scholarly journals Parasitological and immunological diagnoses of strongyloidiasis in immunocompromised and non-immunocompromised children at Uberlândia, state of Minas Gerais, Brazil

2000 ◽  
Vol 42 (1) ◽  
pp. 51-55 ◽  
Author(s):  
Fabiana Martins de PAULA ◽  
Elísio de CASTRO ◽  
Maria do Rosário de Fátima GONÇALVES-PIRES ◽  
Maria das Graças MARÇAL ◽  
Maria B. CAMPOS ◽  
...  
Keyword(s):  
Antibody Test ◽  
Strongyloides Stercoralis ◽  
Elisa Test ◽  
Minas Gerais ◽  
University Hospital ◽  
Serum Samples ◽  
Immunological Tests ◽  
Parasitological Diagnosis ◽  
Immunological Diagnosis ◽  
The University

Parasitological and immunological diagnoses were part of a study conducted among 151 children, 83 immunocompromised (IC) and 68 non-immunocompromised (non-IC) aged from zero to 12, seen at the University Hospital, Universidade Federal de Uberlândia, State of Minas Gerais, Brazil, from February, 1996, to June, 1998. Three fecal samples from each child were analyzed for the parasitological diagnosis by Baermann-Moraes and Lutz methods. The immunological diagnosis to detect IgG and IgM antibodies was carried out by the indirect immunofluorescence antibody test (IFAT) with cryo-microtome sections of Strongyloides stercoralis and Strongyloides ratti larvae as antigens and by the ELISA test with an alkaline extract of S. ratti as the antigens. Of the 151 children 5 (3.31%) were infected with larvae of S. stercoralis (2 cases IC, 2.41%, and 3 cases non-IC, 4.41%). The IFAT-IgG detected 7 (8.43%) serum samples positive among IC, and 2 (2.94%) cases among non-IC. The ELISA-IgG test detected 10 (12.05%) serum samples positive among IC, and 1 (1.47%) case among non-IC. The IFAT-IgM detected 6 (7.22%) positive cases among IC, and 3 (4.41%) cases among non-IC. ELISA-IgM test detected 10 (12.05%) positive cases among IC, and 3 (4.41%) cases among non-IC. It was concluded that the immunological tests can help in the diagnosis of strongyloidiasis in immunocompromised children.

scholarly journals Vitamin D Metabolites and Clinical Outcome in Hospitalized COVID-19 Patients

Nutrients ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 2129
Author(s):  
Sieglinde Zelzer ◽  
Florian Prüller ◽  
Pero Curcic ◽  
Zdenka Sloup ◽  
Magdalena Holter ◽  
...  
Keyword(s):  
Vitamin D ◽  
Degradation Products ◽  
University Hospital ◽  
Vitamin D Metabolites ◽  
Serum Concentrations ◽  
Serum Samples ◽  
Liquid Chromatography Tandem Mass ◽  
Relevant Role ◽  
Metabolite Ratio ◽  
The University

(1) Background: Vitamin D, a well-established regulator of calcium and phosphate metabolism, also has immune-modulatory functions. An uncontrolled immune response and cytokine storm are tightly linked to fatal courses of COVID-19. The present retrospective study aimed to inves-tigate vitamin D status markers and vitamin D degradation products in a mixed cohort of 148 hospitalized COVID-19 patients with various clinical courses of COVID-19. (2) Methods: The serum concentrations of 25(OH)D3, 25(OH)D2, 24,25(OH)2D3, and 25,26(OH)2D3 were determined by a validated liquid-chromatography tandem mass-spectrometry method in leftover serum samples from 148 COVID-19 patients that were admitted to the University Hospital of the Medical Uni-versity of Graz between April and November 2020. Anthropometric and clinical data, as well as outcomes were obtained from the laboratory and hospital information systems. (3) Results: From the 148 patients, 34 (23%) died within 30 days after admission. The frequency of fatal outcomes did not differ between males and females. Non-survivors were significantly older than survivors, had higher peak concentrations of IL-6 and CRP, and required mechanical ventilation more frequently. The serum concentrations of all vitamin D metabolites and the vitamin D metabolite ratio (VMR) did not differ significantly between survivors and non-survivors. Additionally, the need for res-piratory support was unrelated to the serum concentrations of 25(OH)D vitamin D and the two vitamin D catabolites, as well as the VMR. (4) Conclusion: The present results do not support a relevant role of vitamin D for the course and outcome of COVID-19.

