scholarly journals Assessment of the rapid test based on an immunochromatography technique for detecting anti-Treponema pallidum antibodies

2003 ◽  
Vol 45 (6) ◽  
pp. 319-322 ◽  
Author(s):  
Neuza Satomi Sato ◽  
Carmen Silvia de Melo ◽  
Lia C.M.S. Zerbini ◽  
Edilene P.R. Silveira ◽  
Luiz Jorge Fagundes ◽  
...  
Keyword(s):  
Predictive Value ◽  
Sexually Transmitted Disease ◽  
Transmitted Disease ◽  
Treponema Pallidum ◽  
Rapid Test ◽  
Serum Samples ◽  
Diagnostic Features ◽  
Sexually Transmitted ◽  
Syphilis Screening ◽  
Latent Syphilis

A rapid test based on an immunochromatography assay - Determine™ Syphilis TP (Abbott Lab.) for detecting specific antibodies to Treponema pallidum was evaluated against serum samples from patients with clinical, epidemiological and serological diagnosis of syphilis, patients with sexually transmitted disease other than syphilis, and individuals with negative serology for syphilis. The Determine™ test presented the sensitivity of 93.6%, specificity of 92.5%, and positive predictive value and negative predictive value of 95.2% and 93.7%, respectively. One serum sample from patient with recent latent syphilis showed a prozone reaction. Determine™ is a rapid assay, highly specific and easy to perform. This technique obviates the need of equipment and its diagnostic features demonstrate that it may be applicable as an alternative assay for syphilis screening under some emergency conditions or for patients living in remote localities.

scholarly journals Treponema pallidumNucleic Acid Amplification Testing To Augment Syphilis Screening among Men Who Have Sex with Men

2019 ◽  
Vol 57 (8) ◽  
Author(s):  
Matthew Golden ◽  
Meghan O’Donnell ◽  
Sheila Lukehart ◽  
Paul Swenson ◽  
Paul Hovey ◽  
...  
Keyword(s):  
Transmitted Disease ◽  
Treponema Pallidum ◽  
Secondary Syphilis ◽  
23S Rrna ◽  
Serological Testing ◽  
Content Type ◽  
Sexually Transmitted ◽  
Syphilis Screening ◽  
Sex With Men ◽  
Latent Syphilis

ABSTRACTSyphilis rates in much of the world are now at their highest levels in almost three decades, and new approaches to controlling syphilis, including diagnostic tests with shorter window periods, are urgently needed. We compared the sensitivity of syphilis serological testing using the rapid plasma reagin (RPR) test with that of the combination of serological testing and an experimental 23S rRNATreponema pallidumreal-time transcription-mediated amplification (TMA) assay performed on rectal and pharyngeal mucosal swabs.T. pallidumPCR assays for thetpp47gene were performed on all TMA-positive specimens, as well as specimens from 20 randomly selected TMA-negative men. A total of 545 men who have sex with men (MSM) who were seen in a sexually transmitted disease clinic provided 506 pharyngeal specimens and 410 rectal specimens with valid TMA results. Twenty-two men (4%) were diagnosed with syphilis on the basis of positive RPR test results and clinical diagnoses, including 3 men with primary infections, 8 with secondary syphilis, 9 with early latent syphilis, 1 with late latent syphilis, and 1 with an unstaged infection. Two additional men were diagnosed based on positive rectal mucosal TMA assay results alone, and both also tested positive by PCR assay. At least 1 specimen was TMA positive for 12 of 24 men with syphilis (sensitivity, 50% [95% confidence interval [CI], 29 to 71%]). RPR testing and clinical diagnosis were 92% sensitive (95% CI, 73 to 99%) in identifying infected men. Combining mucosal TMA testing and serological testing may increase the sensitivity of syphilis screening in high-risk populations.

scholarly journals Characterization and Serologic Analysis of the Treponema pallidum Proteome

2010 ◽  
Vol 78 (6) ◽  
pp. 2631-2643 ◽  
Author(s):  
Melanie A. McGill ◽  
Diane G. Edmondson ◽  
James A. Carroll ◽  
Richard G. Cook ◽  
Ralph S. Orkiszewski ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Transmitted Disease ◽  
Treponema Pallidum ◽  
Immunoblot Analysis ◽  
Serum Samples ◽  
Syphilis Infection ◽  
Two Dimensional Gel Electrophoresis ◽  
Ionization Time ◽  
Sexually Transmitted ◽  
Human Sera

