Pollen viability and germination in Elaeis oleifera, Elaeis guineensis and their interspecific hybrid

Pesquisa Agropecuária Tropical ◽

10.1590/1983-40632021v5168076 ◽

2021 ◽

Vol 51 ◽

Author(s):

Deysi Jhoana Camayo Mosquera ◽

Daniel Gerardo Cayón Salinas ◽

Gustavo Adolfo Ligarreto Moreno

Keyword(s):

Interspecific Hybrid ◽

Pollen Germination ◽

Elaeis Guineensis ◽

Pollen Viability ◽

Pollen Grains ◽

Gene Pools ◽

Germination Test ◽

Elaeis Oleifera ◽

Sucrose Medium

ABSTRACT Elaeis oleifera chromosomes are similar to those of E. guineensis, with close gene pools for the production of interspecific O x G hybrids. The pollen viability and germination of E. oleifera ‘Coarí’ and E. guineensis ‘La Mé’ were compared to their interspecific hybrid O x G (‘Coarí’ x ‘La Mé’). The pollen viability was determined by the acetocarmine staining method (0.5 %) and the pollen germination by in vitro incubation on agar-sucrose medium (1.2-11.0 g in 100 mL of distilled water). The pollen viability and germination of the ‘Coarí’ x ‘La Mé’ hybrid were significantly lower than those of their parents. The percentage of pollen viability by acetocarmine staining was higher than that of in vitro germination, indicating that not all pollen grains classified as viable germinated on the agar-sucrose medium. The pollen germination test is a more reliable indicator than the staining viability test, because the latter only reveals that the pollen contains the enzymes necessary to initiate germination, while the germination test determines the emission and development of the pollen tube.

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In vitro pollen germination and viability of a Java × New Guinea Impatiens interspecific hybrid

Canadian Journal of Botany ◽

10.1139/b87-270 ◽

1987 ◽

Vol 65 (9) ◽

pp. 1967-1968 ◽

Cited By ~ 1

Author(s):

C. B. Hicks ◽

L. C. Stephens ◽

J. L. Weigle

Keyword(s):

Interspecific Hybrid ◽

Pollen Germination ◽

Agar Medium ◽

Pollen Viability ◽

Pollen Grains ◽

New Guinea ◽

Chi Square ◽

Pollen Diameter ◽

New Guinea Impatiens

Pollen germination on 2% agar medium was investigated as a method of screening pollen viability, using ISU 7851-1, a Java × New Guinea Impatiens interspecific hybrid. A chi-square test showed that percentage pollen germination was related to corresponding seed set. However, some pollinations yielded seeds from pollen that tested in vitro as low as 0.5% germination. Other Impatiens genotypes were screened rapidly for pollen viability by microscopically scanning for pollen germination in vitro. ISU 7851-1 pollen diameter sizes ranged from 18 to 81 μm on agar medium, but only pollen grains of 45 μm and larger were capable of in vitro germination.

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Effect of high temperature on the reproductive development of chickpea genotypes under controlled environments

Functional Plant Biology ◽

10.1071/fp12033 ◽

2012 ◽

Vol 39 (12) ◽

pp. 1009 ◽

Cited By ~ 72

Author(s):

Viola Devasirvatham ◽

Pooran M. Gaur ◽

Nalini Mallikarjuna ◽

Raju N. Tokachichu ◽

Richard M. Trethowan ◽

...

Keyword(s):

