scholarly journals Treponema pallidumNucleic Acid Amplification Testing To Augment Syphilis Screening among Men Who Have Sex with Men

2019 ◽  
Vol 57 (8) ◽  
Author(s):  
Matthew Golden ◽  
Meghan O’Donnell ◽  
Sheila Lukehart ◽  
Paul Swenson ◽  
Paul Hovey ◽  
...  
Keyword(s):  
Transmitted Disease ◽  
Treponema Pallidum ◽  
Secondary Syphilis ◽  
23S Rrna ◽  
Serological Testing ◽  
Content Type ◽  
Sexually Transmitted ◽  
Syphilis Screening ◽  
Sex With Men ◽  
Latent Syphilis

ABSTRACTSyphilis rates in much of the world are now at their highest levels in almost three decades, and new approaches to controlling syphilis, including diagnostic tests with shorter window periods, are urgently needed. We compared the sensitivity of syphilis serological testing using the rapid plasma reagin (RPR) test with that of the combination of serological testing and an experimental 23S rRNATreponema pallidumreal-time transcription-mediated amplification (TMA) assay performed on rectal and pharyngeal mucosal swabs.T. pallidumPCR assays for thetpp47gene were performed on all TMA-positive specimens, as well as specimens from 20 randomly selected TMA-negative men. A total of 545 men who have sex with men (MSM) who were seen in a sexually transmitted disease clinic provided 506 pharyngeal specimens and 410 rectal specimens with valid TMA results. Twenty-two men (4%) were diagnosed with syphilis on the basis of positive RPR test results and clinical diagnoses, including 3 men with primary infections, 8 with secondary syphilis, 9 with early latent syphilis, 1 with late latent syphilis, and 1 with an unstaged infection. Two additional men were diagnosed based on positive rectal mucosal TMA assay results alone, and both also tested positive by PCR assay. At least 1 specimen was TMA positive for 12 of 24 men with syphilis (sensitivity, 50% [95% confidence interval [CI], 29 to 71%]). RPR testing and clinical diagnosis were 92% sensitive (95% CI, 73 to 99%) in identifying infected men. Combining mucosal TMA testing and serological testing may increase the sensitivity of syphilis screening in high-risk populations.

scholarly journals Imunopatogenesis Treponema pallidum dan Pemeriksaan Serologi

2014 ◽  
Vol 3 (3) ◽  
Author(s):  
Efrida Efrida ◽  
Elvinawaty Elvinawaty
Keyword(s):  
Clinical Symptoms ◽  
Early Stage ◽  
Transmitted Disease ◽  
Treponema Pallidum ◽  
Secondary Syphilis ◽  
Major Health Problem ◽  
Sexually Transmitted ◽  
Tertiary Syphilis ◽  
History Of ◽  
Latent Syphilis

AbstrakSifilis adalah penyakit menular seksual yang sangat infeksius, disebabkan oleh bakteri berbentuk spiral, Treponema pallidum subspesies pallidum. Penyebaran sifilis di dunia telah menjadi masalah kesehatan yang besar dengan jumlah kasus 12 juta pertahun. Infeksi sifilis dibagi menjadi sifilis stadium dini dan lanjut. Sifilis stadium dini terbagi menjadi sifilis primer, sekunder, dan laten dini. Sifilis stadium lanjut termasuk sifilis tersier (gumatous, sifilis kardiovaskular dan neurosifilis) serta sifilis laten lanjut. Sifilis primer didiagnosis berdasarkan gejala klinis ditemukannya satu atau lebih chancre (ulser). Sifilis sekunder ditandai dengan ditemukannya lesi mukokutaneus yang terlokalisir atau difus dengan limfadenopati. Sifilis laten tanpa gejala klinis sifilis dengan pemeriksaan nontreponemal dan treponemal reaktif, riwayat terapi sifilis dengan titer uji nontreponemal yang meningkat dibandingkan dengan hasil titer nontreponemal sebelumnya. Sifilis tersier ditemukan guma dengan pemeriksaan treponemal reaktif, sekitar 30% dengan uji nontreponemal yang tidak reaktifKata kunci: sifilis, Treponema pallidum, serologiAbstractSyphilis is a sexually transmitted disease that is highly infectious, caused by a spiral -shaped bacterium, Treponema pallidum subspecies pallidum. The spread of syphilis in the world has become a major health problem and the common, the number of 12 million cases per year. Infectious syphilis is divided into early and late-stage syphilis. Early-stage syphilis is divided into primary, secondary, and early latent. Advanced stage of syphilis include tertiary syphilis (gumatous, cardiovascular syphilis, and neurosyphilis) and late latent syphilis. Primary syphilis is diagnosed by clinical symptoms of the discovery of one or more chancre (ulcer). Secondary syphilis is characterized by the finding of localized mucocutaneous lesions or with diffuse lymphadenopathy. Latent syphilis without clinical symptoms of syphilis with a nontreponemal and treponemal reactive examination, history of syphilis therapy in nontreponemal test titer increased compared with the results of previous nontreponemal titers. Tertiary syphilis is found guma with reactive treponemal examination, approximately 30% of the non- reactive nontreponemal testKeywords: syphilis, Treponema pallidum, serologi

