Aedes Aegypti: Morphology the Egg and Embryonic Development

2021 ◽  
Author(s):  
Ana Paula Miranda Mundim-Pombo ◽  
Marisol León ◽  
Hianka Jasmyne Costa de Carvalho ◽  
Durvanei Augusto Maria ◽  
Maria Angelica Miglino
Keyword(s):  
Embryonic Development ◽  
Aedes Aegypti ◽  
Morphological Characteristics ◽  
Microscopic Analysis ◽  
Biomedical Sciences ◽  
Great Resistance ◽  
Egg Morphology ◽  
Great Relevance ◽  
Control Technologies ◽  
The University

Abstract Background: Aedes aegypti (Diptera: Culicidae) has great relevance in public health worldwide due to its performance as a vector of arboviruses, which makes the knowledge about its cycle fundamental. It is known that the acquaintance of embryonic kinetics has great potential to assist in the development of new vector control technologies and, consequently, in the control of arboviruses. Methods: This research proposes to analyze the egg morphology and embryonic development of Aedes aegypti. Eggs from the insectary of the Institute of Biomedical Sciences of the University of São Paulo (n = 46) were used. The methodological procedure involved morphological and ultrastructural analyzes using equipment for optical microscopy (light and confocal) and electronic (scanning). Eggs and embryos were observed in initial, intermediate and final thirds of development, kept at a temperature of 28°C, 1°C until collection for processing. Results: The embryos had different morphological characteristics according to the stage of gestation, in the initial and intermediate stages of development. In the initial third, the presence of primordial epithelium and characteristics suggestive of intense cellular activity were found. Conclusion: According to the results obtained in the eggs, it is concluded that the great resistance of the chorion in the embryo is a factor that generates difficulties for microscopic analysis of the embryo (mainly in fixation), with a strong protective barrier.

scholarly journals Aedes aegypti: egg morphology and embryonic development

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Ana Paula Miranda Mundim-Pombo ◽  
Hianka Jasmyne Costa de Carvalho ◽  
Rafaela Rodrigues Ribeiro ◽  
Marisol León ◽  
Durvanei Augusto Maria ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Aedes Aegypti ◽  
Vector Competence ◽  
Intermediate Stage ◽  
Egg Laying ◽  
Control Measures ◽  
Limiting Factor ◽  
Embryonic Cells ◽  
Biomedical Sciences ◽  
Egg Morphology

Abstract Background The diseases for which Aedes aegypti is a vector are worrisome. The high vector competence of this mosquito, as well as its anthropophilia and ability to adapt to the urban environment, allows it to exploit many habitats, making its prevention an arduous task. Despite current disease control measures focused on the mosquito, the effectiveness in containing its dispersion still requires improvement; thus greater knowledge about this insect is fundamental. Methods Aedes aegypti egg morphology and embryonic development were analyzed from eggs of the insectary of the Institute of Biomedical Sciences of the University of São Paulo. Optical (light and confocal) and electronic (transmission and scanning) microscopy were used to analyze the morphological and ultrastructural features of the eggs. Embryos were observed in the initial (0–20.5 h after egg-laying), intermediate (20.6–40.1 h after egg-laying), and final (40.2–61.6 h) stages of development, and kept at a temperature of 28 °C ± 1 °C until collection for processing. Results Eggs of Ae. aegypti were whitish at the time of oviposition, and then quickly became black. The egg length was 581.45 ± 39.73 μm and the width was 175.36 ± 11.59. Access to the embryo was difficult due to the egg morphology, point of embryonic development, and difficult permeability of the exochorion (mainly in fixation). Only about 5% of the collected eggs were successfully processed. In the initial stage of embryonic development, characteristics suggestive of intense cellular activity were found. In the intermediate stage, the beginning of the segmentation process was evident. In the final phase, it was possible to differentiate the cephalic region and the thoracic and abdominal segments. Conclusion The chorion was found to be an important protective barrier and a limiting factor for the evaluation of the embryos and mosquito embryonic cells, indicating that further studies need to be carried out to identify the reason that this occurs. Graphical abstract

