scholarly journals Acute toxicity of a deltamethrin based pesticide (DBP) to the Neotropical electric fish Microsternarchus cf. bilineatus (Gymnotiformes)

2020 ◽  
Vol 50 (4) ◽  
pp. 355-362
Author(s):  
Valesca de Souza CHAVES ◽  
Jaydione Luiz MARCON ◽  
Wallice Paxiúba DUNCAN ◽  
José Antônio ALVES-GOMES
Keyword(s):  
Acute Toxicity ◽  
Aquatic Ecosystems ◽  
Nervous Tissue ◽  
Ache Activity ◽  
Electric Fish ◽  
Exposure Period ◽  
Glutathione S Transferase ◽  
Biochemical Biomarkers ◽  
Amazonian Fish ◽  
Gst Activity

ABSTRACT Deltamethrin is a pyrethroid insecticide widely used in pest control in Brazilian agriculture. The intensive and disordered use of this pesticide in the Amazon region can drive it into aquatic ecosystems in several ways, but mainly by runoff and leaching. The present study was conceived to determine the acute toxicity (LC50) of a deltamethrin based pesticide (DBP) and to characterize its effects on two biochemical biomarkers, glutathione-S-transferase (GST) and acetylcholinesterase (AChE), in tissues of the electric fish Microsternarchus cf. bilineatus. Fishes were exposed to concentrations of 1, 2, 3, 4 and 5 μg L-1 of DBP for up to 96 hours. For each treatment, the absolute activity of the enzymes GST (muscle and liver) and AChE (muscle and nervous tissue) were analyzed. The LC50-96 h for Microsternarchus cf. bilineatus was 2.15 μg L-1, the lowest concentration registered for an Amazonian fish species so far. None of the concentrations tested of this insecticide affected AChE activity for the exposure period tested. A significant increase in muscle GST activity was detected only for concentrations of 2 and 3 μg L-1.

scholarly journals Different Enzymatic Activities in Carp (Cyprinus Carpio L.) as Potential Biomarkers of Exposure to the Pesticide Methomyl

2014 ◽  
Vol 65 (3) ◽  
pp. 311-318 ◽  
Author(s):  
David Hernández-Moreno ◽  
Irene de la Casa-Resino ◽  
José Maria Flores ◽  
Manuel José González-Gómez ◽  
Carlos María Neila ◽  
...  
Keyword(s):  
Ache Activity ◽  
Enzymatic Activities ◽  
Glutathione S Transferase ◽  
Mean Values ◽  
Exposed Fish ◽  
Biomarkers Of Exposure ◽  
Brain Ache Activity ◽  
And Control ◽  
Gst Activity ◽  
Biomarkers Of Effect

AbstractThis study investigated the influence of the pesticide methomyl on different enzymatic activities in carp. The fish were exposed to a sub-lethal concentration (0.34 mg L-1) of methomyl for 15 days. On days 4 and 15, catalase (CAT) and glutathione-S-transferase (GST) activities were measured in the liver and gills. Acetylcholinesterase (AChE) activity in brain and muscle was also determined. Liver catalase activity slightly increased in exposed fish when compared to controls, but it was statistically significant only at the beginning of the experiment. No changes in CAT activity in the gills of exposed and control animals were observed (mean values were in the range 10.7-11.7 nmol min-1 per mg of protein). Liver GST activity was slightly inhibited in the exposed animals at the beginning of the study; however, it was significantly inhibited in the gills. Brain AChE activity was diminished throughout the experiment and significantly decreased after 96 h of exposure compared to controls (0.041 vs. 0.075 nmol min1 per mg of protein; p<0.001). Our findings suggest that CAT, GST, and AChE are reliable biomarkers of effect after exposure to methomyl.

scholarly journals Activity of biochemical biomarkers in grasshoppers Abracris flavolineata (De Geer, 1773) (Orthoptera: Acrididae: Ommatolampidinae)

