MICROBIAL ACTIVITY OF DARK RED LATOSOL SAMPLES STORED AT DIFFERENT TEMPERATURES

The enzymatic activity of soil samples stored at temperatures of 5 to -12oC and at room temperature for 0-32 weeks was determined. While alkaline phosphatase and dehydrogenase activity was decreased compared to control in samples stored at low temperatures, acid phosphatase activity showed no significant change.

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The changes of enzymatic activity of soil under eastern galega (Galega orientalis Lam.) after NPKCa fertilization

Plant Soil and Environment ◽

10.17221/905/2013-pse ◽

2014 ◽

Vol 60 (No. 3) ◽

pp. 123-128 ◽

Cited By ~ 2

Author(s):

B. Symanowicz ◽

S. Kalesa ◽

W. Skorupka ◽

M. Niedbała

Keyword(s):

Alkaline Phosphatase ◽

Acid Phosphatase ◽

Enzymatic Activity ◽

Field Experiment ◽

Soil Samples ◽

Mineral Fertilization ◽

Legume Plant ◽

Ap Horizon ◽

Galega Orientalis ◽

Enzymatic Activity Of Soil

Changes in the enzymatic activity of soil variably fertilized with NPKCa were investigated in a field experiment carried out in 2005–2010. The study was conducted with a legume plant, i.e. eastern galega (Galega orientalis Lam.). The experiment was completely randomized and carried out in three replications with the following mineral fertilization: control, N, P, K, NPK, NP, NK, PK, NPKCa, PKCa, Ca, NKCa, and NPCa. Mineral fertilization was applied in kg/ha: (N-20, P-50, K-160, Ca-150). The soil samples collected from the Ap horizon (0–30 cm) of the rhizosphere in spring after the 1st, 2nd and 3rd swathing had a pHKCl in range from 6.55 to 6.93. The activity of acid phosphatase and alkaline phosphatase was at a low level. The highest activity of urease was recorded in the soil fertilized with NPK, whereas the highest activity of dehydrogenases was in the soil fertilized with PKCa.

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The effect of different copper doses and organic fertilisation on soil’s enzymatic activity

Plant Soil and Environment ◽

10.17221/671/2019-pse ◽

2020 ◽

Vol 66 (No. 2) ◽

pp. 93-98

Author(s):

Beata Kuziemska ◽

Andrzej Wysokiński ◽

Joanna Trębicka

Keyword(s):

Alkaline Phosphatase ◽

Acid Phosphatase ◽

Enzymatic Activity ◽

Phosphatase Activity ◽

Acid Phosphatase Activity ◽

Organic Materials ◽

Chicken Manure ◽

Test Plant ◽

Orchard Grass ◽

Organic Fertilisation

A three-year pot experiment carried out in the vegetation hall in 2014–2016 included studying the enzymatic activity of soil, into which various amounts of copper: (100, 200 and 300 mg Cu/kg soil) and organic materials (cattle manure, chicken manure, post-mushroom substrate) were introduced, used separately, at a soil-introduction dose of 2 g Corg/kg. Copper and organic materials were used once, only in the first year of the study, before sowing test plant orchard grass. In soil collected after the last (fourth) swath of grass in each year of the study, the activity of urease, dehydrogenases, acid, and alkaline phosphatase was determined. Applications of copper to the soil, regardless of its dose, resulted in a decrease in urease, dehydrogenases and alkaline phosphatase and an increase in acid phosphatase activity. The inactivating effect of this metal on the activity of urease, dehydrogenases and alkaline phosphatase increased with the increase of its dose. Organic fertilisation generally increased the enzymatic activity of the analysed soil. In subsequent years of the study, urease and alkaline phosphatase activity decreased, while acid phosphatase activity increased. Dehydrogenase activity did not change significantly in subsequent years of the study.

