scholarly journals Neurocysticercosis: detection of IgG, IgA and IgE antibodies in cerebrospinal fluid, serum and saliva samples by ELISA with Taenia solium and Taenia crassiceps antigens

2000 ◽  
Vol 58 (1) ◽  
pp. 18-24 ◽  
Author(s):  
EDNÉIA CASAGRANDA BUENO ◽  
ADELAIDE JOSÉ VAZ ◽  
LUÍS DOS RAMOS MACHADO ◽  
JOSÉ ANTONIO LIVRAMENTO
Keyword(s):  
Cerebrospinal Fluid ◽  
Sensitivity And Specificity ◽  
Taenia Solium ◽  
Control Group ◽  
Ige Antibodies ◽  
Taenia Crassiceps ◽  
Inactive Form

We assayed samples of cerebrospinal fluid (CSF), serum and saliva from patients with neurocysticercoses, control group and individuals with other parasitoses, by ELISA with Taenia crassiceps vesicular fluid antigen (Tcra) and Taenia solium total antigen (Tso) for the detection of antibodies. The sensitivity for IgG-Tcra was 100% for CSF and serum, and 32.0% for saliva; and for IgG-Tso 100% for CSF, 80.0% for serum and 24.% for saliva. Specificity was 100% for CSF and 80.0% for serum with both antigens, and 100% for saliva with Tcra and 87.5% with Tso. The sensitivity and specificity for IgA-Tcra was, respectively, 40.0% and 100% for CSF, 36.0% and 97.1% for serum, and 4.0% and 90.0% for saliva. IgE detection showed 24.0% sensitivity and 97.1% specificity for serum, with no detection in CSF samples. The search for antibodies revealed the presence of IgG > IgA > IgE in CSF, serum and saliva samples, with IgG being present in all phases of the disease, while IgA/IgE were more frequent in the inactive form.

scholarly journals Evaluation of Taenia solium and Taenia crassiceps cysticercal antigens for immunodiagnosis of neurocysticercosis using ELISA on cerebrospinal fluid samples

2007 ◽  
Vol 40 (2) ◽  
pp. 152-155 ◽  
Author(s):  
Lisandra Akemi Suzuki ◽  
Gisele Cristina Arruda ◽  
Elizabeth Maria Aparecida Barasnevicius Quagliato ◽  
Qláudio Lúcio Rossi
Keyword(s):  
Cerebrospinal Fluid ◽  
Sensitivity And Specificity ◽  
Neurological Disorders ◽  
Taenia Solium ◽  
Igg Antibodies ◽  
Taenia Crassiceps ◽  
Specific Antibodies ◽  
Immunological Reactions

The efficacy of whole parasite and vesicular fluid antigen extracts from Taenia solium and Taenia crassiceps cysticerci for immunodiagnosis of neurocysticercosis was evaluated using ELISA on cerebrospinal fluid samples. Anticysticercal IgG antibodies were assayed in cerebrospinal fluid samples from 23 patients with neurocysticercosis and 35 patients with other neurological disorders. The ELISA reaction for the whole Taenia solium cysticercal extract showed 91.3% sensitivity and 94.3% specificity, whereas the sensitivity and specificity of the ELISA for the whole Taenia crassiceps cysticercal extract were 87% and 94.3%, respectively. The ELISA reactions for vesicular fluid from Taenia solium or Taenia crassiceps showed 91.3% sensitivity and 97.1% specificity. Considering the results obtained from the four antigen preparations, vesicular fluid from Taenia solium and Taenia crassiceps cysticerci may be useful as a source of antigens for immunological reactions that are used for detecting specific antibodies in cerebrospinal fluid samples from patients with neurocysticercosis.

scholarly journals Use of Taenia crassiceps Cysticercus Antigen Preparations for Detection of Antibodies in Cerebrospinal Fluid Samples from Patients with Neurocysticercosis (Taenia solium)

2002 ◽  
Vol 9 (1) ◽  
pp. 190-193 ◽  
Author(s):  
Alessandra Xavier Pardini ◽  
Regina Helena Peralta ◽  
Adelaide José Vaz ◽  
Luis dos Ramos Machado ◽  
José Mauro Peralta
Keyword(s):  
Cerebrospinal Fluid ◽  
Affinity Chromatography ◽  
Molecular Mass ◽  
Sensitivity And Specificity ◽  
Concanavalin A ◽  
Taenia Solium ◽  
Enzyme Linked Immunosorbent Assay ◽  
Good Reproducibility ◽  
Taenia Crassiceps ◽  
Glycoprotein Antigen

