Controlled deterioration test and use of the Seed Vigor Imaging System (SVIS ® ) to evaluate the physiological potential of crambe seeds

Abstract: Vigor tests are important tools for verifying the physiological potential of seed lots; however, various aspects can interfere in the consistency of the test results. The search for less subjective procedures has drawn the interest of researchers. The aim of this study was to adapt the methodology of the controlled deterioration test and verify the efficiency of the Seed Vigor Imaging System (SVIS ® ) to evaluate the vigor of crambe seeds. Initially, seed moisture content was determined and germination and vigor were evaluated in five seed lots. For the controlled deterioration test, the seed moisture content was adjusted to 18%, 20%, and 22%, and the results were interpreted at four and five days after sowing. The controlled deterioration test should be performed with initial adjustment of seed moisture content to 18%, and the germination test should be evaluated on the fifth day after sowing. SVIS ® provided information on the vigor index and the length and uniformity of seedling development. Computerized image analysis is effective in evaluating the vigor of crambe seeds, and the uniformity of seedling development parameter offers the most consistent data.

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Accelerated aging and controlled deterioration for the determination of the physiological potential of onion seeds

Scientia Agricola ◽

10.1590/s0103-90162003000300008 ◽

2003 ◽

Vol 60 (3) ◽

pp. 465-469 ◽

Cited By ~ 12

Author(s):

Angelica Brod Rodo ◽

Julio Marcos Filho

Keyword(s):

Moisture Content ◽

Seedling Emergence ◽

Accelerated Aging ◽

Seed Vigor ◽

Seed Moisture Content ◽

Control Programs ◽

Traditional Procedure ◽

Accelerated Aging Test ◽

Seed Moisture ◽

Controlled Deterioration

International research on vegetable seed vigor is not at the same level attained for grain crops species. This study was conducted to identify reliable procedures for the accelerated aging and controlled deterioration tests to rank onion (Allium cepa L.) seed lots according to their physiological potential. Six seed lots of the cultivars Aurora and Petroline were evaluated in the laboratory for germination, first count, seedling vigor classification, traditional and saturated salt accelerated aging (41ºC / 48 and 72 h), controlled deterioration (24% of water / 45ºC / 24 h) and seedling emergence tests. Seed moisture content after the saturated salt accelerated aging test was lower and uniform, which is considered an important advantage in comparison to the traditional procedure. The saturated salt accelerated aging (41ºC / 48 and 72 h) and controlled deterioration (moisture content adjusted to 24% / 45ºC / 24 h) tests were the best procedures to assess the physiological potential of onion seeds, and are indicated for use in quality control programs.

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Controlled deterioration test for evaluation of sunn hemp seed vigor

Journal of Seed Science ◽

10.1590/2317-1545v37n4151816 ◽

2015 ◽

Vol 37 (4) ◽

pp. 249-253 ◽

Cited By ~ 1

Author(s):

Arthur Batoqui Costa e Silva ◽

Ícaro Monteiro Galvão ◽

Rafael Marani Barbosa ◽

Clíssia Barboza da Silva ◽

Roberval Daiton Vieira

Keyword(s):

Moisture Content ◽

Accelerated Aging ◽

Seed Vigor ◽

Germination Test ◽

Seed Moisture Content ◽

Hemp Seed ◽

Seed Moisture ◽

Sunn Hemp ◽

Controlled Deterioration ◽

Initial Seed

The controlled deterioration test is efficient for seed vigor evaluation of several species. However, the procedures conditions are incipient, especially in respect to tropical forages such as sunn hemp, which still does not have a standardized procedure. The objective of this research was to adjust the procedures for a controlled deterioration test in Crotalaria juncea L. seeds. For this, five commercial seed lots were evaluated for water content and initial physiological potential (germination test, first germination count, germination speed index, saturated salt accelerated aging with NaCl, electrical conductivity and seedling field emergence). For controlled deterioration test, the initial seed moisture content was adjusted to 18, 21 and 24% and, subsequently, the samples were exposed at 45 °C during 24 hours. Seed germination test was conducted with the use of sand as substrate. Evaluations were performed on the fourth, fifth and sixth day after sowing. The controlled deterioration test for sunn hemp seeds should be performed with 24% seed moisture content, at 45 °C during 24 hours, and the test evaluation on the fourth day after sowing.

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Uji Deteriorasi Terkontrol (UDT) ‎untuk Memperkirakan Masa Simpan Benih Kedelai (Glycine max (L.) Merr.)

