scholarly journals Antinociceptive and anti-inflammatory effects of a Geissospermum vellosii stem bark fraction

2016 ◽  
Vol 88 (1) ◽  
pp. 237-248 ◽  
Author(s):  
JOSÉLIA A. LIMA ◽  
THIAGO W.R. COSTA ◽  
LEANDRO L. SILVA ◽  
ANA LUÍSA P. MIRANDA ◽  
ANGELO C. PINTO
Keyword(s):  
Formalin Test ◽  
Ethanolic Extract ◽  
Stem Bark ◽  
Second Phase ◽  
Murine Models ◽  
Hot Plate Test ◽  
Rat Paw Edema ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Abdominal Constriction

ABSTRACT Geissospermum vellosii (Pao pereira) is a Brazilian tree whose stem barks are rich in indole alkaloids that present intense anticholinesterase activity. The present study evaluated the effects of a stem bark fraction (PPAC fraction) and ethanolic extract (EE) of Pao pereira in classic murine models of inflammation and pain. The EE and PPAC fraction, both at a dose of 30 mg/kg, significantly reduced mice abdominal constriction induced by acetic acid by 34.8% and 47.5%, respectively. In the formalin test, EE (30 mg/kg) and PPAC fraction (30 and 60 mg/kg) inhibited only the second phase, by 82.8%, 84.9% and 100%, respectively. Compared with indomethacin, similar doses of EE or PPAC fraction were approximately twice as effective in causing antinociception. PPAC fraction was not effective in the hot plate test but reduced the inflammatory response at the second (50.6%) and third (57.8%) hours of rat paw edema induced by carrageenan. Antihyperalgesic activity was observed within 30 min with a peak at 2 h (60.1%). These results demonstrate that compounds in PPAC fraction have anti-inflammatory and antinociceptive activity by a mechanism apparently unrelated to the opioid system. Regardless of similar responses to indomethacin, the effects of PPAC fraction are mainly attributed to acetylcholine actions.

scholarly journals Anti-inflammatory Effect of Ampelocissus Indica (L.) Planch (Chembravalli) in Wistar Rats

2020 ◽  
Author(s):  
Nithya V S ◽  
Indulekha V C ◽  
Helen Antony
Keyword(s):  
Acute Toxicity ◽  
Ethanolic Extract ◽  
Chemical Profiling ◽  
Volume Weight ◽  
Rat Paw Edema ◽  
Tnf Α ◽  
Experimental Inflammation ◽  
Phytochemical Profile ◽  
Anti Inflammatory ◽  
Rat Paw

Abstract Background Ampelocissus indica (L.)Planch, Vitaceae locally known as Chembravalli, is medicinal plant used for inflammatory skin ailments and documented in Hortus Malabaricus, and traditional Ayurveda books. Objective To conduct phyto-chemical and acute toxicity of root of A.indica and to evaluate its anti-inflammatory activity. Materials and methods Phyto-chemical profiling of root of A.indica was done as per standard ICMR methods and acute toxicity as per OECD-425. Decoction of A.indica (AI-8.64 ml/kg, AI-4.32 ml/kg) and ethanolic extract (AIE-500 mg/kg) were orally given to rats with Carrageenan induced rat paw edema and also in other rats with Cotton pellet induced granuloma; paw volume, weight of granuloma, histopathology, serum TNF α, IL1β were assessed. Results Oral administration of decoction AI-8.64 ml/kg, AI-4.32 ml/kg, and AIE-500 mg/kg significantly reduced inflammation by reducing paw volume, serum TNF α, IL1β. AI-8.64 ml/kg significantly reduced weight of granuloma and serum TNF α, IL1β. No acute toxicity was found upto 2000 mg/kg. Flavonoids, phenols, tannins, saponins, and steroids found in A.indica extract. Conclusion The present finding suggested that A.indica has a protective effect on experimental inflammation in rats and phytochemical profile will be useful for further studies

Expression of B1 kinin receptors mediating paw edema and Formalin-induced nociception. Modulation by glucocorticoids

1995 ◽  
Vol 73 (7) ◽  
pp. 812-819 ◽  
Author(s):  
Maria M. Campos ◽  
Luciana V. Mata ◽  
João B. Callxto
Keyword(s):  
Formalin Test ◽  
Systemic Treatment ◽  
Second Phase ◽  
Paw Edema ◽  
Systemic Injection ◽  
Kinin Receptors ◽  
Rat Paw Edema ◽  
Inflammatory Processes ◽  
Second Phases ◽  
Rat Paw

