scholarly journals Evaluation of platelet-rich plasma gel as an angiogenesis-inducing agent in canineadvancement skin flaps

2020 ◽  
Vol 40 (6) ◽  
pp. 474-478
Author(s):  
Grazielle A.S. Aleixo ◽  
Maria C.O.C. Coelho ◽  
Telga L.A. Almeida ◽  
Márcia F. Pereira ◽  
Miriam N. Teixeira ◽  
...  
Keyword(s):  
Blood Vessel ◽  
Skin Color ◽  
Platelet Rich Plasma ◽  
Skin Flap ◽  
Skin Flaps ◽  
Experimental Conditions ◽  
Significant Difference ◽  
Experimental Group ◽  
Prp Gel ◽  
Made In

ABSTRACT: This work aimed to evaluate the effect of platelet-rich plasma (PRP) on advancement skin flaps in dogs regarding improvement of vascularization, with focus on increasing its viable area, since there are reports that it is a potential angiogenesis stimulator. The experimental group was composed of eight adult bitches, in which two advancement skin flaps were made in the ventral abdominal region. No product was applied in the control flap (CF), while PRP was used in the contralateral flap, called treated flap (TF). The areas were clinically evaluated every two days until the 7th postoperative day regarding skin color and presence of necrosis. At 10 days, both flaps were removed and submitted to histological examination and blood vessel morphometry. The vessels counted in each group were statistically analyzed by the F-test at 1% probability. Results showed no significant difference in macroscopic changes in the wound, or CF and TF vascularization, thus suggesting that PRP gel did not improve advancement skin flap angiogenesis in bitches under the experimental conditions in which this research was developed.

The Relationship of Hematocrit Levels to Skin Flap Survival Length in the Pig

1981 ◽  
Vol 89 (5) ◽  
pp. 750-752 ◽  
Author(s):  
Jeffrey B. Alperstein ◽  
Howard L. Levine ◽  
Harvey M. Tucker
Keyword(s):  
Human Skin ◽  
Control Animal ◽  
Skin Flap ◽  
Random Pattern ◽  
Skin Flaps ◽  
Flap Survival ◽  
Significant Difference ◽  
Skin Flap Survival ◽  
Relationship Of ◽  
The Relationship

The work of several investigators suggests that anemia may increase the survival length of skin flaps in the dog and the rabbit. The following experiment was designed to study the survival of standardized skin flaps of varying lengths in normocythemic, polycythemic, and anemic pigs. The pig was chosen because of the similarity of its skin to that of human skin. Twenty-nine standardized random-pattern flaps and six standardized arterial flaps were studied in pigs with varying hematocrits. A statistically significant increase was found in the survival lengths of skin flaps in the polycythemic animal as compared with the anemic one. No significant difference was found when the flap survival lengths of the normocythemic control animal were compared with those of the polycythemic animal or with those of the anemic animal. These findings suggest that relative polycythemia may allow improved flap length-survival and, in contradistinction to the findings of previous investigators, that anemia does not result in improved survival length.

Locally injected autologous platelet-rich plasma enhanced tissue perfusion and improved survival of long subdermal plexus skin flaps in dogs

2014 ◽  
Vol 27 (05) ◽  
pp. 379-386 ◽  
Author(s):  
L. G. Papazoglou ◽  
P. Loukopoulos ◽  
G. Kazakos ◽  
A. Chantes ◽  
N. Giannakas ◽  
...  
Keyword(s):  
Platelet Rich Plasma ◽  
Skin Flap ◽  
Major Complication ◽  
Tissue Perfusion ◽  
Width Ratio ◽  
Skin Flaps ◽  
Doppler Flowmetry ◽  
Percentage Survival ◽  
Skin Flap Survival ◽  
Autologous Platelet Rich Plasma

