scholarly journals Seed germination and seedling development in Taro (Colocasia esculenta)

2004 ◽  
Vol 22 (1) ◽  
pp. 62
Author(s):  
A P Tyagi ◽  
M Taylor ◽  
P C Deo

Two taro (Colocasia esculenta (L.) Schott var. esculenta) cultivars from Fiji and Papua New Guinea were grown at the University of the South Pacific, Laucala Campus, Fiji to produce seeds for seed storage experiments. Gibberellic acid at a 500ppm concentration was used to induce flowering. Very few flowering shoots (inflorescence) were observed in the Fiji cultivar and all pollinations were unsuccessful. However the PNG cultivar flowered well and was used to obtain seed after hand pollination. Hand pollination was carried out to ensure seed setting in developing fruits in the inflorescence. Seeds were extracted in the laboratory after harvesting mature inflorescences. Experiments were conducted on seed moisture content, desiccation, germination, seedling development and seed storage behaviour of taro (Colocasia esculenta) seeds. Seed moisture content was determined using oven methods and air-drying. Results demonstrated that taro seeds have a moisture content of 12-13% after air-drying for three to four weeks. Seeds were dried to desired moisture contents in a desiccator over silica gel. After drying to 5% moisture content seed viability was tested by germinating seeds on moist filter paper at room temperature with 65% relative humidity and seven to eight hours day length. Preliminary seed germination tests demonstrated up to 83% germination for seeds with 13% moisture content. Germination occurred within five to seven days. Maximum germination was achieved within 21 days. The highest germination (80%) was achieved with seeds with 12% moisture content. Results indicated there was no relationship between moisture content and seed germination. Normal seedling development and growth was recorded after germination.

2019 ◽  
Vol 6 (2) ◽  
pp. 243-251
Author(s):  
M Anto ◽  
M Angala ◽  
P S Jothish ◽  
C Anilkumar

Garcinia imberti seeds were collected during 2015-2017 from Shangili, Cheenikkala and Bonaccord evergreen forests of Agasthyamala Biosphere Reserve, the only abode of this endangered endemic species. Germinability of seeds were analysed through decoating, Gibberellic acid (GA3) and light inductive pre-treatments on fresh (62.8 % moisture content; MC) and desiccated (fast; 23.3% MC and slow; 30.5 % MC) seeds. The seed germination with impermeable coat (0.7-1.2 mm) was restricted which on decoating got enhanced. Application of GA3 along with exposure to light breaked dormancy within 4-6 days compared to non-treated seeds that took 238-254 days to germinate. Stored seeds behaviour revealed that seed moisture content and rate of germination were negatively correlated. Seed storage was found to be more efficient only up to 80 days at controlled seed banking conditions (20 ± 20C, 20 % relative humidity; RH). Both fast and slow desiccated seeds stored for 60 days in seed bank conditions exhibited 50.4 and 43.4 % of germination compared 39.4% germination of non-desiccated seeds. Hence fast desiccated and decoated G. imberti seeds pre-treated with GA3 on subsequent exposure to light alleviated dormancy. For seed banking, fast desiccated seeds with MC in between 40-20% are found to be promising.


2016 ◽  
Vol 34 (3) ◽  
pp. 219
Author(s):  
Ari Wahyuni ◽  
M. R. Suhartanto ◽  
Abdul Qadir

<p>Soybean seed viability declines during seed storage. Soybean seed deteriorates rapidly, affected by its high protein content and often high humidity in the tropical environment. This research was aimed to develop dynamic model of soybean seed viability in an open storage. The study was conducted in three stages, namely: 1) desk study, 2) seed storage experiment, 3) development of seed storage model, simulation and verification of the model. The second stage of the experiment consisted of soybean seed storing and germination testing using completely randomized design. Treatments were three initial moisture content (7-8%, 9-10% and 11-12%) and four varieties of soybean (Anjasmoro, Wilis, Detam-1 and Detam-2). The results showed that the seed behaviour during storage period were affected by initial seed moisture content, initial viability, varieties and environmental condition. Therefore, seed moisture content, initial viability and varieties may be used as input model. Moisture content, integreting seed respiration, electric conductivity and seed storability vigor (VDSDB) were as model output. Simulation of Seed Storability Vigor Prediction Model with Model Construction Layer-Stella (MCLS) using relative humidity (RH), temperature, seed permeability, initial moisture content and initial viability as input model could logically predict the seed moisture content and seed storability vigor (VDSDB).</p>


HortScience ◽  
1995 ◽  
Vol 30 (5) ◽  
pp. 1003-1006 ◽  
Author(s):  
William J. Carpenter ◽  
Eric R. Ostmark ◽  
John A. Cornell

Various combinations of temperature and moisture contents were used in evaluating the seed storage of nine genera of annual flowers. Relative humidity (RH) levels of 11%, 32%, 52%, and 75% provided wide ranges in seed moisture during storage at 5, 15, and 25C. At each temperature, total germination percentages (G) generally declined as seed moisture content increased during storage. The seed moisture range giving the highest G after 12 months of storage was determined for each temperature and plant genus. For all genera, seed moisture contents during storage increased as storage temperatures increased at constant RH levels. Moisture contents at 25C storage were 37%, 34%, 29%, and 20% higher than at 5C when RH levels were at 11%, 32%, 52%, and 75%, respectively.