SEM and TEM observations of sperm development in the testis of the freshwater fish Oreochromis mossambicus

1990 ◽  
Vol 48 (3) ◽  
pp. 58-59
Author(s):  
Daryl A. Cornish ◽  
George L. Smit
Keyword(s):  
Morphological Characteristics ◽  
Oreochromis Mossambicus ◽  
Breeding Cycle ◽  
Limited Information ◽  
North West ◽  
The North ◽  
Sem And Tem ◽  
Sperm Development ◽  
Ultrastructural Characteristics ◽  
The University

Oreochromis mossambicus is currently receiving much attention as a candidater species for aquaculture programs within Southern Africa. This has stimulated interest in its breeding cycle as well as the morphological characteristics of the gonads. Limited information is available on SEM and TEM observations of the male gonads. It is known that the testis of O. mossambicus is a paired, intra-abdominal structure of the lobular type, although further details of its characteristics are not known. Current investigations have shown that spermatids reach full maturity some two months after the female becomes gravid. Throughout the year, the testes contain spermatids at various stages of development although spermiogenesis appears to be maximal during November when spawning occurs. This paper describes the morphological and ultrastructural characteristics of the testes and spermatids.Specimens of this fish were collected at Syferkuil Dam, 8 km north- west of the University of the North over a twelve month period, sacrificed and the testes excised.

scholarly journals Embryonic development of Aedes aegypti (Diptera: Culicidae): influence of different constant temperatures

2009 ◽  
Vol 104 (1) ◽  
pp. 124-126 ◽  
Author(s):  
Luana Cristina Farnesi ◽  
Ademir Jesus Martins ◽  
Denise Valle ◽  
Gustavo Lazzaro Rezende
Keyword(s):  
Embryonic Development ◽  
Aedes Aegypti

scholarly journals Teaching Research Integrity and Bioethics to Science Undergraduates

2005 ◽  
Vol 4 (4) ◽  
pp. 330-334 ◽  
Author(s):  
Julio F. Turrens
Keyword(s):  
Undergraduate Students ◽  
Research Integrity ◽  
National Institutes Of Health ◽  
Biomedical Sciences ◽  
Teaching Research ◽  
Office Of Research Integrity ◽  
The University

Undergraduate students in the Department of Biomedical Sciences at the University of South Alabama, Mobile, are required to take a course entitled“ Issues in Biomedical Sciences,” designed to increase students' awareness about bioethical questions and issues concerning research integrity. This paper describes the main features of this course and summarizes the results of a survey designed to evaluate the students' perceptions about the course. A summary of this study was presented at the 2002 Conference on Research Integrity in Potomac, MD, sponsored by the Office of Research Integrity of the National Institutes of Health.

scholarly journals Dark-chilling of Seedlings Affects Initiation and Morphology of Broccoli Inflorescence

2013 ◽  
Vol 41 (1) ◽  
pp. 213 ◽  
Author(s):  
Aneta GRABOWSKA ◽  
Agnieszka SĘKARA ◽  
Monika BIENIASZ ◽  
Edward KUNICKI ◽  
Andrzej KALISZ
Keyword(s):  
Microscopic Analysis ◽  
Flower Buds ◽  
Uniform Size ◽  
Sowing Time ◽  
Regular Form ◽  
Plant Ontogeny ◽  
Branching Angle ◽  
The University ◽  
And Control

