scholarly journals Effects of fluoride and Aloe vera tooh gel in artificial white spot lesions in vitro

2016 ◽  
Vol 64 (1) ◽  
pp. 56-61 ◽  
Author(s):  
Tânia Mara da SILVA ◽  
Beatriz Maria da FONSECA ◽  
Ana Luísa Leme Simões SALES ◽  
Priscila HOLLEBEN ◽  
Márcia Carneiro VALERA ◽  
...  
Keyword(s):  
Repeated Measures ◽  
Aloe Vera ◽  
White Spot ◽  
White Spot Lesion ◽  
White Spot Lesions ◽  
Repeated Measures Anova ◽  
Ph Cycling ◽  
Knoop Microhardness ◽  
Bovine Enamel

ABSTRACT Objective: The aim of this study was to evaluate the effects of toothbrushing using a fluoride toothpaste and Aloe vera tooth gel on artificial white spot lesions through the Knoop microhardness (KHN) analysis. Methods: Sound bovine enamel samples (2 mm/diameter and 2 mm/depth) were prepared and immersed in artificial white spot lesion for 24 h. The preparation of artificial white spot lesions was performed by pH-cycling process. The samples were randomly divided into two groups (n=20), according the dentifrice used: containing fluoride (Colgate Total 12) or Aloe vera (Forever Bright Aloe Vera Toothgel). The top surface of samples was submitted to 10,000, 25,000, 50,000 and 100,000 brushing cycles (200 g load) in an automatic brushing machine with abrasive slurry. The KHN analysis were evaluated at baseline, after immersion in artificial white spot lesion and after 10,000, 25,000, 50,000 and 100,000 cycles of brushing. Data were analyzed by two-way repeated measures ANOVA and Tukey tests (p=0.05). Results: The KHN values significantly increased after brushing cycles compared to demineralized means. No significant differences showed for dentifrice factor (p=0.263). However, there were statistically significant differences between groups in cycles of brushing times (p=0.0001). Conclusion: The toothpastes (containing fluoride or Aloe vera) were effective in increasing the superficial microhardness of artificial white spot lesions.

scholarly journals In vitro Caries-Preventive Effect of Fluoridated Orthodontic Resins against Cariogenic Challenge Stimulation

2012 ◽  
Vol 13 (4) ◽  
pp. 452-455 ◽  
Author(s):  
Bhushan Arun Jawale ◽  
Neeraj Patil ◽  
Rahul Redasani ◽  
Lalit Chaudhari ◽  
JB Garde ◽  
...  
Keyword(s):  
Artificial Saliva ◽  
White Spot ◽  
White Spot Lesion ◽  
White Spot Lesions ◽  
Preventive Effect ◽  
Bonding Agents ◽  
Ph Cycling ◽  
Preventive Methods ◽  
Caries Lesions

ABSTRACT Aim The aim of the present study was to evaluate the in vitro caries preventive effect of fluoridated orthodontic resins under pH cycling with two types of acid demineralizing saliva. Materials and methods Brackets were bonded to 120 extracted human premolars, using Rely-a-bond (n = 40), Tru- Bond (n = 40) and Ortho-one (n = 40) orthodontic bonding agents. Each group of resin was divided into 2 subgroups (n = 20): immersion in remineralizing artificial saliva for 14 days and acid saliva with pH 4.3. After 14 days of pH cycling the caries preventive effect on the development of white spot lesion was evaluated considering the presence of inhibition zones to white spot lesions using two scores: 0 = absence and 1 = presence. Kruskal-Wallis ANOVA and Mann-Whitney U tests were used. Results Formation of white spot lesions was observed only under pH cycling using acid saliva with pH 4.3; with Rely-a-bond and Tru-Bond being significantly more effective in preventing the appearance of white spot lesions effect than Ortho-one. Conclusion The acidity of the demineralizing solution influenced the formation of white spot lesions around orthodontic brackets under highly cariogenic conditions. Rely-a-bond and Tru-bond presented higher caries-preventive effect than Orthoone. Clinical significance The development of fluoride-containing materials cannot be regarded as a permanent means to control dental caries lesions, but a complement along with other preventive methods. How to cite this article Patil N, Jawale B, Redasani R, Chaudhari L, Garde JB, Chauhan VS. In vitro Caries-Preventive Effect of Fluoridated Orthodontic Resins against Cariogenic Challenge Stimulation. J Contemp Dent Pract 2012;13(4):452-455.

