scholarly journals Designs and model effects definitions in the initial stage of a plant breeding program

2006 ◽  
Vol 41 (3) ◽  
pp. 369-375 ◽  
Author(s):  
Emanuel Fernando Maia de Souza ◽  
Luiz Alexandre Peternelli ◽  
Márcio Henrique Pereira Barbosa

The objective of this work was to compare the relative efficiency of initial selection and genetic parameter estimation, using augmented blocks design (ABD), augmented blocks twice replicated design (DABD) and group of randomised block design experiments with common treatments (ERBCT), by simulations, considering fixed effect model and mixed model with regular treatment effects as random. For the simulations, eight different conditions (scenarios) were considered. From the 600 simulations in each scenario, the mean percentage selection coincidence, the Pearsons´s correlation estimates between adjusted means for the fixed effects model, and the heritability estimates for the mixed model were evaluated. DABD and ERBCT were very similar in their comparisons and slightly superior to ABD. Considering the initial stages of selection in a plant breeding program, ABD is a good alternative for selecting superior genotypes, although none of the designs had been effective to estimate heritability in all the different scenarios evaluated.

2018 ◽  
Vol 10 (1) ◽  
pp. 153-159
Author(s):  
Rohma Istiana ◽  
Hermin Pancasakti Kusumaningrum ◽  
Rejeki Siti Ferniah

The identification and the characterization of genetic diversity of rice was the first step in the rice plant breeding program. This study aimed to detect indica or japonica sub-species on upland rice Situ Bagendit and inbred rice Ciherang using molecular markers ORF 29-TrnC (GCA) on the chloroplast genome. Rice was included to the indica sub-species if the 32 bp insertion on ORF 29-TrnC (GCA) sequence was found, on the contrary, if the deletion 32 bp on ORF 29-TrnC (GCA) was found then it was included to the japonica sub-species. DNA isolation was examined from the leaves of the rice plants, and then it tested quantitatively to determine the transparency and DNA concentration from the isolation results. PCR amplification was performed using a pair of primers CP2 and it was followed by agarose gel electrophoresis. The visualization of the DNA bands used the gel documentation. Sequencing of PCR products produced a long base 390 bp in Situ Bagendit rice and 390 bp in Ciherang rice. Analysis of the sequences showed that the insertions occurred throughout the 32 bp in Situ Bagendit rice and the insertions occurred throughout the 32 bp in Ciherang rice. The results showed that upland rice Situ Bagendit and inbred rice Ciherang were included in the indica sub-species. The knowledge of variety of genetics of rice can be used as bio-information in the plant breeding program. Further, the knowledge can be used to protect in genetic power source, the selection and the composing of superior varieties of rice which is tolerant with kinds of biotic and abiotic factor.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 225-226
Author(s):  
María A Vulliez ◽  
María Bruni ◽  
Ana I Trujillo ◽  
Martín Durante ◽  
Lucrecia Lezana ◽  
...  

Abstract The functional types of plants (PFT) ecological approach could be used to link the preferences of ruminants. In order to investigate if ruminant preferences were associated with this approach, three PFT groups were evaluated. A group “B”, represented by Paspalum urvillei (PU-B), “C” by Nassella neesiana (NN-C) and Sporobolus indicus (SI-C) and “D” by Andropogon lateralis (AL-D). In a randomized block design with repeated measures over time, two independent cafeteria trials (autumn-winter, A-W, and spring-summer, S-S) were carried out in calves (n = 8 for A-W; n = 4 for S-S; BW=240–270 kg) arranged in four pens. In three sessions of ten minutes each, calves received 120–160 gDM of fresh cut leaves from the different species. Dry matter intake (DMI), nitrogen intake (NI) and neutral detergent fiber intake (NDFI) were measured. Data were analyzed with a mixed model that included specie and session as fixed effects and blocks as a random. The statistical analyses were performed with SAS (University Edition), data is expressed as lsmeans and was considered to differ when Tukey test presented P < 0.05. No significant differences were observed between sessions. The DMI and NI were higher in NN-C and lower in AL-D (P < 0.01) during A-W. However, in S-S, higher DMI and NI were observed in AL-D and SI-C while NN-C presented the lower DMI and NI (P < 0.01). The FDNI did not show differences in A-W meanwhile in S-S was positively associated with DMI (Table1). Interestingly, when the season changed, it was observed a change in PFT preference, in A-W calves preferred NN-C whereas in S-S preferred AL-D. However, attributes associated with preferences were maintained in both stations, for instance, higher DMI was associated with higher NI. Additional research is required on the interactions between PFT approach and the preference of animals for different species.


