scholarly journals In vitro maintenance, under slow-growth conditions, of oil palm germplasm obtained by embryo rescue

2015 ◽  
Vol 50 (5) ◽  
pp. 426-429 ◽  
Author(s):  
Julcéia Camillo ◽  
Jonny Everson Scherwinski-Pereira
Keyword(s):  
Plant Growth ◽  
Oil Palm ◽  
Culture Medium ◽  
Slow Growth ◽  
Elaeis Guineensis ◽  
Embryo Rescue ◽  
Growth Conditions ◽  
In Vitro Maintenance

The objective of this work was to evaluate the in vitro maintenance of oil palm (Elaeis guineensis and E. oleifera) accessions under slow-growth conditions. Plants produced by embryo rescue were subject to 1/2MS culture medium supplemented with the carbohydrates sucrose, mannitol, and sorbitol at 1, 2, and 3% under 20 and 25±2ºC. After 12 months, the temperature of 20°C reduced plant growth. Sucrose is the most appropriate carbohydrate for maintaining the quality of the plants, whereas mannitol and sorbitol result in a reduced plant survival.

scholarly journals Adaptability and leaf anatomical features in oil palm seedlings produced by embryo rescue and pre-germinated seeds

2010 ◽  
Vol 22 (3) ◽  
pp. 209-215 ◽  
Author(s):  
Zanderluce G. Luis ◽  
Kadja Milena G. Bezerra ◽  
Jonny Everson Scherwinski-Pereira
Keyword(s):  
Oil Palm ◽  
Embryo Rescue ◽  
Morphological Characteristics ◽  
Growth Conditions ◽  
Epidermal Cells ◽  
In Vitro Cultivation ◽  
Vascular Bundles ◽  
Anatomical Plasticity ◽  
Germinated Seeds

Changes in the leaf structure of plants grown in different conditions have been reported, such as increase in size and density of stomata and reduction in stomatal control, amount of epicuticular wax, and mesophyll thickness, with a high diversity of intercellular spaces. However, these changes are highly variable depending on the physiological and morphological characteristics of each species. The objective of this work was to analyze the adaptability and anatomical plasticity of oil palm seedlings produced after embryo rescue and pre-germinated seeds. Expanded leaves were prepared for evaluation of morphometric data and anatomical structures. It was verified that the environmental conditions in vitro negatively influenced the stomata density, epidermal and hypodermal thickness, and the values for the expansion cells and leaf mesophile. Anatomically, the oil palm leaves present the same tissues composition in both growth conditions, with uniseriate epidermal cells, and tetracitic stomata occurring in both epidermal surfaces. Epidermal cells from in vitro plants are thinner than ones from greenhouse. The midrib of leaves from greenhouse plants are more developed and is composed by only one central vascular bundle, while plants from in vitro cultivation developed three to four collateral vascular bundles.

scholarly journals In vitro embryo rescue of interspecific hybrids of oil palm (Elaeis oleifera x Elaeis guineensis)

2011 ◽  
Vol 2 (2) ◽  
pp. 1-6 ◽  
Author(s):  
Sergio Augusto Oliveira Alves ◽  
Oriel Filgueira de Lemos ◽  
Benedito Gomes dos Santos Filho ◽  
André Luís Lopes da Silva
Keyword(s):  
Oil Palm ◽  
Activated Charcoal ◽  
Interspecific Hybrids ◽  
Elaeis Guineensis ◽  
Germination Rate ◽  
Embryo Rescue ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Elaeis Oleifera

The oil palm (Elaeis guineennsis Jacq.) is the plant with major oil yield in tropical areas over the world. Pará state is the major producer of Brazil. However, the production of resistant hybrids (Elaeis oleifera x Elaeis guinennsis) to several diseases is necessary, in spite of less productive, is a viable alternative for areas where occur more aggressive diseases. The aim of this research was to rescue in vitro embryos of four varieties of interspecific hybrids of oil palm (Elaeis oleifera x Elaeis guineensis) in different media. Four varieties were tested: (1) Cj-2141; (2) CI-2061; (3) Cj-502 and (4) Cj-494. Four media were tested: (T1) half strength MS medium (½ MS), (T2) MS, (T3) MS + 0.5mg L-1 1-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP) and (T4) ½ MS+ 0.5mg.L-1 NAA and BAP. All media were supplemented with 0.17g.L-1 NaH2PO4, activated charcoal (0.25%), sucrose (3%) and solidified with phytagel (0.2%). The most germination rate of interspecific hybrids embryos was obtained on ½ MS medium, supplemented with 0.17g.L-1 NaH2PO4, activated charcoal (0.25%), sucrose (3%) and solidified with phytagel (0.2%). The variety CI-2061 presents the best capacity to develop normal seedlings.