Bradykinin (BK) and Tyr8-BK induced graded rat paw edema with EC50 values of 1.9 and 1.1 nmol/paw, while des-Arg9-BK (DABK, up to 300 nmol/paw) was marginally effective. Tyr8-BK, but not DABK, also caused a dose-related increase in mouse paw edema, with an EC50 of 1.3 nmol/paw. The response to Tyr8-BK (10 nmol/paw) in rat paw edema was antagonized by B2 receptor antagonists (HOE-140 or NPC 17731, 30 nmol/paw) but not by the B1 antagonist des-Arg9[Leu8]BK (DALBK, 100 nmol/paw). Daily intraplantar injections of Tyr8-BK (10 nmol/paw) for 7 days caused progressive desensitization (D) of edema in sham-operated and adrenalectomized Wistar rats. DABK (100 nmol/paw) caused marked paw edema in D paws from both groups, which was inhibited by DALBK (100 nmol/paw) and by dexamethasone (0.5 mg/kg, s.c). Systemic injection of lipopolysaccharide (10 μg/mouse, 24 h prior) potentiated the first and second phases of Formalin-induced pain but had no effect on paw edema. Coinjection of DABK (2–22 nmol/paw) with low doses of Formalin in lipopolysaccharide-treated mice, which had no effect on naive animals, dose dependently potentiated both phases of Formalin-induced pain but did not modify paw edema. These effects were antagonized by DALBK with ID50 values of 21.9 (first phase) and 64.1 (second phase) nmol/paw. Thus, both progressive desensitization of B2 receptors and systemic treatment with lipopolysaccharide induce a glucocorticoid-sensitive upregulation of B1 receptors mediating paw edema in the rat and Formalin-induced nociception in mice. These results suggest that induction of upregulation of B1 receptors may play important roles in controlling inflammatory processes and hyperalgesia.Key words: paw edema, rat, mouse, pain, Formalin test, bradykinin, B1 and B2 agonists and antagonists, lipopolysaccharide, dexamethasone.

scholarly journals Anti-inflammatory, antinociceptive, and antipyretic effects of methanol extract of Cariniana rubra stem bark in animal models

2011 ◽  
Vol 83 (2) ◽  
pp. 557-566 ◽  
Author(s):  
Edson N. Santos ◽  
Joaquim C.S. Lima ◽  
Vânia F. Noldin ◽  
Valdir Cechinel-Filho ◽  
Vietla S. N. Rao ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Vascular Permeability ◽  
Stem Bark ◽  
Bark Extract ◽  
Second Phase ◽  
Traditional Use ◽  
Triterpenoid Saponins ◽  
Rat Paw Edema ◽  
Arjunolic Acid ◽  
Anti Inflammatory

Cariniana rubra Miers (Lecythidaceae), popularly known as "jequitibá-vermelho'', is a large Brazilian tree whose bark is used in infusion and decoction for the treatment of inflammatory conditions. This study aims to assess the anti-inflammatory, antinociceptive, and antipyretic effects of Cariniana rubra methanolic stem bark extract (EM Cr) using experimental animals. Anti-inflammatory activity of EM Cr was tested on carrageenan and dextran-induced rat paw edema, carrageenan-induced pleurisy in rats and acetic acid-increase vascular permeability in mice. Antinociceptive and antipyretic activities were evaluated using acetic acid-induced writhing, formalin and hot-plate tests in mice, as well as brewer's yeast-induced pyrexia in rats. The extract inhibitied carrageenan and dextran-induced edema, reduced exudate volume and leukocyte migration on the carrageenan-induced pleurisy and on the vascular permeability increase induced by acetic acid. The EM Cr inhibited nociception on the acetic acid-induced writhing and in the second phase of formalin test, and decreased rectal temperature. It was, however, inactive against thermal nociception.Phytochemical analysis with EM Cr showed the occurrence of saponins, triterpenes, sterols and phenolic compounds. Phytosterols (β-sitosterol, stigmasterol), pentacyclic triterpenes (α- and β-amyrin as a mixture), arjunolic acid, a phytosterol glycoside (sitosterol 3-O-β-D-glucopyranoside), and triterpenoid saponins (28-β-glucopyranosyl-23-O-acetyl arjunolic acid; 3-O-β-glucopyranosyl arjunolic acid and 28-O-[α-L-Rhamnopyranosyl-(1→2)-β-glucopyranosyl]-23- O-acetyl arjunolic acid) were the main identified compounds. It can be presumed that EM Cr caused their effects by inhibiting the liberation and/or action of different inflammatory mediators. These findings support the traditional use of Cariniana rubra preparations to treat inflammation.