scholarly journals Seroepidemiology ofLeishmaniaspp. in equids from Uberlândia, Minas Gerais, Brazil

2017 ◽  
Vol 47 (5) ◽  
Author(s):  
Patrícia Magalhães de Oliveira ◽  
Felipe Garcia ◽  
Fernanda Evers ◽  
Vinícius de Morais Barbosa ◽  
Diego Camilo Mora Obando ◽  
...  
Keyword(s):  
Economic Activity ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Epidemiological Survey ◽  
Minas Gerais ◽  
Southeastern Brazil ◽  
Wild Animals ◽  
Serum Samples ◽  
Leishmania Spp

ABSTRACT: This study evaluated the occurrence of Leishmania spp. in equids from Uberlândia, Minas Gerais, Southeastern Brazil. Serum samples from 257 healthy equids of Uberlândia were assessed for the presence of antibodies against Leishmania spp. by using the indirect fluorescent-antibody test. Additionally, an epidemiological survey was done to evaluate the possible risk factors (species of equids, age, gender, economic activity, and contact with domestic and wild animals) associated with the presence of antibodies against Leishmania spp. The total seroprevalence against Leishmania spp. was 24.1% (62/257); representing seroprevalence levels of 22.7% (55/242) in horses, 50% (4/8) in mules, and 42.9% (3/7) in ponies from all regions evaluated. The species of equid was the only factor that contributed to an elevated seroprevalence of Leishmania spp.

Immune complex detection in saliva samples: an innovative proposal for the diagnosis of human strongyloidiasis

Parasitology ◽  
2017 ◽  
Vol 145 (8) ◽  
pp. 1090-1094 ◽  
Author(s):  
L. R. Bosqui ◽  
A. L. R. Gonçalves ◽  
M. R. F. Gonçalves-Pires ◽  
W. R. Pavanelli ◽  
I. Conchon-Costa ◽  
...  
Keyword(s):  
Immune Complex ◽  
Area Under The Curve ◽  
Strongyloides Stercoralis ◽  
Kappa Index ◽  
Serum Samples ◽  
Worldwide Distribution ◽  
General Index ◽  
Group I ◽  
Parasitological Diagnosis ◽  
Group Ii

AbstractHuman strongyloidiasis is caused by helminth Strongyloides stercoralis. It has a worldwide distribution, often neglected and cause of severe morbidity. The parasitological diagnosis is hindered by the low and irregular amount of larvae in feces. The goal of the present study was to detect IgG and IgG immune complex using conventional serum samples and saliva as alternative samples. We collected samples from 60 individuals, namely: group I composed of 30 healthy individuals; and group II composed of 30 individuals eliminating S. stercoralis larvae in feces. We calculated the area under the curve, general index of diagnostic accuracy, Kappa index and determined the correlations between different diagnostic tests. The detection of IgG levels was performed by an immunoenzymatic assay with alkaline extract of S. venezuelensis larvae as antigen. Positivity of anti-S. stercoralis IgG in serum samples from group I was 3·3%, and from group II 93·3%. The detection of immune complex indicated that group I exhibited 3·3% and group II 56·7%. In the saliva samples, IgG detection was 26·7% for group I and 43·3% for group II. Immune complex was detected in 20% of group I, and 30% of group II. IgG immune complex in conventional serum samples and saliva as alternative samples can be considered biomarkers for the diagnosis of active strongyloidiasis.

