scholarly journals Comparison of direct immunofluorescence, conventional cell culture and polymerase chain reaction techniques for detecting respiratory syncytial virus in nasopharyngeal aspirates from infants

2008 ◽  
Vol 50 (1) ◽  
pp. 37-40 ◽  
Author(s):  
Alexanda Dias Reis ◽  
Maria Cristina Domingues Fink ◽  
Clarisse Martins Machado ◽  
José de Paula Paz Jr. ◽  
Renato Reis Oliveira ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Direct Immunofluorescence ◽  
Nasopharyngeal Aspirate ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Viral Isolation ◽  
Respiratory Tract Illnesses ◽  
Polymerase Chain ◽  
Syncytial Virus ◽  
Conventional Cell

A total of 316 samples of nasopharyngeal aspirate from infants up to two years of age with acute respiratory-tract illnesses were processed for detection of respiratory syncytial virus (RSV) using three different techniques: viral isolation, direct immunofluorescence, and PCR. Of the samples, 36 (11.4%) were positive for RSV, considering the three techniques. PCR was the most sensitive technique, providing positive findings in 35/316 (11.1%) of the samples, followed by direct immunofluorescence (25/316, 7.9%) and viral isolation (20/315, 6.3%) (p < 0.001). A sample was positive by immunofluorescence and negative by PCR, and 11 (31.4%) were positive only by RT-PCR. We conclude that RT-PCR is more sensitive than IF and viral isolation to detect RSV in nasopharyngeal aspirate specimens in newborn and infants.

scholarly journals Application of RT-PCR for diagnosis of respiratory syncytial virus and human metapneumovirus infections in Bulgaria, 2006-7 and 2007-8

2009 ◽  
Vol 14 (23) ◽  
Author(s):  
S Pavlova ◽  
T Hadzhiolova ◽  
P Abadjieva ◽  
R Kotseva
Keyword(s):  
Respiratory Syncytial Virus ◽  
Respiratory Diseases ◽  
National Laboratory ◽  
Human Metapneumovirus ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Acute Respiratory Diseases ◽  
Polymerase Chain ◽  
Syncytial Virus

We describe here the results of respiratory syncytial virus (RSV) detection by reverse transcription polymerase chain reaction (RT-PCR) during two consecutive seasons, from December 2006 to February 2007 and from October 2007 to March 2008, performed in the National Laboratory of Influenza and Acute Respiratory Diseases, Bulgaria. A total number of 278 nasopharyngeal samples obtained from hospitalised children up to the age of five years were investigated for these two seasons. During the first season, the aetiological role of RSV was confirmed in 56 of 148 samples (37.8%) compared to 11 of 130 samples (8.5%) during the second season. Since the beginning of January 2008, RT-PCR for the detection of the recently identified human metapneumovirus (HMPV) has also been introduced in Bulgaria. This virus has been demonstrated as the aetiological agent in 13 out of 81 samples (16%) from children of the same age group. The use of RT-PCR allows the detection of a broader spectrum of viruses causing respiratory diseases, as well as better discrimination of the aetiological agents in clinically similar cases.

Detection of Genomic Sequences of Respiratory Syncytial Virus in Otitis Media with Effusion in Children

1992 ◽  
Vol 101 (10_suppl) ◽  
pp. 7-10 ◽  
Author(s):  
Yoshitaka Okamoto ◽  
Kazuo Kudo ◽  
Koji Shirotori ◽  
Misao Nakazawa ◽  
Eiko Ito ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Respiratory Syncytial Virus ◽  
Otitis Media ◽  
Otitis Media With Effusion ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Rsv Infection ◽  
Polymerase Chain ◽  
Syncytial Virus ◽  
Viral Sequences

The reverse transcriptase—polymerase chain reaction and the nested polymerase chain reaction were used for detection of respiratory syncytial virus (RSV) sequences in middle ear effusions collected from children with otitis media. Sequences of RSV were detected in 21 of 34 samples tested. These samples were collected during and/or after natural outbreaks of RSV infection in the community. In those patients from whose nasopharynges RSV was isolated, the viral sequences were highly detectable (75%) in the effusions. These observations suggest RSV as an important factor in the pathogenesis of otitis media with effusion.

scholarly journals Respiratory Pathogens in Children 1 Month to 5 Years of Age Presenting With Undifferentiated Acute Respiratory Distress in 2 District-Level Hospitals in Ghana

2018 ◽  
Vol 8 (4) ◽  
pp. 361-364
Author(s):  
Patrick T Wilson ◽  
Frank Baiden ◽  
Joshua C Brooks ◽  
Katie M Giessler ◽  
Gavin Apio ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Respiratory Syncytial Virus ◽  
Respiratory Distress ◽  
Polymerase Chain Reaction Assay ◽  
Rainy Season ◽  
Dry Season ◽  
Respiratory Pathogens ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Syncytial Virus

Abstract Ghanaian children (2176) aged <5 years who presented with undifferentiated acute respiratory distress were tested for respiratory pathogens using a BioFire FilmArray polymerase chain reaction assay. Rhinovirus and/or enterovirus was detected in 36% of the assays, respiratory syncytial virus in 11%, and parainfluenza in 7%. Respiratory syncytial virus and metapneumovirus were detected more frequently in the rainy season than in the dry season.