2021 ◽  
Vol 10 (11) ◽  
pp. e409101119877
Author(s):  
Ana Catia Santos da Silva ◽  
Aline Santos dos Santos ◽  
Theila dos Santos Santana ◽  
Elissandra Ulbricht Winkaler ◽  
Marcos Gonçalves Lhano
Keyword(s):  
Sampling Site ◽  
Sampling Effort ◽  
Glutathione S Transferase ◽  
Lateral Region ◽  
Biochemical Biomarkers ◽  
Forest Remnant ◽  
Males And Females ◽  
Gst Activity ◽  
Sweep Net ◽  
Made In

Biochemical biomarkers are commonly used in environmental monitoring programs due to their sensitivity to certain pollutants. From this perspective, their responses can be used as indicators of environmental quality. The present study aimed to determine the activity of the catalase (CAT) and glutathione-S-transferase (GST) enzymes in grasshoppers Abracris flavolineata (De Geer, 1773) from two forest remnant areas in Serra da Jiboia (BA) and compare them between males and females. The specimens were collected at two sites in Serra da Jiboia (Bahia, Brasil), named ‘Baixa de Areia’ and ‘Baixa Grande’. The animals were actively collected in the morning using a sweep net and a 2.5 h sampling effort. In total, 160 individuals were collected, with 80 individuals from each sampling site, 50 males and 30 females. After identification, an incision was made in the lateral region of the abdomen to remove the midgut, which was used to extract the CAT and GST enzymes. The results obtained demonstrated that CAT and GST activity did not vary significantly between sampling areas. However, with regard to sex, enzyme activity was significantly higher in males (p<0.005) in both locations. This is a pioneer study on the responses of CAT and GST activity in grasshoppers in Brazil.

Effects of CGA-154281 and Temperature on Metolachlor Absorption and Metabolism, Glutathione Content, and Glutathione-S-Transferase Activity in Corn (Zea mays)

Weed Science ◽  
1991 ◽  
Vol 39 (3) ◽  
pp. 324-328 ◽  
Author(s):  
Paul R. Viger ◽  
Charlotte V. Eberlein ◽  
E. Patrick Fuerst ◽  
John W. Gronwald
Keyword(s):  
Temperature Regime ◽  
Temperature Effects ◽  
Exposure Period ◽  
Transferase Activity ◽  
Cool Temperature ◽  
Warm Temperature ◽  
Glutathione S Transferase ◽  
Untreated Soil ◽  
Metabolism Rate ◽  
Gst Activity

CGA-154281 and temperature effects on metolachlor absorption and metabolism were studied in corn seedlings grown in untreated soil or soil treated with metolachlor, CGA-154281, or both. Seedlings were grown under a cool (21/13 C, 16-h day) or warm (30/21 C, 16-h day) temperature regime and exposed to14C-metolachlor for 10 min at either 21 or 30 C. Corn grown under the cool temperature regime absorbed slightly more14C-metolachlor than corn grown under the warm temperature regime. Corn held at 21 C during a 10-min14C-metolachlor exposure period metabolized metolachlor more slowly than corn held at 30 C. Decreased corn tolerance to metolachlor observed at lower temperatures may be due in part to slower metolachlor metabolism. Corn grown in the presence of metolachlor plus CGA-154281 metabolized14C-metolachlor to the glutathione conjugate twice as fast as corn grown in the presence of metolachlor alone. The increase in metabolism rate was due to a fivefold increase in glutathione-S-transferase (GST, EC 2.5.1.18) activity and not to an increase in glutathione (GSH) content. Results are consistent with the hypothesis that CGA-154281 protects corn from metolachlor injury by enhancing GST activity, which accelerates metolachlor detoxification via GSH conjugation.

Chemoprotective Effects of Propolis on Aflatoxin B1-Induced Hepatotoxicity in Rats: Oxidative Damage and Hepatotoxicity by Modulating TP53, Oxidative Stress

2020 ◽  
Vol 17 (3) ◽  
pp. 191-199
Author(s):  
Seval Yilmaz ◽  
Fatih Mehmet Kandemir ◽  
Emre Kaya ◽  
Mustafa Ozkaraca
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Oxidative Damage ◽  
Aflatoxin B1 ◽  
Tp53 Gene ◽  
Control Group ◽  
Glutathione S Transferase ◽  
Gene Expressions ◽  
Cat Gene ◽  
Gst Activity