2016 ◽  
Vol 14 (2) ◽  
pp. 57 ◽  
Author(s):  
NFN Sumarno ◽  
Nani Zuraida

<p>Plant breeding, as an applied of plant genetics, is based and is supported by various subdisciplines of genetic sciences, includeing plant germplasm, classical genetics, molecular genetics, cytogenetics, gene-transformation techniques, etc. Linkage and team work system between plant germplasm management and plant breeding program is most required, since the success of plant breeding maybe obtained from the contribution of gene donor parents, derived from the germplasm management. Without the flow of genes from the germplasm collection, varieties produced by the plant breeder would suffer a narrow genetical based or a bottle-necking genetic based. Plant germplasm research is an integral part of the germplasm management, aimed to (1) evaluate the genetic variation of the germplasm collection, to be readily available for the breeding program and to be used for scientific publications, (2) tracing the origin of plant species, and (3) officially release a selected germplasm, containing new economic gene (s). The linkage between germplasm management and plant breeding research program could be facilitated through the following activities (1) identifying an elit germplasm for varietal release, (2) selection and stabilization of a promising germplasm accession for possible varietal releases, (3) use of germplasm accession as a gene donor parent to incorporate adaptive genes into improved variety, (4) use of germplasm accession for a specific donor gene, (5) use of germplasm to broaden the genetical base of varieties through an introgression and nobilization, (6) use of germplasm to improve the genetic value of the breeding population, and (7) to develop multiple crossess involving many parents to broaden the genetical base of the breeding population. Another important function of the germplasm management is to conserve accessions carrying genes which may be useful in the future, to anticipate the dynamic changing of biological and environmental stresses on crop. Germplasm management is considered successfully conducted when it is continously supplying donor gene parents to breeders for parental crosses on their breeding program, conversely, breeding program in considered successfully managed, when it uses the rich genetic variability available on the germplasm collection. Separating the organizational units among the breeding program, germplasm management and molecular genetic research, is only for enhancing the intensity of the research, but should not separate the linkage program of the research.</p><p> </p><p><strong>Abstrak</strong></p><p>Pemuliaan tanaman merupakan ilmu genetika terapan yang didukung oleh berbagai cabang ilmu kegenetikaan, termasuk plasma nutfah, genetika klasik, genetika molekuler, sitogenetika, dan genetika transformasi. Keterpaduan antara pengelolaan plasma nutfah dengan pemuliaan tanaman tidak dapat ditawar, karena keberhasilan pemuliaan sangat tergantung dari ketersediaan sumber gen yang disediakan oleh pengelola plasma nutfah. Tanpa kontribusi sumber gen dari pengelola plasma nutfah, hasil pemuliaan tanaman mengalami penyempitan kandungan genetik, atau terjadi gejala leher botol genetik. Penelitian plasma nutfah merupakan bagian integral dari pengelolaan materi plasma nutfah, bertujuan untuk (1) menggali kekayaan sifat genetik plasma nutfah guna penyediaan tetua persilangan dan bahan publikasi ilmiah, (2) menelusuri asal-usul spesies tanaman, (3) melepas secara resmi plasma nutfah sebagai sumber gen yang diakui kepemilikannya. Keterkaitan pengelolaan plasma nutfah dengan program pemuliaan dapat dilaksanakan melalui (1) pemanfaatan langsung aksesi plasma nutfah elit untuk dilepas sebagai varietas unggul, (2) pemurnian dan pemantapan populasi aksesi plasma nutfah sebagai calon varietas, (3) pemanfaatan aksesi plasma nutfah sebagai donor gen untuk rekombinasi gen-gen unggul adaptif, (4) plasma nutfah sebagai donor gen spesifik, (5) plasma nutfah sebagai bahan perluasan latar belakang genetik varietas melalui proses introgresi dan nobilisasi, (6) pemanfaatan plasma nutfah untuk perbaikan genetik populasi seleksi, dan (7) pembentukan populasi dasar yang mengandung keragaman genetik luas melalui persilangan banyak tetua. Fungsi pengelolaan plasma nutfah lainnya adalah melestarikan sumber daya genetik untuk kebutuhan gen di masa depan, agar dapat menyediakan gen-gen untuk mengantisipasi perubahan ras patogen dan tipe baru serangga hama yang bersifat dinamis, serta penyediaan gen guna mengatasi cekaman abiotik alamiah. Pengelolaan plasma nutfah dinilai berhasil apabila telah mampu menyediakan aksesi plasma nutfah sebagai sumber gen donor dalam program pemuliaan. Pemuliaan tanaman berhasil secara optimal apabila telah memanfaatkan keragaman genetik sifat yang diinginkan, yang tersedia dalam koleksi plasma nutfah. Keterpisahan kelembagaan antara unit kerja pengelolaan plasma nutfah dengan program pemuliaan tidak boleh membatasi keterpaduan program penelitian antara kedua cabang disiplin keilmuan tersebut.</p>


Author(s):  
Ismail Ibrahim Garba ◽  
Jibrin Mohammed Jibrin ◽  
Alpha Yaya Kamara ◽  
Adnan Aminu Adnan ◽  
Bassam Lawan Abdulrahman ◽  
...  