ABSTRACT Antigen extracts obtained from the vesicular fluid of Taenia crassiceps cysticerci and from fractions purified by affinity chromatography with the lectin concanavalin A and the glycoprotein antigen separated by electrophoresis were used for the detection of Taenia solium anticysticercus antibodies. The sensitivity and specificity obtained for all antigens were 100% in enzyme-linked immunosorbent assay with good reproducibility. Using immunoblotting of the three antigens, low-molecular-mass peptides (18 and 14 kDa) were characterized only in cerebrospinal fluid samples from patients with neurocysticercosis. The results confirm that antigen fractions purified from T. crassiceps cisticerci are important sources of specific peptides and proved to be efficient in detecting anti-T. solium antibodies.

scholarly journals Enzyme linked immunosorbent assay (ELISA) for the detection of IgG, IgM, IgE and IgA against Cysticercus cellulosae in cerebrospinal fluid of patients with neurocysticercosis

2002 ◽  
Vol 60 (2B) ◽  
pp. 400-405 ◽  
Author(s):  
Newton Satoru Odashima ◽  
Osvaldo Massaiti Takayanagui ◽  
José Fernando de Castro Figueiredo
Keyword(s):  
Cerebrospinal Fluid ◽  
Sensitivity And Specificity ◽  
Immunosorbent Assay ◽  
Neurological Diseases ◽  
Active Form ◽  
Clinical Manifestations ◽  
Enzyme Linked Immunosorbent Assay ◽  
Inactive Form ◽  
Cysticercus Cellulosae ◽  
Inflammation Signs

The objective of this study was to analyze different immunoglobulins classes (IgG, IgM, IgE and IgA) against Cysticercus cellulosae in the cerebrospinal fluid (CSF), through enzyme linked immunosorbent assay (ELISA), correlating them to clinical and tomographic profiles in patients with neurocysticercosis (NCC). Eighty-five specimens of CSF were obtained from 43 cases with NCC (26 with the active form and 17 with the inactive form) and from 42 patients with other neurological diseases. The inactive form of NCC presented a profile in CSF similar to the group without NCC. The active form of NCC presented elevation of specific immunoglobulins (IgG, IgM, IgE, and IgA) in decreasing order, with the highest values being detected among the cases with intraventricular cysts, or with inflammation signs in CSF or in those with multiple clinical manifestations. The highest sensitivity and specificity were obtained with ELISA-IgG (88.5% and 93.2%, respectively). This study confirmed the importance of ELISA in the immunologic diagnosis of NCC.

scholarly journals Specific Taenia crassiceps and Taenia solium Antigenic Peptides for Neurocysticercosis Immunodiagnosis Using Serum Samples

2000 ◽  
Vol 38 (1) ◽  
pp. 146-151
Author(s):  
Ednéia Casagranda Bueno ◽  
Adelaide José Vaz ◽  
Luís Dos Ramos Machado ◽  
JoséAntônio Livramento ◽  
Sílvia Regina Mielle
Keyword(s):  
Taenia Solium ◽  
Severe Infection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Antigenic Peptides ◽  
Serum Samples ◽  
Immunoblotting Analysis ◽  
Taenia Crassiceps ◽  
The Central Nervous System ◽  
Paired Serum

ABSTRACT Neurocysticercosis (NC), i.e., the presence of the larval form of Taenia solium in tissues, is the most frequent and severe infection involving the central nervous system. Paired serum and cerebrospinal fluid (CSF) samples from patients with NC, CSF and serum samples from a control group, and serum samples from patients with other parasitoses were studied by enzyme-linked immunosorbent assay (ELISA) and by immunoblotting with Taenia crassiceps vesicular fluid antigen (Tcra) and Taenia solium total saline antigen (Tso) for the detection of immunoglobulin G antibodies. ELISAs carried out with the Tso and Tcra antigens showed 94.1 and 95.6% sensitivities, respectively, for the detection of antibodies in CSF and 70.6% and 91.2% sensitivities, respectively, for the detection of antibodies in serum, with 100% specificity for the detection of antibodies in CSF and 80% specificity for the detection of antibodies in serum for both antigens. On the basis of the reactivities of the peptides in the samples analyzed, the peptides of ≤23, 39, 85 to 77, and 97 kDa were found to be Tso specific by immunoblotting and the peptides of ≤62, 74, 109, 121, and 131 kDa were found to be Tcra specific. Tests with Tcra extract had higher sensitivities and more homogeneous results and permitted us to obtain the parasites easily. We suggest the use of Tcra ELISA for the study of serum and confirmation of the results for sera positive by an immunoblotting analysis in which specific peptides (e.g., peptides of 19 to 13 kDa) are detected.

