scholarly journals Role of wild type p53 and double suicide genes in interventional therapy of liver cancer in rabbits

2012 ◽  
Vol 27 (8) ◽  
pp. 522-528 ◽  
Author(s):  
Hong-xin Niu ◽  
Tong Du ◽  
Zhong-fa Xu ◽  
Xi-kun Zhang ◽  
Ruo-gu Wang
Keyword(s):  
Gene Therapy ◽  
Liver Cancer ◽  
P53 Gene ◽  
Interventional Therapy ◽  
Inhibitory Effect ◽  
Cancer Growth ◽  
Tumor Growth Rate ◽  
Significant Difference ◽  
Group 5 ◽  
Group 2

PURPOSE: To investigate the feasibility of interventional lipiodol embolism and multigene therapy in combination with focal chemotherapy in the treatment of VX2 liver cancer in rabbits. METHODS: Forty five rabbits with cancer larger than 2cm in diameter were randomly divided into five groups (n=9 per group). In Group 1, animals were treated with 0.9% sodium chloride. In Group 2, animals received lipiodol embolism. In Group 3, animals received lipiodol embolism and p53 gene therapy. In Group 4, animals received lipiodol embolism and TK/CD gene therapy. In Group 5, animals received lipiodol embolism and p53 and TK/CD gene therapy. Ultrasonography and CT were performed before and at ten days after interventional therapy. RESULTS: The VX2 model of liver cancer was successfully established in rabbits and interventional therapy smoothly performed. At ten days after interventional therapy, significant difference in the tumor volume was noted among five groups (p<0.05) and different treatments could inhibit the cancer growth. The inhibition of cancer growth was the most evident in the Group 5. Factorial analysis revealed gene therapy with p53 or TK/CD and lipiodol embolism independently exert significantly inhibitory effect on cancer growth. In addition, the suppression on tumor growth rate was the most obvious in the Group 5. CONCLUSIONS: Combination of gene therapy with lipiodol embolism can effectively inhibit the cancer growth and prolong the survival time. These findings demonstrate the effectiveness of multigene therapy in combination with lipiodol embolism in the treatment of liver cancer.

In vivosilymarin’s antioxidant and anti-apoptotic effects on photodynamic therapy’s responsiveness

2017 ◽  
Vol 21 (03) ◽  
pp. 189-197 ◽  
Author(s):  
Vlad Dionisie ◽  
Simona Clichici ◽  
Rodica M. Ion ◽  
Oana O. Danila ◽  
Remus Moldovan ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Reactive Oxygen Species Generation ◽  
Group 4 ◽  
Significant Difference ◽  
Anti Oxidant ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Apoptotic Effects ◽  
Oxidant Effect

Several studies have shown that some anti-oxidant natural compounds in combination with photodynamic therapy (PDT) can enhance the effectiveness of treatment. The aim of this study is to evaluate the effect of silymarin (SIL) in combination with 5,10,15,20-tetra-sulphonato-phenyl-porphyrin (TSPP) based photodynamic therapy, on experimental tumors. 30 Wistar rats with Walker carcinosarcoma, were divided into 6 groups: group 0 (control) — control, untreated group; group 1 (TSPP) — one dose of TSPP; group 2 (SIL) — silymarin; group 3 (PDT) — TSPP and irradiation 24 h after; group 4 (SIL[Formula: see text]PDT) — silymarin, TSPP and irradiation 24 h after; group 5 (SIL[Formula: see text]IR) and group 6 (IR) — irradiation and in addition, group 5 received SIL. Silymarin administered before photodynamic therapy decreased the lipid peroxidation ([Formula: see text] < 0.05) and modulated the antioxidant defense in tumor treated with PDT and silymarin suggesting that silymarin administration along with photodynamic therapy has an anti-oxidant effect. The caspase — 8 level and -3 activity increased in PDT and PDT [Formula: see text] SIL groups compared to the control; between the two groups there was a significant difference in term of apoptosis in favor to PDT. In conclusion, silymarin administration inhibited the reactive oxygen species generation and reduced the tumoral cells’ apoptosis, suggesting that natural compound administered before photodynamic therapy did not improve the therapy’s effect.