Mature zygotic embryo rescue improves in vitro germination and seedling production in high value oil palm (Elaeis guineensis Jacq.) cultivars

2016 ◽  
Vol 94 ◽  
pp. 445-453 ◽  
Author(s):  
Kingsley Tabi Mbi ◽  
Libert Brice Tonfack ◽  
Godswill Ntsomboh Ntsefong ◽  
Bilal Ahmad Mir ◽  
Georges Frank Ngando Ebongue ◽  
...  
Keyword(s):  
Oil Palm ◽  
Elaeis Guineensis ◽  
Zygotic Embryo ◽  
Embryo Rescue ◽  
In Vitro Germination ◽  
Seedling Production ◽  
Elaeis Guineensis Jacq ◽  
Mature Zygotic Embryo

scholarly journals In vitro conservation of Piper aduncum and Piper hispidinervum under slow-growth conditions

2011 ◽  
Vol 46 (4) ◽  
pp. 384-389 ◽  
Author(s):  
Tatiane Loureiro da Silva ◽  
Jonny Everson Scherwinski-Pereira
Keyword(s):  
Low Temperatures ◽  
Culture Medium ◽  
Shoot Growth ◽  
Slow Growth ◽  
Growth Conditions ◽  
In Vitro Cultures ◽  
In Vitro Conservation ◽  
Piper Aduncum ◽  
Osmotic Agents

The objective of this work was to evaluate in vitro storage of Piper aduncum and P. hispidinervum under slow-growth conditions. Shoots were stored at low temperatures (10, 20 and 25°C), and the culture medium was supplemented with osmotic agents (sucrose and mannitol - at 1, 2 and 3%) and abiscisic acid - ABA (0, 0.5, 1.0, 2.0 and 3.0 mg L-1). After six-months of storage, shoots were evaluated for survival and regrowth. Low temperature at 20ºC was effective for the in vitro conservation of P. aduncum and P. hispidinervum shoots. In vitro cultures maintained at 20ºC on MS medium showed 100% survival with slow-growth shoots. The presence of mannitol or ABA, in the culture medium, negatively affected shoot growth, which is evidenced by the low rate of recovered shoots.

scholarly journals Effect of culture media, plant growth regulators and genotypes on growth and developmental stages of oil palm (Elaeis guineensis Jacq.) zygotic embryos

2019 ◽  
Author(s):  
D.S. Sparjanbabu ◽  
P. Naveen Kumar ◽  
M.S.R. Krishna ◽  
D. Ramajayam ◽  
B. Kalyana Babu ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Seed Production ◽  
Growth Regulators ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Culture Media ◽  
Zygotic Embryos ◽  
Elaeis Guineensis Jacq

Oil palm (Elaeis guineensis Jacq.) has become the largest traded vegetable oil in the world due to its innumerable uses and its huge economic importance. Which increases the requirement of quality seedling but the conventional seed production limits the commercial seed production with its long germination period and low germination rate. In this context in vitro germination of zygotic embryo could be a promising alternative. Hence in this study the effect of culturing media, plant growth regulators and genotypes were assessed on in vitro regeneration and plantlet development of oil palm zygotic embryos. Zygotic embryos of four elite genotypes were cultured on MS, N6 and Y3 media, with or without plant growth regulators (NAA, BA and GA3-0.2mg/lt). There was no effect of culture media, plant growth regulators and genotypes on germination of zygotic embryos. Whereas on the growth and development of in vitro plantlets, there was a significant effect of culture media and genotypes. Where culture media N6 performs well and Y3 has shown more abnormality. In case of genotypes, genotypes G1 and G2 shown better performance when compared to genotypes G3 and G4. The effect of plant growth regulators on germination and plant growth was non significant.

LEGITIMACY OF PROGENIES USING SSR MARKERS AS CONTROLLING SYSTEM AND EARLIER SELECTION IN THE OIL PALM NURSERY (Elaeis guineensis JACQ.)

2018 ◽  
Vol 25 (1) ◽  
pp. 21-30
Author(s):  
Rokhana Faizah ◽  
Sri Wening ◽  
Abdul Razak Purba
Keyword(s):  
Dna Fingerprinting ◽  
Ssr Markers ◽  
Oil Palm ◽  
Dna Markers ◽  
Elaeis Guineensis ◽  
Gene Marker ◽  
Controlling System ◽  
Crossing Probability ◽  
Simple Sequence

Information of legitimacy of oil palm progenies is important to guaranty the quality and to control commercial seeds procedures. A true and legitimate cross will produce progeny which has a combination of their parent's allele. The information could be obtained early in the nursery stage through DNA fingerprinting analysis. Simple Sequence Repeats (SSR) is one of DNA markers used for DNA fingerprinting, since the marker system has advantages to acquire information of allele per individual in population and efficiency diverse allele of progeny and their parents. The aim of the research is to obtain legitimacy of 12 progenies analyzing in the oil palm nursery stage. Thirteen SSR markers were used to analyze 12 crossings number of oil palm. The genotypes data by alleles of SSR inferred and quantified using Gene Marker® Software version 2.4.0 Soft Genetics® LLC and analyzed based on Mendel's Law of Segregation. The result showed based on heredity pattern of progeny and their parent's allele that progenies H were indicated genetically derived from their known parents while progenies from A and G indicated as illegitimate crossing. Probability value for legitimacy of progenies of 9 other crosses has 0.031 and 0.5. Legitimacy analysis of progeny using SSR markers could be used to control the quality of crossing material and earlier selection in the oil palm nursery.

scholarly journals Effects of Sucrose and Other Additives on In Vitro Growth and Development of Purple Coneflower (Echinacea purpurea L.)

