Use of Digital Analysis of Radicle Extension of Marigold Seedlings as an Early Indicator of Seed Vigor

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 467b-467
Author(s):  
Kay Oakley ◽  
Robert Geneve ◽  
Gunching Siriwitayawan ◽  
Sharon Kester
Keyword(s):  
Moisture Content ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Digital Analysis ◽  
Germination Test ◽  
Seed Moisture Content ◽  
Seed Moisture ◽  
Aged Seed ◽  
Radicle Length

Several seed vigor tests were conducted on Marigold `Little Devil Flame' and correlated with digital analysis of radicle extension. Seed from a single lot was deteriorated by the accelerated aging (AA) method at 41 °C for 24 and 72 h. Seed moisture content of unaged seed was 15.23%, mildly aged seed (24-h AA) was 40.77%, and moderately aged seed (72-h AA) was 52.85%. After AA treatment, all seeds were dried to near 15% moisture. Vigor level was assessed 7 days after AA treatment by standard germination and soilless-mix plug emergence tests according to the Association of Official Seed Analysts Rules for Testing Seed. The standard germination test revealed an 84.5% germination for unaged seed, 49.5% germination for mildly aged seed, and 28.5% germination for moderately aged seed. The plug emergence test showed an 88.9% normal emergence for unaged seed, 65.28% germination for mildly aged seed, and 9.72% germination for moderately aged seed. Additionally, the radicle of seedlings established under standard germination conditions was digitally analyzed after 4 days. According to digital analysis, average radicle length for unaged seed was 2.10 cm, 1.91 cm, for mildly aged seed, and 1.27 cm for moderately aged seed. Radicle length after 4 days showed an R2 of 0.81 when regressed against standard germination and an R2 of 0.99 with seedling emergence tests. These results suggest that digital analysis of radicle extension could provide a sensitive, reproducible test for seed vigor.

scholarly journals Accelerated aging and controlled deterioration for the determination of the physiological potential of onion seeds

2003 ◽  
Vol 60 (3) ◽  
pp. 465-469 ◽  
Author(s):  
Angelica Brod Rodo ◽  
Julio Marcos Filho
Keyword(s):  
Moisture Content ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Seed Moisture Content ◽  
Control Programs ◽  
Traditional Procedure ◽  
Accelerated Aging Test ◽  
Seed Moisture ◽  
Controlled Deterioration

International research on vegetable seed vigor is not at the same level attained for grain crops species. This study was conducted to identify reliable procedures for the accelerated aging and controlled deterioration tests to rank onion (Allium cepa L.) seed lots according to their physiological potential. Six seed lots of the cultivars Aurora and Petroline were evaluated in the laboratory for germination, first count, seedling vigor classification, traditional and saturated salt accelerated aging (41ºC / 48 and 72 h), controlled deterioration (24% of water / 45ºC / 24 h) and seedling emergence tests. Seed moisture content after the saturated salt accelerated aging test was lower and uniform, which is considered an important advantage in comparison to the traditional procedure. The saturated salt accelerated aging (41ºC / 48 and 72 h) and controlled deterioration (moisture content adjusted to 24% / 45ºC / 24 h) tests were the best procedures to assess the physiological potential of onion seeds, and are indicated for use in quality control programs.

scholarly journals Controlled deterioration test for evaluation of sunn hemp seed vigor

2015 ◽  
Vol 37 (4) ◽  
pp. 249-253 ◽  
Author(s):  
Arthur Batoqui Costa e Silva ◽  
Ícaro Monteiro Galvão ◽  
Rafael Marani Barbosa ◽  
Clíssia Barboza da Silva ◽  
Roberval Daiton Vieira
Keyword(s):  
Moisture Content ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Germination Test ◽  
Seed Moisture Content ◽  
Hemp Seed ◽  
Seed Moisture ◽  
Sunn Hemp ◽  
Controlled Deterioration ◽  
Initial Seed

The controlled deterioration test is efficient for seed vigor evaluation of several species. However, the procedures conditions are incipient, especially in respect to tropical forages such as sunn hemp, which still does not have a standardized procedure. The objective of this research was to adjust the procedures for a controlled deterioration test in Crotalaria juncea L. seeds. For this, five commercial seed lots were evaluated for water content and initial physiological potential (germination test, first germination count, germination speed index, saturated salt accelerated aging with NaCl, electrical conductivity and seedling field emergence). For controlled deterioration test, the initial seed moisture content was adjusted to 18, 21 and 24% and, subsequently, the samples were exposed at 45 °C during 24 hours. Seed germination test was conducted with the use of sand as substrate. Evaluations were performed on the fourth, fifth and sixth day after sowing. The controlled deterioration test for sunn hemp seeds should be performed with 24% seed moisture content, at 45 °C during 24 hours, and the test evaluation on the fourth day after sowing.

