Histological changes in free jejunal grafts used in pharyngeal reconstruction

1991 ◽  
Vol 105 (7) ◽  
pp. 556-557 ◽  
Author(s):  
J. A. J. Deans ◽  
J. Hill ◽  
M. Bennett
Keyword(s):  
Inflammatory Infiltrate ◽  
Histological Study ◽  
Histological Changes ◽  
Free Grafts ◽  
Pharyngeal Reconstruction

AbstractA histological study was performed of biopsies taken from jejunal free grafts used in pharyngeal reconstruction. The main findings were a decreased crypt/villi ratio and a mild chronic inflammatory infiltrate. There was no evidence of metaplastic or dysplastic transformation.

Cytological and Histological Study of Adult and Neonate Epidermis in Thick and Thin Skin of Various Anatomical Sites

2018 ◽  
Vol 9 (2) ◽  
Author(s):  
Hydar Muhsin Khalfa ◽  
Adnan Albideri ◽  
Haider Salih Jaffat
Keyword(s):  
Histological Study ◽  
Single Layer ◽  
Sweat Glands ◽  
Anatomical Site ◽  
Basal Cells ◽  
Histological Changes ◽  
Mature Adult ◽  
Skin Development ◽  
Stage Of Development ◽  
Thin Skin

The integumentary system covers the surface of the embryo (skin) and its specialized skin structures including hair, nails, sweat glands, mammary glands and teeth. During fetal skin development, the epidermis changes from a single layer of ectodermal cells at 7–8 days of gestation into a more apparent stratified, keratinized epithelium at 22–24 weeks. The aim of the study is to identify the histological and cytological changes that take place during neonatal and adult epidermis development. Human neonatal and adult samples were obtained from fully informed, consenting parent or releatives from Al-hilla mortary / Iraq. Neonatal samples were obtained from neonates after sudden deaths from maternity wards. Anatomical Sites included abdomen, forehead, back, shoulder and feet sole. A totoal of 15 neonates and 10 mature adults were used for this study. Fresh tissues were sectioned using a freezing cryostat. Tissues were sectioned at 5µm in -24°C and collected on microscopic slides. Slides were allowed to air dry for 30 min prior to hematoxyline and eosin staining. Tissues were also photographed using scanning electron microscopy SEM. Cytological measurements were taken using image j software and data was analysed using graph prism. Various cytological and histological changes takes place during neonatal and adult and epidermis development. Our study shows the stages of fair follicule formation as well as number of nucleated layers present at each stage of development and at different anatomical sites. Major histological changes takes places during the transition frm a neonate to a mature adult including the number of basal cells and epidermal thickness depending on the anatomical site.

scholarly journals Tracheotomy in growing rats: histological aspects

2003 ◽  
Vol 18 (3) ◽  
pp. 189-195 ◽  
Author(s):  
Mônica Cecília Bochetti Manna ◽  
Edna Frasson de Souza Montero ◽  
Maria Antonieta Longo Galvão da Silva ◽  
Yara Juliano
Keyword(s):  
Inflammatory Infiltrate ◽  
Sham Group ◽  
Histological Study ◽  
Reference Pattern ◽  
Microsurgical Technique ◽  
Growing Rats ◽  
Global Comparison ◽  
Different Types ◽  
Hematoxylin Eosin ◽  
Epidermoid Metaplasia

PURPOSE: To compare morphologically three different types of tracheotomy in growing rats, applying microsurgical technique. METHODS: EPM-1 Wistar growing rats (n=57) weighing 88gm and aged 35 days were randomized in four groups, according tracheotomy incision type (longitudinal, transverse and tracheal segment excision), and sham group. Following intramuscular anesthesia with ketamine and xylazine, the trachea was exposed and incised, according to the group, and a hand-made endotracheal cannula was inserted into the organ, under sterile conditions. This cannula was removed after 7 days, and animals have been sacrificed 30 days later. Tracheas samples were submitted to histological study, stained by hematoxylin-eosin and Masson trichrome, evaluating fibrosis, inflammatory infiltrate and epidermoid metaplasia. RESULTS: There was more frequency of inflammatory infiltrate at the tracheal epithelium in the tracheal segment excision group (87%) compared to the longitudinal (40%) and transverse (36%) incision groups (p=0.009). Evaluating epidermoid metaplasia, tracheal segment excision and the longitudinal groups presented 33% and 40%, respectively, compared to 0% of the transverse group (p=0.03). Concerning to fibrosis, in a global comparison (p=0.1) among the three groups there was no difference, however, compared to the longitudinal group the transverse group showed lower level of fibrosis (p=0.04). Sham group did not present any relevant morphologic alterations and it was used as reference pattern. CONCLUSION: Taken together, our data show that tracheal segment excision promotes more epithelium aggression and transverse tracheal incision shows less morphologic alterations.

