scholarly journals Treating sunflower seeds subjected to ozonization

2015 ◽  
Vol 37 (3) ◽  
pp. 202-210 ◽  
Author(s):  
Vitor Oliveira Rodrigues ◽  
Fabiano Ramos Costa ◽  
Marcela Carlota Nery ◽  
Sara Michelly Cruz ◽  
Soryana Gonçalves Ferreira de Melo ◽  
...  

Abstract:Sunflower crops (Helianthus annuus L.) are a source of pathogens that can be transmitted by means of the seeds. An alternative for the treatment of seeds can be the application of an ozone compound, which has been used in various segments to eliminate microorganisms. Three lots of the Embrapa 122 variety have been used to assess the efficacy of ozone to control pathogens in sunflowers seeds. To typify the profile of the lots we have determined the moisture content, the first germination counting, the germination, the germination speed index, emergence, initial stand, emergence speed index and health. The lots have been stored in an ozone-rich environment for periods of 20, 60 and 120 minutes and without ozone (control). After the treatments, the seeds were tested to assess their health and physiological quality through the said tests as well as a test for accelerating aging, tetrazolium and incidence and severity of fungi. It has been concluded that the treatment for H. annuus seeds, Embrapa 122 variety, with ozone concentration of 1741 ppmv (0.24 g/h), for 60 minutes, reduces the fungal presence of Alternaria sp., Fusarium sp., Aspergillus sp. and Penicillium sp. without affecting their physiological potential.

2020 ◽  
Vol 14 ◽  
Author(s):  
Alexsandra Cezimbra Quevedo ◽  
Marlove Fatima Brião Muniz ◽  
Clair Walker ◽  
Mateus Alves Saldanha

Research related to the pathology of seeds of native forest species, açoita-cavalo (Luehea divaricata), is scarce. This study aimed to evaluate the sanitary and physiological quality of whipshorse seeds and the possible transmission of fungi associated with the seeds to the seedlings, in lots from Santa Maria - RS. Were 100 seeds used, divided into four repetitions of 25 per lot (2016 and 2018) and test. The health test was carried out in which the seeds without asepsis were placed in “gerbox” boxes with filter paper substrate at a temperature of 25 °C, with a photoperiod of 12 h for seven days. The germination test and the fungi transmission test seeds were conducted in a germination room at a temperature of 25 ºC, and a photoperiod of 12 h. Only the seeds used in the germination test underwent asepsis. The evaluations of the two tests consisted of the First Germination Count (at 14 days) and the count in the number of normal, abnormal seedlings, and non-germinated seeds (at 28 days). In the health test, fungi were identified and quantified: Fusarium sp., Alternaria sp., Aspergillus sp., Penicillium sp., Rhizopus sp., Cladosporium sp., Pestalotia sp. and Epicoccum sp. The Fusarium genus was identified associated with the symptoms of browning and rotting in seedling roots in the transmission test. The sample from 2018 showed greater vigor, reaching 21% at 14 days, not different from the lot of 2016 that reached 10%. The lot of 2018 presented the best results concerning health, vigor, and germination.


2007 ◽  
pp. 261-265 ◽  
Author(s):  
Igor Stojanov ◽  
Sandra Jaksic ◽  
Jasna Prodanov

Dogs are animals that are most often kept as pets in the cities. Their health problem may be the cause of infections of humans and animals. Skin changes and etiology factors present important segment of the diseases that disturb health of the pets. The objective of this work was mycology examination of scarifications and skin swabs from dogs with clinical symptoms. The aim was to find out which fungi species can be isolated from the changed parts of the skin, and whether is possible that, besides dermatophyte, saprophyte fungi from the environment may also be the cause of the changes, and to reveal their effect on the host. During a one year period, 67 swabs and scarifications from dogs were examined to detect the presence of fungi. The samples were streaked on Sabourdaud's dextrose agar and incubated for 10-21 days at 25?C. In microscopis examination according to their shape, and color, the colonies were identified as conidia, macroconidia and conidiaophora. From 59, of total 67 samples, the following saprophyte fungi were isolated: Aspergillus sp., Penicillium sp., Alternaria sp., Mucor sp. and Fusarium sp. Occurrence of these fungi means that a considerable increase of this microbiological flora may be expected in homes of the owners. This may be the cause of systemic mycosis and allergies in animals and humans, as well as a possibility of contaminated food and incidence of mycotoxicosis.


