scholarly journals STUDI ANATOMIS DAN HISTOLOGIS PANKREAS BIAWAK AIR (Varanus salvator) (Anatomical and Histological Study of the Pancreas of Water Monitor Lizard (Varanus salvator))

2016 ◽  
Vol 10 (2) ◽  
pp. 153-156 ◽  
Author(s):  
Hamny Hamny ◽  
Muhammad Iqbal ◽  
Sri Wahyuni ◽  
Mustafa Sabri ◽  
M. Jalaluddin ◽  
...  
Keyword(s):  
Histological Study ◽  
Histological Structure ◽  
Ventral Lobe ◽  
Nacl Solution ◽  
Dorsal Lobe ◽  
Yellow Color ◽  
Monitor Lizard ◽  
Oval Form ◽  
Varanus Salvator ◽  
Hematoxylin Eosin

The aim of this research was to determine the anatomy and histology of pancreas of water monitor lizard (Varanus salvator). Two pancreas were used in this research (a male and a female). Water monitor lizard was euthanized using chloroform and subsequently necropsied to collect the pancreas. The pancreas was washed in 0.9 % NaCl solution. Anatomical observations were carried out including location, shape, and color of pancreas, also the length and weight measurements. Then, pancreas was fixed with 4% paraformaldehyde for 7 days to proceed to histological preparation and hematoxylin eosin (HE) staining. The results of the anatomical observations showed that pancreas of water monitor lizard have two lobes, dorsal lobe (splenic lobe) and ventral lobe (duodenal lobe). Dorsal lobe which was in oval form adhered with the spleen, and ventral lobe was located in the cranial of intestines with the form of long spherical. Both of pancreas lobes were white-yellow color with the length of 1.45±0.64 cm (dorsal lobe) and 7.00±0.42 cm (ventral lobe). Weight of dorsal lobe was 0.15±0.01 g and ventral lobe was 6.35±2.30 g. The histological observations found the acinar cells, ducts, and blood vessels in both of pancreas lobes. Additionally, the similar structure of Langerhans islet only found at the ventral lobe of pancreas. In conclusion, pancreas of water monitor lizard consists of two lobes which located differently. Ventral lobe is larger than the dorsal lobe. Both lobes have similar histological structure except the Langerhans islets are only appeared in the ventral lobe.

Studi Histokimia Usus Biawak Air (Varanus salvator bivittatus) (Reptil: Varanidae)

2019 ◽  
Author(s):  
Mahfud Mahfud ◽  
Ernawati
Keyword(s):  
Tissue Sample ◽  
Histological Section ◽  
Tissue Perfusion ◽  
Intestinal Wall ◽  
Biological Information ◽  
Suitable Habitat ◽  
Monitor Lizard ◽  
Varanus Salvator ◽  
Hematoxylin Eosin ◽  
Paraffin Method

Biological information, behaviour and suitable habitat of water monitor was very less in order to support its maintenance management and breeding efforts. One of important information is the information of digestive tract, particularly the information about the structure of intestine tissue of water monitor. Sample in this research was intestine organ of water monitor. The animal was anesthetized, exanguinated, and fixed in paraformaldehyde 4% by tissue perfusion method. The intestine tissue sample for histological section with paraffin method was cutted with 3-4 μm thick and coloured with hematoxylin eosin (HE). Observation were performed to the structure of intestine histology. The results was analysed descriptively and presented in figures. Monitor lizard intestine consist of small intestine and large intestine. The small intestinal wall was observed similar to jejunum and ileum. The large intestinal wall was composed of transitional ephytelia and connective tissue. However, the ephytelial layer in this tissue was composed of transitional ephytelia that similar to vesica urinaria and there are no villi.

scholarly journals Micromorphological Structure of Primary Reproductive Organ of Male Water Monitor Lizard (Varanus salvator bivittatus)

