scholarly journals Variability of the 3' terminal of the polymerase gene of Grapevine leafroll-associated virus 3 isolates from Vale do São Francisco, Brazil

2005 ◽  
Vol 30 (2) ◽  
pp. 173-176 ◽  
Author(s):  
Érico de C. Dianese ◽  
Eduardo D. Ramalho ◽  
Daniela M. Cerqueira ◽  
Daniela B. Lopes ◽  
Thor V.M. Fajardo ◽  
...  
Keyword(s):  
North America ◽  
Natural Variation ◽  
Amino Acid Change ◽  
Economic Importance ◽  
Southern Brazil ◽  
Polymerase Gene ◽  
Rt Pcr ◽  
Rna Dependent Rna Polymerase ◽  
Great Economic Importance ◽  
Vitis Spp

Many viral diseases, including leafroll, which is of great economic importance, affect grapevines (Vitis spp.). A complex of eight viruses [Grapevine leafroll-associated virus (GLRaV) -1 to 8] is associated with this disease. The objective of this study was to compare the variability of the 3' terminal region of the polymerase gene of three isolates of GLRaV-3 (Grapevine leafroll-associated virus-3), from Submédio do Vale do Rio São Francisco (Petrolina-PE) with that of other isolates available at the GenBank, including an isolate from North America and another from Southern Brazil. The viral RNA was extracted from three infected ELISA reactive plants and a fragment of 340 bp was amplified, by RT-PCR, using primers that recognize that portion of the polymerase gene found between nucleotides 8267 and 8606. The three isolates from Vale do Rio São Francisco named Pet-1, Pet-2 and Pet-3, showed similarities ranging from 98% and 94%, respectively to the isolates from North America (AF037268) and Southern Brazilian (AF438411). Considering the whole genome, the main variation found was one amino acid change at position 2766 (F2766Y). These preliminary data indicate the existence of a natural variation among GLRaV-3 isolates from grapevines. This could be due to the vegetative propagation and long cycle of the plant, associated with the error-prone nature of RNA-dependent RNA polymerase.

scholarly journals A influência da variação zonal da confluência Brasil-Malvinas na safra da tainha no estado de Santa Catarina entre os anos 2006 a 2016

2020 ◽  
Vol 42 ◽  
pp. e13
Author(s):  
Evandro Oscar Mafra ◽  
Rafael De Oliveira ◽  
Mário Francisco Leal de Quadro
Keyword(s):  
Surface Temperature ◽  
Sea Surface Temperature ◽  
Strong Influence ◽  
Economic Importance ◽  
Sea Surface ◽  
Southern Brazil ◽  
Santa Catarina ◽  
Great Economic Importance ◽  
Fish Harvest ◽  
And Behavior

The Brazil-Malvinas Confluence (CBM) comprises the region located in the southwestern portion of the Atlantic Ocean, with a strong influence on meteorological aspects and oceanographic phenomena, including sea surface temperature (SST). Variations in SST, influence the dynamics and behavior of aquatic species and consequently the fishing productivity of mullet (Mugil liza), a species of great economic importance in the state of Santa Catarina. The present study deals with the analysis of the influence of the CBM zonal variation on the sea surface temperature (SST) in southern Brazil, making a comparison with the data of the fish harvest in the Santa Catarina coast, considering a period of 11 years (2006 to 2016) and in the months of fishing (April to July). From the data analysis it is possible to identify the characteristic behavior of the period and to analyze the influencing factors.

scholarly journals Characterization of a novel recombination event in the Deformed wing bee virus polymerase gene

2018 ◽  
Vol 68 (4) ◽  
pp. 661 ◽  
Author(s):  
G GORAS ◽  
IGA RUETHER ◽  
CH TANANAKI ◽  
S GOUNARI ◽  
V LIOLIOS ◽  
...  
Keyword(s):  
Recombination Event ◽  
Polymerase Gene ◽  
Rt Pcr ◽  
Rna Dependent Rna Polymerase ◽  
Reverse Transcription Pcr ◽  
Plot Analysis ◽  
Recombination Point ◽  
Bee Virus ◽  
Similarity Plot

Honeybee populations are known to be infected by numerous viruses. Reverse transcription-PCR (RT-PCR) of regions of the RNA-dependent RNA polymerase is often used to diagnose the presence in apiaries and also to classify the type of virus detected. In this report, through analysis of the RdRp gene, we describe a novel recombination event in the DWV genome. Similarity plot analysis amplified from hundred positive individuals identified a previously undescribed recombination point in the 5’ region of the polymerase gene. To our knowledge this is the first description of recombination in the DWV polymerase gene and highlights the continuous genetic evolution of these viruses.

scholarly journals The Prevalence of RT-PCR Positivity of SARS-CoV-2 on 10,000 Patients from Three Cities Located on the Eastern of Turkey

2020 ◽  
Author(s):  
Karamese Murat ◽  
Ozgur Didem ◽  
Tarhan Ceyda ◽  
Dik Altintas Susamber ◽  
Caliskan Okan ◽  
...  
Keyword(s):  
Epidemiologic Studies ◽  
Screening Tests ◽  
Gene Fragment ◽  
Polymerase Gene ◽  
Rt Pcr ◽  
Rna Dependent Rna Polymerase ◽  
Final Study ◽  
The World ◽  
Study Population ◽  
Rna Polymerase Gene