Objective: This study aimed to detect hepatic oxidative damage caused by aflatoxin B1 (AFB1), as well as to examine how propolis protects against hepatotoxic effects of AFB1. Method: Rats were split into four groups as control group, AFB1 group, propolis group, AFB1+ propolis group. Results: There was significant increase in malondialdehyde (MDA) level and tumor suppressor protein (TP53) gene expression, Glutathione (GSH) level, Catalase (CAT) activity, CAT gene expression decreased in AFB1 group in blood. MDA level and Glutathione-S-Transferase (GST) activity, GST and TP53 gene expressions increased in AFB1 group, whereas GSH level and CAT activity alongside CAT gene expression decreased in liver. AFB1+propolis group showed significant decrease in MDA level, GST activity, TP53 and GST gene expressions, GSH level and CAT activity and CAT gene expression increased in liver compared to AFB1 group. Conclusion: These results suggest that propolis may potentially be natural agent that prevents AFB1- induced oxidative stress and hepatotoxicity.

Improved herbicide selectivity in tomato by safening action of benoxacor and fenclorim

2020 ◽  
Vol 34 (5) ◽  
pp. 647-651
Author(s):  
Edicarlos Castro ◽  
Carolina Pucci ◽  
Stefano Duarte ◽  
Nilda Roma Burgos ◽  
Te Ming Tseng
Keyword(s):  
Seed Treatment ◽  
Mechanisms Of Action ◽  
Shoot Biomass ◽  
Glutathione S Transferase ◽  
Visible Injury ◽  
Factor B ◽  
Tomato Plants ◽  
Alternative Weed Control ◽  
Biomass Reduction ◽  
Gst Activity

AbstractSafeners have been widely used to reduce phytotoxicity to crops, thus serving as an alternative weed control strategy. Benoxacor and fenclorim safeners have the potential to protect plants from herbicide phytotoxicity by increasing glutathione S-transferase (GST) activity within the plant. The study aimed to evaluate the safening effect of benoxacor and fenclorim on tomato against selected herbicides applied POST. The experiment was conducted in a greenhouse in a completely randomized designed with four replications in a 9 × 3 factorial scheme, where Factor A consisted of eight herbicides including a nontreated control, and Factor B consisted of two safeners including a nontreated control. The herbicide treatments were sulfentrazone (0.220 kg ai ha−1), fomesafen (0.280 kg ai ha−1), flumioxazin (0.070 kg ai ha−1), linuron (1.200 kg ai ha−1), metribuzin (0.840 kg ai ha−1), pyroxasulfone (0.220 kg ai ha−1), and bicyclopyrone (0.040 kg ai ha−1). Safener treatments consisted of benoxacor (0.67 g L−1) and fenclorim (10 µM). Tomato seeds were immersed in safener solution before sowing and herbicides were applied when tomato plants were at the 3-leaf stage, or 25 days after sowing. Visible injury was scored at 3, 7, 14, and 21 d after application (DAA), and shoot biomass was recorded 21 DAA. Seed treatment with fenclorim reduced injury caused by imazamox and bicyclopyrone by 5.5 and 1.3 times, respectively, whereas benoxacor reduced the injury from bicyclopyrone 1.3 times. In addition, tomato plants pretreated with fenclorim showed a lesser reduction in biomass after application of imazamox, fomesafen, and metribuzin, whereas plants pretreated with benoxacor showed lesser biomass reduction after metribuzin application. Thus, the use of safeners promotes greater crop selectivity, allowing the application of herbicides with different mechanisms of action on the crop.

The Function of Chloroplast GST of Puccinellia Tenuiflora Seedling Leaves in Resistance to Na2CO3 Stress

2011 ◽  
Vol 343-344 ◽  
pp. 712-720 ◽  
Author(s):  
Guo Rong Sun ◽  
Xue Li Wu ◽  
Gang Chen ◽  
Jian Bo Wang ◽  
Wen Zhong Cao ◽  
...  
Keyword(s):  
Osmotic Potential ◽  
Culture Solution ◽  
Nonlinear Relationship ◽  
Stress Range ◽  
Glutathione S Transferase ◽  
Electric Conductance ◽  
Low Intensity ◽  
Puccinellia Tenuiflora ◽  
Change Tendency ◽  
Gst Activity