scholarly journals The Relationship between First Trimester 25-Hydroxyvitamin D3 Levels and Second Trimester Femur Length and Their Effects on Birth Weight and Length at Birth: A Preliminary Study

2019 ◽  
Vol 2019 ◽  
pp. 1-4
Author(s):  
Elif Ganime Aydeniz ◽  
Umut Sari ◽  
Isil Tekin ◽  
Talat Umut Kutlu Dilek
Keyword(s):  
Vitamin D ◽  
Birth Weight ◽  
Serum Vitamin ◽  
First Trimester ◽  
Serum Vitamin D ◽  
Vitamin D Levels ◽  
Significant Difference ◽  
Group 5 ◽  
Group 2 ◽  
Term Births

Objective. The main goal of our study was to assess relationships between first trimester 25-hydroxyvitamin D3 levels and infant birthweight and length at birth. Materials and Methods. We conducted a study over our medical records of 154 live-term births at Acibadem Atakent Hospital, Istanbul, Turkey. Subjects were classified into five independent groups. Results. We retrospectively reviewed a total of 154 live birth records. They took vitamin D3 supplement 1000 U/day. We classified the serum vitamin D levels into 5 groups by concentration. Group 1 comprised serum vitamin D levels <10 ng/ml (n = 41); group 2 comprised serum Vitamin D levels between >10–16 ng/ml (n = 33); group 3 comprised serum vitamin D levels >16–20 ng/ml (n = 26); group 4 vitamin D level between >20–30 ng/ml (n = 33) and group 5 comprised vitamin D levels >30 ng/ml. The femurs of infants were found to be longer between the groups, although the differences were not significant (p=0.054). There was also a statistically significant difference in the neonatal birth weight (p=0.048). Conclusion. We observed associations between low and high maternal 25-hydroxyvitamin D3 levels and fetal growth at birth weight but no difference in birth length. We conclude that we always need to conduct further research to be able to predict the effects of vitamin D deficiency.

scholarly journals Evaluation of Apical Filling after Warm Vertical Compaction using Two Different Endodontic Materials: Resilon® and Gutta-Percha

2012 ◽  
Vol 3 (2) ◽  
pp. 166-170
Author(s):  
Carla Zogheib ◽  
Etienne Medioni
Keyword(s):  
Group 4 ◽  
Gutta Percha ◽  
Anterior Teeth ◽  
Significant Difference ◽  
Ah Plus ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Warm Vertical Compaction ◽  
Group 1

ABSTRACT Forty-eight extracted maxillary anterior teeth instrumented with ProTaper system to apical preparations 0.25, 0.40 and 0.60 mm (n = 16 each) were divided into six groups for filling as following: Group 1: Apical preparation 25/100 + gutta-percha/AH Plus sealer, group 2: Apical preparation 25/100 + Resilon/Real Seal, group 3: Apical preparation 40/100 + gutta-percha/AH plus sealer, group 4: Apical preparation 40/100 + Resilon/Real Seal, group 5: Apical preparation 60/100 + gutta-percha/AH plus, group 6: Apical preparation 60/100 + Resilon/Real Seal. All teeth were filled using the warm vertical technique. Horizontal sections at 1, 2 and 3 mm from the apex were observed by SEM under 200 × magnification. Sections were digitally photographed under a stereomicroscope and the images were transferred to a compatible PC for image analysis. The surface area of voids were calculated and compared at all levels; using the Kruskal-Wallis test with Sidak correction and Mann-Whitney U test (p > 0.05). Comparisons within each group (gutta-percha/AH plus and Resilon/Real Seal) showed the presence of voids but there was no significant difference between any level of sectioning for apical preparations of 25 and 40 (p > 0.05). The only difference was in the groups prepared to size 60 and filled with Resilon/ Real Seal which showed significantly more voids than the group filled with gutta-percha/AH plus and especially at the 3 mm level. Conclusion The system Resilon/Real Seal did not achieve better results in terms of sealing ability in the apical third when compared to the conventional gutta-percha/AH plus sealing system. How to cite this article Zogheib C, Naaman A, Medioni E. Evaluation of Apical Filling after Warm Vertical Compaction using Two Different Endodontic Materials: Resilon® and Gutta-Percha. World J Dent 2012;3(2):166-170.