Confirmatory serological testing of blood donors positive on TPHA screening in Singapore

1997 ◽  
Vol 8 (12) ◽  
pp. 760-763 ◽  
Author(s):  
S S Wong ◽  
D L T Teo ◽  
R K W Chan
Keyword(s):  
Blood Donors ◽  
Congenital Syphilis ◽  
Past History ◽  
Treponema Pallidum ◽  
Secondary Syphilis ◽  
Serological Testing ◽  
Sexually Transmitted ◽  
Blood Transfusion Service ◽  
History Of ◽  
Latent Syphilis

Summary: Seventy-two blood donors who were tested positive by the Singapore Blood Transfusion Service (SBTS) for Treponema pallidum haemagglutination (TPHA) test, were evaluated at the Department of Sexually Transmitted Diseases Clinic (DSC) between November 1994 to December 1996. All underwent syphilis serological testing, including rapid plasma reagin test (RPR), TPHA test and fluorescent treponemal antibody-absorption (FTA-Abs) test. All except one (98.6%) were confirmed TPHA positive by the DSC. Of the 71 TPHA-confirmed-positive donors, 53 (74.6%) were subsequently tested positive for FTA-Abs and 18 (25.4%) were tested negative for FTA-Abs. Twenty-two (31%) of the 71 TPHA-positive blood donors had reactive RPR and 49 (69%) had non-reactive RPR. Of the 22 TPHA-positive donors who had reactive RPR, 19 (86%) had positive FTA-Abs (13 late latent syphilis, 4 serological scar, one late congenital syphilis, one secondary syphilis), and 3 (14%) had negative FTA-Abs (all late latent syphilis). Of the 49 TPHA-positive donors who had non-reactive RPR, 34 (69%) had positive FTA-Abs (24 late latent syphilis, 9 serological scar, one late congenital syphilis) and 15 (31%) had negative FTA-Abs (12 late latent syphilis, 2 serological scar, one false-positive TPHA). Only one TPHA-positive donor referred by the SBTS subsequently turned out to have negative syphilis serology at the DSC. Overall, 68 (95.8%) TPHApositive donors who had a past history of sexual exposure were managed as treated or untreated syphilis, regardless of their RPR or FTA-Abs results. However, FTAAbs was found to be useful in the management of 3 (4.2%) TPHA-positive blood donors in the absence of a history of sexual exposures.

scholarly journals Assessment of the rapid test based on an immunochromatography technique for detecting anti-Treponema pallidum antibodies

2003 ◽  
Vol 45 (6) ◽  
pp. 319-322 ◽  
Author(s):  
Neuza Satomi Sato ◽  
Carmen Silvia de Melo ◽  
Lia C.M.S. Zerbini ◽  
Edilene P.R. Silveira ◽  
Luiz Jorge Fagundes ◽  
...  
Keyword(s):  
Predictive Value ◽  
Sexually Transmitted Disease ◽  
Transmitted Disease ◽  
Treponema Pallidum ◽  
Rapid Test ◽  
Serum Samples ◽  
Diagnostic Features ◽  
Sexually Transmitted ◽  
Syphilis Screening ◽  
Latent Syphilis