scholarly journals Development of a Rapid Fluorescent Immunochromatographic Test to Detect Respiratory Syncytial Virus

2018 ◽  
Vol 19 (10) ◽  
pp. 3013 ◽  
Author(s):  
Trinh Thuy Tien ◽  
Hyun Park ◽  
Hien Tuong ◽  
Seung-Taek Yu ◽  
Du-Young Choi ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Rt Pcr ◽  
Immunochromatographic Strip ◽  
Copy Numbers ◽  
Rsv Infection ◽  
Immunochromatographic Strip Test ◽  
Polymerase Chain ◽  
Syncytial Virus ◽  
Strip Test ◽  
Europium Nanoparticles

Human respiratory syncytial virus (RSV) is one of the most common viruses infecting the respiratory tracts of infants. The rapid and sensitive detection of RSV is important to minimize the incidence of infection. In this study, novel monoclonal antibodies (mAbs; B11A5 and E8A11) against RSV nucleoprotein (NP) were developed and applied to develop a rapid fluorescent immunochromatographic strip test (FICT), employing europium nanoparticles as the fluorescent material. For the FICT, the limits of detection of the antigen and virus were 1.25 µg/mL and 4.23 × 106 TCID50/mL, respectively, corresponding to 4.75 × 106 ± 5.8 ×105 (mean ± SD) RNA copy numbers per reaction mixture for RSV NP. A clinical study revealed a sensitivity of 90% (18/20) and specificity of 98.18% (108/110) for RSV detection when comparing the performance to that of reverse transcription polymerase chain reaction (RT-PCR), representing a 15% improvement in sensitivity over the SD Bioline rapid kit. This newly developed FICT could be a useful tool for the rapid diagnosis of RSV infection.

One-step reverse transcriptase polymerase chain reaction for the diagnosis of respiratory syncytial virus in children

2008 ◽  
Vol 148 (1-2) ◽  
pp. 115-119 ◽  
Author(s):  
Cesar Augusto do Nascimento ◽  
Andréa Lima Leal ◽  
Thereza Silva Souza ◽  
Cláudia Trigo Pedroso de Moraes ◽  
Priscila Comone ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Respiratory Syncytial Virus ◽  
Reverse Transcriptase ◽  
Chain Reaction ◽  
One Step ◽  
Polymerase Chain ◽  
Syncytial Virus

scholarly journals Prevalence of respiratory viruses using polymerase chain reaction in children with wheezing, a systematic review and meta–analysis

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0243735
Author(s):  
Cyprien Kengne–Nde ◽  
Sebastien Kenmoe ◽  
Abdou Fatawou Modiyinji ◽  
Richard Njouom
Keyword(s):  
Systematic Review ◽  
Polymerase Chain Reaction ◽  
Respiratory Syncytial Virus ◽  
Molecular Detection ◽  
Respiratory Viruses ◽  
Human Bocavirus ◽  
Chain Reaction ◽  
Manual Search ◽  
Polymerase Chain ◽  
Syncytial Virus

Introduction Wheezing is a major problem in children, and respiratory viruses are often believed to be the causative agent. While molecular detection tools enable identification of respiratory viruses in wheezing children, it remains unclear if and how these viruses are associated with wheezing. The objective of this systematic review is to clarify the prevalence of different respiratory viruses in children with wheezing. Methods We performed an electronic in Pubmed and Global Index Medicus on 01 July 2019 and manual search. We performed search of studies that have detected common respiratory viruses in children ≤18 years with wheezing. We included only studies using polymerase chain reaction (PCR) assays. Study data were extracted and the quality of articles assessed. We conducted sensitivity, subgroup, publication bias, and heterogeneity analyses using a random effects model. Results The systematic review included 33 studies. Rhinovirus, with a prevalence of 35.6% (95% CI 24.6–47.3, I2 98.4%), and respiratory syncytial virus, at 31.0% (95% CI 19.9–43.3, I2 96.4%), were the most common viruses detected. The prevalence of other respiratory viruses was as follows: human bocavirus 8.1% (95% CI 5.3–11.3, I2 84.6%), human adenovirus 7.7% (95% CI 2.6–15.0, I2 91.0%), influenza virus6.5% (95% CI 2.2–12.6, I2 92.4%), human metapneumovirus5.8% (95% CI 3.4–8.8, I2 89.0%), enterovirus 4.3% (95% CI 0.1–12.9, I2 96.2%), human parainfluenza virus 3.8% (95% CI 1.5–6.9, I2 79.1%), and human coronavirus 2.2% (95% CI 0.6–4.4, I2 79.4%). Conclusions Our results suggest that rhinovirus and respiratory syncytial virus may contribute to the etiology of wheezing in children. While the clinical implications of molecular detection of respiratory viruses remains an interesting question, this study helps to illuminate the potential of role respiratory viruses in pediatric wheezing. Review registration PROSPERO, CRD42018115128.

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