scholarly journals Effects of FGF-2 and EGF removal on the differentiationof mouse neural precursor cells

2009 ◽  
Vol 81 (3) ◽  
pp. 443-452 ◽  
Author(s):  
Telma T. Schwindt ◽  
Fabiana L. Motta ◽  
Barnabé Gabriela F. ◽  
Massant Cristina G. ◽  
Alessander O. Guimarães ◽  
...  
Keyword(s):  
Growth Factors ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
Control Groups ◽  
Endogenous Production ◽  
Wide Range ◽  
And Control ◽  
The Ideal

Cell therapy for neurological disorders has advanced, and neural precursor cells (NPC) may become the ideal candidates for neural transplantation in a wide range of diseases. However, additional work has to be done to determine either the ideal culture environment for NPC expansion in vitro, without altering their plasticity, or the FGF-2 and EGF mechanisms of cell signaling in neurospheres growth, survival and differentiation. In this work we evaluated mouse neurospheres cultured with and without FGF-2 and EGF containing medium and showed that those growth factors are responsible for NPC proliferation. It is also demonstrated that endogenous production of growth factors shifts from FGF-2 to IGF-1/PDGFb upon EGF and FGF-2 withdrawal. Mouse NPC cultured in suspension showed different patterns of neuronal localization (core versus shell) for both EGF and FGF-2 withdrawal and control groups. Taken together, these results show that EGF and FGF-2 removal play an important role in NPC differentiation and may contribute to a better understanding of mechanisms of NPC differentiation. Our findings suggest that depriving NPC of growth factors prior to grafting might enhance their chance to effectively integrate into the host.

In vitro differentiation of human bone marrow stromal cells into neural precursor cells using small molecules

2021 ◽  
Vol 363 ◽  
pp. 109340
Author(s):  
Abeer Sallam ◽  
Thangirala Sudha ◽  
Noureldien H.E. Darwish ◽  
Samar Eghotny ◽  
Abeer E-Dief ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Small Molecules ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Precursor Cells ◽  
Marrow Stromal Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
In Vitro Differentiation

scholarly journals Molecular Mechanism of Systemic Delivery of Neural Precursor Cells to the Brain: Assembly of Brain Endothelial Apical Cups and Control of Transmigration by CD44

Stem Cells ◽  
2008 ◽  
Vol 26 (7) ◽  
pp. 1673-1682 ◽  
Author(s):  
Christine Rampon ◽  
Nicolas Weiss ◽  
Cyrille Deboux ◽  
Nathalie Chaverot ◽  
Florence Miller ◽  
...  
Keyword(s):  
Molecular Mechanism ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
Systemic Delivery ◽  
And Control ◽  
The Brain

Adult human spinal cord harbors neural precursor cells that generate neurons and glial cells in vitro

2008 ◽  
Vol 86 (9) ◽  
pp. 1916-1926 ◽  
Author(s):  
C. Dromard ◽  
H. Guillon ◽  
V. Rigau ◽  
C. Ripoll ◽  
J.C. Sabourin ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Glial Cells ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
Human Spinal Cord ◽  
Adult Human

Comparison of the therapeutic potential of adult and embryonic neural precursor cells in a rat model of Parkinson disease

2008 ◽  
Vol 108 (1) ◽  
pp. 149-159 ◽  
Author(s):  
Kenichiro Muraoka ◽  
Tetsuro Shingo ◽  
Takao Yasuhara ◽  
Masahiro Kameda ◽  
Wen Ji Yuen ◽  
...  
Keyword(s):  
Parkinson Disease ◽  
Rat Model ◽  
Fluorescent Protein ◽  
Therapeutic Potential ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
Therapeutic Effects ◽  
Adenoviral Infection