scholarly journals Prevalence of intestinal nematodes in alcoholic patients

2002 ◽  
Vol 35 (6) ◽  
pp. 571-574 ◽  
Author(s):  
Maria P. Zago-Gomes ◽  
Kiyoshi F. Aikawa ◽  
Sandro F. Perazzio ◽  
Carlos S. Gonçalves ◽  
Fausto E.L. Pereira
Keyword(s):  
Immune Modulation ◽  
Control Sample ◽  
Strongyloides Stercoralis ◽  
The Other ◽  
University Hospital ◽  
Ethanol Ingestion ◽  
Stool Examination ◽  
Positive Stool ◽  
Three Samples ◽  
The University

We report the results of a retrospective study on the frequency of intestinal nematodes among 198 alcoholic and 440 nonalcoholic patients at the University Hospital Cassiano Antonio Moraes in Vitória, ES, Brazil. The control sample included 194 nonalcoholic patients matched according to age, sex and neighborhood and a random sample of 296 adults admitted at the same hospital. Stool examination by sedimentation method (three samples) was performed in all patients. There was a significantly higher frequency of intestinal nematodes in alcoholics than in controls (35.3% and 19.2%, respectively), due to a higher frequency of Strongyloides stercoralis (21.7% and 4.1%, respectively). Disregarding this parasite, the frequency of the other nematodes was similar in both groups. The higher frequency of S. stercoralis infection in alcoholics could be explained by immune modulation and/or by some alteration in corticosteroid metabolism induced by chronic ethanol ingestion. Corticosteroid metabolites would mimic the worm ecdisteroids, that would in turn increase the fecundity of females in duodenum and survival of larvae. Consequently, the higher frequency of Strongyloides larvae in stool of alcoholics does not necessarily reflect an increased frequency of infection rate, but only an increased chance to present a positive stool examination using sedimentation methods.

scholarly journals Neutralizing antibodies in Brazilian sera against three strains of human immunodeficiency virus type 1 (HIV-1)

1997 ◽  
Vol 39 (6) ◽  
pp. 319-322 ◽  
Author(s):  
Alcyone Artioli MACHADO ◽  
Ivan HIRSCH ◽  
José Fernando de Castro Figueiredo ◽  
Roberto Martinez ◽  
Jean Claude CHERMANN
Keyword(s):  
Neutralizing Antibodies ◽  
University Hospital ◽  
Serum Samples ◽  
Universal Vaccine ◽  
Immunodeficiency Virus ◽  
Different Strains ◽  
The University ◽  
Adult Volunteers ◽  
Hiv 1

The MN strain of HIV-1 is known to be more prevalent in Brazil, the BRU strain is more prevalent in Europe, and the NDK strain in Africa. It has been suggested in the literature to include different strains in the same vaccine against HIV-1. To contribute to the studies for the development of a universal vaccine, the occurrence of antibodies (Ab) against three HIV-1 strains (MN, BRU and NDK) was determined in serum samples from 85 HIV-1-positive patients, adult volunteers seen at the University Hospital of the Faculty of Medicine of Ribeirão Preto-USP. One-hundred tissue culture infective unit (TCIU) of the viruses reacted with serial dilutions of the sera (2x) and with MT4 cells added at a final concentration of 0.3 × 106 cells/ml, and a cytopathic effect was observed on the 7th and 11th days of incubation. Titres of less than 1/50 were considered to be negative. In 129 tests, the sera were negative for one of the three strains: 40 for MN, 29 for BRU and 60 for NDK. There was a predominance of strains MN and BRU, most of them presenting titres from 1/50 to 1/200. Titres for NDK were detected in 25 sera. We conclude that there seems to be a predominance of strains MN and BRU among the individuals from the region tested; however, the detection of sera with positive NKD titres indicates the need for further studies of this strain in other populations and regions of Brazil

scholarly journals Detection of antibodies against Sarcocystis neurona, Neospora caninum and Toxoplasma gondii in horses, dogs and cat

