scholarly journals Antimicrobial analysis of different root canal filling pastes used in pediatric dentistry by two experimental methods

2006 ◽  
Vol 17 (4) ◽  
pp. 317-322 ◽  
Author(s):  
Lilian de Fátima Guedes de Amorim ◽  
Orlando Airton de Toledo ◽  
Cyntia Rodrigues de Araújo Estrela ◽  
Daniel de Almeida Decurcio ◽  
Carlos Estrela
Keyword(s):  
Zinc Oxide ◽  
Root Canal ◽  
Agar Plate ◽  
Brain Heart Infusion ◽  
Experimental Methods ◽  
Pediatric Dentistry ◽  
Root Canal Filling ◽  
Exposure Test ◽  
Direct Exposure ◽  
Agar Diffusion Test

The objective of this study was to compare, by two experimental methods, the antimicrobial efficacy of different root canal fillingpastes used in pediatric dentistry. The tested materials were: Guedes-Pinto paste (GPP), zinc oxide-eugenol paste (OZEP), calcium hydroxide paste (CHP), chloramphenicol + tetracycline + zinc oxide and eugenol paste (CTZP) and Vitapex®. Fiven microbial strains (S. aureus, E. faecalis, P. aeruginosa, B. subtilis and C. albicans) obtained from the American Type Culture Collection were inoculated in Brain Heart Infusion (BHI) and incubated at 37ºC for 24 h. For the direct exposure test (DET), 72 paper points were contaminated with the standard microbial suspensions and exposed to the root canal filling pastes for 1, 24, 48 and 72 h. The points were immersed in Letheen Broth (LB), followed by incubation at 37ºC for 48 h. An inoculum of 0.1 mL obtained from LB was then transferred to 7 mL of BHI, under identical incubations conditions and the microbial growth was evaluated. The pastes showed activity between 1 and 24 h, depending on the material. For the agar diffusion test (ADT), 30 Petri plates with 20 mL of BHI agar were inoculated with 0.1 mL of the microbial suspension, using sterile swabs that were spread on the medium. Three cavities were made in each agar plate (total = 90) and completely filled with one of the filling root canal pastes. The plates were pre-incubated for 1 h at room temperature and then incubated at 37ºC for 24 to 48 h. The inhibition zone around each well was recorded in mm. The complete antimicrobial effect in the direct exposure test was observed after 24 h on all microbial indicators. All root canal filling materials induced the formation of inhibition zones, except for Vitapex® (range, 6.0-39.0 mm).

scholarly journals Antimicrobial effect of 2% sodium hypochlorite and 2% chlorhexidine tested by different methods

2003 ◽  
Vol 14 (1) ◽  
pp. 58-62 ◽  
Author(s):  
Carlos Estrela ◽  
Rosane Galhardo Ribeiro ◽  
Cyntia R.A. Estrela ◽  
Jesus Djalma Pécora ◽  
Manoel Damião Sousa-Neto
Keyword(s):  
Sodium Hypochlorite ◽  
Culture Medium ◽  
Agar Plate ◽  
Antimicrobial Effect ◽  
Control Group ◽  
Diffusion Test ◽  
Exposure Test ◽  
Agar Diffusion ◽  
Direct Exposure ◽  
Agar Diffusion Test

The objective of this study was to analyze the antimicrobial effect of 2% sodium hypochlorite (NaOCl) and 2% chlorhexidine (CHX) by agar diffusion test and by direct exposure test. Five microorganisms: Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, Bacillus subtilis, Candida albicans, and one mixture of these were used. These strains were inoculated in brain heart infusion (BHI) and incubated at 37ºC for 24 h. For the agar diffusion test (ADT), 18 Petri plates with 20 ml of BHI agar were inoculated with 0.1 ml of the microbial suspensions, using sterile swabs that were spread on the medium, obtaining growth in junction. Fifty-four paper disks (9 mm in diameter) were immersed in the experimental solutions for 1 min. Subsequently, three papers disks containing one of the substances were placed on the BHI agar surface in each agar plate. The plates were maintained for 1 h at room temperature, and then incubated at 37ºC for 48 h. The diameter of microbial inhibition was measured around the papers disks containing the substances. For the direct exposure test, 162 #50 sterile absorbent paper points were immersed in the experimental suspensions for 5 min, and were then placed on Petri plates and covered with one of the irrigant solutions, or with sterile distilled water (control group). After intervals of 5, 10 and 30 min, the paper points were removed from contact with the solutions and individually immersed in 7 ml of Letheen Broth, followed by incubation at 37ºC for 48 h. Microbial growth was evaluated by turbidity of the culture medium. A 0.1 ml inoculum obtained from the Letheen Broth was transferred to 7 ml of BHI, and incubated at 37ºC for 48 h. Bacterial growth was again evaluated by turbidity of the culture medium. Gram stain of BHI cultures was used for verification of contamination and growth was determined by macroscopic and microscopic examination. The best performance of antimicrobial effectiveness of NaOCl was observed in the direct exposure test, and of CHX was observed in the agar diffusion test. The magnitude of antimicrobial effect was influenced by the experimental methods, biological indicators and exposure time.