ABSTRACT Treponema pallidum subsp. pallidum is the causative agent of syphilis, a sexually transmitted disease characterized by widespread tissue dissemination and chronic infection. In this study, we analyzed the proteome of T. pallidum by the isoelectric focusing (IEF) and nonequilibrating pH gel electrophoresis (NEPHGE) forms of two-dimensional gel electrophoresis (2DGE), coupled with matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) analysis. We determined the identity of 148 T. pallidum protein spots, representing 88 T. pallidum polypeptides; 63 of these polypeptides had not been identified previously at the protein level. To examine which of these proteins are important in the antibody response to syphilis, we performed immunoblot analysis using infected rabbit sera or human sera from patients at different stages of syphilis infection. Twenty-nine previously described antigens (predominantly lipoproteins) were detected, as were a number of previously unidentified antigens. The reactivity patterns obtained with sera from infected rabbits and humans were similar; these patterns included a subset of antigens reactive with all serum samples tested, including CfpA, MglB-2, TmpA, TmpB, flagellins, and the 47-kDa, 17-kDa, and 15-kDa lipoproteins. A unique group of antigens specifically reactive with infected human serum was also identified and included the previously described antigen TpF1 and the hypothetical proteins TP0584, TP0608, and TP0965. This combined proteomic and serologic analysis further delineates the antigens potentially useful as vaccine candidates or diagnostic markers and may provide insight into the host-pathogen interactions that occur during T. pallidum infection.

scholarly journals Imunopatogenesis Treponema pallidum dan Pemeriksaan Serologi

2014 ◽  
Vol 3 (3) ◽  
Author(s):  
Efrida Efrida ◽  
Elvinawaty Elvinawaty
Keyword(s):  
Clinical Symptoms ◽  
Early Stage ◽  
Transmitted Disease ◽  
Treponema Pallidum ◽  
Secondary Syphilis ◽  
Major Health Problem ◽  
Sexually Transmitted ◽  
Tertiary Syphilis ◽  
History Of ◽  
Latent Syphilis

AbstrakSifilis adalah penyakit menular seksual yang sangat infeksius, disebabkan oleh bakteri berbentuk spiral, Treponema pallidum subspesies pallidum. Penyebaran sifilis di dunia telah menjadi masalah kesehatan yang besar dengan jumlah kasus 12 juta pertahun. Infeksi sifilis dibagi menjadi sifilis stadium dini dan lanjut. Sifilis stadium dini terbagi menjadi sifilis primer, sekunder, dan laten dini. Sifilis stadium lanjut termasuk sifilis tersier (gumatous, sifilis kardiovaskular dan neurosifilis) serta sifilis laten lanjut. Sifilis primer didiagnosis berdasarkan gejala klinis ditemukannya satu atau lebih chancre (ulser). Sifilis sekunder ditandai dengan ditemukannya lesi mukokutaneus yang terlokalisir atau difus dengan limfadenopati. Sifilis laten tanpa gejala klinis sifilis dengan pemeriksaan nontreponemal dan treponemal reaktif, riwayat terapi sifilis dengan titer uji nontreponemal yang meningkat dibandingkan dengan hasil titer nontreponemal sebelumnya. Sifilis tersier ditemukan guma dengan pemeriksaan treponemal reaktif, sekitar 30% dengan uji nontreponemal yang tidak reaktifKata kunci: sifilis, Treponema pallidum, serologiAbstractSyphilis is a sexually transmitted disease that is highly infectious, caused by a spiral -shaped bacterium, Treponema pallidum subspecies pallidum. The spread of syphilis in the world has become a major health problem and the common, the number of 12 million cases per year. Infectious syphilis is divided into early and late-stage syphilis. Early-stage syphilis is divided into primary, secondary, and early latent. Advanced stage of syphilis include tertiary syphilis (gumatous, cardiovascular syphilis, and neurosyphilis) and late latent syphilis. Primary syphilis is diagnosed by clinical symptoms of the discovery of one or more chancre (ulcer). Secondary syphilis is characterized by the finding of localized mucocutaneous lesions or with diffuse lymphadenopathy. Latent syphilis without clinical symptoms of syphilis with a nontreponemal and treponemal reactive examination, history of syphilis therapy in nontreponemal test titer increased compared with the results of previous nontreponemal titers. Tertiary syphilis is found guma with reactive treponemal examination, approximately 30% of the non- reactive nontreponemal testKeywords: syphilis, Treponema pallidum, serologi