The constant supply of broccoli heads for a vegetable market can be kept by the differentiation of sowing time and the age of seedlings. Dark-chilling of young seedlings can also modify the time of transplanting, subsequent plant development, yield and inflorescence quality. The aim of the present study was to analyze the influence of seedling dark-chilling on the inflorescence initiation and the morphological quality of broccoli heads. The experiment was carried out at the University of Agriculture in Krakow (South Poland) in the years 2009 and 2010, with broccoli (Brassica oleracea L. var. italica Plenck) medium late cultivar ‘Monaco’ F1. The examined factors were the age of seedlings (4-, 6-, 8-, and 10-week-old) and dark-chilling (2°C/2 weeks and non-chilled control). Microscopic analysis of the apex sections was performed to determine the time of the inflorescence differentiation in chilled and non-chilled seedlings of different age. Chosen morphological features of the mature heads were also described as depended on age and chilling of seedlings. The inflorescence initiation was observed between 4 and 6 week of the plant ontogeny. Especially 4-week-old seedlings, chilled by two weeks, were more advanced in the generative development in comparison to non-chilled control. Low temperature applied in the seedling stage did not cause the head buttoning. Inflorescences developed by broccoli planted from 4 and 6-week-old seedlings, both chilled and control, were characterized by the best visual quality, determined by a convex head shape, tight branching angle, small and uniform size and regular form of flower buds. Chilling of oldest, 8- and 10-week-old seedlings negatively affected the inflorescence quality, through planed and spread shape of heads and non-uniform shape of flower buds.

Time-lapse microscopic analysis to verify how blastomere biopsy for PGD affects the dynamics of embryonic development

2014 ◽  
Vol 102 (3) ◽  
pp. e19-e20 ◽  
Author(s):  
D. Ben-Yosef ◽  
L. Bar-El ◽  
T. Shwartz ◽  
T. Cohen ◽  
A. Carmon ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Microscopic Analysis ◽  
Time Lapse ◽  
Blastomere Biopsy

WHERE PATHOLOGY, MICROBIOLOGY AND VIROLOGY CONVERGE: PROFESSOR VICTOR BABEȘ

2021 ◽  
Vol 80 (2) ◽  
pp. 179-188
Author(s):  
Mircea Ioan Popa
Keyword(s):  
High School ◽  
Medical Education ◽  
World Literature ◽  
Biomedical Sciences ◽  
Medical Organization ◽  
New Horizons ◽  
Medical Needs ◽  
University Of Budapest ◽  
The University ◽  
Higher Medical Education

"Providential personalities are an essential, unifying, element across cultures, omnipresent in the world’s history [1]. Professor Victor Babeș, a distinguished figure in pathology, microbiology and virology, attended high school in Budapest and pursued his higher medical education in Budapest and Wien. He lectures at the pathology department of the University of Budapest at only 18 years of age. In 1885, he co-authored the first Bacteriology Treaty in Paris alongside Cornil. In 1887, he went on to establish the pathology and bacteriology departments at the Faculty of Medicine, in Bucharest. He contributed to the medical world literature with over 1000 papers, written in different languages. Professor Victor Babeș studied bacteria, viruses, parasites and influenced preventive medicine. Overall, he discovered more than 50 new microbes. An entire book may be devoted just to his pathological discoveries (cancer, leprosy, tuberculosis, diphtheria, actinomycosis, anthrax, syphilis, plague, rabies, etc.). In 1912 he published (in Paris) a vast treaty on rabies; he dedicated 90 of his best works to rabies. Victor Babeș National Institute of Research and Development in Pathology and Biomedical Sciences has been founded on the 28th of April 1887 by Professor Victor Babeș. Initially constituted as a “Bacteriology and Pathology Institute”, it was able to comply with one of the most demanding medical needs of the time and it has contributed to finding realistic solutions regarding medical organization. In terms of importance and activity, the institute represented a prefiguration of the future Ministry of Health [2]. Professor Babeș's life should be remembered for his works that marked the opening of new horizons and for a personality whose genius echoes to this day. "

scholarly journals Risk of transmission of viral haemorrhagic fevers and the insecticide susceptibility status of aedes aegypti (linnaeus) in some sites in Accra, Ghana