Summary Objectives: Distal flap necrosis remains a major complication in subdermal plexus (random) skin flaps. Platelet-rich plasma (PRP) has been shown to improve the survival of ischemic random skin flaps in rats. The objective of this study was to evaluate the effect of locally injected autologous PRP on the survival of long (5:1 length-to-width ratio) subdermal plexus skin flaps in dogs. Methods: A 2x10 cm subdermal plexus skin flap was created bilaterally on the abdominal wall of six Beagle dogs. One randomly selected side received 2.5 ml of fresh autologous PRP injected evenly between sutures underneath the flap, whereas the other side was left untreated (control). Skin flap survival was evaluated macroscopically, histologically and by laser-Doppler flowmetry measurements of tissue perfusion. Results: Flap percentage survival on day 10 (96.3% versus 74.5%; p = 0.046) and tissue perfusion (p <0.036) were significantly higher in PRP-treated flaps compared with controls. Histologically, there was less oedema in PRP-treated flaps compared to controls (p = 0.01), whereas collagen production and angiogenesis did not differ significantly between the two groups. Clinical significance: The use of locally injected autologous PRP increases tissue perfusion and improves the survival of long subdermal plexus skin flaps in dogs.

scholarly journals Effects of hyperbaric oxygen and N-acetylcysteine in survival of random pattern skin flaps in rats

2012 ◽  
Vol 45 (03) ◽  
pp. 453-458 ◽  
Author(s):  
Fernando da Rocha ◽  
Djalma Fagundes ◽  
Jefferson Pires ◽  
Fernanda Salim da Rocha
Keyword(s):  
Hyperbaric Oxygen ◽  
Skin Flap ◽  
Control Area ◽  
Random Pattern ◽  
Skin Flaps ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Flap Design ◽  
A Site

ABSTRACT Objective: Our aim is to investigate the role of HBO (hyperbaric oxygen), NAC (N-acetylcysteine), and HBO plus NAC on the necrosis area of random rat′s skin flaps of a modified McFarlane flap design. Materials and Methods: Thirty-two male Wistar rats were randomly divided into four groups: G-S (sham: n = 8), G-NAC (NAC: n = 8), G-HBO (HBO: n = 8), and G-HN (HBO plus NAC: n = 8). A rectangular skin flap (2 × 8 cm 2 ) was dissected from the muscular dorsal layer, preserving the cranial pedicle. Polyethylene film was placed over the muscular layer and an interrupted 3.0 nylon suture was employed to fix the flap into the original place. On the eighth day, full-thickness biopsy samples (2 × 1 cm 2 ) were collected from the proximal, middle, and cranial areas of the skin flap, and in a site away from the flap labelled as the control area. Results: The measurements of necrotic areas in the groups were 18.3% in G-S, 24.3% in G-NAC, 12.6% in G-HBO, and 14.9% in G-HN. Significant difference was observed between the groups G-HBO and G-HN as well as G-NAC. Conclusion: HBO is associated with reduced area of necrosis of skin flap. The G-NAC group was associated with poor results when examined in isolation. The association between HBO and NAC did not produce favourable results with respect to the use of HBO alone. These findings suggest that the diffusion of oxygen through the interstitial space was the determining factor of more favourable results of HBO.

scholarly journals Effects of platelet-rich plasma gel on skin healing in surgical wound in horses

2009 ◽  
Vol 24 (4) ◽  
pp. 276-281 ◽  
Author(s):  
Rafael DeRossi ◽  
Anna Carolina Anciliero de Oliveira Coelho ◽  
Gisele Silveira de Mello ◽  
Fabrício Oliveira Frazílio ◽  
Cássia Rejane Brito Leal ◽  
...  
Keyword(s):  
Wound Repair ◽  
Platelet Rich Plasma ◽  
Low Cost ◽  
Differentiation Markers ◽  
Surgical Wounds ◽  
Surgical Incisions ◽  
Dermal Collagen ◽  
Skin Healing ◽  
Prp Gel ◽  
Made In

PURPOSE: To establish a low-cost method to prepare platelet-rich plasma (PRP) and evaluates the potential of platelet derived factors to enhance wound healing in the surgical wounds in equine. METHODS: To obtain a PRP gel, calcium gluconate and autologous thrombin were added to platelet-rich plasma. For the tests six saddle horses were used and two surgical incisions were made in each animal. Wounds were treated with PRP gel or untreated. Sequential wound biopsies collected at Treatment 1: at days 5 and 30 and Treatment 2: at days 15 and 45 post wounding permitted comparison of differentiation markers and wound repair. RESULTS: The optimal platelets enrichment over 4.0 time's baseline values was obtained using 300 g for 10 min on the first centrifugation and 640 g for 10 min on the second centrifugation. CONCLUSION: Wounds treated with PRP gel exhibit more rapid epithelial differentiation and enhanced organization of dermal collagen compared to controls in equine