To close the nutrient-related yield gaps in maize, balanced nutrition using primary and secondary macronutrients as well as micronutrients is recommended. Multi-nutrient diagnostic field trials were conducted in Guinea Savanna of Nigeria to assess the interactive effects of macronutrients and micronutrients on maize grain yields, nutrient uptake as well as N, P and K use efficiencies. The treatments consisted of a control (zero fertilizer applied), an NPK treatment and 10 other treatments in which macronutrient (Mg, S) and/or micronutrients (B, and Zn) were added to the NPK. The experiment was laid out in Randomized Complete Block Design with 3 replications. Data collected were subjected to mixed model with nutrient management strategy as fixed effects while replication nested in location and interaction between location and nutrient management strategy as random effects. The results revealed significant effects (P &lt; 0.05) of nutrient management strategy on maize yield, nutrient uptake and nutrient use efficiencies of N, P and K. The study revealed that yield advantage over the recommended NPK fertilizer as a result of application macronutrients and micronutrients were highest with Mg in Lere (2.4 t ha&minus;1), S + B + Zn in Faskari (2.8 t ha&minus;1), S + B in Doguwa (1.5 t ha&minus;1) and S + Zn in Toro (2.4 t ha&minus;1).&nbsp; Addition of Mg, S and B significantly increases macronutrient uptakes over the recommended NPK only. Agronomic use efficiency, internal utilization efficiency, apparent recovery efficiency and partial factor productivity were significantly increased with the addition of S, Mg, and B but were not improved with Zn application. It was concluded that nutrient limitations to maize in the Guinea Savanna go beyond N, P and K. This study recommends that S, Mg, and B are needed to improve maize productivity and engender improve the use efficiency of NPK fertilizers.


2005 ◽  
Vol 114 (3) ◽  
pp. 177-182 ◽  
Author(s):  
Albert L. Merati ◽  
Seckin O. Ulualp ◽  
Hyun J. Lim ◽  
Robert J. Toohill

We report a meta-analysis of a series of studies in which 24-hour ambulatory pH monitoring was performed in 1) normal subjects, 2) the normal control subjects in studies of laryngopharyngeal reflux (LPR), and 3) the patients with LPR in these controlled studies. The statistical analysis utilized the fixed-effects model by Mantel-Haenszel and the random-effects mixed model. There were 16 studies from the past 12 years that fulfilled the inclusion criteria. They involved 793 subjects (264 normal and 529 with LPR). The numbers of positive pharyngeal reflux events for normal subjects and for patients with LPR differed with a p value of <.0001. There was also a significant difference in the mean percentage of acid exposure times between normal subjects and patients with LPR (p = .003). We conclude that the upper probe gives accurate and consistent information in normal subjects and patients with LPR. The numbers of reflux events and acid exposure times are most important in distinguishing normal subjects from patients with LPR. The technology and methodology of probe testing is quite reliable and is consistent on a worldwide basis.


1981 ◽  
Vol 30 (1-2) ◽  
pp. 57-64
Author(s):  
Sunanda Mukhopadhyay

For the problem of estimation of all elementary treatment contraata, restrictina ourselves to PBIBD's with two clus association schemea under a mixed model we follow the approach of Sinha and Sinha (1969) to study the behaviour of tho average variance [Formula: see text] as a function of [Formula: see text] and also as a function of the auociation scheme to some extent. The functional form of [Formula: see text] turna out to be the Jame as that under fixed effects model and either [Formula: see text] for all admissible θ>0. We got that when a BIB dosian does not exist with tho alven parameters a GO design with [Formula: see text] if it exists, is A-optimal within the class of all PBIB designs with two associate class association schemes. This proves that in respect of A-optimality, the result of Chong (1978) can be extended to all GD designs with [Formula: see text] if we restrict ourselves to such PBIB dosigns.


2021 ◽  
Vol 305 ◽  
pp. 03006
Author(s):  
Fetrina Oktavia ◽  
Sahuri ◽  
Dwi Shinta Agustina

The use of superior rubber planting materials is one of the most important components of technology to support the cultivation and sustainability of the natural rubber industry. The effect of the genetic components of planting materials to the rubber productivity can reach 60%, and the rest is the influence of agro-climatic conditions. The aim of the rubber plant breeding program is to obtain the new superior rubber clones that have a high latex yielding potential and good agronomic characters. The fluctuations of natural rubber price and climate changes also influence the direction and objectives of the rubber plant breeding program. To deal with the conditions, it is important to provide the rubber agroforestry technology by through intercropping of rubber with various other crops. The article will provide the information about progress of rubber breeding program in Indonesia and it is role in supporting agroforestry system. Several of new superior rubber clones have been released by IRRI, and some of these clones such as IRR 112 and IRR 118 had been planting with rice, corn, and other crops by through rubber agroforestry system. The system was estimated be able to maintain latex yielding potential of clones as well as farmers’ income can be improved.


2021 ◽  
Vol 2021 ◽  
pp. 1-16
Author(s):  
David M. Deery ◽  
Hamlyn G. Jones

Field phenomics has been identified as a promising enabling technology to assist plant breeders with the development of improved cultivars for farmers. Yet, despite much investment, there are few examples demonstrating the application of phenomics within a plant breeding program. We review recent progress in field phenomics and highlight the importance of targeting breeders’ needs, rather than perceived technology needs, through developing and enhancing partnerships between phenomics researchers and plant breeders.


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