scholarly journals Laboratory Examination of Syphilis

2021 ◽  
Vol 5 (3) ◽  
pp. 722-741
Author(s):  
M. Izazi Hari Purwoko ◽  
Mutia Devi ◽  
Suroso Adi Nugroho ◽  
Fitriani Fitriani ◽  
Raden Pamudji ◽  
...  
Keyword(s):  
Venereal Disease ◽  
Transmitted Disease ◽  
Treponema Pallidum ◽  
Clinical Manifestations ◽  
Rapid Test ◽  
Dark Field ◽  
Morphological Observation ◽  
Serologic Test ◽  
Sexually Transmitted ◽  
Antibody Absorption

Syphilis, is sexually transmitted disease caused by spirochete Treponema pallidum subsp.pallidum. It have many diverse clinical manifestations that occur in distinct stages. Early diagnosis and management are the main things to prevent transmission and complication. Direct test or morphological observation is the definitive diagnosis of syphilis. This can be done through animal inoculation test, dark field microscopy, direct fluorescence antibody (DFA), and nucleid acid amplification test (NAAT). While the indirect test is a nontreponemal serologic test consist of Wasserman test, venereal disease research laboratory (VDRL), toluidine red unheated serum test (TRUST), unheated serum reagin (USR), rapid plasma reagin (RPR) and treponemal serologic test, such as T. pallidum passive particle agglutination (TPPA), T. pallidum haemagglutination assay (TPHA), fluorescent treponemal antibody absorption (FTA-Abs), enzyme immunoassay (EIA) and rapid test. The algorithm of serologic test can be divided into traditional or reverse.

scholarly journals Syphilis Fast Latex Agglutination Test, a Rapid Confirmatory Test

2001 ◽  
Vol 8 (4) ◽  
pp. 841-842 ◽  
Author(s):  
Martha B. Fears ◽  
Victoria Pope
Keyword(s):  
Point Of Care ◽  
Transmitted Disease ◽  
Treponema Pallidum ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test ◽  
Confirmatory Test ◽  
Serum Samples ◽  
Sexually Transmitted ◽  
Particle Agglutination

ABSTRACT Using 255 serum samples with various reactivities, we evaluated the Syphilis Fast latex agglutination test (Syphilis Fast) against theTreponema pallidum particle agglutination test (TP-PA) for confirming a diagnosis of syphilis. We found 98.8% agreement between the Syphilis Fast and the TP-PA. The Syphilis Fast, however, had a couple of advantages over the TP-PA: the test takes only 8 min to perform and produces results that are easy to read. It appears to be a good confirmatory test for syphilis, especially for point-of-care clinics such as prenatal or sexually transmitted disease clinics.

scholarly journals Laboratory Examination of Syphilis

2021 ◽  
Vol 5 (8) ◽  
pp. 726-745
Author(s):  
M. Izazi Hari Purwoko ◽  
Mutia Devi ◽  
Suroso Adi Nugroho ◽  
Fitriani Fitriani ◽  
Raden Pamudji ◽  
...  
Keyword(s):  
Venereal Disease ◽  
Transmitted Disease ◽  
Treponema Pallidum ◽  
Clinical Manifestations ◽  
Rapid Test ◽  
Dark Field ◽  
Morphological Observation ◽  
Serologic Test ◽  
Sexually Transmitted ◽  
Antibody Absorption

Syphilis, is sexually transmitted disease caused by spirochete Treponema pallidum subsp.pallidum. It have many diverse clinical manifestations that occur in distinct stages. Early diagnosis and management are the main things to prevent transmission and complication. Direct test or morphological observation is the definitive diagnosis of syphilis. This can be done through animal inoculation test, dark field microscopy, direct fluorescence antibody (DFA), and nucleid acid amplification test (NAAT). While the indirect test is a nontreponemal serologic test consist of Wasserman test, venereal disease research laboratory (VDRL), toluidine red unheated serum test (TRUST), unheated serum reagin (USR), rapid plasma reagin (RPR) and treponemal serologic test, such as T. pallidum passive particle agglutination (TPPA), T. pallidum haemagglutination assay (TPHA), fluorescent treponemal antibody absorption (FTA-Abs), enzyme immunoassay (EIA) and rapid test. The algorithm of serologic test can be divided into traditional or reverse.

