The effect of the essential oils from five different Lippia species on the viability of tumor cell lines

EucalyptusL. is traditionally used for many medicinal purposes. In particular, someEucalyptusspecies have currently shown cytotoxic properties. Local Brazilian communities have used leaves ofE. benthamiias a herbal remedy for various diseases, including cancer. Considering the lack of available data for supporting this cytotoxic effect, the goal of this paper was to study thein vitrocytotoxic potential of the essential oils from young and adult leaves ofE. benthamiiand some related terpenes (α-pinene, terpinen-4-ol, andγ-terpinene) on Jurkat, J774A.1 and HeLa cells lines. Regarding the cytotoxic activity based on MTT assay, the essential oils showed improved results thanα-pinene andγ-terpinene, particularly for Jurkat and HeLa cell lines. Terpinen-4-ol revealed a cytotoxic effect against Jurkat cells similar to that observed for volatile oils. The results of LDH activity indicated that cytotoxic activity of samples against Jurkat cells probably involved cell death by apoptosis. The decrease of cell DNA content was demonstrated due to inhibition of Jurkat cells proliferation by samples as a result of cytotoxicity. In general, the essential oils from young and adult leaves ofE. benthamiipresented cytotoxicity against the investigated tumor cell lines which confirms their antitumor potential.

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Cytotoxic potential of essential oils of Eucalyptus benthamii and its related terpenes on tumor cell lines

Planta Medica ◽

10.1055/s-0035-1556209 ◽

2015 ◽

Vol 81 (11) ◽

Author(s):

PV Farago ◽

PM Döll-Boscardin ◽

CC Kanunfre ◽

JM Budel

Keyword(s):

Tumor Cell ◽

Essential Oils ◽

Cell Lines ◽

Tumor Cell Lines ◽

Cytotoxic Potential

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Antimicrobial Activity of Essential Oils againstStreptococcus mutansand their Antiproliferative Effects

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2012/751435 ◽

2012 ◽

Vol 2012 ◽

pp. 1-12 ◽

Cited By ~ 35

Author(s):

Lívia Câmara de Carvalho Galvão ◽

Vivian Fernandes Furletti ◽

Salete Meyre Fernandes Bersan ◽

Marcos Guilherme da Cunha ◽

Ana Lúcia Tasca Góis Ruiz ◽

...

Keyword(s):

Antimicrobial Activity ◽

Tumor Cell ◽

Essential Oils ◽

Cell Lines ◽

Antiproliferative Activity ◽

Human Tumor ◽

Gas Chromatography Mass Spectrometry ◽

Tumor Cell Lines ◽

Human Tumor Cell ◽

Human Tumor Cell Lines

This study aimed to evaluate the activity of essential oils (EOs) againstStreptococcus mutansbiofilm by chemically characterizing their fractions responsible for biological and antiproliferative activity. Twenty EO were obtained by hydrodistillation and submitted to the antimicrobial assay (minimum inhibitory (MIC) and bactericidal (MBC) concentrations) againstS. mutansUA159. Thin-layer chromatography and gas chromatography/mass spectrometry were used for phytochemical analyses. EOs were selected according to predetermined criteria and fractionated using dry column; the resulting fractions were assessed by MIC and MBC, selected as active fractions, and evaluated againstS. mutansbiofilm. Biofilms formed were examined using scanning electron microscopy. Selected EOs and their selected active fractions were evaluated for their antiproliferative activity against keratinocytes and seven human tumor cell lines. MIC and MBC values obtained for EO and their active fractions showed strong antimicrobial activity. Chemical analyses mainly showed the presence of terpenes. The selected active fractions inhibitedS. mutansbiofilm formation (P<0.05) did not affect glycolytic pH drop and were inactive against keratinocytes, normal cell line. In conclusion, EO showed activity at low concentrations, and their selected active fractions were also effective against biofilm formed byS. mutansand human tumor cell lines.