scholarly journals Antimicrobial Activity of Two Root Canal Filling Pastes in Deciduous Teeth: an in Vitro Study

2015 ◽  
Vol 17 (1) ◽  
pp. 5
Author(s):  
Andréia Bolzan de Paula ◽  
Janaina De Cássia Orlandi Sardi ◽  
Mariana Minatel ◽  
Regina Maria Puppin-Rontani
Keyword(s):  
Antimicrobial Activity ◽  
Root Canal ◽  
Antimicrobial Property ◽  
Brain Heart Infusion ◽  
In Vitro Study ◽  
Biological Properties ◽  
Deciduous Teeth ◽  
Root Canal Filling ◽  
Agar Diffusion Test

<p>Endodontic treatment in deciduous teeth is very important so that we can preserve the primary dentition and thus promote appropriate craniofacial development. However, the use of filling pastes that have some important biological properties for the preservation of the tooth is required. The objective this study was to evaluate the in vitro antimicrobial activity of two root canal filling pastes in deciduous teeth, Vitapex® and Calcipex®, on microbial species commonly found in endodontic infections (Candida albicans, Enterococcus faecalis, Streptococcus mutans, Streptococcus sanguinis, Escherichia coli and Staphylococcus aureus), using the agar diffusion test. The experiment was carried out on BHI (Brain Heart Infusion) plates in four equidistant points, which were immediately filled with the sealers Vitapex® and Calcipex®. The<br />chlorhexidine gluconate 1% (CHX) and distilled water were used as positive and negative controls, respectively. After incubation of plates at 37 °C for 24 h, the diameter of the growth inhibition zones around the wells was measured (in millimeters) with a digital caliper with reflected light. The test was performed in triplicate and data were submitted to ANOVA and Tukey’s test (α = 0.05). Both pastes had antimicrobial activity, but Calcipex® was more efficient for most strains. The halo formed in the experiments with S. mutans and S. sanguinis was similar for both pastes. This study suggests that Calcipex® had the highest antimicrobial property with respect to the microorganisms studied.</p>

scholarly journals Antimicrobial Effect of Conventional Root Canal Medicaments vs Propolis against Enterococcus faecalis, Staphylococcus aureus and Candida albicans

2012 ◽  
Vol 13 (3) ◽  
pp. 305-309 ◽  
Author(s):  
Sudha Mattigatti ◽  
Deepak Jain ◽  
P Ratnakar ◽  
Shridhar Moturi ◽  
Sarath Varma ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Agar Plate ◽  
Brain Heart Infusion ◽  
Antimicrobial Effect ◽  
P Value ◽  
Agar Diffusion Test ◽  
Post Hoc

ABSTRACT Aim To evaluate and compare antimicrobial effect of various root canal medicaments against Enterococcus faecalis, Staphylococcus aureus and Candida albicans. Materials and methods Six root canal medicaments: 2% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX) Calcium hydroxide (Ca(OH)2), EDTA, MTAD and propolis and three microorganisms: Staphylococcus aureus, Enterococcus faecalis and Candida albicans were used. These strains were inoculated in brain heart infusion (BHI) and incubated at 37 degrees C for 24 hours. For the agar diffusion test (ADT), petri plates with 20 ml of BHI agar were inoculated with 0.1 ml of the microbial suspensions, using sterile swabs that were spread on the medium, obtaining growth injunction. Paper disks were immersed in the experimental solutions for 1 minute. Subsequently, four papers disks containing one of the substances were placed on the BHI agar surface in each agar plate. The plates were incubated at 37°C for 48 hours. The diameter of microbial inhibition was measured around the papers disks containing the substances. One way ANOVA followed by Tukey's post-hoc test were used. p-value <0.05 was considered statistically significant. Results Propolis and other irrigants were found to be effective on C. albicans, S. aureus and E. faecalis. CHX and MTAD were found to be most effective amongst all the materials tested followed by propolis. Conclusion Propolis showed antimicrobial activity against E. faecalis, S. aureus, C. albicans. It appears that propolis is an effective intracanal irrigant in eradicating E. faecalis and C. albicans. Clinical significance Propolis is an effective intracanal irrigant in eradicating E. faecalis and C. albicans. It could be used as an alternative intracanal medicament. How to cite this article Mattigatti S, Jain D, Ratnakar P, Moturi S, Varma S, Rairam S. Antimicrobial Effect of Conventional Root Canal Medicaments vs Propolis Against Enterococcus faecalis, Staphylococcus aureus and Candida albicans. J Contemp Dent Pract 2012;13(3):305-309.