scholarly journals The effects of selenium and vitamin E on lung tissue in rats with sepsis

2012 ◽  
Vol 35 (2) ◽  
pp. 48 ◽  
Author(s):  
Mustafa Atli ◽  
Mehmet Erikoglu ◽  
Adnan Kaynak ◽  
Haci H Esen ◽  
Sevil Kurban
Keyword(s):  
Vitamin E ◽  
Lung Tissue ◽  
Cecal Ligation ◽  
Sepsis Group ◽  
Control Group ◽  
Significant Difference ◽  
Intraperitoneal Dose ◽  
Group 5 ◽  
Pre Treatment ◽  
Group 2

Purpose: In this study we examined the ability of selenium and vitamin E to prevent sepsis-induced changes in lung tissue. Methods: Fifty rats were divided into five groups: Group 1: Control group; Group 2: Sepsis group. In this group only cecal ligation and perforation (CLP) was performed. Group 3: Selenium group. An intraperitoneal dose of 100 µg selenium was given for the first two days followed by a daily dose of 40 µg for the next five days. CLP was performed the following day. Group 4: Selenium and vitamin E group. In addition to selenium, vitamin E was given intramuscularly in a dose of 250 mg/kg/day for seven days. CLP was performed the following day. Group 5: Vitamin E group. Vitamin E was given intramuscularly in a dose of 250 mg/kg/day for seven days. CLP was performed the following day. Results: There were significant differences between Group 2 and all other groups in terms of blood gas values (pH, pCO2, SaO2), and leukocyte, C-reactive protein (CRP) and glutathione peroxidase levels (p < 0.005). There was no statistically significant difference between groups 3, 4 and 5 in terms of histopathological changes in lung tissue (p > 0.05), but all groups were significantly different compared with Group 2 (p < 0.05). Conclusion: Sepsis-induced lung tissue damage can be reduced or prevented by pre-treatment with of selenium and/or vitamin E in a rat model.

scholarly journals Comparing the early function and complications between fluoroscopic guidance and blindly insertion of permanent hemodialysis catheter

2018 ◽  
Vol 5 (1) ◽  
pp. 19-22
Author(s):  
Nasser Malekpour Alamdari ◽  
Samad Shams Vahdati ◽  
Barmak Gholizadeh ◽  
Shima Nayebian
Keyword(s):  
Vascular Injury ◽  
Fluoroscopic Guidance ◽  
Hemodialysis Catheter ◽  
Early Function ◽  
Catheter Exchange ◽  
Significant Difference ◽  
Electrolyte Homeostasis ◽  
The Difference ◽  
Group 5 ◽  
Group 2

Objective: Chronic kidney disease (CKD) is a complicated kidney defect causing permanent failure in renal function in progressive stages. Hemodialysis is the most accepted treatment to maintain body’s fluid/electrolyte homeostasis at the terminal stages of the disease. Permanent hemodialysis catheter (permicath) may be inserted blindly or by fluoroscopic guidance. This study aimed to compare the early function and complications between fluoroscopic guidance and blindly insertion of permanent hemodialysis catheter. Methods: This prospective randomized clinical trial was undertaken in the emergency department of Modarres hospital in Tehran, Iran during 2014 and 2015. Patients who needed catheter due to renal failure entered the study. Patients who needed emergency dialysis and those who could not wait for permicath were excluded. Patients were randomly assigned into 2 groups, under fluoroscopic guidance and blindly catheter insertion. Data were collected using a questionnaire and a checklist related to function (after 24 hours and 1 month), a need to exchange the catheter and the early adverse effects such as pneumothorax, hemothorax, and vascular injury. Results: A total of 101 patients were enrolled in this trial. Early dysfunction (blind group= 5), a need for catheter exchange (blind group= 2), pneumothorax (blind group=2), vascular injury (blind group=1) were recorded but the difference between the two groups was not statistically significant (P>0.05). Conclusion: We did not observe a significant difference between the placement of permicath by fluoroscopic or blind method. However, more studies with larger groups are recommended.