scholarly journals Total IgE detection in paired cerebrospinal fluid and serum samples from patients with neurocysticercosis

2000 ◽  
Vol 42 (2) ◽  
pp. 67-70 ◽  
Author(s):  
Ednéia Casagranda BUENO ◽  
Adelaide José VAZ ◽  
Luís dos Ramos MACHADO ◽  
José Antônio LIVRAMENTO
Keyword(s):  
Cerebrospinal Fluid ◽  
Taenia Solium ◽  
Parasitic Infection ◽  
Control Group ◽  
Serum Samples ◽  
Total Ige ◽  
Normal Value ◽  
Significant Difference ◽  
Group A ◽  
The Central Nervous System

Neurocysticercosis (NC), the presence of Taenia solium metacestodes in tissues, is the most frequent and severe parasitic infection of the central nervous system. We investigated the presence of total IgE by an automated chemiluminescence assay in 53 paired cerebrospinal fluid (CSF) and serum samples from patients with NC (P) and in 40 CSF samples from individuals with other neurological disorders as the control group (C). Total IgE concentration ranged from 1.2 to 6.6 IU/ml (mean = 1.4 IU/ml, standard deviation-sd = 1.1 IU/ml) in 28.3% of CSF samples from the P group, a value significantly higher than for the C group (£1.0 IU/ml). The serum samples from the P group showed concentrations ranging from 1.0 to 2330.0 IU/ml (mean = 224.1 IU/ml, sd = 452.1 IU/ml), which were higher than the normal value cited by the manufacturer (<100.0 IU/ml) in 32.1% of the samples. A significant difference was observed in CSF samples from the P and C groups (p = 0.005) and in serum samples from the P group compared to the normal value (p = 0.005), with sera showing more frequent abnormal results.

scholarly journals Evaluation of cysticercus-specific IgG (total and subclasses) and IgE antibody responses in cerebrospinal fluid samples from patients with neurocysticercosis showing intrathecal production of specific IgG antibodies

2013 ◽  
Vol 71 (2) ◽  
pp. 106-109 ◽  
Author(s):  
Lisandra Akemi Suzuki ◽  
Cláudio Lúcio Rossi
Keyword(s):  
Cerebrospinal Fluid ◽  
Neurological Disorders ◽  
Taenia Solium ◽  
Antibody Responses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Ige Antibodies ◽  
Future Studies ◽  
Specific Igg ◽  
Specific Igg Antibodies

In the present study, an enzyme-linked immunosorbent assay (ELISA) standardized with vesicular fluid of Taenia solium cysticerci was used to screen for IgG (total and subclasses) and IgE antibodies in cerebrospinal fluid (CSF) samples from patients with neurocysticercosis showing intrathecal production of specific IgG antibodies and patients with other neurological disorders. The following results were obtained: IgG-ELISA: 100% sensitivity (median of the ELISA absorbances (MEA)=1.17) and 100% specificity; IgG1-ELISA: 72.7% sensitivity (MEA=0.49) and 100% specificity; IgG2-ELISA: 81.8% sensitivity (MEA=0.46) and 100% specificity; IgG3-ELISA: 63.6% sensitivity (MEA=0.12) and 100% specificity; IgG4-ELISA: 90.9% sensitivity (MEA=0.85) and 100% specificity; IgE-ELISA 93.8% sensitivity (MEA=0.60) and 100% specificity. There were no significant differences between the sensitivities and specificities in the detection of IgG-ELISA and IgE-ELISA, although in CSF samples from patients with neurocysticercosis the MEA of the IgG-ELISA was significantly higher than that of the IgE-ELISA. The sensitivity and MEA values of the IgG4-ELISA were higher than the corresponding values for the other IgG subclasses. Future studies should address the contribution of IgG4 and IgE antibodies to the physiopathology of neurocysticercosis.

scholarly journals Detection of IgG in cerebrospinal fluid for diagnosis of neurocysticercosis: evaluation of saline and SDS extracts from Taenia solium and Taenia crassiceps metacestodes by ELISA and immunoblot assay

2001 ◽  
Vol 6 (3) ◽  
pp. 219-226 ◽  
Author(s):  
Ivanildes Solange da Costa Barcelos ◽  
Jose Roberto Mineo ◽  
Deise Aparecida de Oliveira Silva ◽  
Marcelo Simao Ferreira ◽  
Leandro Pajuaba de Moura ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Taenia Solium ◽  
Immunoblot Assay ◽  
Taenia Crassiceps

Immunoblot with Cerebrospinal Fluid from Patients with Neurocysticercosis using Antigen from Cysticerci of Taenia Solium and Taenia Crassiceps

