scholarly journals Quantitative radiographic evaluation of periapical bone resorption in dog's teeth contaminated with bacterial endotoxin (LPS) associated or not with calcium hydroxide

2008 ◽  
Vol 19 (4) ◽  
pp. 296-300 ◽  
Author(s):  
Léa Assed Bezerra da Silva ◽  
Raquel Assed Bezerra da Silva ◽  
Luis Guilherme Siqueira Branco ◽  
Valéria Pontelli Navarro ◽  
Paulo Nelson-Filho
Keyword(s):  
Bone Resorption ◽  
Calcium Hydroxide ◽  
Saline Group ◽  
Lesion Size ◽  
Bacterial Endotoxin ◽  
Radiographic Evaluation ◽  
Periapical Lesion ◽  
Pulp Tissue ◽  
Group 4 ◽  
Group 2

The aim of this study was to quantify radiographically the periapical bone resorption in dogs' teeth contaminated with bacterial endotoxin (LPS), associated or not with calcium hydroxide. After pulp tissue removal, 60 premolars were randomly assigned to 4 groups and were either filled with LPS (group 1), filled with LPS plus calcium hydroxide (group 2) or filled with saline (group 3) for a period of 30 days. In group 4, periapical lesion formation was induced with no canal treatment. Standardized radiographs were taken at the beginning of the treatment and after 30 days and the Image J Program was used for measurement of periapical lesion size. Periapical lesions were observed in groups 1 (average of 8.44 mm2) and 4 (average of 3.02 mm2). The lamina dura was intact and there were no areas of periapical bone resorption in groups 2 and 3. It may be concluded that calcium hydroxide was effective in inactivating LPS, as demonstrated by the absence of apical periodontitis in the roots that were filled with bacterial endotoxin plus calcium hydroxide.

Evaluation of biochemical changes in dental tissues after different office bleaching methods

2018 ◽  
Vol 38 (4) ◽  
pp. 389-397 ◽  
Author(s):  
ES Karaarslan ◽  
ZC Özmen ◽  
F Aytac ◽  
AA Bicakci ◽  
M Buldur ◽  
...  
Keyword(s):  
Biochemical Changes ◽  
Pulp Tissue ◽  
Dental Tissues ◽  
Group 4 ◽  
Halogen Light ◽  
Halogen Light Source ◽  
Proteolytic Activities ◽  
Group 2 ◽  
Increasing Demand ◽  
Laser Bleaching

The color of the teeth is an important topic for many people and can be influenced by intrinsic and extrinsic stains. There is an increasing demand for whitening of the teeth year by year. The most popular way of whitening is “bleaching,” which is the result of the breakdown of pigments located in the enamel and/or the dentin, caused by reactive oxygen species (ROS) released from bleaching agents. These bleaching agents could increase matrix metalloproteinase (MMP)-mediated collagen degradation in dentin. The aim of this study was to compare biochemical changes and oxidative stress levels of the human premolar dentin–pulp complex after three different bleaching methods containing hydrogen peroxide (H2O2) bleaching agents. Individuals, whose first four premolars were extracted for orthodontic purposes, included into the study. Group 1—Laser: bleaching gel containing 46% H2O2 (LaserWhite20 whitening gel, Biolase Technology Inc., San Clemente, CA, USA) and a diode laser activation (Ezlase 940 nm system). Group 2: 35% H2O2 containing whitening gel (Whiteness HP Maxx, FGM) and halogen light source activation (Optilux 501, Kerr, Orange, CA, USA). Group 3: 35% H2O2 containing whitening gel (Whiteness HP Maxx, FGM). Group 4—Control: No whitening treatment. According to the test results, there were no significant differences among groups in the values of cathepsin B and MMP proteolytic activities ( p > 0.05). The total ROS values released from the dentin tissue were higher than those obtained from the pulp tissue ( p < 0.05). There were significant differences among the bleaching groups in the ROS values released from the dentin tissue.

Toxicity of bean cooking media containing EDTA in mice

2020 ◽  
Vol 36 (6) ◽  
pp. 436-445
Author(s):  
Sabry Ali El-Naggar ◽  
Karim Samy El-Said ◽  
Mona Elwan ◽  
Maysa Mobasher ◽  
Fotouh Mansour ◽  
...  
Keyword(s):  
Ethylenediaminetetraacetic Acid ◽  
White Blood Cells ◽  
Saline Group ◽  
Negative Control ◽  
Blood Levels ◽  
Group 4 ◽  
Liver And Kidney ◽  
Group 5 ◽  
Group 2 ◽  
Kidney Functions

