scholarly journals Embryonic development of Aedes aegypti (Diptera: Culicidae): influence of different constant temperatures

2009 ◽  
Vol 104 (1) ◽  
pp. 124-126 ◽  
Author(s):  
Luana Cristina Farnesi ◽  
Ademir Jesus Martins ◽  
Denise Valle ◽  
Gustavo Lazzaro Rezende
Keyword(s):  
Embryonic Development ◽  
Aedes Aegypti

scholarly journals Aedes aegypti: egg morphology and embryonic development

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Ana Paula Miranda Mundim-Pombo ◽  
Hianka Jasmyne Costa de Carvalho ◽  
Rafaela Rodrigues Ribeiro ◽  
Marisol León ◽  
Durvanei Augusto Maria ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Aedes Aegypti ◽  
Vector Competence ◽  
Intermediate Stage ◽  
Egg Laying ◽  
Control Measures ◽  
Limiting Factor ◽  
Embryonic Cells ◽  
Biomedical Sciences ◽  
Egg Morphology

Abstract Background The diseases for which Aedes aegypti is a vector are worrisome. The high vector competence of this mosquito, as well as its anthropophilia and ability to adapt to the urban environment, allows it to exploit many habitats, making its prevention an arduous task. Despite current disease control measures focused on the mosquito, the effectiveness in containing its dispersion still requires improvement; thus greater knowledge about this insect is fundamental. Methods Aedes aegypti egg morphology and embryonic development were analyzed from eggs of the insectary of the Institute of Biomedical Sciences of the University of São Paulo. Optical (light and confocal) and electronic (transmission and scanning) microscopy were used to analyze the morphological and ultrastructural features of the eggs. Embryos were observed in the initial (0–20.5 h after egg-laying), intermediate (20.6–40.1 h after egg-laying), and final (40.2–61.6 h) stages of development, and kept at a temperature of 28 °C ± 1 °C until collection for processing. Results Eggs of Ae. aegypti were whitish at the time of oviposition, and then quickly became black. The egg length was 581.45 ± 39.73 μm and the width was 175.36 ± 11.59. Access to the embryo was difficult due to the egg morphology, point of embryonic development, and difficult permeability of the exochorion (mainly in fixation). Only about 5% of the collected eggs were successfully processed. In the initial stage of embryonic development, characteristics suggestive of intense cellular activity were found. In the intermediate stage, the beginning of the segmentation process was evident. In the final phase, it was possible to differentiate the cephalic region and the thoracic and abdominal segments. Conclusion The chorion was found to be an important protective barrier and a limiting factor for the evaluation of the embryos and mosquito embryonic cells, indicating that further studies need to be carried out to identify the reason that this occurs. Graphical abstract

scholarly journals Imidacloprid impairs the post-embryonic development of the midgut in the yellow fever mosquitoStegomyia aegypti(=Aedes aegypti)

2015 ◽  
Vol 29 (3) ◽  
pp. 245-254 ◽  
Author(s):  
K. M. FERNANDES ◽  
W. G. GONZAGA ◽  
T. V. PASCINI ◽  
F. R. MIRANDA ◽  
H. V. V. TOMÉ ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Aedes Aegypti ◽  
Yellow Fever

scholarly journals Quiescence in Aedes aegypti: Interpopulation Differences Contribute to Population Dynamics and Vectorial Capacity

Insects ◽  
2018 ◽  
Vol 9 (3) ◽  
pp. 111 ◽  
Author(s):  
Luciana Oliva ◽  
Roseli La Corte ◽  
Marcelo Santana ◽  
Cleide Albuquerque
Keyword(s):  
Population Dynamics ◽  
Embryonic Development ◽  
Aedes Aegypti ◽  
Development Time ◽  
Mosquito Species ◽  
Vectorial Capacity ◽  
Control Measures ◽  
Adult Size ◽  
Egg Viability ◽  
Male Predominance

The strategy of Aedes aegypti to prolong embryonic viability by quiescence has severe implications for geographic expansion and maintenance of mosquito populations in areas under control measures. We evaluated the effects of quiescence on biological parameters directly or indirectly associated with population dynamics and vectorial capacity in populations of this mosquito species from two Brazilian municipalities characterized as dengue, chikungunya, and Zika transmission areas. Egg viability, initial hatching time, post-embryonic development time, adult emergence rate, sexual proportion, adult size, fecundity, and fertility were analyzed using eggs stored for 10, 40, 70, 100, 130, and 160 d. Quiescence time reduced overall egg viability and post-embryonic development time in both municipalities but was more costly in Aracaju (100 d, 8 d) than in Recife (130 d, 7.5 d). Emergence rates increased in Recife when the eggs were older, but not in Aracaju. Significant deviations in sexual proportion, with male predominance, were observed in both populations. Initial hatch, fecundity, fertility, and adult size did not significantly influence egg quiescence time. These results indicate intrinsic and differential characteristics for each A. aegypti population, suggesting a differential cost of quiescence for population dynamics parameters that can indirectly affect vectorial capacity and control measures.

scholarly journals Proteomic analysis of Aedes aegypti midgut during post-embryonic development and of the female mosquitoes fed different diets

2016 ◽  
Vol 65 (6) ◽  
pp. 668-676 ◽  
Author(s):  
Kenner Morais Fernandes ◽  
Marcos Jorge de Magalhães-Júnior ◽  
Maria Cristina Baracat-Pereira ◽  
Gustavo Ferreira Martins
Keyword(s):  
Embryonic Development ◽  
Aedes Aegypti ◽  
Proteomic Analysis

scholarly journals An ancient satellite repeat controls gene expression and embryonic development in Aedes aegypti through a highly conserved piRNA