Jurnal Agritech ◽

10.22146/agritech.34571 ◽

2019 ◽

Vol 39 (2) ◽

pp. 136

Author(s):

Pepi Nur Susilawati ◽

Anis Andrini ◽

Evi Dwi Sulistya Nugroho ◽

Ulima ‎ Darmania Amanda

Keyword(s):

Moisture Content ◽

Heat Exposure ◽

Soybean Seed ◽

Storage Period ◽

Seed Vigor ◽

Limiting Factor ◽

Seed Moisture Content ◽

Seed Moisture ◽

Vigor Index ◽

Split Plot

Deterioration of seeds during storage may reduce the supply of high-quality seeds which become the limiting ‎factor of soybean production in tropical countries. Controlled Deterioration Test (CDT) is one of the ‎fastest vigor testing methods. The objective of this study was to achieve the suitable moisture content and ‎duration of CDT treatment in soybean seed, then establish a model that associated with the seed vigor in ‎the field after a certain time of storage. Split-plot with 2 factors, namely seed varieties and the combination of ‎moisture content and heat exposure duration were used to determine the influence of CDT treatment. ‎Another split-plot for 2 factors, namely storage period and seed varieties were used to determine the effect ‎of shelf life. Viability parameters were observed by germination strength (GS) and seedling vigor index (VI), ‎using a rolled paper towel method. The results showed that there was an interaction between soybean seed ‎varieties and the combination of seed moisture content treatment with duration of CDT exposure. Sindoro ‎and Tanggamus varieties had higher viability and storability than other varieties. Combinations of 28% seed ‎moisture content for 24 hours CDT duration were sensitive enough to evaluate the physiological potential of ‎soybean seeds, providing information that was closely related to seed germination after stored for 1 month. ‎

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Composition and Characteristics of Blended Kentucky Bluegrass Stands

HortScience ◽

10.21273/hortsci.37.7.1124 ◽

2002 ◽

Vol 37 (7) ◽

pp. 1124-1126 ◽

Cited By ~ 3

Author(s):

Darin W. Lickfeldt ◽

Thomas B. Voigt ◽

Andrew M. Hamblin

Keyword(s):

Moisture Content ◽

Dna Markers ◽

Poa Pratensis ◽

Growing Season ◽

Kentucky Bluegrass ◽

Seedling Development ◽

Initial Composition ◽

Seed Moisture Content ◽

Seed Moisture ◽

Percent Germination

Kentucky bluegrass (Poa pratensis L.) cultivars are often blended to incorporate diverse characteristics. Factors that may contribute to the actual cultivar composition have not been evaluated. Through the use of DNA markers, individual plants in blended stands can be identified. This study evaluated changes in cultivar composition of `Blacksburg', `Midnight', and `Unique' Kentucky bluegrass blends. Characteristics such as seed size, seed moisture content, percent germination, and seedling development did not affect the initial composition of blends at time of seeding. DNA markers were used to demonstrate how the composition of a blended `Blacksburg', `Midnight', and `Unique' turf changed during the first growing season following establishment. The composition of blends did not significantly change from time of seeding in Sept. 1999 to Apr. 2000 or from Apr. 2000 to Oct. 2000. Two of the three blends were significantly different by Oct. 2000 relative to the percentages seeded in Sept. 1999.

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CONTROLLED DETERIORATION TEST AS VIGOR ASSESSMENTS IN PEPPER SEED LOTS: 1. DETERMINATION OF APPROPRIATE SEED MOISTURE CONTENT

Acta Horticulturae ◽

10.17660/actahortic.2007.729.22 ◽

2007 ◽

pp. 145-149

Author(s):

S. Kavak ◽

H. Ilbi ◽

B. Eser ◽

I. Duman

Keyword(s):

Moisture Content ◽

Seed Moisture Content ◽

Seed Moisture ◽

Controlled Deterioration ◽

Pepper Seed

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RESPONSE OF DIFFERENT STORAGE CONTAINERS IN RICE GENOTYPES DURING STORAGE

American International Journal of Agricultural Studies ◽

10.46545/aijas.v4i1.277 ◽

2021 ◽

pp. 21-35

Keyword(s):

Moisture Content ◽

Seed Quality ◽

Aluminum Foil ◽

Germination Percentage ◽

Seed Moisture Content ◽

Storage Container ◽

Seed Moisture ◽

Vigor Index ◽

Rice Genotypes ◽

Good Quality Seed

Rice is the staple food of more than half the world’s population and is being cultivated in Asia since ancient time’s generation after generation. Good quality seeds are the seeds of the green revolution, it has been assertively and emphatically shown that 15-20% increased yield could be achieved by the use of good quality seeds alone. Good quality seed is one of the most important requirements for production and supply to achieve higher production and productivity in any crop. The seed quality is depended on various factors Viz. genetic, edaphic, environmental, biotic, etc. Good quality seed production depends on complex conditions evoking the most favorable interactions between the genetic makeup of the seed and the environment, under which it is produced, harvested, processed, and stored. Seeds undergo deterioration at various levels during storage resulting in a decline in vigor and viability. With the above considerations, the present investigation has been undertaken to observe the potentials of ten rice genotypes and observed its seed quality after harvesting in 2016-2017 at Seed Testing Laboratory, Department of Seed Science and Technology, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal, India. Just after harvesting G2 (Gobindabhog) and G9 (IR-36) showed highest germination percentage (97.66) and G9 (IR-36) showed highest Vigor index (3204.13). But most of the varieties germination and vigor were nearer to G2 & G9. Among the containers with seed moisture content aluminum foil packet with seed moisture content, 8% is the best storage container for rice among the other containers as up to one-year storage vigor index was observed highest in that conditions.