Heat Stress ◽

High Temperature ◽

Pollen Germination ◽

Pollen Viability ◽

Pollen Grains ◽

High Temperature Stress ◽

Pollen Production ◽

Seed Number ◽

Controlled Environments

High temperature during the reproductive stage in chickpea (Cicer arietinum L.) is a major cause of yield loss. The objective of this research was to determine whether that variation can be explained by differences in anther and pollen development under heat stress: the effect of high temperature during the pre- and post-anthesis periods on pollen viability, pollen germination in a medium, pollen germination on the stigma, pollen tube growth and pod set in a heat-tolerant (ICCV 92944) and a heat-sensitive (ICC 5912) genotype was studied. The plants were evaluated under heat stress and non-heat stress conditions in controlled environments. High temperature stress (29/16°C to 40/25°C) was gradually applied at flowering to study pollen viability and stigma receptivity including flower production, pod set and seed number. This was compared with a non-stress treatment (27/16°C). The high temperatures reduced pod set by reducing pollen viability and pollen production per flower. The ICCV 92944 pollen was viable at 35/20°C (41% fertile) and at 40/25°C (13% fertile), whereas ICC 5912 pollen was completely sterile at 35/20°C with no in vitro germination and no germination on the stigma. However, the stigma of ICC 5912 remained receptive at 35/20°C and non-stressed pollen (27/16°C) germinated on it during reciprocal crossing. These data indicate that pollen grains were more sensitive to high temperature than the stigma in chickpea. High temperature also reduced pollen production per flower, % pollen germination, pod set and seed number.

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Colchicine did not affect the viability of induced 2n pollen in Populus tomentosa

Silva Fennica ◽

10.14214/sf.10132 ◽

2019 ◽

Vol 53 (2) ◽

Cited By ~ 1

Author(s):

Yan Liu ◽

Yuan Zhang ◽

Qing Zhou ◽

Jian Wu ◽

Pingdong Zhang

Keyword(s):

Pollen Germination ◽

Pollen Viability ◽

Pollen Grains ◽

Populus Tomentosa ◽

2N Pollen ◽

Significant Difference ◽

Diploid Pollen ◽

Germination Experiments ◽

Diploid Gametes

Colchicine is widely used as a mutagen to induce production of diploid gametes in plants. However, whether colchicine affects induced pollen viability remains unclear. To clarify whether colchicine affected the viability of induced pollen, we induced production of diploid pollen by colchicine, followed by pollen germination and crossing induced pollen with normal gametes to produce triploid in CarriÃ¨re. The results showed that the predominant meiotic stages and the number of colchicine injections had significant effects on the occurrence rates of induced 2n pollen. When the colchicine injection was given at diakinesis, a significant decrease in the pollen production per bud was observed (â<â0.001). The morphology of the colchicine-induced 2n pollen was similar to that of the natural 2n pollen in its ectexine structure. The pollen germination experiments revealed that there was also no significant difference in germination rates between the induced diploid pollen and natural 2n pollen grains, and 68 triploids were created by crossing colchicine-induced pollen. Our findings revealed that colchicine injection could induce to produce 2n pollen and will not lead to dysfunction of induced diploid pollen.in vitroPopulus tomentosapP. tomentosa

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In vitro germination and viability of pea pollen grains after application of organic nano-fertilizers

Pesticidi i fitomedicina ◽

10.2298/pif1701061g ◽

2017 ◽

Vol 32 (1) ◽

pp. 61-65 ◽

Cited By ~ 1

Author(s):

Natalia Georgieva ◽

Ivelina Nikolova ◽

Valentin Kosev ◽

Yordanka Naydenova

Keyword(s):

Pollen Tube ◽

Pollen Germination ◽

Culture Media ◽

Pollen Viability ◽

Pollen Grains ◽

Pollen Grain ◽

In Vitro Germination ◽

Pisum Sativum L ◽

Pollen Tube Elongation

The objective of this study was to evaluate the influence of two organic nanofertilizers, Lithovit and Nagro, on in vitro germination, pollen tube elongation and pollen grain viability of Pisum sativum L cv. Pleven 4. The effect of their application was high and exceeded data for the untreated control (44.2 and 47.23 % regarding pollen germination and pollen tube elongation, respectively), as well as the effect of the control organic algal fertilizer Biofa (17.5 and 27.9 %, respectively). Pollen grains were inoculated in four culture media. A medium containing 15% sucrose and 1% agar had the most stimulating impact on pea pollen grains. Pollen viability, evaluated by staining with 1% carmine, was within limits of 74.72-87.97%. The highest viability of pollen grains was demonstrated after the application of Nagro organic nano-fertlizer.

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In vitro pollen germination and pollen viability in passion fruit (Passiflora spp.)