scholarly journals Preventive effect of fluoridated orthodontic resins subjected to high cariogenic challenges

2010 ◽  
Vol 21 (3) ◽  
pp. 211-215 ◽  
Author(s):  
Paula Passalini ◽  
Tatiana Kelly da Silva Fidalgo ◽  
Erika Machado Caldeira ◽  
Rogerio Gleiser ◽  
Matilde da Cunha Gonçalves Nojima ◽  
...  
Keyword(s):  
Color Change ◽  
Artificial Saliva ◽  
Orthodontic Brackets ◽  
White Spot ◽  
White Spot Lesion ◽  
White Spot Lesions ◽  
Preventive Effect ◽  
Ph Cycling ◽  
Inhibition Zones

The aim of the present study was to evaluate the in vitro caries preventive effect of fluoridated orthodontic resins under pH cycling with two types of acid demineralizing saliva. Brackets were bonded to 60 bovine incisors, using either Transbond Plus Color Change (n=30) or Orthodontic Fill Magic (n=30) orthodontic resins. Each group of resin was divided into 3 subgroups (n=10): immersion in remineralizing artificial saliva for 14 days, pH cycling with high cariogenic challenge in acid saliva with pH 5.5, and acid saliva with pH 4.5. After 14 days of pH cycling, the caries preventive effect on the development of white spot lesion was evaluated considering the presence of inhibition zones to white spot lesions using two scores: 0= absence and 1= presence. Kruskal Wallis and Mann-Whitney tests (a=0.05) were used. Formation of white spot lesions was observed only under pH cycling using acid saliva with pH 4.5; with Transbond Plus Color Change being significantly more effective (p<0.05) in preventing the appearance of white spot lesions effect than Orthodontic Fill Magic. The acidity of the demineralizing solution influenced the formation of white spot lesions around orthodontic brackets under highly cariogenic conditions. Transbond Plus Color Change resin presented higher caries preventive effect than Orthodontic Fill Magic.

scholarly journals Efficacy of fluoride varnishes with different compositions on white spot lesions remineralization

2021 ◽  
Vol 24 (3) ◽  
Author(s):  
Carlos Rocha Gomes Torres ◽  
Manuela Da Silva Spinola ◽  
Renata Falchete do Prado ◽  
Vinicius Aneas Rodrigues ◽  
Natalia Cortez Gutierrez ◽  
...  
Keyword(s):  
Artificial Saliva ◽  
White Spot ◽  
Initial Surface ◽  
White Spot Lesions ◽  
Test Results ◽  
Tukey Test ◽  
Ph Cycling ◽  
Knoop Microhardness ◽  
Bovine Enamel ◽  
One Way Anova

Objective: To evaluate the efficacy of different fluoride varnishes on white spot lesions (WSL) remineralization. Material and Methods: Polished bovine enamel specimens were obtained (n = 60) and had their initial surface Knoop microhardness (SMH) determined. WSL were created and the SMH was measured again. Then, specimens were allocated into six groups: C – Control (without varnish); BF – Bifluorid 12 (6% NaF + 6% CaF2); DP – Duraphat (5% NaF); PF – Profluorid (5% NaF); FP - Fluor Protector (0.2% NaF + 0.9% difluorsilane); CW - Clinpro White Varnish (5% NaF + 5% TCP). After varnishes application, specimens were immersed in artificial saliva for 24 h. Then, pH-cycling was performed for 8 days and SMH was measured. Data were analyzed by one-way ANOVA and Tukey’s test. Results: Non-significant differences were observed among the groups at baseline (p = 0.187) and after WSL formation (p = 0.999). After treatments, significant differences were observed among the groups (p = 0.001). Mean % of alteration (SD) and results of Tukey test were: C- 92.40 (12.10)a; PF- 88.66 (10.66)a; FP-  85.90 (14.49)ab; BF-  67.85 (17.86)bc; CW- 66.60 (18.48)c; DP- 58.62 (8.69)c. Conclusion:.  Bifluorid 12, Clinpro White Varnish, and Duraphat showed higher efficacy than artificial saliva in promoting the remineralization of WSL, nevertheless, none of the treatments were able to recover sound enamel baseline microhardness.   Keywords Dental caries; Fluoride; Fluoride varnishes.