The possible renal and hepatic toxicities of ethylenediaminetetraacetic acid (EDTA) in bean cooking media were studied using 100 male albino mice. Two sublethal doses of EDTA were used to explore their toxic effects; 20 mg/kg and 200 mg/kg, which corresponded to 1/100th and 1/10th of LD50, respectively. Accordingly, the toxicity study was performed using 50 mice, divided into five groups ( n = 10/group) as follows: group 1 (Gp1) served as a negative control and was orally administered normal saline; group 2 (Gp2) was administered the bean cooking medium; group 3 (Gp3) was administered EDTA (200 mg/kg); group 4 (Gp4) was administered bean cooking medium containing 20 mg/kg of EDTA; and group 5 (Gp5) was administered bean cooking medium containing 200 mg/kg of EDTA. The results showed no significant changes in liver and kidney functions in Gp2 while Gp3, Gp4, and Gp5 exhibited significant increases in adverse liver and kidney function markers. Hematocrit values were significantly decreased in Gp3 and Gp5, while the total white blood cells counts were significantly decreased in Gp3 and significantly increased in Gp5. The number of platelets was decreased in Gp3, Gp4, and Gp5. The blood levels of sodium (Na+), iron (Fe2+), and calcium (Ca2+) were decreased in Gp3, Gp4, and Gp5 due to the chelating effects of EDTA. The hepatic and renal architectures were disorganized in Gp3, Gp4, and Gp5 with some hemorrhagic manifestations in livers and kidneys of mice. These results demonstrate that EDTA in bean cooking is harmful in mice under the conditions of this study, and the potentially harmful effects in humans supports restricting its use.

Effect of Qmix and Other Irrigants on Dentin Adhesives in Pulp Chambers of Primary Teeth: SEM Study

2017 ◽  
Vol 41 (5) ◽  
pp. 363-367
Author(s):  
Sidharth Kapoor ◽  
Naveen Manuja ◽  
Seema Chaudhary ◽  
Harsimran Kaur ◽  
Chaitra TR ◽  
...  
Keyword(s):  
Normal Saline ◽  
Saline Group ◽  
Primary Molars ◽  
Pulp Chamber ◽  
Significance Level ◽  
Group 4 ◽  
Two Samples ◽  
Group 6 ◽  
Group 5 ◽  
Group 2

Objective: To compare the effect of different endodontic irrigants on microleakage of adhesives used within pulp chamber of primary molars. Study Design: 72 Primary molars were divided into 6 groups, according to 3 irrigants and 2 adhesives used. After de-roofing the pulp chamber, pulp was extirpated. In 36 samples, pulp chambers were bonded with Xeno V+ after irrigation with either QMix (Group1); 17%EDTA+5%NaOCl (Group2) or normal saline (Group3) and in other half samples, pulp chambers were bonded with SinglebondUniversal after irrigation with either QMix (Group 4); 17%EDTA+5% NaOCl (Group5) or normal saline (Group 6). All the samples were restored with Filtek Z350. Ten teeth from each group were assessed for dye penetration. Two samples in each group were viewed under scanning electron microscope. Data was statistically analyzed using Mann–Whitney and Kruskal Wallis tests at a significance level of P &lt; 0.05. Results: Mean microleakage scores were: Group 1-1.5±0.70, Group 2-1.6±.51, Group 3-2.4±0.96, Group 4-1.2±0.42, Group 5-1.2±0.42, Group 6-1.1±0.32. Conclusions: Irrigation with QMix significantly reduced the microleakage of XenoV+ but had no significant effect on microleakage of SinglebondUniversal. Irrigation with EDTA/NaOCl or QMix had no detrimental effect on the sealing ability of either of the adhesive tested.

scholarly journals Superoxide via Sp3 mechanism increases renal renin activity, renal AT1 receptor function, and blood pressure in rats

2018 ◽  
Vol 315 (5) ◽  
pp. F1478-F1483 ◽  
Author(s):  
Mohammad Saleem ◽  
Xitao Wang ◽  
Indira Pokkunuri ◽  
Mohammad Asghar
Keyword(s):  
Blood Pressure ◽  
Saline Group ◽  
Renin Activity ◽  
Lysine Acetylation ◽  
Group 4 ◽  
Biochemical Studies ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