Revista Brasileira de Fruticultura ◽

10.1590/s0100-29452013000400023 ◽

2013 ◽

Vol 35 (4) ◽

pp. 1116-1126 ◽

Cited By ~ 7

Author(s):

Taliane Leila Soares ◽

Onildo Nunes de Jesus ◽

Janay Almeida dos Santos-Serejo ◽

Eder Jorge de Oliveira

Keyword(s):

Pollen Germination ◽

Culture Medium ◽

Pollen Viability ◽

Pollen Grains ◽

In Vitro Germination ◽

Triphenyltetrazolium Chloride ◽

Histochemical Analysis ◽

High Viability ◽

Three Stages

The use of Passiflora species for ornamental purposes has been recently developed, but little is known about pollen viability and the potential for crossing different species. The objective of this study was to evaluate the pollen viability of six Passiflora species collected from different physiological stages of development through in vitro germination and histochemical analysis using dyes. The pollen was collected in three stages (pre-anthesis, anthesis and post-anthesis). Three compositions of culture medium were used to evaluate the in vitro germination, and two dyes (2,3,5-triphenyltetrazolium chloride, or TTC, and Lugol's solution) were used for the histochemical analysis. The culture medium containing 0.03% Ca(NO3) 4H2O, 0.02% of Mg(SO4 ).7H2O, 0.01% of KNO3, 0,01% of H3BO3, 15% sucrose, and 0.8% agar, pH 7.0, showed a higher percentage of pollen grains germinated. Anthesis is the best time to collect pollen because it promotes high viability and germination. The Lugol's solution and TTC dye overestimated the viability of pollen, as all accessions showed high viability indices when compared with the results obtained in vitro.

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Improvised Media for In Vitro Pollen Germination of some Species of Apocynaceae

International Journal of Environment ◽

10.3126/ije.v3i3.11074 ◽

2014 ◽

Vol 3 (3) ◽

pp. 146-153

Author(s):

Reshmi Chatterjee ◽

Satadip Sarkar ◽

GM Narasimha Rao

Keyword(s):

Pollen Germination ◽

Germination Rate ◽

Pollen Viability ◽

Nitrate Solution ◽

Calcium Nitrate ◽

Pollen Grains ◽

In Vitro Pollen Germination ◽

Breeding Programmes ◽

Cultural Media

Pollen germination forms one of the most important stage post pollination prior to fertilization. This is essential for proper seed setting and seed development. In vitro pollen germination test is the most reliable way of assessing the pollen viability. In the present study pollen grains of seven genera under Apocynaceae family namely, Allamanda, Alstonia, Catharanthus, Nerium, Plumeria, Thevetia and Tabernaemontana were tested in some basic cultural media, such as Brewbaker’s media, 6% Glucose solution, 4% Calcium Nitrate solution and 3% Boron solution. Alstonia pollen grains exhibited highest percentage of germination rate in all the cultural media. Glucose and Brewbaker’s media is found to be highly suitable for efficient pollen germination in all the genera. Boron solution is effective for germination of pollen grains of tree species. In vitro pollen germination can be easily carried out in laboratories. These results can be utilised in plant breeding programmes to improve cultivar and varieties. DOI: http://dx.doi.org/10.3126/ije.v3i3.11074 International Journal of Environment Vol.3(3) 2014: 146-153

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Pollen tube growth of Paeonia tenuifolia L. (Paeoniaceae) in vitro and in vivo

Bangladesh Journal of Botany ◽

10.3329/bjb.v40i1.8003 ◽

1970 ◽

Vol 40 (1) ◽

pp. 93-95 ◽

Cited By ~ 1

Author(s):

Feruzan Dane ◽

Nuran Ekici

Keyword(s):

Pollen Tube ◽

Pollen Germination ◽

Pollen Viability ◽

Pollen Grains ◽

Pollen Tubes ◽

In Vivo Studies ◽

In Vivo Growth ◽

Paeonia Tenuifolia

In vitro and in vivo studies on pollen germination of Paeonia tenuifolia L. (Paeoniaceae) revealed that pollen grains are shed at two-celled stage. Normal and abnormal pollens were observed. Pollen viability was recorded between 55 and 75%. In vitro studies revealed 85% germination and usually the germination was monosphonic. Some pollen tubes with swollen tube tip and undulations were found. Styles and stigma were found to contain many pollen tubes 24 hrs after pollination. Key words: Paeonia tenuifolia; Pollen tube; In vitro growth; In vivo growth DOI: http://dx.doi.org/10.3329/bjb.v40i1.8003 Bangladesh J. Bot. 40(1): 93-95, 2011 (June)