scholarly journals Physiological potential of soybean seeds after maturation and submitted to artificial drying

2017 ◽  
Vol 39 (4) ◽  
pp. 374-384
Author(s):  
Cesar Pedro Hartmann Filho ◽  
André Luís Duarte Goneli ◽  
Tathiana Elisa Masetto ◽  
Elton Aparecido Siqueira Martins ◽  
Guilherme Cardoso Oba
Keyword(s):  
Moisture Content ◽  
Dry Matter ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Maturation Stage ◽  
Soybean Seeds ◽  
Germination Test ◽  
Moisture Contents ◽  
Three Samples ◽  
Maturation Stages

Abstract: This study evaluated the physiological potential of soybean seeds harvested during two seasons, on different maturation stages and subjected to different drying temperatures. The seeds were harvested at the maturations stages R7, R7 + 2, R7 + 3, R7 + 5, R7 + 6, R7 + 7, R7 + 10 and R7 + 12 days (55, 50, 45, 40, 35, 30, 25, and 20% of moisture content). For each maturation stage, seeds were divided into three samples: one sample was used to directly evaluate the physiological potential, and the others were dried at 40 °C and 50 °C, until reaching the moisture content of 11.5%. The physiological potential was evaluated through germination test, first germination count of germination, accelerated aging, modified cold, electrical conductivity and seedling emergence. The maximum physiological potential of seeds is achieved at the moisture content of 55%, the point that the dry matter is maximum. The seeds became tolerant to artificial drying approximately at the stage R7 + 7 days (30% of moisture content). Germination and vigor of the soybean seeds reduce as the drying temperature is increased from 40 °C to 50 °C, and this effect is enhanced when the seeds show moisture contents above 30%.

scholarly journals ELECTRICAL CONDUCTIVITY AND ETHANOL RELEASE TO ASSESS RED RICE SEED VIGOR1

2021 ◽  
Vol 34 (4) ◽  
pp. 791-798
Author(s):  
RAFAEL MARANI BARBOSA ◽  
MATHEUS ANDRÉ DE JESUS ◽  
RAFAELA ALVES PEREIRA ◽  
GEDEON ALMEIDA GOMES JUNIOR
Keyword(s):  
Electrical Conductivity ◽  
Efficient Method ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Rice Seed ◽  
Seed Sample ◽  
Red Rice ◽  
Germination Test ◽  
Rice Seeds

ABSTRACT To evaluate seed vigor, electrical conductivity and ethanol tests are fast and efficient methodologies. They have the potential to be used in several species, such as red rice. However, there are no protocols or information about their efficiency. Thus, the objective was to evaluate the efficiency, and define parameters of execution for electrical conductivity and ethanol tests, to evaluate the vigor of red rice seeds. The study was conducted using four lots of ‘BRS 901’ red rice, which was subjected to a germination test, as well as first count, accelerated aging, and field seedling emergence tests. The electrical conductivity test was performed with 25, 50, and 100 seeds soaked in 50 mL and 75 mL of water, at 25 °C and 30 °C, for 3, 6, 20, and 24 hours, respectively. The ethanol test was performed with 50 and 100 seeds soaked in a volume of water equivalent to 1.0, 1.5, 2.0, 2.5, and 3.0× the mass of the seed sample. To assess the vigor of red rice seeds, the electrical conductivity test is an efficient method when conducted with 50 seeds soaked in 50 mL of water at 25 °C for 20 hours. Meanwhile, the ethanol test is most effective when performed with 50 seeds, in a volume of water that is 2.5× the mass of the sample, at 40 °C for 24 hours.

scholarly journals Improved assessment of wheat seeds vigor

2012 ◽  
Vol 36 (6) ◽  
pp. 608-614 ◽  
Author(s):  
Tereza Cristina de Carvalho ◽  
Francisco Carlos Krzyzanowski ◽  
Osvaldo de Castro Ohlson ◽  
Maristela Panobianco
Keyword(s):  
Moisture Content ◽  
Filter Paper ◽  
Seed Quality ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Wheat Seed ◽  
Efficient Procedure ◽  
Tetrazolium Test ◽  
Wheat Seeds

The tetrazolium test is widely used for seed vigor assessment of various plant species. For wheat, however, there is no methodology specifically recommended. This study aimed at determining an efficient procedure to evaluate vigor of wheat seeds by this test; besides allowing the establishment of seed quality classes. Thus, four different wheat seed lots were assessed by determining the moisture content and through tests of germination, accelerated aging, and field seedling emergence. The following methodologies were assessed: 1) longitudinal bisection of seed, with subsequent placement of the two halves on filter paper moistened with a 1.0% tetrazolium solution, at 30º C, for 2 h; 2) longitudinal bisection, with subsequent immersion of one seed half into a 1.0% tetrazolium solution, at 30º C, for 3 h; and 3) longitudinal bisection, with subsequent immersion of one seed half into a 0.075% tetrazolium solution, at 40º C, for 2 h. It was concluded that the tetrazolium test is more efficient in evaluating wheat seed vigor when performed with immersion of one half of the seed into a 0.075% tetrazolium solution (40º C, for 2 h) or a 0.1% tetrazolium solution (30º C, during 3 h); allowing to sort seeds into four quality classes.