The Role of Photoplethysmography in the Prediction of the Experimental Flap Survival

1978 ◽  
Vol 86 (3) ◽  
pp. ORL-492-ORL-497 ◽  
Author(s):  
Janusz Bardach ◽  
Richard J. Voots ◽  
Brian F. McCabe ◽  
Mow Ming Hsu
Keyword(s):  
Skin Flap ◽  
Skin Flaps ◽  
Arterial Blood ◽  
Flap Viability ◽  
Significant Difference ◽  
Blood Density ◽  
Density Scale ◽  
Experimental Flap ◽  
Venous Vasculature

A standard photoplethysmograph, modified to differentiate between arterial and venous vasculature in skin tissue, has been used to measure arterial supply in 100 unipedicle skin flaps in 25 pigs. A datum transform from a light intensity scale to a relative arterial blood density scale has been introduced and empirically labeled the vascular coefficient (VC). Statistical analysis of the VC data shows a significant difference between necrotic and surviving flap group mean data as early as 24 hours. Additional analysis in which a normal ogive was fitted to the sample VC data distributions suggests that statistically significant predictions of the probability of eventual flap necrosis can be made using VC measurements obtained immediately postoperatively. It is concluded that the modified photoplethysmograph, in conjunction with the VC data transform, constitutes a usable measuring technique for skin flap viability prediction in the experimental animal.

scholarly journals Effect of Hevea brasiliensis latex sap gel on healing of acute skin wounds induced on the back of rats

2016 ◽  
Vol 43 (1) ◽  
pp. 48-53 ◽  
Author(s):  
Maria Vitória Carmo Penhavel ◽  
Victor Henrique Tavares ◽  
Fabiana Pirani Carneiro ◽  
João Batista de Sousa
Keyword(s):  
Wound Closure ◽  
Skin Flap ◽  
Muscle Layer ◽  
Control Group ◽  
Histopathological Study ◽  
Dorsal Skin ◽  
Experimental Conditions ◽  
Cutaneous Wounds ◽  
Study Results ◽  
Significant Difference

Objective : to evaluate the effect of topical delivery of latex cream-gel in acute cutaneous wounds induced on the back of rats. Methods : we subjected sixteen rats to dermo-epidermal excision of a round dorsal skin flap, with 2.5cm diameter. We divided the animals into two groups: Latex Group: application of cream-gel-based latex throughout the wound bed on postoperative days zero, three, six and nine; Control group: no treatment on the wound. Photographs of the lesions were taken on the procedure day and on the 6th and 14th postoperative days, for analyzing the area and the larger diameter of the wound. We carried out euthanasia of all animals on the 14th postoperative day, when we resected he dorsal skin and the underlying muscle layer supporting the wound for histopathological study. Results : there was no statistically significant difference in the percentage of wound closure, in the histopathological findings or in the reduction of the area and of the largest diameter of the wounds among the groups studied on the 14th postoperative day. Conclusion : according to the experimental conditions in which the study was conducted, latex cream-gel did not interfere in the healing of acute cutaneous wounds in rats.

scholarly journals A possible Injectable Tissue Engineered Nucleus Pulposus constructed with platelet-rich plasma and ADSCs in Vitro

2020 ◽  
Author(s):  
Zhicheng Zhang ◽  
Jian Ma ◽  
Dajiang Ren ◽  
Fang Li
Keyword(s):  
Nucleus Pulposus ◽  
Platelet Rich Plasma ◽  
Positive Staining ◽  
Adipose Derived Stromal Cells ◽  
Genes Expression ◽  
Significant Difference ◽  
Safranin O ◽  
Collagen Genes ◽  
Prp Gel