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Pollination and pollen germination in common juniper (Juniperus communis: Cupressaceae)

Arctic Environmental Research ◽

10.3897/issn2541-8416.2018.18.4.162 ◽

2018 ◽

Vol 18 (4) ◽

pp. 162-174 ◽

Cited By ~ 1

Author(s):

Mikhail Surso

Keyword(s):

Pollen Germination ◽

Pollen Viability ◽

Chemical Properties ◽

Morphological Structure ◽

Pollen Grains ◽

Weather Conditions ◽

Pollen Tubes ◽

External Agents ◽

Amino Acids Composition

Pollination and fertilization are the most important and responsible stages in sexual reproduction of coniferous plants. The period of high concentrations of juniper pollen in overhead during pollination period is no more than 4–6 hours and within one calendar day. This dependence remains invariable from year to year, and does not depend neither from weather conditions, nor the peculiarities of the place where juniper grows. Influence of external agents on dynamics of exudation of a juniper pollination droplet is studied. The duration of exudation and volume of secretory liquid exuded by tissues of ovules during the period of prescription in the juniper depend from presence of external agents on a surface of pollination droplet, their sizes and physical and chemical properties. The pollination droplet chemical compound is studied. In sugars composition of juniper pollination droplet there are only two monosaccharides: glucose and galactose. the amino acids composition is prevailed by arginine, aspartic and glutamic acids. The results of the studies confirm complex chemical composition and multifunctionality of juniper pollination droplet. The pollination mechanism of juniper is effective and selective. The morphological structure of pollen grains of juniper predetermines the processes of pollen germination at early stages. The hydrophilic capsule, formed pollen hydration, promotes to exine rupture and shedding. This capsule remains until the fertilization. The distal tip of pollen tube remains in itduring all time of its growth. In culture in vitro development of pollen tubes of juniper proceeds non-uniformly. At definition of juniper pollen viability it is necessary to consider a stage of pollen tubes development.

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VEGETATIVE AND BUNCH COMPONENTS OF FIRST PSEUDOBACKCROSS OF Elaeis oleifera BRAZIL AND SURINAME ORIGIN IN NORTH SUMATRA, INDONESIA

Jurnal Penelitian Kelapa Sawit ◽

10.22302/iopri.jur.jpks.v26i1.13 ◽

2018 ◽

Vol 26 (1) ◽

pp. 23-28

Author(s):

Heri Adriwan Siregar ◽

Hernawan Yuli Rahmadi ◽

Retno Diah Setiowati ◽

Edy Suprianto

Keyword(s):

Interspecific Hybrid ◽

Plant Architecture ◽

Elaeis Guineensis ◽

Morphological Properties ◽

Height Increment ◽

Elaeis Oleifera ◽

Almost All ◽

North Sumatra ◽

Two Populations ◽

Hybrid Cross

An Attempt to combine the superior traits of Elaeis oleifera and Elaeis guineensis have been done through an interspecific hybrid cross and followed by pseudo-backcross 1 (pBC1). Observation of vegetative morphology and bunch components are presented in this paper. Two populations of pBC1 E. oleifera from the Suriname and Brazil origin were planted in 1990, 1993, 1995, and 2005, and were intensively observed for vegetative morphological properties and bunch components in November 2016 to February 2018. The results showed that almost all the individuals of pBC1 grew upright such as E. guineensis, no longer growing horizontally like the wild E. oleifera and the interspecific hybrid populations. The datas showed that the Suriname population plant architecture are compact or smaller than the Brazilian origin including the height increment and the size of the stem, the frond architecture and its components. Similarly, the bunch components show that the pBC1 Brazil is slightly superior to Suriname pBC1.

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