2016 ◽  
Vol 10 (1) ◽  
Author(s):  
Mahfud M ◽  
Adi Winarto ◽  
Chairun Nisa
Keyword(s):  
Reproductive Organ ◽  
Columnar Epithelium ◽  
Histological Structure ◽  
Ductus Deferens ◽  
Tissue Samples ◽  
Histological Techniques ◽  
Circular Smooth Muscle ◽  
Monitor Lizard ◽  
Mucosal Layer ◽  
Varanus Salvator

The study aims to determine the histological structure of the testes, ductus epididymidis and ductus deferens of Varanus salvator bivittatus. The tissue samples were obtained by the histological techniques preparation with thickness section is 3-4 µm then stained using hematoxylineosin(HE)andMasson'sTrichrome(MT).Theresultshowedthattestesarecoveredbytunicavaginalisandtunicaalbuginea(tunicafibrousandtunicavasculosa). Tubulus seminiferous as a parenchyma are found in lobules of testes which are formed by septum as extension of tunicaalbuginea. Tubulus seminiferous is composed by layers of germinal epithelium cells consist of spermatogenia, Sertoli cell, spermatocyte andspermatid which is developed further to be spermatozoa in the lumen of tubulus. Among the tubulus seminiferous, there are groups of epithelioidcells called Leydig cells. The end of tubulus seminiferous formed ductus epididymidis which then ended up and known as ductus deferens.Epididymis is covered by dense connective tissue. Ductus epididymidis is divided into three segments: cranial, medial and caudal. It is lined bypseudostratified columnar epithelium which is varied in its thickness, amount and diameter of lumen. Lumen of the ductus deferens was lined bypseudostratified ciliated columnar epithelium and thin muscularis mucosal layer and thick circular smooth muscle were covered externally.Key words: Varanus salvator bivittatus, testes, ductus epididymidis, ductus deferens, micromorphology 

scholarly journals Histological Structure of Varanus Salvator Intestine

2020 ◽  
Vol 3 ◽  
pp. 121-124
Author(s):  
Risti Widyaningsih
Keyword(s):  
Blood Vessel ◽  
Muscle Layer ◽  
Common Species ◽  
Histological Structure ◽  
Histological Observation ◽  
Monitor Lizard ◽  
The Family ◽  
Varanus Salvator ◽  
Paraffin Method ◽  
Muscularis Externa

Varanus salvator is a common species in Indonesia. This animal is known as the water lizard, who is a member of the family Varanidae or known as the monitor lizard. This research aims to find out histological structure of Varanus salvator intestine. Histological observation was carried out by making histological preparations using the paraffin method. The results of this study were analyzed in a comparative descriptive manner through observation in a microscope and transverse photo histology preparations using a camera. The results of this study indicate that the intestinal histological structure of Varanus salvator consists of Serosa (Sr), Muscle layer (Ml), Muscularis externa (Me), Submucosa (Sm), Mucosa (M), Blood Vessel (Bv) dan Lamina Propria (Lp).

scholarly journals A Comparative Histological Study of Skin in Clarias gariepinus and Oreochromis niloticus

2021 ◽  
Vol 32 (2) ◽  
pp. 90
Author(s):  
Shafia Khairani ◽  
Faisal Fikri ◽  
Agus Purnomo ◽  
Muhammad Thohawi Elziyad Purnama
Keyword(s):  
Oreochromis Niloticus ◽  
Histological Study ◽  
Clarias Gariepinus ◽  
Histological Structure ◽  
Club Cells ◽  
Basement Layer ◽  
Hematoxylin Eosin ◽  
Dermis Layer

This study aimed to evaluate the comparative of histological figures in Clarias gariepinus and Oreochromis niloticus skin. A total of 18 samples were observed and collected the skin preservation. The hematoxylin-eosin staining performed to determine the histological structure. Results showed that there were different structures in the epidermis and dermis layer between C. gariepinus and O. niloticus skin. Club cells showed dominated in the epidermis layer of C. gariepinus. The pigmented cell showed clearly in the basement layer of C. gariepinus compared to O. niloticus. Stratum compactum in O. niloticus also showed adequate compared to C. gariepinus.

