scholarly journals Evaluation of analgesic and anti-inflammatory activities of Hydrocotyle umbellata L., Araliaceae (acariçoba) in mice

2013 ◽  
Vol 85 (3) ◽  
pp. 987-997 ◽  
Author(s):  
IZIARA F. FLORENTINO ◽  
MARCUS VINICIUS M. NASCIMENTO ◽  
PABLINNY M. GALDINO ◽  
ADRIANE F. DE BRITO ◽  
FABIO F. DA ROCHA ◽  
...  
Keyword(s):  
Formalin Test ◽  
Pleural Cavity ◽  
Folk Medicine ◽  
Ethanolic Extract ◽  
Nociceptive Response ◽  
Plasma Extravasation ◽  
Hot Plate Test ◽  
Writhing Test ◽  
Skin Ulcers ◽  
Anti Inflammatory

The Hydrocotyle umbellata L. is a specimen of the Araliaceae family popularly known as acariçoba. Its indications in folk medicine include treatment of skin ulcers, and rheumatism. The aim of this study was to evaluate the antinociceptive and anti-inflammatory activities of the ethanolic extract from acariçoba's underground parts (EEA). EEA reduced the nociceptive response of the animals as evaluated in the acetic acid-induced writhing test and in both phases of formalin test. EEA also presented a supraspinal analgesic activity by increasing the pain latency in the hot plate test. Moreover, EEA reduced the leukocytes migration and plasma extravasation to pleural cavity in the carrageenan-induced pleurisy, besides reducing the edema induced by carrageenan until the second hour and also the edema induced by dextran. In conclusion our results showed that EEA of H. umbellata L. presents analgesic and anti-inflammatory activities, and that a blockade of activity or reduction in the release of different mediators, such as histamine and serotonin, could be involved in these pharmacologic effects.

scholarly journals Antinociceptive and Toxicological Effects ofDioclea grandifloraSeed Pod in Mice

2010 ◽  
Vol 2010 ◽  
pp. 1-6 ◽  
Author(s):  
Rita de Cássia da Silveira e Sá ◽  
Leandra Eugênia Gomes de Oliveira ◽  
Franklin Ferreira de Farias Nóbrega ◽  
Jnanabrata Bhattacharyya ◽  
Reinaldo Nóbrega de Almeida
Keyword(s):  
Motor Performance ◽  
Formalin Test ◽  
Antinociceptive Effect ◽  
Intraperitoneal Administration ◽  
Ethanolic Extract ◽  
Neurogenic Pain ◽  
Hot Plate Test ◽  
Writhing Test ◽  
Toxicological Effects ◽  
Observation Period

The acute treatment of mice with an ethanolic extract from the seed pod ofDioclea grandiflora(EDgP) at doses of 75, 150 and 300 mg/kg by intraperitoneal administration produced a significant antinociceptive effect as displayed by the acetic acid-induced writhing test and the formalin test. The antinociception was observed through the first (neurogenic pain) and second (inflammatory pain) phases in the formalin test. The hot plate test did not show an increase in the antinociceptive latency whereas the motor performance was affected by the administration at 300 mg/kg at the beginning (30 minutes) of the observation period but not at later periods (60 and 120 minutes). These results suggest that EDgP has a central antinociceptive action and a possible anti-inflammatory activity in mice.

scholarly journals The Antinociceptive and Anti-Inflammatory Activities ofAspidosperma tomentosum(Apocynaceae)

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Anansa Bezerra de Aquino ◽  
Luiz Henrique Agra Cavalcante-Silva ◽  
Carolina Barbosa Brito da Matta ◽  
Willians Antônio do Nascimento Epifânio ◽  
Pedro Gregório Vieira Aquino ◽  
...  
Keyword(s):  
Soluble Fraction ◽  
Ethanolic Extract ◽  
Opioid Antagonist ◽  
Hot Plate ◽  
Hot Plate Test ◽  
Writhing Test ◽  
Plate Test ◽  
Inflammatory Phase ◽  
Soluble Phase ◽  
Anti Inflammatory