2019 ◽  
Vol 56 (2) ◽  
pp. e152918
Author(s):  
Marilia De Oliveira Koch ◽  
Luciane Maria Laskoski ◽  
Daniel Moura de Aguiar ◽  
Bianca Ressetti da Silva ◽  
Reinaldo Ramos Régio ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Neospora Caninum ◽  
Fluorescent Antibody ◽  
Clinical Pathology ◽  
Antibody Test ◽  
Serum Samples ◽  
Pathology Laboratory ◽  
Sarcocystis Neurona ◽  
Neurological Signs ◽  
The University

The occurrence and distribution of antibodies against Sarcocystis neurona, Neospora caninum and Toxoplasma gondii was investigated in horses, dogs and cats from Curitiba, Paraná state, Brazil. Serum samples were selected from 100 horses, 100 dogs and 100 cats from the routine of the Veterinary Clinical Pathology Laboratory in the Veterinary Hospital of the University of Paraná (UFPR). The 100 dog samples were divided into two groups: 35 samples from dogs with neurological sign (convulsion) and 65 samples from dogs without neurological signs. The animals were adults of different breeds, males and females. Samples were analyzed by indirect fluorescent antibody test (IFAT) for protozoa S. neurona, N. caninum andT. gondii at the following cut-off dilutions: horses: 1:50, 1:50 and 1:16; dogs: 1:50, 1:50 and 1:16; cats: 1:50, 1:50 and 1:50, respectively. The obtained results were 42% of horses, 7% of dogs and 5% of cats seropositive for S. neurona; 58% of horses, 68% of dogs and 42% of cats seropositive to N. caninum, and 36% of horses, 20% of dogs and 21% of cats seropositive for T. gondii. Among the dogs with neurological signs, 8.6%, 68.6% and 25.7% were seropositive for S. neurona, N. caninum and T. gondii, respectively. Among the dogs without neurological signs, 6.2% 67.7% and 16.9% were seropositive for S. neurona, N. caninum and T. gondii, respectively. No statistical difference was found between groups of seropositive dogs for the three protozoa with neurological signs and without neurological signs. Co-infection and high antibody titers were detected. The antibodies against Sarcocystis neurona, Neospora caninum and Toxoplasma gondii were found widely distributed among horses, dogs and cats in the region of Curitiba, state of Paraná, Brazil.   

scholarly journals Comparison of a Clinic-Based ELISA Test Kit with the Immunofluorescence Antibody Test for AssayingLeishmania infantumAntibodies in Dogs

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Daniela Proverbio ◽  
Eva Spada ◽  
Luciana Baggiani ◽  
Giada Bagnagatti De Giorgi ◽  
Roberta Perego
Keyword(s):  
Roc Curve ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Elisa Test ◽  
Standard Test ◽  
Confirmatory Test ◽  
Canine Leishmaniosis ◽  
Igg Antibodies ◽  
Serum Samples ◽  
Test Kit

This study compares a rapid Immunospecific Kalazar Canine Rapid Spot IF with the gold standard test (indirect fluorescent antibody test (IFAT)) for detection ofLeishmania infantumspecific IgG serum antibodies in naturally exposed dogs. Serum samples were obtained from 89 healthy dogs and dogs affected by canine leishmaniosis (CanL). IgG-IFAT titers ≥80 were considered positive. Anti-L. infantumIgG antibodies were found in 54 samples with titers ranging from 1 : 80 to 1 : 5120. The performance of the rapid Immunospecific Kalazar was evaluated using a ROC curve. The area under the ROC curve of 0.957 was significantly different from 0.5 (), and therefore it can be concluded that the rapid Immunospecific Kalazar has the ability to distinguish canine sera with and withoutL. infantumIgG. The best performance of the test was at a cutoff >0 (sensitivity 92.6%, specificity 97%). The test can be used for disease screening if the cutoff is >0 (highest sensitivity, 92.6%) and is recommended as confirmatory test for the presence ofL. infantumIgG antibodies if the cutoff is set >2 (highest specificity, 100%).

scholarly journals ENTERIC PARASITES AND COMMENSALS IN PREGNANT WOMEN SEEN AT THE UNIVERSITY HOSPITAL, FEDERAL UNIVERSITY OF UBERLÂNDIA, STATE OF MINAS GERAIS, BRAZIL