Object The therapeutic effects of adult and embryonic neural precursor cells (NPCs) were evaluated and their therapeutic potential compared in a rat model of Parkinson disease. Methods Adult NPCs were obtained from the subventricular zone and embryonic NPCs were taken from the ganglionic eminence of 14-day-old embryos. Each NPC type was cultured with epidermal growth factor. The in vitro neuronal differentiation rate of adult NPCs was approximately equivalent to that of embryonic NPCs after two passages. Next, the NPCs were transfected with either green fluorescent protein or glial cell line–derived neurotrophic factor (GDNF) by adenoviral infection and transplanted into the striata in a rat model of Parkinson disease (PD) induced by unilateral intrastriatal injection of 6-hydroxydopamine. An amphetamine-induced rotation test was used to evaluate rat behavioral improvement, and immunohistochemical analysis was performed to compare grafted cell survival, differentiation, and host tissue changes. Results The rats with GDNF-transfected NPCs had significantly fewer amphetamine-induced rotations and less histological damage. Except for the proportion of surviving grafted cells, there were no significant differences between adult and embryonic NPCs. Conclusions Adult and embryonic NPCs have a comparable therapeutic potential in a rat model of PD.

scholarly journals Prolonged cultivation of hippocampal neural precursor cells shifts their differentiation potential and selects for aneuploid cells

2013 ◽  
Vol 394 (12) ◽  
pp. 1623-1636 ◽  
Author(s):  
The Duy Nguyen ◽  
Darius Widera ◽  
Johannes Greiner ◽  
Janine Müller ◽  
Ina Martin ◽  
...  
Keyword(s):  
Cell Types ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
Early Passage ◽  
Differentiation Potential ◽  
Developmental Potential ◽  
Prolonged Cultivation ◽  
Tumorigenic Transformation

Abstract Neural precursor cells (NPCs) are lineage-restricted neural stem cells with limited self-renewal, giving rise to a broad range of neural cell types such as neurons, astrocytes, and oligodendrocytes. Despite this developmental potential, the differentiation capacity of NPCs has been controversially discussed concerning the trespassing lineage boundaries, for instance resulting in hematopoietic competence. Assessing their in vitro plasticity, we isolated nestin+/Sox2+, NPCs from the adult murine hippocampus. In vitro-expanded adult NPCs were able to form neurospheres, self-renew, and differentiate into neuronal, astrocytic, and oligodendrocytic cells. Although NPCs cultivated in early passage efficiently gave rise to neuronal cells in a directed differentiation assay, extensively cultivated NPCs revealed reduced potential for ectodermal differentiation. We further observed successful differentiation of long-term cultured NPCs into osteogenic and adipogenic cell types, suggesting that NPCs underwent a fate switch during culture. NPCs cultivated for more than 12 passages were aneuploid (abnormal chromosome numbers such as 70 chromosomes). Furthermore, they showed growth factor-independent proliferation, a hallmark of tumorigenic transformation. In conclusion, our findings substantiate the lineage restriction of NPCs from adult mammalian hippocampus. Prolonged cultivation results, however, in enhanced differentiation potential, which may be attributed to transformation events leading to aneuploid cells.

scholarly journals Conserved fate and function of ferumoxides-labeled neural precursor cells in vitro and in vivo

2009 ◽  
pp. NA-NA ◽  
Author(s):  
Mikhal E. Cohen ◽  
Naser Muja ◽  
Nina Fainstein ◽  
Jeff W.M. Bulte ◽  
Tamir Ben-Hur
Keyword(s):  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
And Function

scholarly journals Reconstruction of neural tube-like structures in vitro from primary neural precursor cells.

1993 ◽  
Vol 90 (20) ◽  
pp. 9683-9687 ◽  
Author(s):  
Y. Tomooka ◽  
H. Kitani ◽  
N. Jing ◽  
M. Matsushima ◽  
T. Sakakura
Keyword(s):  
Neural Tube ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor

scholarly journals Proneural bHLH neurogenin 2 differentially regulates Nurr1-induced dopamine neuron differentiation in rat and mouse neural precursor cells in vitro

FEBS Letters ◽  
2008 ◽  
Vol 582 (5) ◽  
pp. 537-542 ◽  
Author(s):  
Chang-Hwan Park ◽  
Jin Sun Kang ◽  
Eun-Hye Yoon ◽  
Jae-Won Shim ◽  
Haeyoung Suh-Kim ◽  
...  
Keyword(s):  
Precursor Cells ◽  
Dopamine Neuron ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
Neuron Differentiation
Sign in / Sign up

Export Citation Format

Share Document