We investigated the antinociceptive and anti-inflammatory activities of the crude ethanolic extract (CEE), its fractions, and the flavonoid isorhamnetin fromAspidosperma tomentosumusing models of nociception and inflammation in mice. In the writhing test, the CEE and its fractions (except for soluble phase, CHCl3100% and EtAcO 100%) at 100 mg/kg p.o. induced antinociceptive activity. Isorhamnetin (100 μmol/kg, p.o.) was also active. In the hot plate test, only the treatment with the fractions Hex : CHCl350%, CHCl3100%, and CHCl3 : MeOH 5% (100 mg/kg, p.o.) increased the latency time, reversed by the opioid antagonist naloxone. Fractions that were active in the hot plate test did not show catalepsy condition. It was observed that CEE, all fractions, and isorhamnetin reduced the formalin effects in the neurogenic phase. In the inflammatory phase, only CEE, isorhamnetin, and CHCl3100% and CHCl3 : MeOH 5% fractions were active. CEE and all fractions, except for CHCl3 : MeOH 10% fraction, isorhamnetin, and soluble fraction were able to produce an antioedematogenic activity in the ear capsaicin-induced edema test. In the thioglycolate-induced peritonitis, only EtAcO 100% fraction was not active. The results demonstrate thatA. tomentosumhas antinociceptive and anti-inflammatory activities in animal models.

scholarly journals Antinociceptive and anti-inflammatory effects of a Geissospermum vellosii stem bark fraction

2016 ◽  
Vol 88 (1) ◽  
pp. 237-248 ◽  
Author(s):  
JOSÉLIA A. LIMA ◽  
THIAGO W.R. COSTA ◽  
LEANDRO L. SILVA ◽  
ANA LUÍSA P. MIRANDA ◽  
ANGELO C. PINTO
Keyword(s):  
Formalin Test ◽  
Ethanolic Extract ◽  
Stem Bark ◽  
Second Phase ◽  
Murine Models ◽  
Hot Plate Test ◽  
Rat Paw Edema ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Abdominal Constriction

ABSTRACT Geissospermum vellosii (Pao pereira) is a Brazilian tree whose stem barks are rich in indole alkaloids that present intense anticholinesterase activity. The present study evaluated the effects of a stem bark fraction (PPAC fraction) and ethanolic extract (EE) of Pao pereira in classic murine models of inflammation and pain. The EE and PPAC fraction, both at a dose of 30 mg/kg, significantly reduced mice abdominal constriction induced by acetic acid by 34.8% and 47.5%, respectively. In the formalin test, EE (30 mg/kg) and PPAC fraction (30 and 60 mg/kg) inhibited only the second phase, by 82.8%, 84.9% and 100%, respectively. Compared with indomethacin, similar doses of EE or PPAC fraction were approximately twice as effective in causing antinociception. PPAC fraction was not effective in the hot plate test but reduced the inflammatory response at the second (50.6%) and third (57.8%) hours of rat paw edema induced by carrageenan. Antihyperalgesic activity was observed within 30 min with a peak at 2 h (60.1%). These results demonstrate that compounds in PPAC fraction have anti-inflammatory and antinociceptive activity by a mechanism apparently unrelated to the opioid system. Regardless of similar responses to indomethacin, the effects of PPAC fraction are mainly attributed to acetylcholine actions.

scholarly journals Phytochemical Analysis, Antioxidant and Anti-Inflammatory Activity of Calyces from Physalis peruviana

2014 ◽  
Vol 9 (11) ◽  
pp. 1934578X1400901 ◽  
Author(s):  
Reina M. Toro ◽  
Diana M. Aragón ◽  
Luis F. Ospina ◽  
Freddy A. Ramos ◽  
Leonardo Castellanos
Keyword(s):  
Antioxidant Activities ◽  
Folk Medicine ◽  
Ethanolic Extract ◽  
Inflammatory Activity ◽  
Phytochemical Analysis ◽  
Physalis Peruviana ◽  
Edema Model ◽  
Anti Inflammatory ◽  
Bio Assay