scholarly journals Anti-inflammatory effect of the ethanolic extract from Bowdichia virgilioides H.B.K stem bark

2010 ◽  
Vol 82 (3) ◽  
pp. 609-616 ◽  
Author(s):  
Wander M. Barros ◽  
Vietla S.N. Rao ◽  
Regilane M. Silva ◽  
Joaquim C.S. Lima ◽  
Domingos T.O. Martins
Keyword(s):  
Acetic Acid ◽  
Antinociceptive Effect ◽  
Ethanolic Extract ◽  
Stem Bark ◽  
Significant Inhibition ◽  
Second Phase ◽  
Traditional Use ◽  
Inflammatory Conditions ◽  
Folk Remedy ◽  
Anti Inflammatory

Bowdichia virgilioides H.B.K stem bark (Fabaceae), locally known as "sucupira-preta", is a reputed folk-remedy to treat some inflammatory disorders. To validate its traditional claim, the ethanolic extract from B. virgilioides was evaluated in several animal models of inflammation and nociception. The extract at oral doses of 100 to 1000 mg/kg body weight caused a significant inhibition of carrageenan-induced hind paw oedema, suppression of exudate volume and leukocyte immigration in rat pleurisy induced by carrageenan, and reduction of granuloma weights in the model of subcutaneous granulomas promoted by cotton pellets. In addition, the plant extract significantly inhibited the vascular permeability increase induced by intraperitoneal acetic acid. It also showed marked antinociceptive effect in acetic acid-induced writhing test and in the second phase of formalin test in mice. These findings evidence the anti-inflammatory potential of Bowdichia virgilioides bark and supports its traditional use in inflammatory conditions.

scholarly journals Evaluation of analgesic and anti-inflammatory activities of Hydrocotyle umbellata L., Araliaceae (acariçoba) in mice

2013 ◽  
Vol 85 (3) ◽  
pp. 987-997 ◽  
Author(s):  
IZIARA F. FLORENTINO ◽  
MARCUS VINICIUS M. NASCIMENTO ◽  
PABLINNY M. GALDINO ◽  
ADRIANE F. DE BRITO ◽  
FABIO F. DA ROCHA ◽  
...  
Keyword(s):  
Formalin Test ◽  
Pleural Cavity ◽  
Folk Medicine ◽  
Ethanolic Extract ◽  
Nociceptive Response ◽  
Plasma Extravasation ◽  
Hot Plate Test ◽  
Writhing Test ◽  
Skin Ulcers ◽  
Anti Inflammatory

The Hydrocotyle umbellata L. is a specimen of the Araliaceae family popularly known as acariçoba. Its indications in folk medicine include treatment of skin ulcers, and rheumatism. The aim of this study was to evaluate the antinociceptive and anti-inflammatory activities of the ethanolic extract from acariçoba's underground parts (EEA). EEA reduced the nociceptive response of the animals as evaluated in the acetic acid-induced writhing test and in both phases of formalin test. EEA also presented a supraspinal analgesic activity by increasing the pain latency in the hot plate test. Moreover, EEA reduced the leukocytes migration and plasma extravasation to pleural cavity in the carrageenan-induced pleurisy, besides reducing the edema induced by carrageenan until the second hour and also the edema induced by dextran. In conclusion our results showed that EEA of H. umbellata L. presents analgesic and anti-inflammatory activities, and that a blockade of activity or reduction in the release of different mediators, such as histamine and serotonin, could be involved in these pharmacologic effects.

scholarly journals Comparison of Anti-Inflammatory Effect of Ethanolic Extract of Carica Papaya Leaves and Indomethacin in Carrageenan Induced Rat Paw Edema Animal Model