scholarly journals Contact with Fluoride-Releasing Restorative Materials Can Arrest Simulated Approximal Caries Lesion

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Camila de Almeida Brandão Guglielmi ◽  
Ana Flávia Bissoto Calvo ◽  
Tamara Kerber Tedesco ◽  
Fausto Medeiros Mendes ◽  
Daniela Prócida Raggio
Keyword(s):  
Contact Point ◽  
White Spot ◽  
White Spot Lesion ◽  
White Spot Lesions ◽  
Double Blind ◽  
Restorative Materials ◽  
Ph Cycling ◽  
Approximal Surface ◽  
Mineral Loss

Previous studies have suggested that the presence of white-spot lesion is very probable when adjacent surface is affected by cavitated lesions. This study evaluated the potential of different fluoride-releasing restorative materials in arresting enamel white-spot lesions in approximal surface in contact with them,in vitro(I) andin situ(II). White-spot lesions were formed in 240 primary enamel specimens via pH-cycling. They were put in contact with cylindrical blocks of 6 materials (n=20): composite resin, 2 high-viscous glass ionomer cements (HVGIC), resin-modified GIC, resin-modified nanoionomer, and polyacid-modified resin. In both studies I and II, these settings were designed to simulate the contact point between the restoration and simulated approximal lesion. For study I, they were subjected to a new pH-cycling cariogenic challenge for 7 or 14 days (n=10). For study II, a randomized double-blindin situdesign was conducted in two phases (7/14 days) to promote cariogenic challenge. At the end of both studies, specimens were collected for mineral analysis by cross-sectional microhardness. Higher mineral loss was observed for lesions in contact with resin (p<0.001). HVGICs were the most efficient in preventing mineral loss, whereas other materials presented an intermediate behavior. It is concluded that fluoride-releasing materials can moderately reduce white-spot lesions progression, and HVGIC can arrest enamel lesion in approximal surface in contact with them.

scholarly journals Impact of Experimental Nano-HAP Pastes on Bovine Enamel and Dentin Submitted to a pH Cycling Model

2013 ◽  
Vol 24 (3) ◽  
pp. 273-278 ◽  
Author(s):  
Livia P. Comar ◽  
Beatriz M. Souza ◽  
Luiz Fernando Gracindo ◽  
Marilia A. R. Buzalaf ◽  
Ana Carolina Magalhaes
Keyword(s):  
Calcium Phosphate ◽  
Repeated Measures ◽  
Surface Hardness ◽  
In Vitro Study ◽  
Cross Sectional ◽  
Before And After ◽  
Mi Paste ◽  
Ph Cycling ◽  
Bovine Enamel

This in vitro study evaluated the preventive potential of experimental pastes containing 10% and 20% hydroxyapatite nanoparticles (Nano-HAP), with or without fluoride, on dental demineralization. Bovine enamel (n=15) and root dentin (n=15) specimens were divided into 9 groups according to their surface hardness: control (without treatment), 20 Nanop paste (20% HAP), 20 Nanop paste plus (20% HAP + 0.2% NaF), 10 Nanop paste (10% HAP), 10 Nanop paste plus (10% HAP + 0.2% NaF), placebo paste (without fluoride and HAP), fluoride paste (0.2% NaF), MI paste (CPP-ACP, casein phosphopeptide-amorphous calcium phosphate), and MI paste plus (CPP-ACP + 0.2% NaF). Both MI pastes were included as commercial control products containing calcium phosphate. The specimens were treated with the pastes twice a day (1 min), before and after demineralization. The specimens were subjected to a pH-cycling model (demineralization–6-8 h/ remineralization-16-18 h a day) for 7 days. The dental subsurface demineralization was analyzed using cross-sectional hardness (kgf/mm 2 , depth 10-220 µm). Data were tested using repeated-measures two-way ANOVA and Bonferroni's test (p<0.05). The only treatment able to reduce the loss of enamel and dentin subsurface hardness was fluoride paste (0.2% NaF), which differed significantly from the control at 30- and 50-µm depth (p<0.0001). The other treatments were not different from each other or compared with the control. The experimental Nanop pastes, regardless of the addition of fluoride, were unable to reduce dental demineralization in vitro.