scholarly journals Histological Study of Mudskipper (Periophthalmus gracilis) Gills

2019 ◽  
Vol 2 ◽  
pp. 177-179
Author(s):  
Hikmah Supriyati ◽  
Nurul Safitri Apriliani ◽  
Muhammad Ja’far Luthfi
Keyword(s):  
Histological Study ◽  
Gill Filament ◽  
Histological Structure ◽  
Respiratory Organ ◽  
Secondary Lamellae ◽  
Long Time ◽  
Qualitative Descriptive ◽  
Gill Structure ◽  
And Behavior ◽  
Hematoxylin Eosin

Mudskipper belongs to the Gobiidae family which has respiratory adaptation to fits their habitat. Mudskipper has a different gill structure or modification of gills, this different structure allows the mudskipper to survive for a long time outside water. This study aimed to determine the histology of gills and find out whether there is a modification of gills in the mudskipper respiratory organs (Periophthalmus gracilis). Histological preparations were done using paraffin method, stained with Hematoxylin-Eosin. Data analysis was carried out in a qualitative descriptive. The results showed that there is no additional respiratory organ in the mudskipper respiration, whereas the gills have some modifications. The histological structure of mudskipper gills consists of gill arches, arteries, gill filament, primary lamellae, and secondary lamellae. The gills of mudskipper have a different structure from the general fish, which has a thick secondary lamellae with a low amount of density, the shape of the filament are short and bent. This gill structure is a form of adaptation to habitat and behavior to live outside the water in a relatively long time.

scholarly journals Histological Structure of the Varanus Salvator Kidney

2020 ◽  
Vol 3 ◽  
pp. 117-119
Author(s):  
Ida Khoirunnisa’
Keyword(s):  
Proximal Convoluted Tubule ◽  
Distal Convoluted Tubule ◽  
Histological Structure ◽  
Physics Laboratory ◽  
Monitor Lizard ◽  
Varanus Salvator ◽  
Monitor Lizards ◽  
Bowman's Capsule ◽  
Paraffin Method

Varanus salvator is known as the monitor lizard. This animal is a subspecies of endemic Indonesian monitor lizards which is only found in Java, Bali and Nusa Tenggara (except Timor Island). This study aims to determine the histological structure of the monitor lizard (Varanus salvator). The research was carried out for two months starting from September 2019 to October 2019 at the Animal Physics Laboratory and Zoology of UIN Sunan Kalijaga. The method used is the paraffin method. The results of the study were analyzed in a comparative descriptive manner by observation through a microscope and then taken using a camera. Based on the results of studies lizards (Varanus salvator) have distal convoluted tubule, proximal convoluted tubule, glomerulus, medullary ray and bowman’s capsule.

Anticancer Properties of Asian Water Monitor Lizard (Varanus salvator), Python (Malayopython reticulatus) and Tortoise (Cuora kamaroma amboinensis)

2020 ◽  
Vol 20 (13) ◽  
pp. 1558-1570
Author(s):  
Shareni Jeyamogan ◽  
Naveed A. Khan ◽  
Kuppusamy Sagathevan ◽  
Ruqaiyyah Siddiqui
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Amino Acid ◽  
Anticancer Agents ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Adenocarcinoma Cells ◽  
Monitor Lizard ◽  
Varanus Salvator ◽  
Asian Water