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STUDIES ON THE MECHANISM OF NIDATION. XVII. HISTOCHEMICAL ANALYSIS OF DECIDUALIZATION IN THE RAT

Acta Endocrinologica ◽

10.1530/acta.0.0500537 ◽

1965 ◽

Vol 50 (4) ◽

pp. 537-559 ◽

Cited By ~ 8

Author(s):

Bertha L. Lobel ◽

Liliane Tic ◽

M. C. Shelesnyak

Keyword(s):

Alkaline Phosphatase ◽

Acid Phosphatase ◽

Connective Tissue ◽

Enzymatic Activity ◽

Acid Phosphatase Activity ◽

Great Variability ◽

Time Intervals ◽

Histochemical Analysis ◽

Metrial Gland ◽

Histochemical Changes

ABSTRACT Histochemical changes in the activities of acid phosphatase, considered to be characteristic of lysosomes, and of alkaline and adenosinetriphosphatase, were studied during the period of regression of deciduomata in the uterus of the pseudopregnant rat. The activity of alkaline phosphatase and adenosinetriphosphatase declined rapidly prior to and during the period of involution. A rise in the activity of acid phosphatase began antimesometrially at the periphery of the deciduomata, before shrinkage was evident in the histological preparations. Acid phosphatase activity remained high during the period of regression and subsequently declined. Enzymatic activity was also high in the metrial gland cells, and in the macrophages which appeared in the uterus and apparently contributed to the breakdown and removal of the deciduomata. Changes were also demonstrated in the amount of free lipids and connective tissue components of the uterus. Great variability was noted in the appearance of uteri from animals killed at the same time intervals following stimulation during the period of regression of deciduomata and this variability was related to the time of occurrence of the first ovulation terminating the period of pseudopregnancy in deciduomata bearing rats.

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Soil response to chemicals used in a field experiment

International Agrophysics ◽

10.2478/v10247-012-0080-0 ◽

2013 ◽

Vol 27 (2) ◽

pp. 151-158 ◽

Cited By ~ 6

Author(s):

S. Jezierska-Tys ◽

A. Rutkowska

Keyword(s):

Alkaline Phosphatase ◽

Enzymatic Activity ◽

Field Experiment ◽

Soil Microorganisms ◽

Block Design ◽

Soil Samples ◽

Alkaline Phosphatases ◽

Soil Response ◽

Negative Effect ◽

Set Up

Abstract The effect of chemicals (Reglone 200 SL and Elastiq 550 EC) on soil microorganisms and their enzymatic activity was estimated. The study was conducted in a field experiment which was set up in the split-block design and comprised three treatments. Soil samples were taken six times, twice in each year of study. The results showed that the application of chemicals generally had no negative effect on the number of soil microorganisms. The application of Reglone 200 SL caused an increase of proteolytic and ureolytic activity and affected the activity of dehydrogenases, acid and alkaline phosphatases in the soil. The soil subjected of Elastiq 550 EC was characterized by lower activity of dehydrogenases, protease, urease and alkaline phosphatase.

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Acid phosphatase, alkaline phosphatase, and nitrate reductase activity of selected ectomycorrhizal fungi

Canadian Journal of Botany ◽

10.1139/b89-101 ◽

1989 ◽

Vol 67 (3) ◽

pp. 750-753 ◽

Cited By ~ 19

Author(s):

Iwan Ho

Keyword(s):

Alkaline Phosphatase ◽

Enzyme Activity ◽

Acid Phosphatase ◽

Nitrate Reductase ◽

Phosphatase Activity ◽

Ectomycorrhizal Fungi ◽

Acid Phosphatase Activity ◽

Nitrate Reductase Activity ◽

Reductase Activity ◽

Phosphatase Alkaline

Seventeen isolates, encompassing five genera and eight species of ectomycorrhizal fungi, were compared for acid phosphatase, alkaline phosphatase, and nitrate reductase activity. Isolates within species differed in enzyme activity and isozyme patterns by host specificity and site (as exemplified by the genus Suillus). Host and site may have affected phosphatase enzyme activity. Generally, the Douglas-fir associates, which dominate in mesic sites, have higher acid phosphatase activity than pine associates, which mostly occupy xeric sites; however, pine associates from mesic sites also have higher acid phosphatase activity (e.g., S. tomentosus). In four isolates of Amanita muscaria, the effect of site was also apparent. Two of them, which have significantly higher acid phosphatase activity than the others, were isolated from mesic sites. The isozyme pattern of the genus Suillus appeared to be separated by host groups. Other isolates with only one species also differed more or less by host groups. They shared at least one band within host groups, except for the two isolates of Paxillus involutus from different hosts. The P. involutus S-403 isolated from an orchard showed much higher nitrate reductase activity than all other isolates. No apparent differences in nitrate reductase activity were found between the other isolates.