scholarly journals Remineralization of enamel subsurface lesions using toothpaste containing tricalcium phosphate and fluoride: an in vitro µCT analysis

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Hidenori Hamba ◽  
Keiki Nakamura ◽  
Toru Nikaido ◽  
Junji Tagami ◽  
Takashi Muramatsu
Keyword(s):  
Tricalcium Phosphate ◽  
White Spot Lesions ◽  
Mineral Density ◽  
Small Crystals ◽  
Ph Cycling ◽  
X Ray Computed ◽  
Bovine Enamel ◽  
One Way Anova ◽  
Mineral Loss

Abstract Background This study aimed to compare the efficacies of experimental toothpastes containing functionalized tricalcium phosphate (fTCP) with and without fluoride for in vitro enamel remineralization under pH-cycling conditions. Methods To create artificial white spot lesions, 36 bovine enamel specimens were immersed in a demineralization solution for 10 days. During pH-cycling for 12 days, the specimens were divided into four groups based on the experimental toothpaste type used: (a) fTCP-free, fluoride-free (fTCP − F −); (b) fTCP-containing, fluoride-free (fTCP + F −); (c) fTCP-free, fluoride-containing (fTCP − F +); and (d) fTCP-containing, fluoride-containing (fTCP + F +). Micro-focus X-ray computed tomography (μCT) scans of all specimens were obtained before demineralization, after demineralization, and after pH-cycling. The mineral density and mineral loss (ΔZ) in the enamel subsurface lesions were measured and the percentage of remineralization (%R) was calculated from ΔZ after demineralization and pH-cycling. One-way ANOVA with Tukey’s test was used for statistical analysis of the %R values. The treated enamel surface was investigated via scanning electron microscopy (SEM). Results The fTCP − F − group presented with the lowest amount of mineral gain after pH-cycling. In contrast, the fTCP + F + group showed the highest degree of remineralization within all lesion parts. The %R was highest in the fTCP + F + group (38.2 ± 7.8, all P < 0.01). SEM revealed the presence of small crystals on the enamel rods in the fTCP + F − and fTCP + F + groups. Conclusions The experimental toothpaste containing fTCP and fluoride increased remineralization of the artificial enamel subsurface lesions during pH-cycling. Furthermore, fTCP and fluoride appear to act independently on the remineralization of enamel subsurface lesions, although they coexisted in one toothpaste type. Trial registration: This is not a human subject research.

Crush Absorbing Energy of White Spot Lesion Measured by Indentation Tests

2005 ◽  
Vol 898 ◽  
Author(s):  
Alison M. Fallgatter ◽  
Ching-Chang Ko ◽  
C-H Chou
Keyword(s):  
Mechanical Strength ◽  
Orthodontic Treatment ◽  
White Spot ◽  
White Spot Lesion ◽  
White Spot Lesions ◽  
Fluoride Treatment ◽  
Demineralized Enamel ◽  
Indentation Tests ◽  
Novel Method ◽  
Bovine Enamel

AbstractWhite spot lesions are clinically detectable areas of demineralized enamel that often form during orthodontic treatment. Fluoride has been shown to prevent demineralization from occurring. Mechanical properties of white spot lesions are not well characterized. Bovine enamel slabs, with and without fluoride treatment, were placed under demineralization conditions. Through a series of microindentations at incremental loads, mechanical strength was measured using a novel method, SVAE. Specific Volume Absorbing Energy (SVAE) is equal to work energy divided by the indentation volume. The supra-surface area (outmost 5 μm) of enamel slabs with fluoride demonstrated decreased mechanical strength compared to those without fluoride. Fluoride may not impart protection over long periods of demineralization.

scholarly journals Whitening mouthwash containing hydrogen peroxide decreases enamel microhardness in vitro.