2020 ◽  
Author(s):  
Rebecca Halbach ◽  
Pascal Miesen ◽  
Joep Joosten ◽  
Ezgi Taşköprü ◽  
Bas Pennings ◽  
...  
Keyword(s):  
Gene Expression ◽  
Embryonic Development ◽  
Aedes Aegypti ◽  
Gene Silencing ◽  
Tandem Repeat ◽  
Satellite Repeat ◽  
Central Function ◽  
Satellite Repeats ◽  
In Trans ◽  
Regulatory Potential

AbstractTandem repeat elements such as the highly diverse class of satellite repeats occupy large parts of eukaryotic chromosomes. Most occur at (peri)centromeric and (sub)telomeric regions and have been implicated in chromosome organization, stabilization, and segregation1. Others are located more dispersed throughout the genome, but their functions remained largely enigmatic. Satellite repeats in euchromatic regions were hypothesized to regulate gene expression in cis by modulation of the local heterochromatin, or in trans via repeat-derived transcripts2,3. Yet, due to a lack of experimental models, gene regulatory potential of satellite repeats remains largely unexplored. Here we show that, in the vector mosquito Aedes aegypti, a satellite repeat promotes sequence-specific gene silencing via the expression of two abundant PIWI-interacting RNAs (piRNAs). Strikingly, whereas satellite repeats and piRNA sequences generally evolve extremely fast4-6, this locus was conserved for approximately 200 million years, suggesting a central function in mosquito biology. Tandem repeat-derived piRNA production commenced shortly after egg-laying and inactivation of the most abundant of the two piRNAs in early embryos resulted in an arrest of embryonic development. Transcriptional profiling in these embryos revealed the failure to degrade maternally provided transcripts that are normally cleared during maternal-to-zygotic transition. Our results reveal a novel mechanism in which satellite repeats regulate global gene expression in trans via piRNA-mediated gene silencing, which is fundamental to embryonic development. These findings highlight the regulatory potential of this enigmatic class of repeats.

scholarly journals Embryonic development and egg viability of wMel-infected Aedes aegypti

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Luana Cristina Farnesi ◽  
Thiago Affonso Belinato ◽  
João Silveira Moledo Gesto ◽  
Ademir Jesus Martins ◽  
Rafaela Vieira Bruno ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Aedes Aegypti ◽  
Egg Viability

Aedes Aegypti: Morphology the Egg and Embryonic Development

2021 ◽  
Author(s):  
Ana Paula Miranda Mundim-Pombo ◽  
Marisol León ◽  
Hianka Jasmyne Costa de Carvalho ◽  
Durvanei Augusto Maria ◽  
Maria Angelica Miglino
Keyword(s):  
Embryonic Development ◽  
Aedes Aegypti ◽  
Morphological Characteristics ◽  
Microscopic Analysis ◽  
Biomedical Sciences ◽  
Great Resistance ◽  
Egg Morphology ◽  
Great Relevance ◽  
Control Technologies ◽  
The University

Abstract Background: Aedes aegypti (Diptera: Culicidae) has great relevance in public health worldwide due to its performance as a vector of arboviruses, which makes the knowledge about its cycle fundamental. It is known that the acquaintance of embryonic kinetics has great potential to assist in the development of new vector control technologies and, consequently, in the control of arboviruses. Methods: This research proposes to analyze the egg morphology and embryonic development of Aedes aegypti. Eggs from the insectary of the Institute of Biomedical Sciences of the University of São Paulo (n = 46) were used. The methodological procedure involved morphological and ultrastructural analyzes using equipment for optical microscopy (light and confocal) and electronic (scanning). Eggs and embryos were observed in initial, intermediate and final thirds of development, kept at a temperature of 28°C, 1°C until collection for processing. Results: The embryos had different morphological characteristics according to the stage of gestation, in the initial and intermediate stages of development. In the initial third, the presence of primordial epithelium and characteristics suggestive of intense cellular activity were found. Conclusion: According to the results obtained in the eggs, it is concluded that the great resistance of the chorion in the embryo is a factor that generates difficulties for microscopic analysis of the embryo (mainly in fixation), with a strong protective barrier.

Live Confocal Analysis of Fertilization and Early Development

2001 ◽  
Vol 7 (S2) ◽  
pp. 1012-1013
Author(s):  
Uyen Tram ◽  
William Sullivan
Keyword(s):  
Drosophila Melanogaster ◽  
Embryonic Development ◽  
Real Time ◽  
Early Development ◽  
Fluorescent Probes ◽  
Primary Defect ◽  
Fluorescent Analysis ◽  
Dynamic Event ◽  
Enormous Amount ◽  
Fluorescent Studies

Embryonic development is a dynamic event and is best studied in live animals in real time. Much of our knowledge of the early events of embryogenesis, however, comes from immunofluourescent analysis of fixed embryos. While these studies provide an enormous amount of information about the organization of different structures during development, they can give only a static glimpse of a very dynamic event. More recently real-time fluorescent studies of living embryos have become much more routine and have given new insights to how different structures and organelles (chromosomes, centrosomes, cytoskeleton, etc.) are coordinately regulated. This is in large part due to the development of commercially available fluorescent probes, GFP technology, and newly developed sensitive fluorescent microscopes. For example, live confocal fluorescent analysis proved essential in determining the primary defect in mutations that disrupt early nuclear divisions in Drosophila melanogaster. For organisms in which GPF transgenics is not available, fluorescent probes that label DNA, microtubules, and actin are available for microinjection.

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