scholarly journals Antibacterial activity of root canal filling materials for primary teeth: zinc oxide and eugenol cement, Calen paste thickened with zinc oxide, Sealapex and EndoREZ

2009 ◽  
Vol 20 (4) ◽  
pp. 290-296 ◽  
Author(s):  
Alexandra Mussolino de Queiroz ◽  
Paulo Nelson Filho ◽  
Léa Assed Bezerra da Silva ◽  
Sada Assed ◽  
Raquel Assed Bezerra da Silva ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Root Canal ◽  
Primary Teeth ◽  
Bacterial Strains ◽  
Root Canal Filling ◽  
Filling Materials ◽  
Root Canal Filling Materials ◽  
Bacterial Growth Inhibition

This study evaluated in vitro the antibacterial activity of 4 root canal filling materials for primary teeth - zinc oxide and eugenol cement (ZOE), Calen paste thickened with zinc oxide (Calen/ZO), Sealapex sealer and EndoREZ sealer - against 5 bacterial strains commonly found in endodontic infections (Kocuria rhizophila, Enterococcus faecalis, Streptococcus mutans, Escherichia coli and Staphylococcus aureus) using the agar diffusion test (agar-well technique). Calen paste, 1% chlorhexidine digluconate (CHX) and distilled water served as controls. Seven wells per dish were made at equidistant points and immediately filled with the test and control materials. After incubation of the plates at 37oC for 24 h, the diameter of the zones of bacterial growth inhibition produced around the wells was measured (in mm) with a digital caliper under reflected light. Data were analyzed statistically by analysis of variance and Tukey's post-hoc test (?=0.05). There were statistically significant differences (p<0.0001) among the zones of bacterial growth inhibition produced by the different materials against all target microorganisms. K. rhizophila was inhibited more effectively (p<0.05) by ZOE, while Calen/ZO had its highest antibacterial activity against E. faecalis (p<0.05). S. mutans was inhibited by Calen/ZO, Sealapex and ZOE in the same intensity (p>0.05). E. coli was inhibited more effectively (p<0.05) by ZOE, followed by Calen/ZO and Sealapex. Calen/ZO and ZOE were equally effective (p>0.05) against S. aureus, while Sealapex had the lowest antibacterial efficacy (p<0.05) against this microorganism. EndoREZ presented antibacterial activity only against K. rhizophila and S. aureus. The Calen paste and Calen/ZO produced larger zones of inhibition than 1% CHX when the marker microorganism was E faecalis. In conclusion, the in vitro antibacterial activity of the 4 root canal filling materials for primary teeth against bacterial strains commonly found in endodontic infections can be presented in a decreasing order of efficacy as follows: ZOE>Calen/ZO>Sealapex>EndoREZ.

scholarly journals Success frequency of root canal filling materials most used in pediatric dentistry

2018 ◽  
Vol 3 (3) ◽  
pp. 2-8
Author(s):  
Mariana Coutinho SANCAS ◽  
Andrea Vaz Braga PINTOR ◽  
Aline Borburema NEVES ◽  
Laura Guimarães PRIMO
Keyword(s):  
Root Canal ◽  
Pediatric Dentistry ◽  
Root Canal Filling ◽  
Filling Materials ◽  
Root Canal Filling Materials

Evaluation of Antimicrobial Efficacy of Various Root Canal Filling Materials Used in Primary Teeth: A Microbiological Study

2007 ◽  
Vol 31 (3) ◽  
pp. 193-198 ◽  
Author(s):  
S Reddy ◽  
Y Ramakrishna
Keyword(s):  
Zinc Oxide ◽  
Calcium Hydroxide ◽  
Root Canal ◽  
Primary Teeth ◽  
Endodontic Treatment ◽  
Root Canal Filling ◽  
Primary Molar ◽  
Filling Materials ◽  
Root Canal Filling Materials ◽  
Antimicrobial Effectiveness