Blood Ca2+ modulates responsiveness of renal 25(OH)D3-1 alpha-hydroxylase to PTH in rats

1987 ◽  
Vol 253 (5) ◽  
pp. E503-E507 ◽  
Author(s):  
T. Matsumoto ◽  
K. Ikeda ◽  
K. Morita ◽  
S. Fukumoto ◽  
H. Takahashi ◽  
...  
Keyword(s):  
Base Line ◽  
Hydroxylase Activity ◽  
Dihydroxyvitamin D ◽  
Group 4 ◽  
Significant Difference ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Serum Inorganic Phosphate ◽  
Group 1

To clarify whether extracellular Ca2+ modulates renal 25-hydroxyvitamin D3 [25(OH)D3]-1 alpha-hydroxylase, thyroparathyroidectomized rats were infused with 15 mM CaCl2, 20 mM EGTA, and/or 2.5 U/h parathyroid hormone (PTH), and blood Ca2+, serum 1,25-dihydroxyvitamin D [1,25(OH)2D], and renal 1 alpha-hydroxylase activity were determined. Rats with CaCl2, EGTA, or PTH infusion (group 1) exhibited low blood Ca2+, serum 1,25(OH)2D, and 1 alpha-hydroxylase activities. Infusion of CaCl2 alone (group 2) caused a significant increase in blood Ca2+ and a reduction in serum 1,25(OH)2D and 1 alpha-hydroxylase compared with group 1. Administration of PTH alone (group 3) markedly elevated blood Ca2+, serum 1,25(OH)2D, and 1 alpha-hydroxylase activity. When EGTA was infused along with PTH (group 4), blood Ca2+ was significantly reduced compared with group 3, and serum 1,25(OH)2D and renal 1 alpha-hydroxylase were further elevated. In contrast, when CaCl2 was infused with PTH (group 5), blood Ca2+ was higher than that in group 3, and serum 1,25(OH)2D and 1 alpha-hydroxylase activities were significantly reduced compared with group 3. No significant difference in serum inorganic phosphate or urinary cAMP excretion was observed by CaCl2 or EGTA infusion in both PTH-treated and nontreated rats. These results demonstrate that extracellular Ca2+ modulates the responsiveness of renal 1 alpha-hydroxylase to PTH as well as the base-line activity of the enzyme in the absence of PTH. These effects of extracellular Ca2+ on renal 1 alpha-hydroxylase may serve to offer an efficient way of regulating 1,25(OH)2D production and serum 1,25(OH)2D concentration by altering the responsiveness of 1 alpha-hydroxylase to PTH and possibly other stimulations depending on the demand for Ca2+.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Feeding preferences of experienced and naïve goats and sheep for the toxic plant Ipomoea carnea subsp. fistulosa

2015 ◽  
Vol 45 (9) ◽  
pp. 1634-1640 ◽  
Author(s):  
Carlos Alberto Oliveira Júnior ◽  
Gabriela Riet-Correa ◽  
Everton Lima ◽  
Danilo Maia Leite ◽  
James A. Pfister ◽  
...  
Keyword(s):  
Cynodon Dactylon ◽  
Feeding Preferences ◽  
Ipomoea Carnea ◽  
Group 4 ◽  
Plant Group ◽  
Significant Difference ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Toxic Plant