We tested a hypothesis that superoxide, by inducing Sp3, increases renal renin activity, renal angiotensin II type 1 receptor (AT1R) function, and blood pressure (BP) in rats. Group 1 rats were treated with vehicle, saline. Group 2 rats were treated with superoxide dismutase (SOD) inhibitor diethylthiocarbamate (DETC). Group 3 rats were treated with DETC and an SOD mimetic, tempol. Group 4 rats were treated with tempol only. All four groups of rats were treated for 2 wk then anesthetized, and BP was recorded. Thereafter, diuresis and natriuresis in response to AT1R blocker candesartan were determined. When compared with vehicle rats, BP increased in DETC rats. The increased BP in DETC rats decreased with tempol. Diuresis and natriuresis in response to candesartan increased in controls, and this further increased in DETC rats and decreased with tempol. A second set of four groups of rats underwent the same treatment as above and were anesthetized, and their kidneys were obtained for biochemical studies. The levels of superoxide but not hydrogen peroxide increased, whereas SOD activities decreased further in the renal cortical tissues of DETC rats than vehicle rats. These effects were attenuated with tempol in DETC rats. Moreover, tissue renin activity and abundance of membranous AT1R proteins increased more in DETC rats than vehicle rats, and decreased with tempol in DETC rats. Furthermore, the levels of lysine-acetylated, but not serine-phosphorylated, Sp3 increased more in the nuclei of DETC rats than vehicle rats. The increased levels of Sp3 lysine acetylation decreased in DETC rats with tempol. Taken together, our results suggest that superoxide activates renal Sp3 via lysine acetylation increasing renin activity, AT1R function, and BP in rats.

scholarly journals Nutrient composition and ameliorative effects of Cocos nucifera products on Alloxan-induced diabetic wistar rats

2017 ◽  
Vol 5 (2) ◽  
pp. 149 ◽  
Author(s):  
Amadi Benjamin ◽  
Ogunka-Nnoka Charity ◽  
Amadi Peter ◽  
Ogaji Miebaka
Keyword(s):  
Cocos Nucifera ◽  
Saline Group ◽  
Diabetic Rats ◽  
Coconut Milk ◽  
Coconut Water ◽  
Group 4 ◽  
Ameliorative Effect ◽  
Group 5 ◽  
Group 2 ◽  
Alloxan Monohydrate

Using standard methods, this study investigated the ameliorative effects of coconut products during alloxan-induced diabetic conditions. Experimental animals were divided into five groups, group 1 served as normal control rats fed only rat chow and saline, group 2 were dia-betic control rats intraperitoneally treated with 150mg/kg body weight alloxan monohydrate, group 3 were diabetic rats orally treated with 4ml/day of coconut milk, group 4 were diabetic rats orally treated with 4ml/day of coconut water, and group 5 were diabetic rats orally treat-ed with 4ml/day of a mixture of coconut milk and coconut water. The coconut products had high moisture, fats, potassium, magnesium, and sodium contents. Coconut milk exhibited the most effective glucose lowering effect, and on the 21st day. The total cholesterol was com-pletely normalized on treatment with coconut milk after alloxan induced diabetes, while the administration of the mixture of coconut milk and water had a comparable effect to administering only coconut milk on HDL, LDL, and TG. The alloxan-induced derangements on SOD, catalase and GPx were completely normalized after the coconut milk administration, while the mixture of coconut milk and water restored only SOD and GPx, and coconut water, ineffective on most of the antioxidant enzymes. Coconut water was ineffective on the RBC and HB of diabetic rats, while coconut milk and the mixture of coconut milk and water showed the most hemato-ameliorative effect. This study has shown the effectiveness of coconut products in the management of diabetes, with coconut milk the most effective.

scholarly journals Elimination of intracanal infection in dogs' teeth with induced periapical lesions after rotary instrumentation: influence of different calcium hydroxide pastes

2006 ◽  
Vol 14 (3) ◽  
pp. 172-177 ◽  
Author(s):  
Janir Alves Soares ◽  
Mário Roberto Leonardo ◽  
Léa Assed Bezerra da Silva ◽  
Mário Tanomaru Filho ◽  
Izabel Yoko Ito
Keyword(s):  
Calcium Hydroxide ◽  
Wilcoxon Test ◽  
Significance Level ◽  
Periapical Lesions ◽  
Root Canals ◽  
Group 4 ◽  
Large Numbers ◽  
Microbiological Sample ◽  
Group 2 ◽  
Viridans Group Streptococci