Physalis peruviana calyces are used extensively in folk medicine. The crude ethanolic extract and some fractions of calyces were evaluated in order to explore antioxidant and anti-inflammatory activities. The anti-inflammatory activity was evaluated by the TPA-induced ear edema model. The antioxidant in vitro activity was measured by means of the superoxide and nitric oxide scavenging activity of the extracts and fractions. The butanolic fraction was found to be promising due to its anti-inflammatory and antioxidant activities. Therefore, a bio-assay guided approach was employed to isolate and identify rutin (1) and nicotoflorin (2) from their NMR spectroscopic and MS data. The identification of rutin in calyces of P. peruviana supports the possible use of this waste material for phytotherapeutic, nutraceutical and cosmetic preparations.

scholarly journals Anti-Inflammatory, Analgesic and Antipyretic Activity of Catunaregam spinosa (Thumb.) Tirveng Extracts

2019 ◽  
Vol 9 (5) ◽  
pp. 89-94
Author(s):  
Hansa Saini ◽  
Jayesh Dwivedi ◽  
Himanshu Paliwal ◽  
Udichi Kataria ◽  
Priyanka Chauhan ◽  
...  
Keyword(s):  
Ethanolic Extract ◽  
Pharmacological Properties ◽  
Phytochemical Screening ◽  
Hot Plate ◽  
Hot Plate Test ◽  
Egg Albumin ◽  
Antipyretic Activity ◽  
Ethanolic Extracts ◽  
Anti Inflammatory ◽  
Response Method

Catunaregam spinosa leaves have been ethnopharmacologically accounted for acquiring various pharmacological properties. The present study was undertaken to evaluate anti-inflammatory, analgesic and antipyretic potential of leaves of C. spinosa.The ethanolic extract was selected for this purpose based on phytochemical screening. Inflammation was inhibited at the dose of 200 mg/kg with percent inhibition of inflammation 32.06, 37.28 and 43.16 %, respectively, at 1, 3 and 5 h, while in egg albumin model % inhibition was found to be 47.81%. There was no significant analgesic activity seen in acetic acid induced writhing response method while significant effects were observed in the doses of 25 and 100 mg/kg on hot plate test. No antipyretic activity was shown by ethanolic extracts(25, 100 and 200 mg/kg) against Brewer’s yeast induced pyrexia in rats. Keywords: Catunaregam spinosa, Anti-inflammatory activity, Phytochemical screening, Ethanolic extract.

scholarly journals Evaluation of antinociceptive and anti-inflammatory activities of the topical preparation of Cipura paludosa (Iridaceae)

2014 ◽  
Vol 44 (2) ◽  
pp. 263-270 ◽  
Author(s):  
José Antônio Paixão da Silva Neto ◽  
Larissa Dias Menezes ◽  
Gúbio Oliveira Gomes ◽  
Elise Marques Freire Cunha ◽  
Mariângela Soares de Azevedo ◽  
...  
Keyword(s):  
Statistical Difference ◽  
Formalin Test ◽  
Late Phase ◽  
Ethanolic Extract ◽  
Control Group ◽  
Paw Edema ◽  
Antinociceptive Action ◽  
Amazon Rain Forest ◽  
Accelerated Stability ◽  
Anti Inflammatory

Alho do mato (Cipura paludosa, Iridaceae) is a medicinal plant found in the Amazon rain forest, North of Brazil. It has been used to treat algic, inflammatory and infectious processes. The aim of this study was to evaluate the anti-inflammatory and antinociceptive action of the crude Cipura paludosa ethanolic extract at concentrations ranging between 2.0 and 4.0% in Oil and Water cream formulations for topical use. The physical-chemical stability of the formulations was monitored over a six-month period with the use of accelerated stability tests. In order to evaluate the anti-inflammatory and antinociceptive activities, we used a paw edema test induced by carrageenan and a formalin test, respectively. The paw edema test showed that there was a statistical difference in the control group in relation to the treatments. The formalin test did not confirm antinociceptive action of the treatments with the extract in the early phase of the test. However, statistical difference was confirmed for the treatments in relation to the control in the late phase. The antinociceptive and anti-inflammatory activities of Cipura paludosa preparations, as demonstrated in the results, at least partially support the ethno-medical uses of this plant.