A rapid test based on an immunochromatography assay - Determine™ Syphilis TP (Abbott Lab.) for detecting specific antibodies to Treponema pallidum was evaluated against serum samples from patients with clinical, epidemiological and serological diagnosis of syphilis, patients with sexually transmitted disease other than syphilis, and individuals with negative serology for syphilis. The Determine™ test presented the sensitivity of 93.6%, specificity of 92.5%, and positive predictive value and negative predictive value of 95.2% and 93.7%, respectively. One serum sample from patient with recent latent syphilis showed a prozone reaction. Determine™ is a rapid assay, highly specific and easy to perform. This technique obviates the need of equipment and its diagnostic features demonstrate that it may be applicable as an alternative assay for syphilis screening under some emergency conditions or for patients living in remote localities.

scholarly journals Performance of the 47-Kilodalton Membrane Protein versus DNA Polymerase I Genes for Detection of Treponema pallidum by PCR in Ulcers

2014 ◽  
Vol 53 (3) ◽  
pp. 976-980 ◽  
Author(s):  
Angèle Gayet-Ageron ◽  
Frédéric Laurent ◽  
Jacques Schrenzel ◽  
Béatrice Charton ◽  
Gisela Jimenez-Getaz ◽  
...  
Keyword(s):  
Target Gene ◽  
Treponema Pallidum ◽  
Secondary Syphilis ◽  
Dna Polymerase I ◽  
Perfect Agreement ◽  
Content Type ◽  
Sexually Transmitted ◽  
Polymerase I ◽  
Pcr Targeting ◽  
Protein Versus

Treponema pallidumPCR (Tp-PCR) is a direct diagnostic method for primary and secondary syphilis, but there is no recommendation regarding the best choice of target gene. In this study, we sequentially tested 272 specimens from patients with sexually transmitted ulcers usingTp-PCR targeting thetpp47and thenpolAgenes. The two methods showed similar accuracies and an almost-perfect agreement.

scholarly journals Molecular Typing and Macrolide Resistance Analyses ofTreponema pallidumin Heterosexuals and Men Who Have Sex with Men in Japan, 2017

2018 ◽  
Vol 57 (1) ◽  
Author(s):  
Mizue Kanai ◽  
Yuzo Arima ◽  
Shingo Nishiki ◽  
Ken Shimuta ◽  
Ichiro Itoda ◽  
...  
Keyword(s):  
Molecular Typing ◽  
Treponema Pallidum ◽  
Macrolide Resistance ◽  
23S Rrna ◽  
Rrna Gene ◽  
Content Type ◽  
Heterosexual Women ◽  
23S Rrna Gene ◽  
Strain Type ◽  
Sex With Men

ABSTRACTIn recent years, syphilis notifications have increased dramatically in Japan. We carried out molecular typing and macrolide resistance analyses ofTreponema pallidumsubsp.pallidumsamples collected from patients at four clinics and a hospital in Tokyo and Osaka prefectures in 2017. The macrolide resistant strain type 14d/f (SS14-like clade) was found in significantly more cases of syphilis among heterosexuals than in those among men who have sex with men (MSM); i.e., 79% (31/39) of the strains from heterosexuals were 14d/f compared to 37% (7/19) of those from MSM (odds ratio [OR], 6.6; 95% confidence interval [CI], 1.7 to 26.7;P = 0.002). In addition, 83% (50/60) of the strains were identified as macrolide resistant with an A2058G mutation in the 23S rRNA gene; 90% (35/39) of the strains from heterosexuals were macrolide resistant compared to 58% (11/19) of those from MSM. The odds of having the resistant mutation were considerably higher in the former (OR, 6.4; 95% CI, 1.3 to 33.5;P = 0.02). Heterosexual women and heterosexual men showed similar distributions, and the association remained the same when restricted to men. The strain type distribution and the prevalence of macrolide resistance differed substantially between syphilis strains from heterosexual cases and from MSM cases, suggesting distinct epidemiologic profiles for the two communities and providing important insight into the dynamics of syphilis in Japan.