<p><bold>Ipomoea carnea</bold> is a toxic plant that grazing goats and cattle may learn to ingest with repeated exposure. The objective of this study was to evaluate the feeding preferences of experienced and non-experienced (naïve) goats and sheep for<bold> I. carnea.</bold>The study used 3 groups of 5 goats (Group 1, experienced that were previously poisoned by the plant; group 2, naïve; Group 3, experienced eaters, composed of animals adapted to eat the fresh plant) and 2 groups of sheep (group 4, experienced that were previously poisoned by the plant; and group 5, naïve). For the test, the animals were placed daily for 10 minutes and 4 days in a rectangular stall (5x7m) with 4 feeders, each with 200g of a different food (<bold>Ipomoea carnea</bold>, commercial concentrate food, recently harvested green grass (mainly <bold>Brachiaria</bold> spp.), and <bold>Cynodon dactylon</bold>hay. The intake of concentrate food was significantly higher (P<0.05) than the consumption of green grass, hay and <bold>I. carnea</bold>. In a second 4 day trial, in which the commercial concentrate food was replaced by freshly harvested <bold>Amorimia septentrionalis</bold>, the ingestion of green grass (<bold>Brachiaria</bold> spp.) was significantly higher (P<0.05) than the consumption of other foods. In both trials there was no significant difference in food consumption between eaters and naïve animals. The results suggest that experienced or naïve sheep and goats do not prefer <bold>I. carnea</bold> when it is offered with other foods or forages, suggesting that animals will avoid the plant and not become poisoned if other food options are available.</p>

Speech Perception and Language Outcomes for Infants Receiving Cochlear Implants Before or After 9 Months of Age: Use of Category-Based Aggregation of Data in an Unselected Pediatric Cohort

2021 ◽  
pp. 1-17
Author(s):  
Shani Dettman ◽  
Dawn Choo ◽  
Agnes Au ◽  
Amy Luu ◽  
Richard Dowell
Keyword(s):  
Speech Perception ◽  
Cochlear Implants ◽  
Large Data ◽  
Data Sets ◽  
Significant Difference ◽  
Language Data ◽  
Criterion Referenced ◽  
Group 5 ◽  
Group 2 ◽  
Group 1

Purpose This retrospective study aimed to amass large data sets to enable statistical comparisons of communication outcomes for infants receiving cochlear implants (CIs) before 9 months of age compared to groups who received their first CI between 9 months and 3.5 years of age. Method Speech perception scores and experienced clinicians' observations were used to refine the Categories of Auditory Performance Index (CAPI), thus creating its revised version, namely, the CAPI-Revised (CAPI-R). Standardized and criterion-referenced language data were used to create the novel Categories of Linguistic Performance (CLIP). The association between CAPI-R and CLIP data at two time points post implant (at 2 years of device experience and at 5 years of age) was examined in a large unselected cohort stratified for age at first implant: before 9 months (Group 1), between 9 and 12 months (Group 2), between 13 and 18 months (Group 3), between 19 and 24 months (Group 4), between 25 and 30 months (Group 5), between 31 and 36 months (Group 6), and between 37 and 42 months (Group 7). Results CAPI-R medians were 5 at 2 years of device experience, and 6 at 5 years of age. At 2 years of device experience, there was no significant difference in CAPI-R medians for children who received their first CI before 9 months compared to all other age-at-implant groups. At 5 years of age, a significantly better CAPI-R median was demonstrated by Group 1 (CI before 9 months) compared to Groups 4, 5, 6, and 7. CLIP medians were 3 at 2 years of device experience, and at 5 years of age. At 2 years device experience, and at 5 years of age, the Group 1 CLIP medians were significantly better than later age-at-implant groups. Conclusion Median CAPI-R outcomes supported access to CIs before 18 months of age for speech perception, and median CLIP outcomes supported access to CIs before 9 months of age for optimum language development.