2016 ◽  
Vol 50 (3) ◽  
pp. 136-141
Author(s):  
Takashi Suzuki ◽  
Joseph H Osei ◽  
Sasaki Sasaki ◽  
Michelle Adimazoya ◽  
Maxwell Appawu ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Emerging Diseases ◽  
Research Network ◽  
Insecticide Susceptibility ◽  
Aedes Mosquitoes ◽  
Human Landing ◽  
Survey Results ◽  
The University ◽  
University Of Ghana ◽  
Susceptibility Status

Background: Dengue is one of the emerging diseases that can mostly only be controlled by vector control since there is no vaccine for the disease. Although, Dengue has not been reported in Ghana, movement of people from neighbouring countries where the disease has been reported can facilitate transmission of the disease. Objective: This study was carried on the University of Ghana campus to determine the risk of transmission of viral haemorrhagic fevers and the insecticide susceptibility status of Ae. aegypti in some sites in Accra, Ghana. Design: Larval surveys were carried to inspect containers within households and estimate larval indices and adult Aedes mosquitoes were collected using human landing collection technique. WHO tube assays was used to assess the insecticide susceptibility status of Aedes mosquitoes. Results: Ae. aegypti were the most prevalent species, 75.5% and followed by Ae. vittatus, 23.9 %. Ae. albopictus and Ae. granti were in smaller numbers. Household index (HI), Breteau index (BI), and container index were calculated as 8.2%, 11.2% and 10.3% respectively with man-vector contact rate of 0.67 bites/man-hour estimated for the area. The mortalities recorded for Ae. aegypti from WHO tube assays was 88%, 94%, 80% and 99% for DDT (4%), deltamethrin (0.05%), lambdacyhalothrin (0.05%) and permethrin (0.75%) respectively.Conclusion: The survey results indicated that the density of Aedes mosquitoes was considered to be sufficient to promote an outbreak of viral haemorrhagic fevers on Legon Campus. Aedes mosquitoes were found to be resistant to DDT, deltamethrin and lamdacyhalothrin, but susceptible to permethrin. Funding: This study was supported in part by Japan Initiative for Global Research Network on Infectious Diseases(J-Grid).Keywords: Mosquitoes, Aedes aegypti, insecticide, risk, VHF transmission, Ghana

The development and implementation of a new medical biology major including physiology

2015 ◽  
Vol 39 (2) ◽  
pp. 67-75 ◽  
Author(s):  
Barbara E. Goodman ◽  
Karen L. Koster ◽  
David L. Swanson
Keyword(s):  
South Dakota ◽  
Ad Hoc ◽  
Doctoral Programs ◽  
Howard Hughes Medical Institute ◽  
Medical Institute ◽  
Biomedical Sciences ◽  
Medical College ◽  
Curriculum Review ◽  
The University ◽  
Science Departments

In response to the Howard Hughes Medical Institute/Association of American Medical Colleges Scientific Foundations for Future Physicians (SFFP) report and a concern for better preparing undergraduates for future doctoral programs in the health professions, the deans of the College of Arts and Sciences and Division of Basic Biomedical Sciences of Sanford School of Medicine of the University of South Dakota formed an ad hoc Premedical Curriculum Review Committee with representatives from the science departments and medical school. The Committee began by reviewing the university's suggested premedical curriculum and matching it to the proposed competencies from the SFFP to document duplications and deficiencies. The proposed changes in the Medical College Admission Test for 2015 were also evaluated. The Committee proposed a stronger premedical curriculum, with the development of some new courses, including an inquiry-based physiology course with team-based learning, to more fully address SFFP competencies. These analyses convinced the university that a new major would best help students achieve the competencies and prepare them for admission exams. Thus, a new Medical Biology major was proposed to the South Dakota Board of Regents and accepted for its initial offering in 2012. The new major has been broadly advertised to future students and is successful as a recruiting tool for the university. This article details the process of evaluating the curriculum and designing the new major, describes some of the difficulties in its implementation, and reviews outcomes from the new major to date.

Sign in / Sign up

Export Citation Format

Share Document