scholarly journals A study of histological changes of human placenta in rural population of eastern India

2018 ◽  
Vol 7 (8) ◽  
pp. 3280
Author(s):  
Mrinal Kanti Karmakar ◽  
Sambit Kar ◽  
S. M. Kumar ◽  
Subir Kumar Chattopadhyay ◽  
L. K. Vaid ◽  
...  
Keyword(s):  
Umbilical Cord ◽  
Cellular Proliferation ◽  
Histological Study ◽  
Normal Growth ◽  
Normal Pregnancy ◽  
Eastern India ◽  
Histological Changes ◽  
Endothelial Proliferation ◽  
Umbilical Cord Insertion ◽  
Normal Placenta

Background: Placenta is essential for maintenance of pregnancy and for promoting normal growth and development of fetus. It forms the morphological record of anatomical condition, intrauterine events and intrapartum events of gestation. Present study has been undertaken to record the data on the morphology and histology of placenta from mothers with hypertension and diabetes.Methods: This study showed several significant morphological and histological differences in the placenta of the mother with GDM and hypertensive placenta. The histological study of the placenta was done under microscope and number of syncytial knots, cytotrophoblastic cellular proliferation, fibrinoid necrosis, endothelial proliferation, calcified and hyalinised villous spots were noted per low power field in the diabetics and hypertensive group in comparison to control group.Results: All other parameters including area, thickness, diameter, and circumference of GDM placenta show a significant increase when compared with normal placenta. The gross anatomic features of placentae e.g infarcted areas, calcified areas and marginal insertion of the umbilical cord in the study group show significant increase in value (p>0.01) in diabetic and hypertensive groups when compared to that of the control or normal group.Conclusions: In present study we found that hypertensive placentae tend to be slightly smaller in size, weight, volume, area, thickness, diameter, circumference and feto-placental ratio than normal placentae but the parameters were found to be significantly greater than that of normal placentae in case of diabetic placentae. No significant differences were found in umbilical cord insertion. In normal pregnancy cases we found several histological findings which were increased in hypertensive and diabetic cases.

scholarly journals Influence of S(+)-ketamine analgesia in renal intraoperative ischemia: histological study in rats

2006 ◽  
Vol 21 (4) ◽  
pp. 242-246 ◽  
Author(s):  
Eloy Rusafa Neto ◽  
Pedro Thadeu Galvão Vianna ◽  
Rosa Marlene Viero ◽  
Norma Sueli Pinheiro Módolo ◽  
Eliana Marisa Ganem ◽  
...  
Keyword(s):  
Heart Rate ◽  
Arterial Pressure ◽  
Rectal Temperature ◽  
Wistar Rats ◽  
Histological Study ◽  
Sodium Pentobarbital ◽  
Histological Changes ◽  
Renal Histology ◽  
Male Wistar Rats ◽  
Arterial Hemorrhage

PURPOSE: To study in rats the effect of S(+)ketamine on the renal histology after intraoperative hemorrhage. METHODS: Twenty male Wistar rats, anesthetized with sodium pentobarbital, were randomly divided in 2 groups: G1 - control (n=l0) and G2 - S(+)-ketamine (n=10), both submitted to arterial hemorrhage of 30% of volemia in 3 moments (10% each 10 min) 60 min after anesthesia. G2 received S(+)-ketamine, 15 mg. kg-1, i.m., 5 min after anesthesia and 55 min before the 1st hemorrhage moment (Ml). Medium arterial pressure (MAP), rectal temperature (T) and heart rate were monitored. The animals were sacrificed in M4, 30 min after the 3rd hemorrhage moment (M3) and the kidneys and blood collected from hemorrhage were utilized for histological study and hematocrit (Ht) determination. RESULTS: There were significant reduction of MAP, T, and Ht. The histological study verified G1 = G2 for tubular dilation, congestion, and necrosis. The total score addition were significant1y different and G2 > G 1. CONCLUSION: Hemorrhage and hypotension determined changes in kidney histology. The rise in catecholamine blood concentration probably was the cause of S(+)-ketamine-induced higher score of histological changes.