Abstract T P2: Critical Delays In Intra-Arterial Therapy and Opportunities for Improvement

Stroke ◽  
2014 ◽  
Vol 45 (suppl_1) ◽  
Author(s):  
Amir R Honarmand ◽  
Shyam Prabhakaran ◽  
Maryam Soltanolkotabi ◽  
Farnoosh Feiz ◽  
Ryan Beck ◽  
...  
Keyword(s):  
Arrival Time ◽  
Critical Time ◽  
Onset Time ◽  
Time Intervals ◽  
Significant Difference ◽  
Hospital Transfer ◽  
Group 5 ◽  
Group 2 ◽  
Hospital Transport ◽  
Key Steps

Background and purpose: It has been demonstrated that every 30-minute delay in time to revascularization is associated with 10% absolute decrease in the probability of a good outcome from intra-arterial therapy (IAT) in acute ischemic stroke (AIS) patients. We investigated the potential critical time intervals among patients with direct presentation compared to inter-hospital transfers to comprehensive stroke centers (CSCs) for IAT. Methods: We performed a retrospective review of consecutive AIS patients who underwent IAT at 4 institutions since 2012 and abstracted symptom onset time (SOT), first emergency department arrival time (EDAT), and time of groin puncture (GP). Patients were categorized into those who were transferred from outside institutions and those who presented directly to the CSCs (non-transferred). We compared times using Mann-Whitney tests and proportions using Fisher’s exact tests. Results: Fifty-six patients were studied (58% male, mean age 65.3 years) were studied, of which 34 (60.7%) patients were inter-hospital transfers. Of the studied time intervals, SOT to CSC arrival time in non-transferred group was significantly less than that of transferred group (78.1 vs. 271.1 min, respectively; p<0.001). Additionally, there was a trend towards reduced SOT to GP time in non-transferred group (251.4 vs. 308.5 min; p=0.059). No significant difference was observed in first EDAT to GP time between non-transferred and transferred group (199.2 vs. 215.7; p=0.11). In non-transferred group, 5 (23%), 14 (64%), and 3 (13%) patients and in transferred group 2 (6%), 26 (76%), and 6 (17%) patients achieved SOT to GP time of <3, 3-6, >6 hours, respectively (p=0.17). Additionally, first EDAT to GP time of <120 minutes was achieved in only 3 cases (5.3%). Conclusions: Both among direct referrals and inter-hospital transfer patients, onset and initial arrival to treatment times are far from optimal. Nearly 90% of the time from onset to treatment is accounted for prior to CSC arrival among transfers while nearly 70% of the time elapses at CSCs among non-transfers. There are considerable opportunities for reducing delays in several key steps in the process including early team activation, inter-hospital transport, and rapid multimodal imaging protocols.

The Inhibitory Ability of Orobanche Extract on Calcium Oxalate Lithiasis in Male Albino Rats

2020 ◽  
Author(s):  
A. M. Kamal ◽  
M. S. Taha
Keyword(s):  
Treatment Group ◽  
Ethylene Glycol ◽  
Kidney Tissue ◽  
Inhibitory Effect ◽  
Control Group ◽  
Albino Rats ◽  
Potential Candidate ◽  
Group 4 ◽  
Group 5 ◽  
Group 2

The present study aimed to evaluate the inhibitory effect of Orobanche extract in ethylene glycol induced nephrolithiasis. Thirty male albino rats were divided into five groups each group contains 6 animals, group (1) control group, group (2) animals were supplied with 0.75% ethylene glycol in drinking water, group (3) animals were administrated Orobanche extract 3g/kg orally, group (4) animals were administrated Cystone 500 mg/kg in addition to 0.75% ethylene glycol, group (5) animals were administrated Orobanche extract 3g/kg orally in addition to 0.75% ethylene glycol the experiment continued for 28 days. Serum and the kidney homogenates were analyzed for various biochemical parameters and urine was examined microscopically for crystals. Orobanche treatment group and Cystone treatment group significantly decreased phosphorus, Calcium and Oxalate in kidney tissue of nephrolithiasis rats and significantly decreased kidney and liver marker in serum of nephrolithiasis rats. Conclusion this result revealed that Orobanche extract could be a potential candidate for phytotherapy against nephrolithiasis.

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