ABSTRACTCOVID-19, is caused by SARS-CoV-2, has been started on December/2019 in Wuhan/China and spread all over the world. We analyzed RT-PCR results of 10,000 cases from April-2 to May-30, 2020 in three neighbor cities located on the Eastern of Turkey. The final study population was 7853 cases after excluded screening tests. RT-PCR were performed to detect the SARS-CoV-2-specific RdRp (RNA-dependent-RNA-polymerase) gene fragment. The number of total positive samples out of 7853 were 487; however, the number of non-repeating positive patient was 373 (4.8%). The cough and fever were the most common symptoms in positive cases. The epidemiologic studies should be performed about the prevalence of SARS-CoV-2 infection to better understand the effect of the virus all over the world.

A striate mosaic virus disease of grasses and cereals in Australia, transmitted by the cicadellid Nesoclutha obscura

1963 ◽  
Vol 14 (2) ◽  
pp. 143 ◽  
Author(s):  
NE Grylls
Keyword(s):  
North America ◽  
Host Range ◽  
Mosaic Virus ◽  
Virus Disease ◽  
New South ◽  
Economic Importance ◽  
Perennial Grasses ◽  
Cereal Aphids ◽  
Great Economic Importance ◽  
South Wales

A virus disease of perennial grasses and small grain cereals, not previously studied in Australia, was found in northern New South Wales and Queensland. The disease does not appear at present to be of great economic importance, but the virus has many properties in common with wheat striate mosaic virus of North America. The vector is the cicadellid leafhopper Nesoclutha obscura Evans. Ten other cicadellids, two delphacids, and two cereal aphids did not transmit the disease. Evidence is presented that, unlike the majority of leafhopper-borne viruses, the virus causing the disease inhabits the mesophyll. Some studies of the virus-vector relationships, the host range, and symptomatology, and comparisons of these with those of other grass and cereal viruses are recorded.

Schwingungsüberlagertes Axialformen/Superimposed Oscillated Axial Forming

2020 ◽  
Vol 110 (11-12) ◽  
pp. 838-843
Author(s):  
Philipp Müller ◽  
Bernd-Arno Behrens ◽  
Sven Hübner ◽  
Hendrik Vogt ◽  
Daniel Rosenbusch ◽  
...  
Keyword(s):  
Surface Quality ◽  
Economic Importance ◽  
Combined Effect ◽  
Wird Eine ◽  
Forming Force ◽  
Forming Process ◽  
Forming Technology ◽  
Great Economic Importance ◽  
Gear Geometry

Techniken zur Steigerung der Formgebungsgrenzen in der Umformtechnik sind von hoher wirtschaftlicher Bedeutung. In dieser Arbeit wird eine Schwingungsüberlagerung im Krafthauptfluss eines Axialformprozesses zur Ausprägung einer Verzahnungsgeometrie untersucht. Die Auswirkungen der Schwingung auf die erzielbare Ausfüllung der Zahnkavitäten werden analysiert sowie die Parameter Schmierung und Oberflächengüte der Halbzeuge in ihrer kombinierten Wirkung untersucht. Es konnte eine Reduzierung der mittleren Umformkraft sowie eine Erhöhung der Formfüllung festgestellt werden. Techniques for extending the production limits in forming technology are of great economic importance. In this research, a superimposed oscillation in the main force flow of an axial forming process to form an axial gear geometry is investigated. The effects of the superimposed oscillation on the achievable form-filling of the tooth cavities are analyzed and the parameters lubrication and surface quality of the semi-finished products are investigated in their combined effect. A reduction of the averaged forming force as well as an increase of the form-filling could be achieved.

scholarly journals Extensive genetic diversity and host range of rodent-borne coronaviruses

2020 ◽  
Vol 6 (2) ◽  
Author(s):  
Wen Wang ◽  
Xian-Dan Lin ◽  
Hai-Lin Zhang ◽  
Miao-Ruo Wang ◽  
Xiao-Qing Guan ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Rodent Species ◽  
Polymerase Gene ◽  
Rna Dependent Rna Polymerase ◽  
Reading Frame ◽  
Genome Recombination ◽  
Genomic Complexity ◽  
Viral Subtype ◽  
Rna Polymerase Gene ◽  
Eothenomys Miletus