scholarly journals Functional Changes in Thalamic Relay Neurons after Focal Cerebral Infarct: A Study of Unit Recordings from VPL Neurons after MCA Occlusion in Rats

1992 ◽  
Vol 12 (6) ◽  
pp. 954-961 ◽  
Author(s):  
Tatsuya Tokuno ◽  
Kazuo Kataoka ◽  
Toshiharu Asai ◽  
Shiko Chichibu ◽  
Ryotaro Kuroda ◽  
...  
Keyword(s):  
Firing Rate ◽  
Histological Study ◽  
Histological Changes ◽  
Functional Changes ◽  
Mca Occlusion ◽  
Thalamic Relay ◽  
Somatosensory Pathway ◽  
Repetitive Electrical Stimulation ◽  
Thalamic Relay Neurons ◽  
Stimulation Of

We evaluated neuronal and histological changes of thalamic neurons 1, 4, 7, and 14 days after middle cerebral artery (MCA) occlusion in rats. After the somatosensory evoked potentials (SEPs) were measured from the cerebral cortex, the thalamic relay neuronal activities were recorded with a glass microelectrode following repetitive electrical stimulation of the contralateral forepaw at frequencies ranging from 1 to 50 Hz. In ∼95% of the occluded rats, the ipsilateral somatosensory cortex and/or the subcortical somatosensory pathway developed infarct, resulting in SEP loss. We evaluated unit data from rats with abolished SEPs. The average firing rate of the nucleus ventralis posterolateralis (VPL) neurons in response to 25 stimulations at 30 Hz was significantly reduced to 0.1 spike/stimulus 1 day after MCA occlusion. In sham-operated rats, the same stimulation produced 0.7 spike/stimulus. The firing rate recovered to 0.4 spike/stimulus at 30-Hz stimulation 4 and 7 days after occlusion. This was followed by resuppression (0.1 spike/stimulus) 14 days after occlusion. Histological study revealed some abnormal neurons in the ipsilateral thalamus 7 days after occlusion. We were unable to find normal-shaped neurons in the VPL 14 days after occlusion. The present study demonstrates that cortical infarct produces functional and morphologic changes that gradually and progressively affect the ipsilateral thalamus, although incomplete transient recovery of somatosensory transmission may occur.

scholarly journals Efficacy Of Bacillus Thuringiensis Var. Israelensis Against The Larvae Of Aedes Aegypti

2015 ◽  
Vol 41 (1) ◽  
pp. 33-44
Author(s):  
Murshida Begum ◽  
Homaira Akbar ◽  
Mihir Lal Saha ◽  
Humayun Reza Khan
Keyword(s):  
Bacillus Thuringiensis ◽  
Histological Study ◽  
Aqueous Suspension ◽  
Fluorescent Microscopy ◽  
Control Sample ◽  
Bacterial Spores ◽  
Histological Changes ◽  
Bacterial Cultures ◽  
Microscopic Studies ◽  
Microscopic Techniques

Efficacy of aqueous suspension of Bacillus thuringiensis var. israelesis (Bti) was studiedagainst the laboratory reared 3rd and 4th instar larvae of Aedesaegypti. Mortality rates aswell as histological changes in the larval midgut due to toxic effect of Bti wereinvestigated. Several microscopic techniques were also used to identify the toxic effecton the mosquito larvae. The highest mortality rate (96.66%) was found in case of 3rdinstar larvae at 1.0μl/ml dose where LC50 value was 0.0097. Larval mortalities increasedsignificantly to A. aegypti as doses increased (p< 0.05). Histological study revealed thatcellular layers of the midgut epithelium were intact in control sample, but in case of theBti treated larvae none of these were found in the midgut, only bacterial spores wereseen. Results of the microscopic studies indicated that, among the six different coloniesfound in bacterial cultures, Bti spore, sporangium and vegetative cells were confirmedfrom one colony by phase transition and fluorescent microscopy. The Cry (crystals)endotoxins and Bti spore were confirmed by the SEM. Asiat. Soc. Bangladesh, Sci. 41(1): 33-44, June 2015

scholarly journals Some Aspects of Reproduction in Long Whiskered Catfish, Sperata aor (Hamilton 1822), from North-East Bangladesh