2004 ◽  
Vol 26 (55) ◽  
Author(s):  
Rodrigo Makowiecky Stuart ◽  
Carolina Lamas ◽  
Ida Chapaval Pimentel

Este trabalho foi realizado para avaliar a ação de Trigona sp. como visitante floral e vetor de esporos fúngicos para goiabeira (Psidium guajava L.). As observações foram feitas entre dezembro de 2003 e fevereiro de 2004 em flores de goiabeiras do Setor de Ciências Biológicas, Universidade Federal do Paraná. Foi verificado que três gêneros de abelhas estavam visitando as flores de goiaba: Apis melifera, Bombus sp. e Trigona sp.. Entretanto, Trigona demonstrou ser mais freqüente que as outras durante as observações. A freqüência de visitas de Trigona foi maior durante o início da manhã, decrescendo ao longo do dia. A avaliação dos fungos associados a Trigona demonstrou a presença de 11 gêneros distintos: Acremonium sp., Altenaria sp., Aspergillus sp., Colletotrichum sp., Curvularia sp., Fonsecaea sp., Fusarium sp., Mycelia sterilia, Penicillium sp., Phoma sp. e Rhizopus sp.. 48 % destes representam fitopatógenos potenciais como Alternaria sp., Colletotrichum sp., Curvularia sp., Fusarium sp. e Phoma sp.. Estes dados demonstram que o gênero Trigona pode atuar na disseminação de doenças para diversas culturas, funcionando como vetor de esporos fúngicos para outras plantas.


2021 ◽  
Vol 12 (66) ◽  
Author(s):  
Merari Sujey Vazquez Lopez ◽  
Mario Ernesto Vazquez Badillo ◽  
Adriana Antonio Bautista ◽  
Arturo Mancera Rico

Las semillas de pinos durante su almacenamiento pueden presentar hongos que deterioran su calidad. El objetivo de la presente investigación consistió en identificar los géneros fúngicos que se asocian a las semillas de Pinus montezumae y P. greggii en dos humedades relativas (HR). Se les almacenó a 60 y 80 % de HR a 5 °C. Los muestreos se hicieron durante 180 días. Se evaluó el contenido de humedad de semilla (HS), las semillas libres de hongos (SLH); y se identificaron los géneros de los hongos (GH) por la morfología de sus colonias. En HS y SLH se aplicó un diseño experimental completamente al azar y se hizo una comparación de medias. Todas las fuentes de variación fueron significativas. La HS media por especie fue de 9.59 y de 12.37 %; la SLH de 64.52 y 69.28 % para P. greggii y P. montezumae, respectivamente. En la semilla de P. greggii almacenada a 60 HR, la HS fue de 7.97 %, con 66.19 % en SLH y a 80 HR, de 11.21 % y 62.85 % en SLH. Para P. montezumae a 60 HR, la HS fue de 10.21 % y de 71.42 % en SLH; a 80 HR, la HS fue de 14.53 % y 67.14 % en SLH. Los GH identificados que promueven el deterioro de las semillas fueron: Alternaria sp, que en P. greggii a 60 HR no se observó; Penicillium sp incrementó y predominó a partir de los 120 días; Fusarium sp. fue constante; de forma esporádica se presentaron Aspergillus sp y Rizhopus sp.


2021 ◽  
Author(s):  
Татьяна Стратулат ◽  
◽  
Татьяна Щербакова ◽  
Штефан Кручан ◽  
Андрей Лунгу ◽  
...  

To determine the complex of fungi that cause rot on the leaves and needles of tree species in the green spaces of Chisinau, foliage was collected in four sectors of the city. It was determined by microbio-logical methods that the complex of pathogens on the leaves differs little in different sectors. The main leaf rot fungi present on the affected leaves and needles are Alternaria sp., Aspergillus sp., Fusarium sp., Penicillium sp. For the treatment of the green spaces of Chisinau against diseases, it is advisable to carry out phytosanitary measures with biological products.


2006 ◽  
Vol 69 (7) ◽  
pp. 1724-1727 ◽  
Author(s):  
MANATCHAYA NGARMSAK ◽  
PASCAL DELAQUIS ◽  
PETER TOIVONEN ◽  
TIPVANNA NGARMSAK ◽  
BUNCHA OORAIKUL ◽  
...  

The antimicrobial activity of vanillin against four bacteria (Pantoea agglomerans, Aeromonas enteropelogenes, Micrococcus lylae, and Sphingobacterium spiritovorun), four fungi (Alternaria sp., Aspergillus sp., Penicillium sp., and Fusarium sp.), and three unidentified yeasts isolated from spoiling fresh-cut mango slices was verified in laboratory media adjusted to pH 5.0. MICs of vanillin against the fungi (12.5 to 13.3 mM), bacteria (10 to 13.3 mM), and yeasts (5.0 to 6.7 mM) indicated that all the test species were sensitive to the antimicrobial effects of vanillin. Fresh-cut mango slices were dipped for 1 min in solutions containing 40 and 80 mM vanillin before being packaged in rigid trays and stored at 5 and 10°C to verify the effects of vanillin on the development of the spoilage microflora. Microbiological analysis for up to 14 days of storage revealed that treatment with 80 mM vanillin significantly delayed (P < 0.05) the development of total aerobic bacteria and yeast and mold populations. Vanillin may be a practical preservative for processing fresh-cut mango.


2018 ◽  
Vol 13 (6) ◽  
pp. 191
Author(s):  
Raden Sujayadi ◽  
Supyani Supyani ◽  
Edi Purwanto

One of the obstacles in providing high quality soybean (Glycine max) seed is the infection of seed-borne fungal pathogens. Micro wave treatment is one of the alternative methods to control the seed-borne pathogens effectively but it needs to be further developed. This research consisted of two stages. The first stage was aimed to determine the best soybean seed moisture content in maintaining seed physiological quality after exposing to micro wave. The second stage was aimed to determine the most effective duration of micro wave exposure to reduce the rate of infection of seed-borne fungal pathogens using the best moisture content resulted from the first stage. The experiment  on seed moisture indicated that 9.20% was the best seed moisture content in maintaining the physiological quality when the seed was exposed to micro wave.  Furthermore, micro wave exposure of 60 seconds was able to control Aspergillus flavus, while the exposure of  80 seconds was able to control Fusarium sp., Curvularia sp., and A. niger.