EFFECT OF SEED MOISTURE CONTENT AT SWATHING ON EMERGENCE OF FALL RYE

1978 ◽  
Vol 58 (2) ◽  
pp. 557-560
Author(s):  
R. M. DE PAUW
Keyword(s):  
Moisture Content ◽  
Secale Cereale ◽  
Seed Treatment ◽  
Seedling Emergence ◽  
Seed Moisture Content ◽  
Moisture Contents ◽  
Seed Moisture ◽  
Secale Cereale L ◽  
The Absolute ◽  
Absolute Duration

The after-ripening requirement of three fall rye (Secale cereale L.) cultivars, harvested at three seed moisture contents (50, 42, and 32%), was assessed by monitoring the changes in seedling emergence from sowings made over a period of 60 days. All cultivars exhibited a short after-ripening requirement of 1–7 days. The absolute duration of the after-ripening requirement was not affected by the range of seed moisture contents studied. Seed treatment with captan [N[(trichloromethyl) thiol]-4-cyclohexene-1,2-dicarboximide] significantly increased percent seedling emergence. The captan treatment had the greatest effect on seeds harvested at the lowest moisture content.

scholarly journals Uji Deteriorasi Terkontrol (UDT) ‎untuk Memperkirakan Masa Simpan Benih Kedelai (Glycine max (L.) Merr.)

2019 ◽  
Vol 39 (2) ◽  
pp. 136
Author(s):  
Pepi Nur Susilawati ◽  
Anis Andrini ◽  
Evi Dwi Sulistya Nugroho ◽  
Ulima ‎ Darmania Amanda
Keyword(s):  
Moisture Content ◽  
Heat Exposure ◽  
Soybean Seed ◽  
Storage Period ◽  
Seed Vigor ◽  
Limiting Factor ◽  
Seed Moisture Content ◽  
Seed Moisture ◽  
Vigor Index ◽  
Split Plot

Deterioration of seeds during storage may reduce the supply of high-quality seeds which become the limiting ‎factor of soybean production in tropical countries. Controlled Deterioration Test (CDT) is one of the ‎fastest vigor testing methods. The objective of this study was to achieve the suitable moisture content and ‎duration of CDT treatment in soybean seed, then establish a model that associated with the seed vigor in ‎the field after a certain time of storage. Split-plot with 2 factors, namely seed varieties and the combination of ‎moisture content and heat exposure duration were used to determine the influence of CDT treatment. ‎Another split-plot for 2 factors, namely storage period and seed varieties were used to determine the effect ‎of shelf life. Viability parameters were observed by germination strength (GS) and seedling vigor index (VI), ‎using a rolled paper towel method. The results showed that there was an interaction between soybean seed ‎varieties and the combination of seed moisture content treatment with duration of CDT exposure. Sindoro ‎and Tanggamus varieties had higher viability and storability than other varieties. Combinations of 28% seed ‎moisture content for 24 hours CDT duration were sensitive enough to evaluate the physiological potential of ‎soybean seeds, providing information that was closely related to seed germination after stored for 1 month. ‎

Moisture level effect on seed longevity of wheat species representatives

2019 ◽  
pp. 121-128
Author(s):  
M.Yu. Skorokhodov ◽  
R.L. Boguslavskyi ◽  
V.S. Lyutenko
Keyword(s):  
Seed Germination ◽  
Moisture Content ◽  
Germination Rate ◽  
Accelerated Aging ◽  
Seed Longevity ◽  
High Plasticity ◽  
Wheat Species ◽  
Seed Moisture Content ◽  
Germination Energy ◽  
Seed Moisture