Background: Cancer contributes to significant morbidity and mortality despite advances in treatment and supportive care. There is a need for the identification of effective anticancer agents. Reptiles such as tortoise, python, and water monitor lizards are exposed to heavy metals, tolerate high levels of radiation, feed on rotten/germ-infested feed, thrive in unsanitary habitat and yet have prolonged lifespans. Such species are rarely reported to develop cancer, suggesting the presence of anticancer molecules/mechanisms. Methods: Here, we tested effects from sera of Asian water monitor lizard (Varanus salvator), python (Malayopython reticulatus) and tortoise (Cuora kamaroma amboinensis) against cancer cells. Sera were collected and cytotoxicity assays were performed using prostate cancer cells (PC3), Henrietta Lacks cervical adenocarcinoma cells (HeLa) and human breast adenocarcinoma cells (MCF7), as well as human keratinized skin cells (Hacat), by measuring lactate dehydrogenase release as an indicator for cell death. Growth inhibition assays were performed to determine the effects on cancer cell proliferation. Liquid chromatography mass spectrometry was performed for molecular identification. Results: The findings revealed that reptilian sera, but not bovine serum, abolished viability of Hela, PC3 and MCF7 cells. Samples were subjected to liquid chromatography mass spectrometry, which detected 57 molecules from V. salvator, 81 molecules from Malayopython reticulatus and 33 molecules from C. kamaroma amboinensis and putatively identified 9 molecules from V. salvator, 20 molecules from Malayopython reticulatus and 9 molecules from C. kamaroma amboinensis when matched against METLIN database. Based on peptide amino acid composition, binary profile, dipeptide composition and pseudo-amino acid composition, 123 potential Anticancer Peptides (ACPs) were identified from 883 peptides from V. salvator, 306 potential ACPs from 1074 peptides from Malayopython reticulatus and 235 potential ACPs from 885 peptides from C. kamaroma amboinensis. Conclusion: To our knowledge, for the first time, we reported comprehensive analyses of selected reptiles’ sera using liquid chromatography mass spectrometry, leading to the identification of potentially novel anticancer agents. We hope that the discovery of molecules from these animals will pave the way for the rational development of new anticancer agents.

scholarly journals Tracheotomy in growing rats: histological aspects

2003 ◽  
Vol 18 (3) ◽  
pp. 189-195 ◽  
Author(s):  
Mônica Cecília Bochetti Manna ◽  
Edna Frasson de Souza Montero ◽  
Maria Antonieta Longo Galvão da Silva ◽  
Yara Juliano
Keyword(s):  
Inflammatory Infiltrate ◽  
Sham Group ◽  
Histological Study ◽  
Reference Pattern ◽  
Microsurgical Technique ◽  
Growing Rats ◽  
Global Comparison ◽  
Different Types ◽  
Hematoxylin Eosin ◽  
Epidermoid Metaplasia

PURPOSE: To compare morphologically three different types of tracheotomy in growing rats, applying microsurgical technique. METHODS: EPM-1 Wistar growing rats (n=57) weighing 88gm and aged 35 days were randomized in four groups, according tracheotomy incision type (longitudinal, transverse and tracheal segment excision), and sham group. Following intramuscular anesthesia with ketamine and xylazine, the trachea was exposed and incised, according to the group, and a hand-made endotracheal cannula was inserted into the organ, under sterile conditions. This cannula was removed after 7 days, and animals have been sacrificed 30 days later. Tracheas samples were submitted to histological study, stained by hematoxylin-eosin and Masson trichrome, evaluating fibrosis, inflammatory infiltrate and epidermoid metaplasia. RESULTS: There was more frequency of inflammatory infiltrate at the tracheal epithelium in the tracheal segment excision group (87%) compared to the longitudinal (40%) and transverse (36%) incision groups (p=0.009). Evaluating epidermoid metaplasia, tracheal segment excision and the longitudinal groups presented 33% and 40%, respectively, compared to 0% of the transverse group (p=0.03). Concerning to fibrosis, in a global comparison (p=0.1) among the three groups there was no difference, however, compared to the longitudinal group the transverse group showed lower level of fibrosis (p=0.04). Sham group did not present any relevant morphologic alterations and it was used as reference pattern. CONCLUSION: Taken together, our data show that tracheal segment excision promotes more epithelium aggression and transverse tracheal incision shows less morphologic alterations.

scholarly journals Histological analysis of the tongue, esophagus and crop of Southern Carcará (Polyborus plancus)