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Phosphatase Activity of Drosophila Salivary Glands

Journal of Cell Science ◽

10.1242/jcs.s3-89.8.415 ◽

1948 ◽

Vol s3-89 (8) ◽

pp. 415-419

Author(s):

W. L. DOYLE

Keyword(s):

Acid Phosphatase ◽

Enzymatic Activity ◽

Quantitative Determination ◽

Salivary Glands ◽

Phosphatase Activity ◽

Acid Phosphatase Activity ◽

The State ◽

Staining Methods

The phosphatases in the cytoplasm and nuclei of Drosophila salivary glands are better preserved by fixation in absolute acetone than in 85 per cent, alcohol. In whole glands there is relatively little extraction of the enzyme during assay. Phosphatase activity is more resistant to incubation at neutrality than at pH 8.6, but in this material there is sufficient residual enzymatic activity to permit redetermination of alkaline, neutral, or acid phosphatase activity by staining methods after an initial quantitative determination. The state of the membranes of the gland affects the penetration of the substrate sufficiently to limit the activities obtained.

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Acid and alkaline phosphatases in the lymphomyeloid tissues of elasmobranch fish

Journal of the Marine Biological Association of the United Kingdom ◽

10.1017/s0025315400041370 ◽

1978 ◽

Vol 58 (3) ◽

pp. 727-730 ◽

Cited By ~ 4

Author(s):

Ragnar Fänge

Keyword(s):

Alkaline Phosphatase ◽

Acid Phosphatase ◽

High Activity ◽

Phosphatase Activity ◽

Acid Phosphatase Activity ◽

Alkaline Phosphatases ◽

Large Numbers ◽

Alkaline Reaction ◽

Elasmobranch Fish ◽

Very High

Activities of phosphomonoesterases were measured at acid and at alkaline reaction (pH 4–5 or 9–65) in homogenates of elasmobranch tissues especially lymphomyeloid structures. The animals were dogfish (Scyliorhinus caniculd) and two species of ray (Raja brachyura, R. naevus). Acid phosphatase activity was high in the epigonal tissue, Leydig's organ, the spleen and the thymus. High activity was also found in the pancreas and the kidney, whereas skeletal and cardiac muscle showed low values. The activity of alkaline phosphatase was very high in the kidney and relatively low in other tissues. Ultrasonification of homogenates from the dogfish resulted in increase of acid phosphatase activity but had little effect on alkaline phosphatase activity. The high activity of acid phosphatase in lymphomyeloid tissue may be due to the presence of large numbers of various types of leucocytes.

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Unbeeinflußbarkeit ihrer Wirkung auf Ribonuclease durch Cystamin und tiefe Temperaturen

Zeitschrift für Naturforschung B ◽

10.1515/znb-1966-1213 ◽

1966 ◽

Vol 21 (12) ◽

pp. 1165-1170 ◽

Cited By ~ 3

Author(s):

H. Jung

Keyword(s):

Cross Section ◽

Low Temperatures ◽

Room Temperature ◽

High Efficiency ◽

Reduction Factor ◽

Biological Damage ◽

Nuclear Collisions ◽

Different Temperatures ◽

Fast Protons ◽

Mev Protons

Slow protons having energies below 1.5 keV dissipate their kinetic energy in matter through elastic nuclear collisions. By this process atoms are displaced out from their original positions in macromolecules. This was recently shown to cause biological damage with high efficiency. Experiments are described to test the possibility of modifying the sensitivity of ribonuclease towards elastic collisions by protective agents and by low temperatures. When cystamine is present during irradiation dry ribonuclease is protected against the action of “ionizing” fast protons (2 MeV), the dose reduction factor being 1.8. But no protection is observed when inactivation is achieved by elastic nuclear collisions (proton energy 1 keV and 1.4 keV). Similar results were obtained when the irradiations were carried out at different temperatures. Using 2 MeV protons the radiosensitivity of ribonuclease was found to be 3 times higher at room temperature than at 125 °K, but when using slow protons of 1.4 keV energy the inactivation cross section turned out to be independent of temperature. This shows that the action of elastic nuclear collisions can be modified neither by cystamine nor by low temperatures.

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