Аim. To determine the optimum moisture content and comparative longevity of seeds of wheat species accessions for long-term storage according to the results of the “accelerated seed aging” experiment. Results and Discussion. There was estimated seed longevity of the representatives of wheat species Triticum aestivum L., T. spelta L., T. durum Desf., T. dicoccum (Schrank) Schuebl., T. monococcum L., T. sinskajae A. Filat. et Kurk. of three reproduction years – 2014, 2016 and 2017, at three moisture levels – 5 %, 6 % and 7 % in the experiment with accelerated aging. Germination energy and germination rate depended on the year conditions, seed moisture, the interactions year – genotype, year – seed moisture and all three factors: year conditions, seed moisture, genotype (influence power from 16.1 to 20.1). Accelerated aging caused decrease in seed germination energy and germination rate in almost all samples at all three moisture levels. Under the accelerated aging, with an increase in seed moisture content from 5 % to 7 %, on average by the accessions, the seed germination energy (from 83 % to 63 %) and germination rate (from 90 % to 68 %) decreased on samples. At all three moisture levels, relatively high germination energy and germination rate after accelerated aging had T.monococcum (86-95 % and 88-97 %) and Polba 3 (74-96 % and 85-98 %). A low germination energy (66-69 % и 68-77 %) and germination rate showed T.sinskajae UA0300224 (66-69 %) and T.durum Spadschyna (56-75 % и 61-92 %). At all three seed moisture levels, the high plasticity of germination energy had T.sinskajae UA0300224 and T.durum Spadschina, germination rate – T.sinskajae. With all three seed moisture levels, T.sinskajae UA0300224 and T.aestivum Harkivska 26 were characterized by stable germination rate. Conclusions. The highest seed longevity of the studied accessions is provided by moisture of 5%, less but close longevity – the moisture of 6%. The least longevity in three test options had seeds with 7 % moisture content. Among studied accessions of wheat species most longevous seeds had T.monococcum UA0300439 and Polba 3. The seeds of T.sinskajae UA0300224 and T.astivum PI619379 were less longevous.

scholarly journals Physiological quality of eggplant seeds with different extraction and drying methods

2013 ◽  
Vol 35 (1) ◽  
pp. 51-55 ◽  
Author(s):  
Leomara Vieira de França ◽  
Mariana Dierings Croda ◽  
Warley Marcos Nascimento ◽  
Raquel Alves de Freitas
Keyword(s):  
Hydrochloric Acid ◽  
Seed Quality ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Drying Methods ◽  
Seed Moisture Content ◽  
Seed Moisture ◽  
Seed Extraction ◽  
Physiological Quality

During seed extraction in fleshy fruits, some procedures are necessary to ensure seed quality and minimize deterioration and microorganism activity; also seeds extracted under moist conditions need special care when drying. The objective of this study was to evaluate the physiological quality of eggplant seeds submitted to different extraction and drying methods. In the first study, whole eggplant fruits were mechanically crushed and then fermented for 0, 24 and 48 hours, with and without applying hydrochloric acid during seed extraction. In the second study, seeds were extracted in a pepper peeler and then immediately washed in water and submitted to the following drying methods: Sun/24 h; Sun/48 h; 32 °C/24 h; 32 °C/48 h; 38 °C/24 h; 38 °C/48 h; Sun/24 h + 32 °C/24 h; Sun/24 h + 38 °C/24 h e 32 °C/24 h + 38 °C/24 h. The treatment efficiency of both experiments was evaluated from the following tests: seed weight, germination, first count, accelerated aging and seedling emergence. The results suggest that fermentation and applying hydrochloric acid to pulp/seed reduce the physiological seed quality. All drying methods reduced the seed moisture content permitting adequate storage and maintenance of seed physiological quality.

scholarly journals Accelerated Aging for the Evaluation of the Physiological Potential of Eggplant Seeds

2018 ◽  
Vol 10 (8) ◽  
pp. 477
Author(s):  
Cristiane Deuner ◽  
D. B. Rodrigues ◽  
L. V. M. De Tunes ◽  
A. S. Almeida ◽  
A. S. Suñe ◽  
...  
Keyword(s):  
Moisture Content ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Saturated Solution ◽  
Germination Test ◽  
Randomized Design ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Completely Randomized Design ◽  
Different Levels

The use of healthy and vigorous seeds is necessary to ensure adequate plant stands, therefore, vigor tests are used to measure the physiological potential of seeds alongside with the germination test. The objective of this study was to evaluate different procedures for the estimation of the physiological potential of eggplant seeds through the accelerated aging test. Seed lots were tested for moisture content, germination, first count of germination, seedling emergence and speed of emergence index. Then, seeds were subjected to the conventional accelerated aging test, using an unsaturated NaCl solution or a NaCl saturated solution, for 24, 48 and 72 hours at 41 °C, estimating the moisture content after each period of aging. The treatments were allocated in a completely randomized design, with four treatments (seed lots) and four replicates. The use of the saturated solution of NaCl decreases the absorption of water and the rate of deterioration of eggplant seeds during the accelerated aging test, providing more uniform results. The accelerated aging test allows eggplant seed lots from cv. Napoli to be ranked at different levels of vigor and, among the procedures tested, the combinations 41 °C/48 hours and 41 °C/72 hours allowed the proper assessment of the physiological potential of seeds of this cultivar.

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