Annular and arciform lesions of the palms as unique manifestations of secondary syphilis

2020 ◽  
Vol 31 (13) ◽  
pp. 1323-1326
Author(s):  
Maria Parodi ◽  
Giulia Ciccarese ◽  
Francesco Drago ◽  
Emanuele Cozzani ◽  
Cinzia Buligan ◽  
...  
Keyword(s):  
Inflammatory Infiltrate ◽  
Treponema Pallidum ◽  
Skin Lesions ◽  
Secondary Syphilis ◽  
Vascular Involvement ◽  
Skin Manifestations ◽  
Sex With Men ◽  
Atypical Presentations ◽  
Latent Syphilis

We describe a patient presenting with annular-arciform symmetric lesions of the palms as the unique manifestation of secondary syphilis. The polymorphism of skin lesions in secondary syphilis depends entirely on the degree of inflammatory infiltrate, the level of vascular involvement and the resulting ischemia of the skin. Besides the transient and diffuse maculo-papules (roseola syphilitica), secondary syphilis skin lesions may have different morphology and shape. It has already been reported that in cases of reinfections by Treponema pallidum the skin lesions may be arciform, as in our patient, and asymmetric. Indeed, previous syphilis may attenuate the clinical and laboratory manifestations of a new infection with T.pallidum, that occurs with less severe skin manifestations or presents as latent syphilis. However, to our knowledge, annular-arciform lesions of the palms as unique manifestations of secondary syphilis have never been described. Clinicians should be well trained to recognize the atypical presentations of syphilis, especially in patients who have already received a syphilis diagnosis, since its incidence is steadily increasing in Europe, especially in men having sex with men.

scholarly journals A Case of Secondary Syphilis with the Extragenital Chancre on the Nipple

2020 ◽  
Vol 2020 ◽  
pp. 1-3
Author(s):  
Xiao Yan Wang ◽  
Juliandri ◽  
Zi Jing Liu ◽  
Jia Wen Zhang ◽  
Yun Yi Liu ◽  
...  
Keyword(s):  
Skin Biopsy ◽  
Sexually Transmitted Disease ◽  
Transmitted Disease ◽  
Treponema Pallidum ◽  
Signs And Symptoms ◽  
Secondary Syphilis ◽  
Primary Stage ◽  
Sexually Transmitted ◽  
Serological Examination

Syphilis is a sexually transmitted disease caused by Treponema pallidum. The signs and symptoms of syphilis vary depending on which of the four stages it presents. The primary stage of syphilis classically presents with a painless ulcer (chancre). We report a case of the extragenital chancre on the nipple which is examined from skin biopsy and immunohistochemistry. This case showed that it is important to identify the special site’s pruritus erythema by pathology and serological examination.

scholarly journals Edukasi Dokter pada Pasien Sifilis sebagai Upaya Pencegahan dan Pengobatan

2019 ◽  
Author(s):  
Andre Setiawan
Keyword(s):  
Sexually Transmitted Diseases ◽  
Early Stage ◽  
Transmitted Disease ◽  
Treponema Pallidum ◽  
Entire Body ◽  
Sexually Transmitted ◽  
Obligate Intracellular ◽  
Tertiary Syphilis ◽  
Advanced Stages ◽  
Latent Syphilis

Abstract. Syphilis is one of the STDs (Sexually Transmitted Diseases) that causes quite severe conditions for the sufferer. Caused by the bacterium Treponema pallidum subspecies pallidum (commonly called Treponema Pallidum) which is spiral in shape. Bacteria that are harmful to humans which are obligate intracellular, microaerophilic parasites, cannot live if they are rich in oxygen, antiseptic, soap and high temperatures. Syphilis is chronic, systemic, can infect the entire body even to the fetus of a pregnant woman. Syphilis is the most transmitted disease through sexual intercourse. Syphilis is clarified in several ways, one of which is based on epidemiology, divided into early and advanced stages. Early stage is more infectious compared to the advanced stage. Early-stage syphilis is divided into primary, secondary and latent syphilis. Advanced syphilis includes tertiary syphilis (gumatous, cardiovascular syphilis, neurosyphilis) and advanced latent syphilis. The purpose of this study is to prevent and treat syphilis patients.