Abstract Background: Injectable tissue engineered nucleus pulposus is a new idea for minimally invasive repair for degenerative intervertebral disc. The platelet-rich plasma (PRP) and adipose derived stromal cells(ADSCs) could be harvested from autologous tissue easily. PRP has mixed autologous growth factors and fibrous reticulate structure which may have the potential to make ADSCs differentiated into nucleus pulposus-like cells. The goal of this study was to explore the feasibility of constructing a possible injectable tissue engineered nucleus pulposus with PRP gel scaffold and ADSCs. Methods: After the rabbit ADSCs were identified with flow cytometry, the ADSCs were seeded into PRP gel and cultured in vitro. At the 2nd, 4th and 8th week, the PRP gel/ADSCs complex was observed by macroscopy, histological staining, BrdU immunofluorescence, and scanning electron microscopy. The glycosaminoglycans (GAG) in the PRP gel/ADSCs complex were measured by safranin O staining with spectrophotometry. PRP gel/ADSCs complex genes expression of HIF-1α, Aggrecan, Type Ⅱ collagen were detected by RT-PCR. The injectability of this complex was tested. Results: Macroscopically, the complex was solidified into gel with smooth surface and good elasticity. The safranin O staining confirmed almost no positive staining at 2nd week, however, the positive staining of extracellular matrix was enhanced obviously at 4th, 8th week. The HE staining and SEM demonstrated that the cells were well-distributed in the reticulate scaffold. BrdU immunofluorescence showed that ADSCs can survive and proliferate in PRP gel at each time points. The level of GAG at 4th week was higher than those at 2nd week (P<0.05), and significant difference was also noted between 4th and 8th week (P<0.05). HIF-1α, Aggrecan, Type Ⅱ collagen genes expression at 4th week were much more than those at 2nd week (P<0.05), and significant differences were also noted between 4th and 8th week (P<0.05). The flow rate of complex was 0.287 mL/min when passed through the 19-gauge needle with the 100mmHg pressure. Conclusions: The PRP gel made it possible for rabbit ADSCs differentiated into nucleus pulposus-like cells after mixed culture in vitro. Maybe, it is a better feasible method for construction of autologous injectable tissue engineered nucleus pulposus.

scholarly journals Profil Darah Pada Kucing Selama Proses Kesembuhan Luka Melalui Teknik Skin Flaps Rotasi Yang Dirawat Dengan Dry Dressing Dan Moist Dressing

2020 ◽  
Vol 10 (1) ◽  
pp. 37
Author(s):  
Erwin Erwin ◽  
Rusli Rusli ◽  
Fidella Diva Jones
Keyword(s):  
Skin Flap ◽  
Healing Process ◽  
Treatment Method ◽  
Wound Healing Process ◽  
Skin Flaps ◽  
Blood Profile ◽  
Treatment Groups ◽  
Significant Difference ◽  
Group 2 ◽  
Method Group

The skin flap is one of the techniques employed to reconstruct defects and close wounds. This study aims to observe the blood profile in cats during the healing process of rotation skin flaps that were treated with a dry and moist dressing. This study used six male cats within the age of 1-2 years old and weighed among 2-4 kg, divided into two treatment groups. All of the specimens were treated with the following procedure; the fur on the abdominal area was shaved and disinfectant was applied to the area between the os sacrum and os femur, lastly, 2 cm2 triangle wound was deliberately made on the area. The treatment of the wound was done by the rotation skin flaps method. Group 1 was treated with sterile bandage and group 2 was treated with sofratulle®. Blood samples were taken from the cephalica antebrachii vein on the 1st, 3rd, 6th, and 12th days. The erythrocytes, hemoglobin, hematocrit, platelets, leukocytes, neutrophils, basophils, eosinophils, monocytes, and lymphocytes were counted by hematology analyzer. The results showed that there is a significant difference (P<0.05) in the increase of neutrophil levels between the group treated with sterile kassa (P-1) and the group treated with sofratulle® (P-2). The number of the cats’ red blood cells rose on the 3rd day and rose again on the 12th day after the treatment with a significant difference (P<0.05) during the observation. In conclusion, the rotation flaps treatment method with dry dressing and moist dressing in the wound healing process affected the cats’ blood profile and the moist dressing was preferred to the dry dressing. 