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Chemical Analysis of Essential Oils from Ocotea gomezii W.C. Burger and Ocotea morae Gómez-Laur. (Lauraceae) Collected at "Reserva Biológica Alberto M. Brenes" in Costa Rica and their Cytotoxic Activity on Tumor Cell Lines

Journal of the Brazilian Chemical Society ◽

10.1590/s0103-50532011000400018 ◽

2011 ◽

Vol 22 (4) ◽

pp. 741-745 ◽

Cited By ~ 6

Author(s):

Carlos Chaverri ◽

Cecilia Díaz ◽

José F. Cicció

Keyword(s):

Costa Rica ◽

Chemical Analysis ◽

Tumor Cell ◽

Essential Oils ◽

Cytotoxic Activity ◽

Cell Lines ◽

Tumor Cell Lines

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β-Caryophyllene, a Compound Isolated from the Biblical Balm of Gilead (Commiphora gileadensis), Is a Selective Apoptosis Inducer for Tumor Cell Lines

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2012/872394 ◽

2012 ◽

Vol 2012 ◽

pp. 1-8 ◽

Cited By ~ 46

Author(s):

Eitan Amiel ◽

Rivka Ofir ◽

Nativ Dudai ◽

Elaine Soloway ◽

Tatiana Rabinsky ◽

...

Keyword(s):

Tumor Cell ◽

Essential Oils ◽

Cell Lines ◽

Tumor Cell Lines ◽

Dna Ladder ◽

Normal Cells ◽

Cytotoxic Compound ◽

Wide Range ◽

Food And Beverage ◽

Apoptosis Inducer

The biblical balm of Gilead (Commiphora gileadensis) was investigated in this study for anticancerous activity against tumor cell lines. The results obtained from ethanol-based extracts and from essential oils indicated thatβ-caryophyllene (trans-(1R,9S)-8-methylene-4,11,11-trimethylbicyclo[7.2.0]undec-4-ene) is a key component in essential oils extracted from the balm of Gilead.β-Caryophyllene can be found in spice blends, citrus flavors, soaps, detergents, creams, and lotions, as well as in a variety of food and beverage products, and it is known for its anti-inflammatory, local anaesthetic, and antifungal properties. It is also a potent cytotoxic compound over a wide range of cell lines. In the current paper, we found thatCommiphora gileadensisstem extracts and essential oil have an antiproliferative proapoptotic effect against tumor cells and not against normal cells.β-caryophyllene caused a potent induction of apoptosis accompanied by DNA ladder and caspase-3 catalytic activity in tumor cell lines. In summary, we showed thatC. gileadensisstems contain an apoptosis inducer that acts, in a selective manner, against tumor cell lines and not against normal cells.

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Expression of HIF-1a and VEGF in human pituitary tumors and rodent pituitary tumor cell lines under hypoxia-mimicking condition

Experimental and Clinical Endocrinology & Diabetes ◽

10.1055/s-0028-1096352 ◽

2008 ◽

Vol 116 (09) ◽

Author(s):

B Shan ◽

C Onofri ◽

M Theodoropoulou ◽

E Arzt ◽

GK Stalla ◽

...

Keyword(s):

Tumor Cell ◽

Cell Lines ◽

Pituitary Tumor ◽

Pituitary Tumors ◽

Tumor Cell Lines ◽

Human Pituitary ◽

Pituitary Tumor Cell

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Interaction of Human Tumor Cells with Human Platelets and the Coagulation System

Thrombosis and Haemostasis ◽

10.1055/s-0038-1665296 ◽

1983 ◽

Vol 50 (03) ◽

pp. 726-730 ◽

Cited By ~ 11

Author(s):

Hamid Al-Mondhiry ◽

Virginia McGarvey ◽

Kim Leitzel

Keyword(s):

Tumor Cell ◽

Tumor Cells ◽

Cell Lines ◽

Human Tumor ◽

Human Platelets ◽

Factor Vii ◽

Coagulation System ◽

Breast Adenocarcinoma ◽

Activate Factor ◽

Tumor Cell Lines

SummaryThis paper reports studies on the interaction between human platelets, the plasma coagulation system, and two human tumor cell lines grown in tissue culture: Melanoma and breast adenocarcinoma. The interaction was monitored through the use of 125I- labelled fibrinogen, which measures both thrombin activity generated by cell-plasma interaction and fibrin/fibrinogen binding to platelets and tumor cells. Each tumor cell line activates both the platelets and the coagulation system simultaneously resulting in the generation of thrombin or thrombin-like activity. The melanoma cells activate the coagulation system through “the extrinsic pathway” with a tissue factor-like effect on factor VII, but the breast tumor seems to activate factor X directly. Both tumor cell lines activate platelets to “make available” a platelet- derived procoagulant material necessary for the conversion of prothrombin to thrombin. The tumor-derived procoagulant activity and the platelet aggregating potential of cells do not seem to be inter-related, and they are not specific to malignant cells.

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