scholarly journals Long-TermIn VitroCulture of the Syphilis SpirocheteTreponema pallidumsubsp.pallidum

mBio ◽  
2018 ◽  
Vol 9 (3) ◽  
Author(s):  
Diane G. Edmondson ◽  
Bo Hu ◽  
Steven J. Norris
Keyword(s):  
Transmitted Disease ◽  
Treponema Pallidum ◽  
Test Tube ◽  
Continuous Growth ◽  
Content Type ◽  
Sexually Transmitted ◽  
Treatment And Prevention ◽  
New Information

ABSTRACTInvestigation ofTreponema pallidumsubsp.pallidum, the spirochete that causes syphilis, has been hindered by an inability to culture the organism continuouslyin vitrodespite more than a century of effort. In this study, long-term logarithmic multiplication ofT. pallidumwas attained through subculture every 6 to 7 days and periodic feeding using a modified medium (T. pallidumculture medium 2 [TpCM-2]) with a previously described microaerobic, rabbit epithelial cell coincubation system. Currently, cultures have maintained continuous growth for over 6 months with full retention of viability as measured by motility and rabbit infectivity. This system has been applied successfully to the well-studied Nichols strain ofT. pallidum, as well as to two recent syphilis isolates, UW231B and UW249B. Light microscopy and cryo-electron microscopy showed thatin vitro-culturedT. pallidumretains wild-type morphology. Further refinement of this long-term subculture system is expected to facilitate study of the physiological, genetic, pathological, immunologic, and antimicrobial susceptibility properties ofT. pallidumsubsp.pallidumand closely related pathogenicTreponemaspecies and subspecies.IMPORTANCESyphilis, a sexually transmitted disease with a global distribution, is caused by a spiral-shaped bacterium calledTreponema pallidumsubspeciespallidum. Previously,T. pallidumwas one of the few major bacterial pathogens that had not been cultured long-termin vitro(in a test tube), greatly hindering efforts to better understand this organism and the disease that it causes. In this article, we report the successful long-term cultivation ofT. pallidumin a tissue culture system, a finding that is likely to enhance our ability to obtain new information applicable to the diagnosis, treatment, and prevention of syphilis.

scholarly journals Analytical performance characteristics of a new transcription-mediated amplification assay for Treponema pallidum

2021 ◽  
Author(s):  
Damon Getman ◽  
Mike Lin ◽  
Nesreen Barakat ◽  
Rhonda Skvoretz ◽  
Charmie Godornes ◽  
...  
Keyword(s):  
Diagnostic Testing ◽  
Transmitted Disease ◽  
Treponema Pallidum ◽  
Dark Field ◽  
Secondary Syphilis ◽  
Performance Characteristics ◽  
Specific Method ◽  
Analytical Sensitivity ◽  
Sexually Transmitted ◽  
New Research

This study evaluated the performance characteristics of a new research use transcription-mediated amplification (TMA) assay for detection of ribosomal RNA from Treponema pallidum (Tp). Analytical sensitivity determined using dark field quantitated Tp was 1.4 organisms/ml (95% CI: 0.7-6.33). Dilution of Tp IVT RNA in STM yielded 100% positivity (n=3) at 10 copies/ml (4 copies/reaction). Analytical specificity testing of non-target microorganisms (N=59), including the closely related non-syphilis treponemes T. denticola and T. phagedenis, yielded 0% positivity. TMA testing of mucosal swab specimens collected from men who have sex with men (MSM) attending a sexually transmitted disease clinic yielded 1.8% (17/944) TMA positive results. A collection of 56 serum specimens obtained from a separate cohort of patients with known rapid plasma reagin (RPR) status and syphilis clinical diagnosis were 19.6% (11/56) TMA positive overall, 29.7% (11/37) positive among subjects with syphilis diagnosis, including 8 (36.3%) of 22 persons with primary or secondary syphilis, 2 (20%) of 10 persons with early latent syphilis and 1 (20%) of 5 persons with late latent or unstaged syphilis. None (0%) of 18 RPR positive sera from patients with a history of treated syphilis was TMA positive. These results show TMA is an analytically sensitive and specific method for detection of Tp rRNA and is compatible with serum specimens in addition to pharyngeal and rectal mucocutaneous swab specimens. Automated real time TMA testing for T. pallidum may be useful as an adjunctive method with serology for screening and diagnostic testing of selected patient populations for syphilis.

Sign in / Sign up

Export Citation Format

Share Document