2017 ◽  
Vol 16 ◽  
pp. 1-9 ◽  
Author(s):  
Fernando Pelegrim Fernandes ◽  
Cecília Pedroso Turssi ◽  
Fabiana Mantovani Gomes França ◽  
Roberta Tarkany Basting ◽  
Flávia Lucisano Botelho do Amaral
Keyword(s):  
Hydrogen Peroxide ◽  
Repeated Measures ◽  
Artificial Saliva ◽  
Control Group ◽  
Carbamide Peroxide ◽  
Knoop Microhardness ◽  
Bovine Enamel ◽  
Bleaching Treatment ◽  
Over Time

Aim: To assess the effect of a mouthwash containing hydrogen peroxide (HP) on Knoop microhardness (KMH) of bovine enamel. Methods: Fifty-one enamel slabs were polished and divided into groups (n=17), according to the product used during 28 days: HP – mouthwash containing 1,5% of HP (4 min, once/day); CP - 10% carbamide peroxide gel (2 hours/day); AS - no treatment (kept in artificial saliva (AS). Each fragment was submitted to KMH test (three indentations/fragment, with a 50 g load for 5 sec) four times: before (baseline); during (14 and 28 days) and after (7 days immerged in AS) the bleaching treatment. The data were submitted to repeated-measures two-way ANOVA (α=0.05). Results: There was no effect of the interaction between the time and treatment factors (p=0.327). No significant effect was observed from the time factor (p = 0.054). The factor treatment showed significant effect (p =0.002). Regardless of time, the KMH of the enamel submitted to HP was lower than the value observed with the use of CP, which did not differ significantly from the control group (AS). Conclusion: Although there was a trend of decreasing enamel microhardness over time, only the mouthwash containing hydrogen peroxide had a significant effect.

scholarly journals Control of White Spot Lesion Adjacent to Orthodontic Bracket with Use of Fluoride Varnish or Chlorhexidine Gel

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Manuel Restrepo ◽  
Diego G. Bussaneli ◽  
Fabiano Jeremias ◽  
Rita C. L. Cordeiro ◽  
Ana C. Magalhães ◽  
...  
Keyword(s):  
Repeated Measures ◽  
Mineral Content ◽  
Experimental Period ◽  
White Spot ◽  
Fluoride Varnish ◽  
White Spot Lesion ◽  
White Spot Lesions ◽  
Orthodontic Bracket ◽  
Before And After ◽  
Chlorhexidine Gel

The aims of this study were to compare the effectiveness of fluoride varnish and chlorhexidine gel in controlling white spot lesions (WSLs) adjacent to orthodontic brackets and to compare the ability of Quantitative Light-Induced Fluorescence (QLF) to measure mineral uptake with that of transverse microradiography (TMR). Thirty premolars with artificially induced WSLs were randomly assigned to three groups: (1) two applications of 5% NaF-varnish (F), with one-week interval, (2) two applications of 2% chlorhexidine gel (CHX), with one-week interval, and (3) control (CO), no treatment. QLF was used to measure changes in fluorescence before and after caries induction, 1 week after each application and 1, 2, and 3 months after the last application ofFor CHX. TMR was performed to quantify lesion depth and mineral content after caries induction to evaluate the effects ofF, CHX, and CO 3 months after the last application of agents. The data were analyzed by repeated measures ANOVA and Tukey’s test. All treatments increased the mineral content during the experimental period; however,Finduced faster remineralization than CHX. The correlation between QLF and TMR was significantly moderate. Two applications of fluoride varnish or 2% chlorhexidine gel at one-week intervals were effective in controlling WSLs.

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