2019 ◽  
Vol 16 (2) ◽  
pp. 49-53
Author(s):  
Afroza Sultana ◽  
Asma Khan ◽  
Rumana Afroz ◽  
Onayza Yasmeen ◽  
Muqbula Tasrin Aktar ◽  
...  
Keyword(s):  
Animal Model ◽  
Normal Saline ◽  
Carica Papaya ◽  
Ethanolic Extract ◽  
Paw Edema ◽  
Rat Paw Edema ◽  
Group I ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Inflammatory Effect

Background: Carica papaya is very useful vegetables for daily living. Objective: The purpose of the present study was to compare the anti-inflammatory effect of ethanolic extract of Carica papaya leaves and indomethacin in carrageenan induced rat paw edema animal model. Methodology: This was an animal study carried out in the Department of Pharmacology at Dhaka Medical College, Dhaka, Bangladesh during the period from July 2014 to June 2015 for a period of one (01) year. The leaves of Carica Papaya collected from Botanical garden, Mirpur, Dhaka, Bangladesh. The animals were divided into four groups. Inflammation was produced by injecting 0.1 ml of 1% carrageenan solution in normal saline in all experimental animals. Group I were served as control that received normal saline. Group II were received ethanolic extract of Carica Papaya. Group III were received ethanolic extract of Carica Papaya leaves. Group IV were received indomethacin. Result: The experiment was carried out on 48 Long Evan Norwegian rats. Rats were divided in 4 groups of six animals each. The mean initial antero-posterior diameter of rat’s paw of group I, II, III and IV were 3.83±0.04, 2.12±0.02, 1.74±0.01 and 1.60±0.03 respectively. The inhibition of edema formation were 44.64%, 54.57% and 58.22% in group II, III and IV respectively. Conclusion: In conclusion the Carica papaya leaves has anti-inflammatory effect Journal of Science Foundation 2018;16(2):49-53

scholarly journals Antioxidant, analgesic and anti-inflammatory activities of Leucas cephalotes (Roxb.ex Roth) Spreng

2010 ◽  
Vol 46 (3) ◽  
pp. 525-529 ◽  
Author(s):  
Bhukya Baburao ◽  
Anreddy Rama Narsimha Reddy ◽  
Gangarapu Kiran ◽  
Yellu Narsimha Reddy ◽  
Gottumukkala Krishna Mohan
Keyword(s):  
Methanolic Extract ◽  
Maximum Activity ◽  
Dependent Manner ◽  
Hot Plate Test ◽  
Rat Paw Edema ◽  
Whole Plant ◽  
Ic50 Value ◽  
Dpph Method ◽  
Anti Inflammatory ◽  
Rat Paw

The whole plant of the methanolic extract from Leucas cephalotes was screened for invitro antioxidant (using the DPPH method), invivo analgesic (using hot plate test in mice) and anti-inflammatory (using rat paw edema test) activities. The methanolic extract of Leucas cephalotes (MELC) scavenged the DPPH radicals in a dose-dependent manner. The IC50 value to scavenge DPPH radicals was found to be 421.3µg/ml. A significant (p<0.0005) analgesic activity was observed at 60 min with 200 mg/kg, and 400 mg/kg exhibited maximum activity. The maximum anti-inflammatory response was produced at 3 hr and 2 hr with doses of 200 and 400 mg/kg, respectively. These results suggest that the methanolic extract from Leucas cephalotes exerts significant analgesic and anti-inflammatory effects, which were comparable with standard drugs.

scholarly journals Synthesis, Anti-Inflammatory, and Analgesic Activities of Derivatives of 4-Hydroxy-2-benzoxazolone

2015 ◽  
Vol 2015 ◽  
pp. 1-5
Author(s):  
Guangjin Zheng ◽  
Tieyu Chen ◽  
Xing Peng ◽  
Shengjing Long
Keyword(s):  
Chinese Herb ◽  
Primary Amines ◽  
One Pot ◽  
Hot Plate Test ◽  
Rat Paw Edema ◽  
Electrophilic Reagents ◽  
Anti Inflammatory ◽  
Analgesic Activities ◽  
Rat Paw ◽  
Derivatives Of