scholarly journals Platelet-Rich Plasma, Hydroxyapatite, and Chitosan in the Bone and Cartilaginous Regeneration of Femoral Trochlea in Rabbits: Clinical, Radiographic, and Histomorphometric Evaluations

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Francisco Alipio de Sousa Segundo ◽  
Edla Iris de Sousa Costa ◽  
Adílio Santos de Azevedo ◽  
Ana Lucélia de Araújo ◽  
Ana Clara de França Silva ◽  
...  
Keyword(s):  
Subchondral Bone ◽  
Platelet Rich Plasma ◽  
Radiographic Evaluation ◽  
Femoral Trochlea ◽  
Microscopic Evaluation ◽  
Significant Difference ◽  
Bone Reaction ◽  
Microscopic Findings ◽  
And Cartilage ◽  
Made In

The aim of this study was to evaluate the trochlear bone and cartilaginous regeneration of rabbits using the association of PRP, chitosan, and hydroxyapatite. Hole was made in rabbit troches, one hole in each animal remained empty (group C), and one was filled by a combination of PRP, chitosan, and hydroxyapatite (group T). Clinical-orthopedic, radiographic, and histomorphometric evaluations were performed. Clinical-orthopedic evaluation showed lameness of two members of the T group and one member of group C. The radiographic evaluation showed that the T group showed absence of subchondral bone reaction (33%). The presence of moderate subchondral bone reaction was more frequently reported in group C with 67%. Microscopic evaluation revealed a presence of tissue neoformation, composed of connective tissue. Microscopic findings were similar in both groups, with a difference in the amount of neoformed tissue being perceptible, which was confirmed after the morphometric analysis, which revealed a significant difference in the quantity of newly formed tissue at the bone/cartilage/implant interface. The composite base of the association of chitosan, hydroxyapatite, and platelet-rich plasma favored bone and cartilage healing.

scholarly journals Prophylactic Mastectomy: Postoperative Skin Flap Thickness Evaluated by MRT, Ultrasound and Clinical Examination

2020 ◽  
Vol 27 (7) ◽  
pp. 2221-2228
Author(s):  
Rebecca Wiberg ◽  
Magnus N. Andersson ◽  
Johan Svensson ◽  
Anna Rosén ◽  
Freja Koch ◽  
...  
Keyword(s):  
Clinical Examination ◽  
Prophylactic Mastectomy ◽  
Skin Flap ◽  
Magnetic Resonance Tomography ◽  
Plastic Surgeon ◽  
General Surgeon ◽  
Skin Flaps ◽  
Significant Difference ◽  
Flap Thickness ◽  
The Mean

Abstract Background Women with an increased hereditary risk of breast cancer can undergo prophylactic mastectomy (PM), which provides a significant, but not total, risk reduction. There is an ongoing discussion about how much skin and subcutaneous tissue should be resected to perform an adequate PM while leaving viable skin flaps. Methods Forty-five women who had undergone PM were examined with magnetic resonance tomography (MRT), ultrasound (US) and clinical examination (CE) by a plastic surgeon and a general surgeon to estimate skin flap thickness. Results The estimated mean skin flap thickness after PM was 13.3 (± 9.6), 7.0 (± 3.3), 6.9 (± 2.8) and 7.4 (± 2.8) mm following MRT, US, and CE performed by a plastic surgeon and a general surgeon, respectively. The mean difference in estimated skin flap thickness was significant between MRT and the other measuring methods, while there was no significant difference between US and CE, nor between CE performed by the surgeons. The mean skin flap thickness was significantly affected by the age at PM. Following PM, necrosis was detected in 7/23 (30.4%) of the breasts in skin flaps ≤ 5 mm and in 5/46 (10.9%) of the breasts in skin flaps > 5 mm (OR 6.29; CI 1.20–32.94; p = 0.03). Conclusion The odds of getting postoperative necrosis was > 6 times higher in skin flaps ≤ 5 mm. Thus, if the degree of remaining glandular tissue is acceptably low, it is desirable to create skin flaps thicker than 5 mm to prevent wound healing problems after the PM procedure.

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