2021 ◽  
Vol 21 (2) ◽  
pp. 47-54
Author(s):  
Md. Naimuddin Jabed ◽  
Mohammad Amzad Hossain ◽  
Sohel Mian ◽  
Muhammad Anamul Kabir ◽  
Sabuj Kanti Mazumder ◽  
...  
Keyword(s):  
Developmental Stages ◽  
Histological Study ◽  
Gonadosomatic Index ◽  
Morphological Characteristics ◽  
Female Fish ◽  
Histological Changes ◽  
Average Value ◽  
Absolute Fecundity ◽  
North East ◽  
One Year

Different reproductive aspects such as sexual maturity, spawning, gonadosomatic index, fecundity, and histological changes in gonads of captive reared Sperata aor were investigated for a period of one year. Male and female fish were identified by based on morphological characteristics. The spawning season was found to be extended from May-August. Gonadosomatic index reaches maximum in August and lowest in September. Absolute fecundity varied from 59255 to 70586 with an average value of 64920. Absolute fecundity had been reported to be increased with total length, body weight. Histological study of ovary of S. aor indicated the presence of four developmental stages viz, early perinucleolar s oocytes, late perinucleolar oocytes, yolk vesicle stage and yolk granular stage. Findings reveal that, S. aor has group asynchronous manner of ovarian growth and used to spawn numerous times in a year under favorable environmental conditions. The testes histology represents the presence of spermatocyte, spermatid, and spermatozoa. This species builds nests during breeding season and fries are found within the nests.

scholarly journals Effect of vydate gill histology in female mosquito fish Gambusia affinis (Baird and Girard)

2017 ◽  
Vol 14 (1) ◽  
pp. 1-10
Author(s):  
Baghdad Science Journal
Keyword(s):  
Histological Study ◽  
Gambusia Affinis ◽  
Chloride Cells ◽  
Control Group ◽  
University Campus ◽  
Histological Changes ◽  
Histological Alterations ◽  
Mosquito Fish ◽  
Secondary Lamellae ◽  
Gill Lamellae

This study aimed to identify the histological changes of mosquito fish (Gambusia affinis) exposed to vydate pesticide. (48) female fishes were collected from local markets and also from the stream that surround Baghdad university campus at Al-jadyria, fishes divided into (4) groups and each group consist of 12 fish: T1(exposed to 0.0000001 mg/L),T2 (exposed to 0.0000002 mg/L ) and T3 (exposed to 0.000001 mg /L) of vydate pesticide for (8) weeks. As well as a control group. Fishes were dissected for histological preparations after each (2,4,6,8) weeks of exposure time. Gills were fixed by Bouin’s fluid and stained with Hematoxylin and eosin. Histological study showed that the structure of gill in a control group consists of primary and secondary gill lamellae appeared parallel and oblique. The secondary gill lamellae lined with squamous epithelial and pillar cells, and each secondary lamellae have capillary derived from blood vessel in primary lamellae, chlorid cells and few mucous cells found between secondary lamellae. The histological alterations in gills included hyperplasia of chloride cells, congestion of blood vessels, lamellar disarray, lifting of epithelial cells, Oedema, fusion of adjacent secondary lamella and Aneurysm. Results concluded that vydate had a vicious effect on gills and caused severe histological alterations such as hyperplasia of chloride cells and simple alterations such as lamellar disarray, and also these histological changes commensurate direct proportion with concentrations were used in this study.