Benzoxazolones are widely distributed in plants and are of increasing interest for a variety of pharmacological properties, such as detoxification, antibacterial, anti-inflammatory, and analgesic activities and tranquilizers (Michaelidou and Hadjipavlou-Litina, 2005; Doğruer et al., 1998). 4-Hydroxy-2-benzoxazolone (HBOA) is one of the major bioactive compounds in traditional Chinese herb drugAcanthus ilicifolius  (Peng and Long, 2006) which has obvious anti-inflammatory and analgesic activities (Huo et al., 2004; Mani Senthil Kumar et al., 2008; Babu et al., 2001). In this research, we used 2-nitroresorcinol as starting material to prepare HBOA with a novel “one-pot-way.” Derivatives of HBOA TC-2∼TC-4 were obtained via electrophilic reagents and reacted with corresponding primary amines to afford Schiff Base derivatives TC-5∼TC-10 for further study. Anti-inflammatory and analgesic activities of those derivatives were determined by using carrageenan-induced rat paw edema test. The analgesic activities of the derivatives were determined by the hot-plate test.

ANTI-INFLAMMATORY EFFECT OF SEEDS OF TAMARINDUS INDICA IN WISTAR RATS

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (02) ◽  
pp. 49-53
Author(s):  
M. G Hivrale ◽  
◽  
A. A. Mali ◽  
D. D. Bandawane
Keyword(s):  
Ethyl Acetate ◽  
Methanolic Extract ◽  
Inflammatory Activity ◽  
Second Phase ◽  
Tamarindus Indica ◽  
Reference Drug ◽  
Rat Paw Edema ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Indian Medicine

Tamarindus indica L. is highly valued in Indian medicine for management of painful inflammation, diabetes and constipation. In the present study, the anti-inflammatory potential was evaluated using carrageenan induced rat paw edema model. The coarse seed powder was extracted with distilled water, ethyl acetate and methanol and submitted to chemical tests which revealed the presence of alkaloids, tannins, flavonoids, glycosides, phenolic compounds and steroids. All the three extracts i.e. aqueous, ethyl acetate and methanolic extract of Tamarindus indica seeds at 500 mg/kg showed significant anti-inflammatory activity in the second phase of inflammation. For comparison purpose, we used indomethacin (10 mg/kg) as a reference drug. The results showed that methanolic extract exhibited more promising anti-inflammatory activity compared to aqueous and ethyl acetate extracts.

scholarly journals Assessment of analgesic and anti-inflammatory potential of ethanolic extract of Gossypium arboreum leaves in experimental animals

2019 ◽  
Author(s):  
Sharad Patange ◽  
Vijay Navghare ◽  
Prashant Zagade ◽  
Akshay Kendre ◽  
Swarp Jadhav ◽  
...  
Keyword(s):  
Cotton Plant ◽  
Analgesic Activity ◽  
Ethanolic Extract ◽  
Tail Flick ◽  
Gossypium Arboreum ◽  
Paw Edema ◽  
Experimental Animals ◽  
Rat Paw Edema ◽  
Anti Inflammatory ◽  
Rat Paw

Background and objectives: Gossypium arboreum commonly known as cotton plant, this variety of cotton plant available throughout India. Cotton plant was used traditionally for the treatment of infection, diarrhea and other inflammatory conditions. The aim of present study is to evaluate analgesic and anti-inflammatory activity of Gossypium arboreum leaves extract on experimental animals. Materials and Methods: The ethanolic extract of Gossypium arboreum leaves (EEGA) was subjected to assess its antioxidant potential using DPPH radical scavenging assay; further anti-inflammatory and analgesic activity was assessed by using carrageenan-induced rat paw edema and tail flick test respectively in experimental animals. Results: It was observed that free radicals were scavenged by the EEGA in a concentration dependent manner. The Ethanolic extract showed maximum 72% scavenging activities at 200 g/ml concentration. The ethanol extract exhibited significant analgesic activity in the tail-flick model (?&lt;0.01) by increasing the reaction time of the mice to 8.9 sec at 180min after treatment in comparison to control (3.4 sec). The EEGA (100, 200 and 400 mg/kg, p.o.) showed dose-dependent, inhibition of carrageenan-induced rat paw edema from 30 min onwards (P&lt;0.01), Conclusions: Present study revealed that the ethanolic extract of Gossypium arboreum displayed prominent analgesic and anti-inflammatory activity in experimental animals owing to its antioxidant property.

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