2014 ◽  
Vol 2014 ◽  
pp. 1-4 ◽  
Author(s):  
Dahanayake Nilanthi ◽  
Yue-Sheng Yang
Keyword(s):  
Plant Growth ◽  
Culture Medium ◽  
Respiratory Infections ◽  
Shoot Multiplication ◽  
Echinacea Purpurea ◽  
Naphthaleneacetic Acid ◽  
Acid Plant ◽  
Purple Coneflower ◽  
Upper Respiratory

Echinacea purpurea (purple coneflower) is being used for the preparation of more than 240 extracts, salves, and tinctures to help cure diseases like rabies, cold, and upper respiratory infections. Hence, efforts were made to develop a culture medium for successful in vitro culturing of cornflower and to regenerate buds and induce roots to enable mass propagation of selected clones. Of the three levels of sucrose tested as a supplement to MS media (Murashige and Skoog’s medium, 1962) 3% showed better rooting of buds and appeared morphologically normal and identical as compared to those grown at higher and lower concentrations (2 and 4%). The additives hydrolyzed lactabumin (0.0, 100, 300, and 900 mgL−1), peptone (0.0, 100, 300, and 900 mgL−1), and yeast (0.0, 100, 300, and 900 mgL−1) to media containing 0.3 mgL−1 BA (6-benzyladenine) and 0.01 mgL−1 NAA (naphthaleneacetic acid-plant growth regulators) has negatively influenced proliferation of shoots. The higher concentrations of the above have delayed the development of plantlets. Shoot multiplication was enhanced by coconut water with 2% being the best among 4 and 8% tested. Shoot organogenesis was not influenced by copper sulphate (0, 1.5, 3, 6, and 12 mgL−1) and silver nitrate (0.0, 0.5, 2.5, and 12.5 mgL−1) supplements and at higher concentrations of the above inhibited plant growth.

scholarly journals Accelerated in vitro propagation of elite oil palm genotypes (Elaeis guineensis Jacq.) by substituting cytokinin with putrescine

2016 ◽  
Vol 15 (50) ◽  
pp. 2767-2775 ◽  
Author(s):  
Roseli Correa Thais ◽  
Yoshimitsu Motoike Sérgio ◽  
Paula de Souza Andrade Ana ◽  
Morra Coser Sara ◽  
Queiroz Vanessa ◽  
...  
Keyword(s):  
Oil Palm ◽  
In Vitro Propagation ◽  
Elaeis Guineensis ◽  
Elaeis Guineensis Jacq

Biochemical markers for pregnancy in the spent culture medium of in vitro produced bovine embryos

2021 ◽  
Author(s):  
Gabriela de Oliveira Fernandes ◽  
Marcella Pecora Milazzotto ◽  
Andrei Antonioni Guedes Fidelis ◽  
Taynan Stonoga Kawamoto ◽  
Ligiane de Oliveira Leme ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Biochemical Markers ◽  
Culture Medium ◽  
Culture Media ◽  
Ionization Mass ◽  
Metabolic Profiles ◽  
Bovine Embryos ◽  
The Media

Abstract The present study aimed to identify biomarkers to assess the quality of in vitro produced (IVP) bovine embryos in the culture media. IVP embryos on Day (D) 5 of development were transferred to individual drops, where they were maintained for the last 48 h of culture. Thereafter, the medium was collected and the embryos were transferred to the recipients. After pregnancy diagnosis, the media were grouped into the pregnant and nonpregnant groups. The metabolic profiles of the media were analyzed via electrospray ionization mass spectrometry, and the concentrations of pyruvate, lactate, and glutamate were assessed using fluorimetry. The spectrometric profile revealed that the media from embryos from the pregnant group presented a higher signal intensity compared to that of the nonpregnant group; the ions 156.13 Da [M + H]+, 444.33 Da [M + H]+, and 305.97 Da [M + H]+ were identified as biomarkers. Spent culture medium from expanded blastocysts (Bx) that established pregnancy had a greater concentration of pyruvate (p = 0.0174) and lesser concentration of lactate (p = 0.042) than spent culture medium from Bx that did not establish pregnancy. Moreover, pyruvate in the culture media of Bx can predict pregnancy with 90.9% sensitivity and 75% specificity. In conclusion, we identified markers in the culture media that helped in assessing the most viable IVP embryos with a greater potential to establish pregnancy.

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