HortScience ◽  
2002 ◽  
Vol 37 (7) ◽  
pp. 1124-1126 ◽  
Author(s):  
Darin W. Lickfeldt ◽  
Thomas B. Voigt ◽  
Andrew M. Hamblin

Kentucky bluegrass (Poa pratensis L.) cultivars are often blended to incorporate diverse characteristics. Factors that may contribute to the actual cultivar composition have not been evaluated. Through the use of DNA markers, individual plants in blended stands can be identified. This study evaluated changes in cultivar composition of `Blacksburg', `Midnight', and `Unique' Kentucky bluegrass blends. Characteristics such as seed size, seed moisture content, percent germination, and seedling development did not affect the initial composition of blends at time of seeding. DNA markers were used to demonstrate how the composition of a blended `Blacksburg', `Midnight', and `Unique' turf changed during the first growing season following establishment. The composition of blends did not significantly change from time of seeding in Sept. 1999 to Apr. 2000 or from Apr. 2000 to Oct. 2000. Two of the three blends were significantly different by Oct. 2000 relative to the percentages seeded in Sept. 1999.


Author(s):  
M.Yu. Skorokhodov ◽  
R.L. Boguslavskyi ◽  
V.S. Lyutenko

Аim. To determine the optimum moisture content and comparative longevity of seeds of wheat species accessions for long-term storage according to the results of the “accelerated seed aging” experiment. Results and Discussion. There was estimated seed longevity of the representatives of wheat species Triticum aestivum L., T. spelta L., T. durum Desf., T. dicoccum (Schrank) Schuebl., T. monococcum L., T. sinskajae A. Filat. et Kurk. of three reproduction years – 2014, 2016 and 2017, at three moisture levels – 5 %, 6 % and 7 % in the experiment with accelerated aging. Germination energy and germination rate depended on the year conditions, seed moisture, the interactions year – genotype, year – seed moisture and all three factors: year conditions, seed moisture, genotype (influence power from 16.1 to 20.1). Accelerated aging caused decrease in seed germination energy and germination rate in almost all samples at all three moisture levels. Under the accelerated aging, with an increase in seed moisture content from 5 % to 7 %, on average by the accessions, the seed germination energy (from 83 % to 63 %) and germination rate (from 90 % to 68 %) decreased on samples. At all three moisture levels, relatively high germination energy and germination rate after accelerated aging had T.monococcum (86-95 % and 88-97 %) and Polba 3 (74-96 % and 85-98 %). A low germination energy (66-69 % и 68-77 %) and germination rate showed T.sinskajae UA0300224 (66-69 %) and T.durum Spadschyna (56-75 % и 61-92 %). At all three seed moisture levels, the high plasticity of germination energy had T.sinskajae UA0300224 and T.durum Spadschina, germination rate – T.sinskajae. With all three seed moisture levels, T.sinskajae UA0300224 and T.aestivum Harkivska 26 were characterized by stable germination rate. Conclusions. The highest seed longevity of the studied accessions is provided by moisture of 5%, less but close longevity – the moisture of 6%. The least longevity in three test options had seeds with 7 % moisture content. Among studied accessions of wheat species most longevous seeds had T.monococcum UA0300439 and Polba 3. The seeds of T.sinskajae UA0300224 and T.astivum PI619379 were less longevous.


1981 ◽  
Vol 23 (2) ◽  
pp. 267-280 ◽  
Author(s):  
Minoru Murata ◽  
Eric E. Roos ◽  
Takumi Tsuchiya

In order to study the genetic changes which occur during seed storage, barley (Hordeum vulgare L. 'Himalaya') seeds were subjected to artificial aging using six combinations of temperature (21 °C, 32 °C, and 38 °C) and seed moisture content (12% and 18%). With increasing time in storage, germination of the seeds was delayed and reduced. Abnormal seedlings without roots also occurred with increased storage. Higher temperature and seed moisture content induced rapid loss of germinability. At the first mitotic division in the root tips, the frequency of aberrant anaphases and of roots with aberrations increased with increased storage time. The frequencies of aberrant anaphases and of roots with aberrations were also increased by higher temperature and seed moisture content. Frequencies of aberrant anaphases and of roots with aberrations were negatively correlated with germination percentage. This indicated that the frequency of chromosomal aberrations induced by seed aging might be estimated from the germination percentages.


2020 ◽  
Vol 9 (9) ◽  
pp. e858998157
Author(s):  
Érica Coutinho David ◽  
Bressa Karolina Dias Cardoso ◽  
Josiene Amanda dos Santos Viana ◽  
Eniel David Cruz

Knowledge about seed storage behavior is important to preserve plant species. Clitoria fairchildiana R.A.Howard is an endemic species with medicinal properties, it is used in the recovery of degraded land. The objective of this study is to evaluate the effect of drying on the physiological quality of C. fairchildiana seeds. Seeds were oven dried along with silica gel for 18 days and in intervals of 2 or 3 days, samples were removed to assess the seed moisture content. This was done in an oven at 105±3 oC for 24h, using four replicates with 10 seeds each. After undergoing the above-mentioned treatment, a sample of seeds was stored in the freezer (-18 ºC) for 3 months. Sowing was carried out in plastic trays in a sand and sawdust mixture (1:1), previously sterilized in hot water (100 oC) for two hours. Seeds were left to germinate in a laboratory with no temperature and relative humidity control. Germination tests were performed over 14 days with daily counts of the number of emerged seedlings. The percentage of seed germination, abnormal seedlings and dead seeds were obtained 14 days after sowing. The experimental design was completely randomized with four replications of 25 seeds. The reduction of seed moisture content from 18.5% to 5.6% affected physiological seed quality causing a reduction in the germination percentage and an increase in dead seeds and abnormal seedling percentage. C. fairchildiana seeds are classified as intermediate and they can be desiccated up to 8.6% with no reduction in physiology quality.


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