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Seed germination and seedling development in Taro (Colocasia esculenta)

The South Pacific Journal of Natural and Applied Sciences ◽

10.1071/sp04007 ◽

2004 ◽

Vol 22 (1) ◽

pp. 62

Author(s):

A P Tyagi ◽

M Taylor ◽

P C Deo

Keyword(s):

Seed Germination ◽

Moisture Content ◽

Seed Storage ◽

Seedling Development ◽

Colocasia Esculenta ◽

Day Length ◽

Seed Moisture Content ◽

Air Drying ◽

Seed Moisture ◽

Hand Pollination

Two taro (Colocasia esculenta (L.) Schott var. esculenta) cultivars from Fiji and Papua New Guinea were grown at the University of the South Pacific, Laucala Campus, Fiji to produce seeds for seed storage experiments. Gibberellic acid at a 500ppm concentration was used to induce flowering. Very few flowering shoots (inflorescence) were observed in the Fiji cultivar and all pollinations were unsuccessful. However the PNG cultivar flowered well and was used to obtain seed after hand pollination. Hand pollination was carried out to ensure seed setting in developing fruits in the inflorescence. Seeds were extracted in the laboratory after harvesting mature inflorescences. Experiments were conducted on seed moisture content, desiccation, germination, seedling development and seed storage behaviour of taro (Colocasia esculenta) seeds. Seed moisture content was determined using oven methods and air-drying. Results demonstrated that taro seeds have a moisture content of 12-13% after air-drying for three to four weeks. Seeds were dried to desired moisture contents in a desiccator over silica gel. After drying to 5% moisture content seed viability was tested by germinating seeds on moist filter paper at room temperature with 65% relative humidity and seven to eight hours day length. Preliminary seed germination tests demonstrated up to 83% germination for seeds with 13% moisture content. Germination occurred within five to seven days. Maximum germination was achieved within 21 days. The highest germination (80%) was achieved with seeds with 12% moisture content. Results indicated there was no relationship between moisture content and seed germination. Normal seedling development and growth was recorded after germination.

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Use of Digital Analysis of Radicle Extension of Marigold Seedlings as an Early Indicator of Seed Vigor

HortScience ◽

10.21273/hortsci.33.3.467b ◽

1998 ◽

Vol 33 (3) ◽

pp. 467b-467

Author(s):

Kay Oakley ◽

Robert Geneve ◽

Gunching Siriwitayawan ◽

Sharon Kester

Keyword(s):

Moisture Content ◽

Seedling Emergence ◽

Accelerated Aging ◽

Seed Vigor ◽

Digital Analysis ◽

Germination Test ◽

Seed Moisture Content ◽

Seed Moisture ◽

Aged Seed ◽

Radicle Length

Several seed vigor tests were conducted on Marigold `Little Devil Flame' and correlated with digital analysis of radicle extension. Seed from a single lot was deteriorated by the accelerated aging (AA) method at 41 °C for 24 and 72 h. Seed moisture content of unaged seed was 15.23%, mildly aged seed (24-h AA) was 40.77%, and moderately aged seed (72-h AA) was 52.85%. After AA treatment, all seeds were dried to near 15% moisture. Vigor level was assessed 7 days after AA treatment by standard germination and soilless-mix plug emergence tests according to the Association of Official Seed Analysts Rules for Testing Seed. The standard germination test revealed an 84.5% germination for unaged seed, 49.5% germination for mildly aged seed, and 28.5% germination for moderately aged seed. The plug emergence test showed an 88.9% normal emergence for unaged seed, 65.28% germination for mildly aged seed, and 9.72% germination for moderately aged seed. Additionally, the radicle of seedlings established under standard germination conditions was digitally analyzed after 4 days. According to digital analysis, average radicle length for unaged seed was 2.10 cm, 1.91 cm, for mildly aged seed, and 1.27 cm for moderately aged seed. Radicle length after 4 days showed an R2 of 0.81 when regressed against standard germination and an R2 of 0.99 with seedling emergence tests. These results suggest that digital analysis of radicle extension could provide a sensitive, reproducible test for seed vigor.

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