scholarly journals Effect of Rosmarinic and Caffeic Acids on Inflammatory and Nociception Process in Rats

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Giovana Duzzo Gamaro ◽  
Edna Suyenaga ◽  
Milene Borsoi ◽  
Joice Lermen ◽  
Patrícia Pereira ◽  
...  
Keyword(s):  
Caffeic Acid ◽  
Rosmarinic Acid ◽  
Pleural Cavity ◽  
Biological Activities ◽  
Control Group ◽  
Tail Flick ◽  
Nociceptive Response ◽  
Tail Flick Latency ◽  
Balanced Distribution ◽  
Anti Inflammatory

Rosmarinic acid is commonly found in species of the Boraginaceae and the subfamily Nepetoideae (Lamiaceae). It has a number of interesting biological activities, for example, antiviral, antibacterial, anti-inflammatory, and antioxidant. The aim of the present study was to investigate the effect of the i.p. administration of caffeic and rosmarinic acid (5 and 10 mg/kg) on anti-inflammatory and nociceptive response using carrageenan-induced pleurisy model and tail-flick assay in rats. The analysis of cells in the pleural exudates revealed a reduction of 66% of the number of leukocytes that migrated to the pleural cavity in the animals treated with 5 mg/kg caffeic acid, and of 92.9% for the animals treated with 10 mg/kg in comparison with the control group. These exudates showed a balanced distribution of polymorphonuclear (PMN) and mononuclear (MN) cells, differently from the control group, in which PMN cells were predominant. The analysis to tail-flick latency was increased in the group treated with 10 mg/kg caffeic acid characterizing a nociceptive response. While there was no difference between control group and animals treated with rosmarinic.

scholarly journals Antinociceptive and anti-inflammatory activities of Hymenaea martiana Hayne (Fabaceae) in mice

2022 ◽  
Vol 82 ◽  
Author(s):  
A. G. M. Pacheco ◽  
E. J. Pacheco ◽  
L. A. R. O. Macedo ◽  
J. C. Silva ◽  
S. R. G. Lima-Saraiva ◽  
...  
Keyword(s):  
Folk Medicine ◽  
Ethanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Leukocyte Migration ◽  
Northeastern Brazil ◽  
Hot Plate ◽  
Ms Analysis ◽  
Anti Inflammatory ◽  
Inflammatory Profile ◽  
Thermal Stimuli

Abstract Hymenaea martiana is a species popularly known in Northeastern Brazil as “jatobá” and used in folk medicine to treat pain and inflammation. The aim of this work was to evaluate the antinociceptive and anti-inflammatory activity of H. martiana. In the present study, we carried out an investigation about the effects of the crude ethanolic extract (Hm-EtOH) and the ethyl acetate fraction (Hm-AcOEt) in models of nociception and inflammation in mice. Chemical (acetic acid-induced writhing and formalin) and thermal stimuli (hot plate) were used for the evaluation of antinociceptive activity, while for the anti-inflammatory profile paw edema induced by carrageenan was used, along with leukocyte migration to the peritoneal cavity. The presence of the flavonoid astilbin in the samples was characterized through HPLC-DAD-MS analysis. Hm-EtOH and Hm-AcOEt (100, 200 and 400 mg.kg-1, i.p.) significantly reduced the number of abdominal contortions and decreased the paw licking time in the formalin test. In the hot plate, the extract increased the latency time of animals. Hm-EtOH and Hm-AcOEt inhibited significantly the increase in the edema after the administration of carrageenan. Hm-EtOH and Hm-AcOEt inhibited leukocyte migration in the peritonitis test. HPLC-DAD-MS analysis of Hm-EtOH and Hm-AcOEt revealed the presence of the flavonoid astilbin in the samples. According to the results of this study, both Hm-EtOH and Hm-AcOEt have antinociceptive and anti-inflammatory activities, which could be related with the presence of flavonoid in the extracts. The results reinforce the popular use of this plant.