The primary goal of endodontic treatment in primary teeth is to eliminate infection, and to retain the tooth in a functional state until their normal exfoliation time without endangering the permanent dentition or the general health of the child. The complexity of the pulp canal system in primary teeth presents a discerning problem for chemo-mechanical preparation. One of the factors determining the success of endodontic treatment in infected primary teeth is the sealing material that should encompass among other factors a potent bactericidal effect and the capacity to resorb along with the roots of primary teeth. This study evaluated the antimicrobial effectiveness of 5 root canal filling materials and a negative control agent against 23 strains of bacteria isolated from infected root canals of primary molar teeth and 3 non standard bacterial strains using agar diffusion assay. The materials were Zinc oxide and Eugenol (ZOE), Zinc oxide-Eugenol and Formocresol (ZOE+FC), Calcium hydroxide and sterile water (CAOH+H2O), Zinc oxide and Camphorated phenol (ZO+CP), Calcium hydroxide and Iodoform (Metapex) and Vaseline (Control). All the materials except Vaseline showed varied antimicrobial activity against the test bacteria. The zones of inhibition were ranked into 4 inhibition categories based on the proportional distribution of the data. All the 26 bacterial isolates were classified under 4 groups based on Aerobic/Anaerobic and Gram positive/Gram negative. Statistical analysis was carried out to compare the antimicrobial effectiveness between materials tested with each of the bacterial groupings. ZOE+FC produced strong inhibition against most bacteria when compared to ZOE, ZO+CP and CAOH+H2O. Metapex and Vaseline were found to be non inhibitory

Comparative Evaluation of Antimicrobial Efficacy of Various Root Canal Filling Materials Along with Aloevera Used in Primary Teeth: A Microbiological Study

2013 ◽  
Vol 37 (3) ◽  
pp. 257-262 ◽  
Author(s):  
R Kriplani ◽  
N Thosar ◽  
M S Baliga ◽  
P Kulkarni ◽  
N Shah ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Zinc Oxide ◽  
Calcium Hydroxide ◽  
Root Canal ◽  
Sterile Water ◽  
Root Canal Filling ◽  
Primary Molar ◽  
Filling Materials ◽  
Root Canal Filling Materials ◽  
Antimicrobial Effectiveness

Aim: this study was conducted to evaluate the antimicrobial effectiveness of 6 root canal filling materials and a negative control agent against 18 strains of bacteria isolated from infected root canals of primary molar teeth using agar diffusion assay. Materials: Aloevera with sterile water, Zinc oxide and Eugenol, Zinc oxide-Eugenol with aloevera, Calcium hydroxide and sterile water, Calcium hydroxide with sterile water and aloevera, Calcium hydroxide and Iodoform (Metapex) and Vaseline (Control). MIC and MBC of aloevera was calculated. Results: All materials except Vaseline showed varied antimicrobial activity against the test bacterias. The zones of inhibition were ranked into 4 inhibition categories based on the proportional distribution of the data. All the 18 bacterial isolates were classified under 2 groups based on Gram positive and Gram negative aerobes. Statistical analysis was carried out to compare the antimicrobial effectiveness between materials tested with each of the bacterial groupings. Conclusion: Aloevera + Sterile Water was found to have superior antimicrobial activity against most of the microorganisms followed by ZOE + Aloevera, calcium hydroxide + Aloevera, ZOE, calcium hydroxide, Metapex in the descending order and Vaseline showed no inhibition.

scholarly journals Severe Root Resorption after Obturating a Primary Molar Using Zinc Oxide and Eugenol at Different Follow-up Periods – A Case Report

2020 ◽  
Vol 8 (C) ◽  
pp. 99-100
Author(s):  
Mona Abdel Rehim Wahby ◽  
Sherine Ezz Eldin Taha ◽  
Eman Sayed El Masry ◽  
Randa Youssef Abd Al Gawad
Keyword(s):  
Zinc Oxide ◽  
Case Report ◽  
Root Canal ◽  
Root Resorption ◽  
Primary Molars ◽  
Filling Material ◽  
Root Canal Filling ◽  
Primary Molar ◽  
External Root Resorption

AIM: The aim of this study is to present severe root resorption following obturation of the primary molar with zinc oxide eugenol (ZOE) at different follow-up periods. CASE REPORT: We present the case of a 6-year-old boy with decayed lower primary molars. Pulpectomy for pulpally involved primary molars was performed. ZOE has been used as root canal filling material and follow-up was done at 3, 6, and 9 months. CONCLUSION: External root resorption was detected at 3 months and the resorptive process continued till 9 months. This finding emphasizes the need for alternative filling material with less irritating properties.

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