scholarly journals Clinical and Evolutive Features in Syphilis - HIV Coinfection

2020 ◽  
Vol 25 (4) ◽  
pp. 12-15
Author(s):  
Gabriela Mariana Iancu ◽  
Liliana Elena Todan ◽  
Maria Rotaru
Keyword(s):  
Hiv Infection ◽  
Sexually Transmitted Diseases ◽  
Sexually Transmitted Disease ◽  
Transmitted Disease ◽  
Treponema Pallidum ◽  
Systemic Infection ◽  
The Other ◽  
Local Inflammation ◽  
Sexually Transmitted ◽  
Hiv Coinfection

Abstract Syphilis is a systemic infection caused by Treponema Pallidum spirochete, which is considered to be the main sexually transmitted disease. The genital ulcerations and local inflammation found in syphilis are favouring factors involved in transmitting the HIV infection. Recent data suggests that individuals suffering from other sexually transmitted diseases are 3 to 5 times more exposed to developing HIV infection. On the other hand, simultaneous HIV infection may worsen the syphilis evolution. In the light of this data we present the case of a patient with syphilis-HIV coinfection recently diagnosed and we will summarize the clinical and evolutive features of the syphilis-HIV coinfection.

scholarly journals ANALYSIS OF Treponema pallidum RECOMBINANT ANTIGENS FOR DIAGNOSIS OF SYPHILIS BY WESTERN BLOTTING TECHNIQUE

1999 ◽  
Vol 41 (2) ◽  
pp. 115-118 ◽  
Author(s):  
Neuza Satomi SATO ◽  
Mário H. HIRATA ◽  
Rosário D.C. HIRATA ◽  
Lia Carmen M.S. ZERBINI ◽  
Edilene P.R. SILVEIRA ◽  
...  
Keyword(s):  
Western Blotting ◽  
Blood Donors ◽  
Transmitted Disease ◽  
Treponema Pallidum ◽  
Serum Samples ◽  
Weak Reaction ◽  
Recombinant Antigens ◽  
Sexually Transmitted ◽  
Blotting Technique ◽  
Almost All

Three GST fusion recombinant antigen of Treponema pallidum, described as GST-rTp47, GST-rTp17 and GST-rTp15 were analyzed by Western blotting techniques. We have tested 53 serum samples: 25 from patients at different clinical stages of syphilis, all of them presenting anti-treponemal antibody, 25 from healthy blood donors and three from patients with sexually transmitted disease (STD) other than syphilis. Almost all samples from patients with syphilis presented a strong reactivity with GST-rTp17 antigen. Some samples were non-reactive or showed a weak reaction with GST-rTp47 and/or GST-rTp15, and apparently there was no correlation with the stage of disease. There was no seropositivity among blood donors. No sample reacted with purified GST. We concluded that due to their specificity these recombinant antigens can be used as GST fusion protein for development of syphilis diagnostic assays.

scholarly journals A Case of Secondary Syphilis with the Extragenital Chancre on the Nipple

2020 ◽  
Vol 2020 ◽  
pp. 1-3
Author(s):  
Xiao Yan Wang ◽  
Juliandri ◽  
Zi Jing Liu ◽  
Jia Wen Zhang ◽  
Yun Yi Liu ◽  
...  
Keyword(s):  
Skin Biopsy ◽  
Sexually Transmitted Disease ◽  
Transmitted Disease ◽  
Treponema Pallidum ◽  
Signs And Symptoms ◽  
Secondary Syphilis ◽  
Primary Stage ◽  
Sexually Transmitted ◽  
Serological Examination

Syphilis is a sexually transmitted disease caused by Treponema pallidum. The signs and symptoms of syphilis vary depending on which of the four stages it presents. The primary stage of syphilis classically presents with a painless ulcer (chancre). We report a case of the extragenital chancre on the nipple which is examined from skin biopsy and immunohistochemistry. This case showed that it is important to identify the special site’s pruritus erythema by pathology and serological examination.

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