1998 ◽  
Vol 40 (3) ◽  
pp. 193-196 ◽  
Author(s):  
Maria Célia dos SANTOS ◽  
Julia Maria COSTA-CRUZ ◽  
Calil CARVALHO-NETO ◽  
Marcus M. R. LIMA ◽  
Marisa CRISTINA
Keyword(s):  
Pregnant Women ◽  
Minas Gerais ◽  
University Hospital ◽  
Enteric Parasites ◽  
The University

scholarly journals Clinical profile of patients with myasthenia gravis followed at the University Hospital, Federal University of Minas Gerais

2015 ◽  
Vol 61 (2) ◽  
pp. 156-160
Author(s):  
Aline Mansueto Mourão ◽  
Luiz Sérgio Mageste Barbosa ◽  
Elizabeth Regina Comini-Frota ◽  
Denise da Silva Freitas ◽  
Rodrigo Santiago Gomez ◽  
...  
Keyword(s):  
Myasthenia Gravis ◽  
Disease Duration ◽  
Medication Use ◽  
Neuromuscular Diseases ◽  
Case Series ◽  
Minas Gerais ◽  
Clinical Profile ◽  
University Hospital ◽  
The Mean ◽  
The University

Summary Objective: to determine the clinical profile of patients with myasthenia gravis (MG); followed at the Neuromuscular Diseases Clinic of the University Hospital, Federal University of Minas Gerais, Brazil, and to compare it with other Brazilian case series. Methods: sociodemographic and clinical data were collected from patients, and a systematic literature review performed, focusing on national studies on the clinical profile of MG patients. Results: sixty nine patients were enrolled in the study. Fifty five (91%) subjects were female and the mean age (SD) was 37.6 (±11.4) years. The mean disease duration was 14.1 years. Regarding treatment, prednisone was the most used strategy (64%), followed by the use of azathioprine (43%). There was no difference between thymectomized (42) and non-thymectomized (27) patients regarding disease severity and medication use. Conclusion: clinical and socio-demographic features of this MG sample from a University-based clinic resemble those reported in other Brazilian series and in the international literature.

scholarly journals Six-month antibody response to SARS-CoV-2 in healthcare workers assessed by virus neutralisation and commercial assays

2020 ◽  
Author(s):  
Antonin Bal ◽  
Mary-Anne Trabaud ◽  
Jean-Baptiste Fassier ◽  
Muriel Rabilloud ◽  
Kahina Saker ◽  
...  
Keyword(s):  
Antibody Response ◽  
Healthcare Workers ◽  
Disease Onset ◽  
University Hospital ◽  
Serum Samples ◽  
Live Virus ◽  
N Protein ◽  
A Prospective Study ◽  
The University

AbstractWe conducted a prospective study in healthcare workers (n=296) of the University Hospital of Lyon, France. Serum samples (n=296) collected six months after disease onset were tested using three commercial assays: the Wantai Ab assay detecting total antibodies against the receptor binding domain (RBD) of the S protein, the bioMerieux Vidas assay detecting IgG to the RBD and the Abbott Architect assay detecting IgG to the N protein. The neutralising antibody (NAb) titre was also determined for all samples with a virus neutralisation assay (VNA) using live virus. The positivity rate was 100% with the Wantai assay, 84.8% with the bioMerieux assay and 55.4% with the Abbott assay. Only 51% of HCWs were positive for the presence of NAb. Less than 10 % of HCWs had a NAb titre greater than 80. At a neutralising titre of 80, the area under the curves [IC 95%] was 0.71 [0.62-0.81], 0.75 [0.65-0.85] and 0.95 [0.92-0.97] for Wantai, Abbott and Vidas respectively. The data presented herein suggest that commercial assays detecting antibodies against the N protein must not be used in long-term seroprevalence surveys while the Wantai assay could be useful for this purpose. VNA should remain the gold standard to assess the protective antibody response, but some commercial assays could be used as first-line screening of long-term presence of NAb.

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