1997 ◽  
Vol 57 (3) ◽  
pp. 354-357 ◽  
Author(s):  
Adelaide J. Vaz ◽  
Jose A. Livramento ◽  
A. Walter Ferreira ◽  
Celia M. Nunes ◽  
Marcos V. Da Silva ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Taenia Solium ◽  
Taenia Crassiceps

scholarly journals Lack of association between parenchymal neurocysticercosis and HLA Class I and Class II antigens

1999 ◽  
Vol 22 (1) ◽  
pp. 7-11
Author(s):  
Eni Picchioni Bompeixe ◽  
Sonia Maria Correia Machado da Costa ◽  
Walter Oleschko Arruda ◽  
Maria Luiza Petzl-Erler
Keyword(s):  
Cerebrospinal Fluid ◽  
Taenia Solium ◽  
Public Health Problem ◽  
Hla Class I ◽  
Host Susceptibility ◽  
Control Group ◽  
Major Public Health Problem ◽  
Significant Difference ◽  
The Central Nervous System ◽  
Control Association

Neurocysticercosis, caused by encysted larvae of the tapeworm Taenia solium, is the most common infection of the central nervous system and a major public health problem in many countries. Prevalence in the region of Curitiba, located in the southern Brazilian State of Paraná, is one of the highest in the world. The genetics of host susceptibility to neurocysticercosis (NCC) is still obscure. To investigate if major histocompatibility complex (MHC) genes influence individual susceptibility to NCC, we performed a case-control association analysis. Fifty-two Caucasoid patients and 149 matched controls were typed for antigens of the HLA-A, B, C, DR and DQ loci. All patients had computerized tomography and clinical features compatible with parenchymal NCC. Indirect immunofluorescence of cerebrospinal fluid showed that 19 (37%) of the patients presented anti-cysticercus antibodies at titers <FONT FACE="Symbol">³</font> 1:10. Frequencies of HLA specificities in the whole group of patients and in the subgroup with antibodies in cerebrospinal fluid were compared to those of the control group. No significant difference was found. These results do not support the hypothesis of HLA gene participation in susceptibility to parenchymal neurocysticercosis.

Assessment and Diagnostic Accuracy Evaluation of the Reflux Symptom Index (RSI) Scale: Psychometric Properties using Optimal Scaling Techniques

2020 ◽  
Vol 129 (10) ◽  
pp. 1020-1029
Author(s):  
Andrea Nacci ◽  
Luca Bastiani ◽  
Maria Rosaria Barillari ◽  
Jerome R. Lechien ◽  
Massimo Martinelli ◽  
...  
Keyword(s):  
Psychometric Properties ◽  
Sensitivity And Specificity ◽  
Reflux Symptom ◽  
Original Version ◽  
Control Group ◽  
Clinical Group ◽  
Accuracy Evaluation ◽  
Reflux Symptom Index ◽  
Characteristic Analysis ◽  
Symptom Index

Objectives: To investigate the psychometric properties of the reflux symptom index (RSI) as short screening approach for the diagnostic of laryngopharyngeal reflux (LPR) in patients with confirmed diagnosed regarding the 24-hour multichannel intraluminal impedance-pH monitoring (MII-pH). Methods: From January 2017 to December 2018, 56 patients with LPR symptoms and 71 healthy individuals (control group) were prospectively enrolled. The LPR diagnosis was confirmed through MII-pH results. All subjects (n = 127) fulfilled RSI and the Reflux Finding Score (RFS) was performed through flexible fiberoptic endoscopy. The sensitivity and the specificity of RSI was assessed by ROC (Receiver Operating Characteristic) analysis. Results: A total of 15 LPR patients (26.8%) of the clinical group met MII-pH diagnostic criteria. Among subjects classified as positive for MII- pH diagnoses, RSI and RFS mean scores were respectively 20 (SD ± 10.5) and 7.1 (SD ± 2.5), values not significantly different compared to the negative MII-pH group. The metric analysis of the items led to the realization of a binary recoding of the score. Both versions had similar psychometric properties, α was 0.840 for RSI original version and 0.836 for RSI binary version. High and comparable area under curve (AUC) values indicate a good ability of both scales to discriminate between individuals with and without LPR pathology diagnosis. Based on balanced sensitivity and specificity, the optimal cut-off scores for LPR pathology were ≥ 5 for RSI binary version and ≥ 15 for RSI original version. Both version overestimated LPR prevalence. The original version had more sensitivity and the RSI Binary version had more specificity. Conclusions: It would be necessary to think about modifying the original RSI in order to improve its sensitivity and specificity (RSI binary version, adding or changing some items), or to introduce new scores in order to better frame the probably affected of LPR patient.

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