2019 ◽  
Vol 3 (1) ◽  
pp. 1-14
Author(s):  
Erfan Dani Septia ◽  
Fitra Parlindo

Permintaan yang tinggi terhadap komoditas Kedelai seiring dengan peningkatan konsumsi masyarakat pada produk-produk olahannya. Kenyataan tersebut tidak diimbangi dengan peningkatan produktivitas kedelai dalam negeri. Riniarsi (2016) melaporkan bahwa produktivitas kedelai tahun 2016 bahkan mengalami penurunan sebesar 3.95% dibandingkan tahun sebelumnya.  Faktor yang menyebabkan penurunan produksi kedelai salah satunya karena penyakit yang disebabkan oleh virus tanaman. Seiring dengan tren pengurangan bahan kimia sintetis pada praktik budidaya tanaman, aplikasi mikroba endofit indigenous menjadi solusi alternatif dalam pengendalian penyakit yang disebabkan oleh virus. Penelitian ini bertujuan untuk mengetahui keanekaragaman mikroba endofit indigenous pada berbagai bagian jaringan tanaman kedelai dan menguji virulensinya secara in vitro.Penelitian ini menggunakan metode eksplorasi. Hasil eksplorasi cendawan endofit indigenous berjumlah 11 isolat dan bakteri berjumlah 3 isolat. Cendawan endofit indigenous berhasil diisolasi dari seluruh jaringan tanaman, kecuali polong. Keragaman cendawan endofit indigenous tertinggi terdapat pada jaringan akar dan batang, yaitu masing-masing berjumlah 4 isolat. Identitas cendawan endofit indigenous yang berhasil diidentifikasi antara lain adalah Fusarium sp., Verticilllum sp., Alternaria sp., Aspergillus sp., dan Penicillium sp. Adapun 6 isolat lainnya tidak dapat terindentifikasi. Bakteri endofit indigenous hanya terisolasi dari jaringan polong, akar, dan tanah. Seluruh bakteri merupakan golongan bakteri Gram negatif. Berdasarkan hasil Uji Hipovirulensi, terhadap 7 isolat cendawan endofit indigenous yang masuk dalam kategori hipovirulen dan 4 isolat lainnya bersifat virulen. Sedangkan semua isolat bakteri endofit indigenous yang diuji menunjukkan kategori virulen.


Author(s):  
Clair Walker ◽  
Ricardo Mezzomo ◽  
Caciara Gonzatto Maciel ◽  
Marlove Fátima Brião Muniz ◽  
Maristela Machado Araujo

A guajuvira é uma espécie florestal nativa amplamente distribuída no território brasileiro e possui demanda de pesquisas quanto à qualidade de suas sementes. Este trabalho teve como objetivos, avaliar a qualidade fisiológica e sanitária de sementes de guajuvira, coletadas na planta e após sua dispersão no solo em diferentes períodos. As variáveis analisadas foram: teor de água das sementes, matéria seca, germinação, vigor e sanidade de sementes. A qualidade sanitária foi determinada pela identificação dos microorganismos associados às sementes, dentre os quais foram identificados: Fusarium sp., Cladosporium sp., Alternaria sp., Colletotrichum sp., Aspergillus sp. e Penicillium sp. As sementes oriundas de frutos extraídos na planta atingiram valores superiores de germinação e vigor e a ocorrência de Fusarium sp. foi maior pois a associação do fungo pode ocorrer durante a maturação dos frutos.


Author(s):  
M. A. Nitu ◽  
M. Rahaman ◽  
F. M. Aminuzzaman ◽  
N. Sultana

Microflora from potato rhizosphere soil was isolated from different potato fields of Bangladesh. Seventeen soil samples were analyzed for the presence of microflora in selected potato field soils. Seven fungal species and one bacterium species were morphologically characterized using soil dilution and streak plate methods. The predominant fungi isolated including Alternaria sp., Aspergillus sp., Penicillium sp., Rhizopus sp., Bipolaris sp., Phytophthora sp., Fusarium sp. and one bacterium was identified as Ralstonia solanacearum. Individual colonies of fungi and bacteria were counted on Potato Dextrose Agar (PDA), V8 juice Agar and their presence in soil was compared in respect of different locations of potato fields. The occurrence of Phytophthora sp. was medium in Tongibari and lower in Singair Union, Sonargaon, Matlab Dakshin, Gobindaganj, Palashbari, Gopinathpur and Bagmara. The highest counts of R. solanacearum were found in Singair Union, Tongibari and Daudkandi and the lowest counts were made in Palashbari and Bagmara. This was the first reported examination of the microbial diversity of soil microflora in some selected potato fields of Bangladesh.


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