2021 ◽  
Vol 10 (2) ◽  
pp. e21410212439
Author(s):  
Vanessa Sobue Franzo ◽  
Leiny Paula de Oliveira ◽  
Ana Carolina Pôssas Viana ◽  
Adriano Tony Ramos ◽  
Jéssica Fernanda Bertolino ◽  
...  
Keyword(s):  
Digestive System ◽  
Histological Analysis ◽  
Squamous Epithelium ◽  
Eating Habits ◽  
Histological Study ◽  
Pekin Ducks ◽  
The Family ◽  
Body Weights ◽  
Stratified Squamous Epithelium ◽  
Hematoxylin Eosin

The Southern Caracara is a bird of prey, of the family Falconidae, inhabits all the Brazilian territory, they are omnivorous birds adapted for hunting. The digestive system of the predators presents differences in their morphology due to their eating habits. Knowing the importance of the gastrointestinal system, this study aims to carry out a histological study of the tongue, esophagus and the crop of Polyborus plancus. For this purpose, eight avian of both sexes, with different body weights and ages were used, they were euthanized and the organs were collected, fixed, dehydrated and stained with hematoxylin-eosin. It was noted that the tongue has stratified squamous epithelium with mucous glands and with different degrees of keratinization. The esophagus and crop have four well developed muscle layers and mucous glands. Additionally, in the crop there is presence of an adventitious layer that consists of loose connective tissue. We conclude that the tongue, the esophagus and the crop of the Southern Caracara have similar histological organization of several birds like the Pekin Ducks, ostriches, domestic chicken and partridges.

scholarly journals Morphological and Histological Study of an ‘Iliac Venous Ladder’ Associated with Very Short Common Iliac Arteries

2019 ◽  
Vol 36 (04) ◽  
pp. 255-260
Author(s):  
Satheesha B. Nayak ◽  
Sudarshan Surendran ◽  
Venu Madhav Nelluri ◽  
Prakashchandra Shetty
Keyword(s):  
Developmental Process ◽  
Present Report ◽  
Histological Study ◽  
Vena Cava ◽  
Iliac Vein ◽  
Histological Structure ◽  
Common Iliac Vein ◽  
Structural Variations ◽  
Surgical Interventions ◽  
The Common

Abstract Introduction Communications between iliac veins in the pelvis are reported to be rare occurrences, which are mostly due to developmental abnormalities. The common iliac vein is formed by the joining of the internal and external iliac veins. Here, we present a detailed morphological and histological study of a rare communication found between the internal and external iliac veins, which would prove to be of substantial value to the knowledge of vessels in the pelvis, both for clinicians and surgeons. Materials and Methods In the present study, we came across a rare communication in the form of a ladder, between the left internal and external iliac veins in a 70-year-old male cadaver. There were two communications (named upper and lower communications) between the external and internal iliac veins, before they joined to form the common iliac vein. On naked eye observation, the lumen of the right common iliac vein appeared to be wider than on the left side. The lengths of these communications and the distance between each of their attachments have been measured and tabulated. The walls of these veins, their microscopic sections and their communication involved in the case were taken. The possible causes for these variations were congenital, owing to the complicated nature of the developmental process involved in the formation of the inferior vena cava and the venous system of the lower limb. Results The structure of all the veins involved in this case and the communications were seen to be normal. The thickness of these walls were measured and tabulated for uniformity around the circumference of the wall of these veins. All the measurements from the structural variations and from the histological observations are tabulated in our results. Conclusion The complicated development sequence of these veins could have possibly led to the persistence of these communications. Such a developmental variation does not seem to pose any threat until unless encountered under clinical or surgical interventions, as the histological structure of the walls of the communications as well as the major channels connected appeared to be normal and well-developed. The detailed morphological and histological features of these structures involved in the variation along with the possible complications have been presented in the present report. Knowledge of these variations and complications due to injury plays a key role in a clinical setup.

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