scholarly journals HPTLC analysis and anti-inflammatory activity of Plectranthus amboinicus (Lour.) leaves

2021 ◽  
Vol 12 (3) ◽  
pp. 2077-2082
Author(s):  
Ananthi T ◽  
Ramya V
Keyword(s):  
Antiinflammatory Activity ◽  
Protein Denaturation ◽  
Folk Medicine ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Metabolite Profile ◽  
Solvent Mixture ◽  
Phytochemical Analysis ◽  
Anti Inflammatory ◽  
Plectranthus Amboinicus

Plectranthus amboinicus (Lour.) Spreng. are generally used in Chinese folk medicine for treating fever, cough, mumps, sore throats, and mosquito bite. The current study was therefore carried out to provide vital pharmacological details about the leaf. This study investigated the anti-inflammatory properties of ethanolic extract from Plectranthus amboinicus. The leaves of Plectranthus amboinicus were rinsed, air dried and then powdered using machine. The ethanol extract of the plant was used for phytochemical analysis to identify the constituents present in the plants. HPTLC analysis was carried out to investigate its secondary metabolite profile by using the solvent mixture. The anti-inflammatory effect was analysed by inhibition of protein denaturation assay. The hypotonicity induced haemolysis was observed from 100µg/ml to 500µg/ml of extract concentration. The results proved that the ethanolic leaf extract of P.amboinicus has antiinflammatory activity. The presence of flavonoid, quercetin in plant extract was confirmed by HPTLC analysis and these flavonoids may be responsible for the antiinflammatory activity. Further studies are needed to the development of potential drug that may be used for various pharmacological activities.

scholarly journals INVESTIGATION OF ANTI-INFLAMMATORY AND ANTINOCICEPTIVE EFFECTS OF AQUEOUS EXTRACTS OF ARTEMISIA AFRA IN WISTAR RATS

2018 ◽  
Vol 11 (12) ◽  
pp. 190 ◽  
Author(s):  
Mavuto Gondwe ◽  
Anda Mpalala ◽  
Lusanda Zongo ◽  
David Kamadyaapa ◽  
Eugene Ndebia ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Biological Effects ◽  
Control Group ◽  
Paw Edema ◽  
Hot Plate ◽  
Traditional Practice ◽  
Hot Plate Test ◽  
Writhing Test ◽  
Antinociceptive Effects ◽  
Anti Inflammatory

Objective: The objective of the study was to evaluate the anti-inflammatory and antinociceptive effects of Artemisia afra.Methods: Animals were randomly divided into five groups of six animals each and administered with normal saline (2 ml/kg), indomethacin (10 mg/ kg), and A. afra at doses of 100, 200, and 400 mg/kg, respectively. For the anti-inflammatory activity, carrageenan-induced paw edema was used while the hot plate and acetic acid induced-writhing tests were used to assess the antinociceptive activity.Results: Pretreatment with A. afra at a dose of 100 mg/kg did not show any significant biological effects (p>0.05) for any of the three tests, when compared against saline-treated control group. At a dose of 200 mg/kg, A. afra demonstrated significant effects (p<0.01), during the 5th h reducing carrageenan-induced paw edema by 12%. The highest dose (400 mg/kg) of A. afra demonstrated more potent effects by decreasing the carrageenan-induced paw swelling (p<0.001–0.05) during the 3rd, 4th, and 5th h, by up to 38% when compared against saline-treated control group. Both the 200 and 400 mg/kg, A. afra doses achieved a significant increase (p<0.05) in reaction time in the hot plate test. In the acetic acid-induced writhing test, pretreatment with A. afra (400 mg/kg) significantly reduced pain by 39% (p<0.01) by comparison with the saline control.Conclusion: Experimental data demonstrated that aqueous extract of A. afra possesses anti-inflammatory and antinociceptive properties in experimental acute inflammation and pain. These findings support the usage of A. afra in managing inflammation and pain in traditional practice.

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