Abstract To better understand the genetic diversity, host associations and evolution of coronaviruses (CoVs) in China we analyzed a total of 696 rodents encompassing 16 different species sampled from Zhejiang and Yunnan provinces. Based on reverse transcriptase PCR-based CoV screening of fecal samples and subsequent sequence analysis of the RNA-dependent RNA polymerase gene, we identified CoVs in diverse rodent species, comprising Apodemus agrarius, Apodemus chevrieri, Apodemus latronum, Bandicota indica, Eothenomys cachinus, Eothenomys miletus, Rattus andamanensis, Rattus norvegicus, and Rattus tanezumi. CoVs were particularly commonplace in A. chevrieri, with a detection rate of 12.44 per cent (24/193). Genetic and phylogenetic analysis revealed the presence of three groups of CoVs carried by a range of rodents that were closely related to the Lucheng Rn rat CoV (LRNV), China Rattus CoV HKU24 (ChRCoV_HKU24), and Longquan Rl rat CoV (LRLV) identified previously. One newly identified A. chevrieri-associated virus closely related to LRNV lacked an NS2 gene. This virus had a similar genetic organization to AcCoV-JC34, recently discovered in the same rodent species in Yunnan, suggesting that it represents a new viral subtype. Notably, additional variants of LRNV were identified that contained putative non-structural (NS)2b genes located downstream of the NS2 gene that were likely derived from the host genome. Recombination events were also identified in the open reading frame (ORF) 1a gene of Lijiang-71. In sum, these data reveal the substantial genetic diversity and genomic complexity of rodent-borne CoVs, and extend our knowledge of these major wildlife virus reservoirs.

scholarly journals Detecção do Grapevine virus A e Grapevine virus B por hibridização "dot-blot" com sondas moleculares não radioativas

2005 ◽  
Vol 30 (5) ◽  
pp. 538-542
Author(s):  
Andreia E Moreira ◽  
José O Gaspar ◽  
Hugo Kuniyuki
Keyword(s):  
Grapevine Virus ◽  
Rt Pcr ◽  
Dot Blot ◽  
Grapevine Virus A ◽  
Grapevine Virus B ◽  
Vitis Spp ◽  
Kober Stem Grooving

O vírus A da videira (Grapevine virus A, GVA) e o vírus B da videira (Grapevirus virus B, GVB) estão associados à acanaladura do lenho de Kober ("Kober stem grooving") e ao fendilhamento cortical da videira ("grapevine corky bark"), respectivamente. Este trabalho descreve o uso de sondas moleculares de cDNA na detecção de isolados do GVA (GVA-SP) e do GVB (GVB-C-SP e GVB-I-SP) em videiras (Vitis spp.) e fumo (Nicotiana occidentalis). As sondas marcadas com digoxigenina foram produzidas por RT-PCR utilizando oligonucleotídeos específicos para os genes da proteína capsidial. Os RNA totais foram extraídos de 45 plantas de diversas variedades de videira e de 13 plantas de fumo inoculadas mecanicamente com o GVB. Os RNA extraídos das plantas infetadas, indexadas biologicamente, hibridizaram com as sondas, não se verificando reação com plantas sadias. Para confirmar os resultados de hibridização, foram também feitos testes de RT-PCR. A utilização de hibridização "dot-blot" com sondas de cDNA mostrou-se eficaz na detecção dos vírus com especificidade e sensibilidade, ressaltando-se que, preferencialmente, folhas maduras e ramos dormentes devem ser utilizados nos testes diagnósticos para o GVB e GVA, respectivamente.

scholarly journals First Report of Tomato spotted wilt virus in Blackberry Lily in North America

Plant Disease ◽  
2003 ◽  
Vol 87 (1) ◽  
pp. 102-102 ◽  
Author(s):  
S. Adkins ◽  
L. Breman ◽  
C. A. Baker ◽  
S. Wilson
Keyword(s):  
North America ◽  
Tomato Spotted Wilt Virus ◽  
Amino Acid Sequences ◽  
South Florida ◽  
N Gene ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
First Report ◽  
Tomato Spotted Wilt ◽  
Wilt Virus

Blackberry lily (Belamcanda chinensis (L.) DC.) is an herbaceous perennial in the Iridaceae characterized by purple-spotted orange flowers followed by persistent clusters of black fruit. In July 2002, virus-like symptoms including chlorotic ringspots and ring patterns were observed on blackberry lily leaves on 2 of 10 plants in a south Florida ornamental demonstration garden. Inclusion body morphology suggested the presence of a Tospovirus. Tomato spotted wilt virus (TSWV) was specifically identified by serological testing using enzyme-linked immunosorbent assay (Agdia, Elkhart, IN). Sequence analysis of a nucleocapsid (N) protein gene fragment amplified by reverse transcription-polymerase chain reaction (RT-PCR) with primers TSWV723 and TSWV722 (1) from total RNA confirmed the diagnosis. Nucleotide and deduced amino acid sequences of a 579 base pair region of the RT-PCR product were 95 to 99% and 95 to 100% identical, respectively, to TSWV N-gene sequences in GenBank. Since these 2-year-old plants were grown on-site from seed, they were likely inoculated by thrips from a nearby source. Together with a previous observation of TSWV in north Florida nursery stock (L. Breman, unpublished), this represents, to our knowledge, the first report of TSWV infection of blackberry lily in North America although TSWV was observed in plants of this species in Japan 25 years ago (2). References: (1) S. Adkins, and E. N. Rosskopf. Plant Dis. 86:1310, 2002. (2) T. Yamamoto and K.-I. Ohata. Bull. Shikoku Agric. Exp. Stn. 30:39, 1977.

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