746 EXPERIENCE IN RECONSTRUCTION OF LARYNGOPHARYNGOESOPHAGECTOMIES IN A THIRD LEVEL CENTER

2021 ◽  
Vol 34 (Supplement_1) ◽  
Author(s):  
Ana Navío-Seller ◽  
David Abelló-Audí ◽  
Marcos Bruna-Esteban ◽  
Javier Vaqué-Urbaneja ◽  
Fernando Mingol-Navarro ◽  
...  
Keyword(s):  
Hospital Stay ◽  
Functional Result ◽  
Neoadjuvant Treatment ◽  
Oral Intake ◽  
Functional Results ◽  
Morbidity And Mortality ◽  
High Morbidity ◽  
Pharyngoesophageal Reconstruction ◽  
Free Grafts ◽  
Pharyngeal Reconstruction

Abstract   Pharyngoesophageal reconstruction after laryngo-pharyngo-esophagectomy, due to malignant or benign causes, is challenging due to its high morbidity and mortality. There are different reconstructive flaps: visceral flaps (pedicle stomach and colon flaps and free jejunum or colon grafts) and myocutaneous flaps (pedicle local flaps, such as the pectoralis major flap, or free grafts, such as the anterolateral thigh-ALT). The objective is to evaluate the morbidity and mortality and functional results of the reconstruction after laryngo-pharyngo-esophagectomy. Methods This is a retrospective study of patients who underwent laryngo-pharyngo-esophagectomy in our center, due to a benign cause (ingestion of caustic) or malignant (cancer of the larynx, pharynx, parathyroid and cervical esophagus) with circumferential pharyngeal reconstruction with flap, from 2008 to November 2020. Demographic variables, neoadjuvant treatment, procedure performed and flap used for reconstruction, complications related to reconstruction (fistula, stenosis, necrosis), postoperative complications, days until adequate swallowing, functional result of the flap, hospital stay, recurrence and mortality were collected. Results Twelve patients, with a median age of 59 years (45–78), underwent surgery, 1 case due to benign cause and 11 cases with an oncological diagnosis. There were complications related to the reconstruction in 42% of the patients (see table 1). Postoperative morbidity was 67% (75% Clavien-Dindo ≥ III). The median hospital stay was 21 days (16–94). The median time to swallowing was 13 days (3–73). An optimal functional result (oral intake) was achieved in 75% (only 3 patients with poor results). The median follow-up was 18 months (4–56), with a survival rate of 50%. 30-days mortality was 8% (1 case). Conclusion Our study shows a high morbidity and mortality after circumferential pharyngeal reconstruction, similar to literature published. We have observed a higher rate of reconstruction related complications (fistulas and stenosis) and worse functional results in reconstructions performed with gastroplasty and coloplasty after total laryngo-pharyngo-esophagectomy, compared to less aggressive local resections (laryngopharyngeal) with ALT free flap reconstruction.

scholarly journals Histological study on acute inflammatory reaction to polyurethane-coated silicone implants in rats

2008 ◽  
Vol 23 (1) ◽  
pp. 93-101 ◽  
Author(s):  
Paulo Roberto da Silva Mendes ◽  
Jorge Bins-Ely ◽  
Eduardo Arnaut dos Santos Lima ◽  
Zulmar Antonio Accioli de Vasconcellos ◽  
Armando José d'Acampora ◽  
...  
Keyword(s):  
Inflammatory Reaction ◽  
Staphylococcus Epidermidis ◽  
Histological Study ◽  
Giant Cells ◽  
Group Iv ◽  
Silicone Implants ◽  
Histological Changes ◽  
Group Iii ◽  
Discriminating Power ◽  
Bacterial Concentrations

PURPOSE: Evaluating histologically the silicone peri-implant coated by polyurethane inflammation associated to the use of anti-microbial and bacterial contamination. METHODS: It was used 35 Wistar rats. The animals were divided in seven groups: I - Control; II - implant cavity contamination with10 bacteria/ml; III - implant cavity contamination with 10 bacteria/ml; IV - implant cavity contamination with 10 bacteria/ml; V - identical contamination to group II and implant immersions in anti-microbial solution; VI - identical contamination in group III and implant immersions in the anti-microbial solution; VII - identical contamination of group IV and implant immersions in anti-microbial solution. It was evaluated morphometrically the peri-implant capsules after 30 days of introduction. RESULTS: The factors with more discriminating power were the giants cells of a strange body and the mononuclear. There was no correlation between the bacterial concentrations and the histological alterations. CONCLUSION: 1) The histological standard of the inflammatory reaction around the silicone implant coated with polyurethan is chronic granulomatosis type of a strange body; 2) There isn´t correlation between concentration of Staphylococcus epidermidis and histological changes; 3) The use of anti-microbial solution decreased the mononuclear cell reactions, with the increase of giant cells in a strange body.

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