In order to probe into orderliness changes of Glutathione S-Transferase (GST) of chloroplast ofPuccinellia tenuifloraseedlings under Na2CO3stress and its function in resistance to Na2CO3stress, relative electric conductance, GST activity and the O2-.produce rate of the chloroplast, and the osmotic potential of leaves, and the osmotic potential of culture solution ofP. tenuifloraseedlings under different Na2CO3stress were concerned. The result shows that in the Na2CO3stress range of 0～0.4%, along with the increase of its intensity under different Na2CO3stress intensity, GST activity of the chloroplast of seedling leaves ofP. tenuiflorais strengthened and GST activity is rapidly weakened with the increase of the intensity of Na2CO3stress more than 0.4%. The change of GST activity of the chloroplast along with the osmotic potential of culture solution and seedling leaves, relative electric conductance of the seedling leaves as well as the O2-.produce rate have the similar change tendency. There is significant nonlinear relationship among GST activity of chloroplast, osmotic potential of the seedling leaves and Na2CO3concentration ofculture solution, and among GST activity of chloroplast, O2-.produce rate and osmotic potential of seedling leaves, and among GST activity of chloroplast, osmotic potential of culture solution and that of seedling leaves, and among GST activity, the O2-.produce rate of chloroplast and relative electric conductance of the seedling leaves. These indicate that GST of chloroplast plays an important role in the process of seedlings ofP. tenuiflorain resistance to the low intensity of Na2CO3stress.

In vivo protection studies of bis-quaternary 2-(hydroxyimino)-N-(pyridin-3-yl) acetamide derivatives (HNK oximes) against tabun and soman poisoning in Swiss albino mice

2017 ◽  
Vol 36 (12) ◽  
pp. 1270-1285 ◽  
Author(s):  
P Kumar ◽  
D Swami ◽  
DP Nagar ◽  
KP Singh ◽  
J Acharya ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Ache Activity ◽  
Lethal Dose ◽  
Acute Poisoning ◽  
Control Group ◽  
Swiss Albino Mice ◽  
Albino Mice ◽  
Brain Ache Activity ◽  
Protection Index

The study reports antidotal efficacy of three HNK [ bis quaternary 2-(hydroxyimino)-N-(pyridin-3yl) acetamide derivatives] and pralidoxime (2-PAM), against soman and tabun poisoning in Swiss albino mice. Protection index (PI) was determined (treatment doses: HNK oximes, ×0.20 of their median lethal dose (LD50) and 2-PAM, 30 mg/kg, intramuscularly (im)) together with atropine (10 mg/kg, intraperitoneally). Probit log doses with difference of 0.301 log of LD50 of the nerve agents administered and inhibition of acetylcholinesterase (AChE) activity by 50% (IC50) was calculated at optimized time in brain and serum. Using various doses of tabun and soman (subcutaneously (sc)), in multiples of their IC50, AChE reactivation ability of the oximes was studied. Besides, acute toxicity (0.8× LD50, im, 24 h postexposure) of HNK-102 and 2-PAM was also compared by determining biochemical, hematological variables and making histopathological observations. Protection offered by HNK-102 against tabun poisoning was found to be four times higher compared to 2-PAM. However, nearly equal protection was noted with all the four oximes against soman poisoning. HNK-102 reactivated brain AChE activity by 1.5 times more than 2-PAM at IC50 dose of soman and tabun. Acute toxicity studies of HNK-102 and 2-PAM showed sporadic changes in urea, uric acid, aspartate aminotransferase, and so on compared to control group, however, not supported by histopathological investigations. The present investigation showed superiority of newly synthesized HNK-102 oxime over standard 2-PAM, as a better antidote, against acute poisoning of tabun (4.00 times) and soman (1.04 times), in Swiss albino mice.

Oesophagostomum dentatum: Effect of glutathione S-transferase (GST) inhibitors on GST activity and larval development

2011 ◽  
Vol 127 (4) ◽  
pp. 762-767 ◽  
Author(s):  
Anja Joachim ◽  
Esther Lautscham ◽  
Jana Christoffers ◽  
Bärbel Ruttkowski
Keyword(s):  
Larval Development ◽  
Glutathione S Transferase ◽  
Oesophagostomum Dentatum ◽  
Gst Activity
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