film Containing Clove Essential Oil

Foods ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 184 ◽  
Author(s):  
Alaa G. Al-Hashimi ◽  
Altemimi B. Ammar ◽  
Lakshmanan G. ◽  
Francesco Cacciola ◽  
Naoufal Lakhssassi
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Antioxidant Properties ◽  
Control Sample ◽  
Barrier Properties ◽  
Edible Film ◽  
Gas Chromatography Mass Spectrometry ◽  
Syzygium Aromaticum ◽  
Preservation Method ◽  
Clove Essential Oil

Medicinal plants contain various secondary metabolites. The present study analyzed the essential oil of buds from clove (Syzygium aromaticum L.; Family: Myrtaceae) using gas chromatography-mass spectrometry (GC-MS). GC-MS analysis showed the presence of six major phytoconstituents, such as eugenol (66.01%), caryophyllene (19.88%), caryophyllene oxide (5.80%), phenol, 2-methoxy-4-(2-propenyl)-acetate (4.55%), and humulene (3.75%). The effect of clove essential oils (CEO) at 0%, 1%, 2%, and 3% (w/w) on the mechanical and barrier properties of starch films was evaluated. The tensile strength (TS) and elongation (E) of films with clove essential oil were 6.25 ± 0.03 MPa and 5.67% ± 0.08%, respectively. The antioxidant activity of the films significantly increased the millet starch film and presented the lowest antioxidant activity (0.3%) at a 30 minute incubation for the control sample, while increasing CEO fraction in the starch film lead to an increase in antioxidant activity, and the 3% CEO combined film presented the highest antioxidant activity (15.96%) at 90 min incubation. This finding could be explained by the incorporation of clove oil containing antioxidant properties that significantly increased with the incorporation of CEO (p < 0.05). A zone of inhibition ranging from 16 to 27 mm in diameter was obtained when using a concentration of CEO ranging from 1% to 3%. We also observed the presence of an antimicrobial activity on several tested microorganism including Escherichia coli, Pseudomonas aeruginosa, Enterobacter sp, Bacillus cereus, Staphylococcus aureus, and Trichoderma fungi. Thus, the current study reveals the possibility of using a millet starch edible film as a preservation method.

scholarly journals Insecticide activity of clove essential oil on bean weevil and maize weevil

2016 ◽  
Vol 20 (1) ◽  
pp. 72-77 ◽  
Author(s):  
Carlos F. Jairoce ◽  
Cristiano M. Teixeira ◽  
Camila F. P. Nunes ◽  
Adrise M. Nunes ◽  
Claudio M. P. Pereira ◽  
...  
Keyword(s):  
Essential Oil ◽  
Integrated Management ◽  
Storage Conditions ◽  
Chemical Components ◽  
Syzygium Aromaticum ◽  
Bean Weevil ◽  
Maize Weevil ◽  
Promising Alternative ◽  
Stored Grains ◽  
Clove Essential Oil

ABSTRACT Bean weevil and maize weevil can cause considerable damage to stored grains. These insects are mainly controlled with synthetic chemical insecticides, which may bring serious problems to human and environmental health. Therefore, this study aimed to evaluate the efficiency of the essential oil of clove [Syzygium aromaticum (L.) Merrill & Perry (Myrtaceae) (origin: Bahia, season Sep.2014-Feb.2015)] in the control of S. zeamais and A. obtectus under laboratory conditions. The essential oil was extracted through the classic hydrodistillation process and its chemical components were identified via gas chromatography. Oil efficiency was tested at the doses of 35, 17.9, 8.9, 3.6, 1.8, 0.4 and 0.2 μL g-1 (derived from a pilot study) for insect control and the LC50 was determined. The results showed that eugenol was the major compound. The essential oil caused mortality of 100% for both species 48 h after treatment with the concentrations of 17.9 and 35 μL g-1. The LC50 for A. obtectus was 9.45 μL g-1, against 10.15 μL g-1 for S. zeamais. The use of clove essential oil represents a promising alternative to be used under storage conditions for the integrated management of stored grains pests.

scholarly journals Clove Essential Oil (Syzygium aromaticum L. Myrtaceae): Extraction, Chemical Composition, Food Applications and Essential Bioactivities for Human Health

2021 ◽  
Author(s):  
José Nabor Haro-González ◽  
Gustavo Adolfo Castillo-Herrera ◽  
Moisés Martínez-Velázquez ◽  
Hugo Espinosa-Andrews
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Human Health ◽  
Biological Activities ◽  
Syzygium Aromaticum ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Food Applications ◽  
Clove Essential Oil ◽  
Interest In Research

Clove (Syzygium aromaticum L. Myrtaceae) is an aromatic plant widely cultivated in tropical and subtropical countries, rich in volatile compounds and antioxidants such as eugenol, &beta;-caryophyllene, and &alpha;-humulene. Clove essential oil has received considerable interest due to its wide application in the perfumery, cosmetic, health, medical, flavoring, and food industries. Clove essential oil has relevant biological activities to human health, including antimicrobial, antioxidant, and insecticide. This review describes the effect of the extraction method (hydrodistillation, steam distillation, ultrasound-assisted extraction, microwave-assisted extraction, cold pressing, and supercritical fluid extraction) on the chemical composition of essential oil and its correlation with their biological activities. Likewise, are summarized the main compounds and their reported biological activities. Furthermore, the main applications in clove essential oil in the food industry are presented. Finally, this review presents the new biological activities such as anti-inflammatory, analgesic, anesthetic, antinociceptive and anticancer, which are beneficial for human health. This review aims to compile the effect of different methods of extracting clove essential oil on chemical composition, food applications, as well as a current description of biological activities of interest to human health. Biological activities have increased interest in research into this essential oil and its future applications in the food or pharmaceutical industry.

scholarly journals Experience of using clove oil for olfactory enrichment in Russian Zoos

2019 ◽  
Vol 9 (3) ◽  
pp. 381-383
Author(s):  
T. A. Karmanova ◽  
D. D. Volgina ◽  
T. V. Antonenko ◽  
A. V. Matsyura
Keyword(s):  
Essential Oil ◽  
Snow Leopard ◽  
Clove Oil ◽  
Stereotypical Behavior ◽  
Clove Essential Oil ◽  
Far Eastern ◽  
African Lions ◽  
Red Wolves

Successful experiments on olfactory enrichment of clove oil in Russian zoos have been carried out. Olfactory enrichment with clove oil reduced or eliminated stereotypical behavior (pacing) in most Amur tigers, African lions, and some Canadian and Red wolves. The behavior of the Far Eastern leopard and the Snow leopard behavior has not changed significantly under the influence of clove essential oil. Olfactory enrichment of clove essential oil is more successful for young animals.

scholarly journals Effect of Clove Essential Oil (Syzygium aromaticum) on Some Virulence Factors of Staphylococcus aureus

2020 ◽  
Vol 14 (4) ◽  
pp. 13-19
Author(s):  
Azizollah Ebrahimi ◽  
Soheila Rabiaee ◽  
Sharareh Lotfalian ◽  
Saied Habibian ◽  
◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Virulence Factors ◽  
Syzygium Aromaticum ◽  
Clove Essential Oil
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