The aim of this study was to evaluate the antiseptic efficacy of rotary instrumentation associated with calcium hydroxide-based pastes prepared with different vehicles and antiseptics. Chronic periapical lesions were experimentally induced in 72 premolar root canals of four dogs. Under controlled asepsis, after initial microbiological sampling (A1), the root canals were instrumented using the ProFile system in conjunction with 5.25% sodium hypochlorite and the intracanal medication was placed. Four experimental groups were formed according to the pastes used: group 1- Calen (n=18), group 2- Calen+CPMC (n=20), group 3- Ca(OH)2 p.a.+ anaesthetic solution (n=16) and group 4- Ca(OH)2 p.a.+ 2% chlorhexidine digluconate (n=18). After 21 days, the pastes were removed; the canals were emptied and 96 hours later a second microbiological sample was obtained (A2). The incidence of positive microbiological cultures and the number of cfus in stages A1 and A2 were compared statistically by the Wilcoxon test while the influence of the different treatments in intracanal infection was evaluated by Kruskal-Wallis test at 5% significance level (p<0.05). Large numbers of strict and facultative anaerobes, and viridans group streptococci were found in 100% of root canals of A1 samples. Among A2 samples, all treatments showed significant reduction of cfus and positive cultures (p<0.05), but only groups 3 and 4 showed 100% of root canals free of microorganisms. Rotary instrumentation plus NaOCl 5.25% associated with intracanal medication produced a drastic reduction or elimination of intracanal microbiota, whose performance was not influenced by the nature of the vehicle or the antiseptic added to the Ca(OH)2 p.a.

scholarly journals A comparative clinical study of bioceramic and calcium hydroxide based root canal sealer in the treatment of non-vital permanent tooth with periapical lesion

2021 ◽  
Vol 11 (2) ◽  
pp. 26-31
Author(s):  
Farzana Hoque Tanmi ◽  
Md Abdul Hannan Sheikh ◽  
Mozammal Hossain ◽  
SM Abdul Quader ◽  
Salahuddin Ahmed ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Root Canal ◽  
Standard Procedure ◽  
Lesion Size ◽  
Permanent Tooth ◽  
Permanent Teeth ◽  
Periapical Lesion ◽  
Root Canal Sealer ◽  
Anterior Teeth ◽  
Short Period

Introduction: The use of Bioceramic sealer in the obduration of the root canal system has been expected by many of the previous studies. However, the clinical outcome has not yet been established. Objective: To compare the effectiveness of bioceramic and calcium hydroxide based root canal sealer in treatment of non-vital permanent teeth with periapical lesion (Periapical periodontitis). Materials and Methods: A total 100 mature permanent anterior teeth were selected after clinical and radiological examination which had non-vital pulp with periapical lesion. Clinically pulp vitality test, palpation and percussion test was performed maintaining standard procedure. Following cavity preparation and biomechanical preparation, each canal was obturated either with bioceramic (Endosequence BC) or calcium hydroxide based sealer  (Sealapex, Kerr). All participants were evaluated immediately after obturation, at  3 and 6 months for the assessment of change in size of periapical lesion, condition of periodontal ligament space, lamina dura and incidence of post-operative pain, swelling. Statistical analysis was performed using Chi- square(X2) test and t-test. A value of p<0.05 was considered as statistically significant. Results: Bioceramic was more effective in reducing the lesion size than that of calcium hydroxide. At 6 months, the mean lesion size was reduced from 3.52±0.7 to 1.30±0.462 mm in Bioceramics and from 3.48±1.07 to 1.58± 0.498 mm in sealapex treated teeth.. Furthermore, 98% of bioceramic treated teeth and 94% of sealapex treated teeth showed absence of swelling. The differences between two groups were statistically significant (p<0.05). Conclusion: In this short period of study, Bioceramics based sealer seems to be more effective than calcium hydroxide based sealer in repair of periapical lesions of the nonvital teeth. Update Dent. Coll. j: 2021; 11(2): 26-31

Physicochemical Analysis of Dental and Household Hypochlorites

2021 ◽  
Author(s):  
Michele Méndez Velázquez
Keyword(s):  
Physicochemical Analysis ◽  
Pulp Tissue ◽  
Common Solution ◽  
Group 4 ◽  
All Solutions ◽  
Significant Difference ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Group 1

Aim: Sodium hypochlorite is the most common solution in the Endodontics field, however, NaOCl concentrations of commercial brands which are prepared for this purpose differ from their actual concentration. The aim of this study was to compare the physicochemical properties of dental and household hypochlorites. Materials and Methods: The solutions were divided in the following groups: Control: Clorarex 6%, Group 1: Viarzoni-T 5.25%, Group 2: Endo Chlord 3%, Group 3: Chlor- XTRA 6%, Group 4: Viarzoni-T (diluted from 5.25%), Group 5: Cloralex (diluted from 6%), Group 6: Chlor- Xtra (diluted from 6%). pH and temperature were measured by means of potentiometer, dilution test was performed using bovine tissue and exposed to each solution. For cytotoxicity assay, erythrocytes were exposed to each solution and stained with Sternheimer-Malbin solution. Results: Dissolution times showed a significant difference since the shortest time was Cloralex (6.6 ± 2.09). The solution with the highest alkalinity in the original concentration was Endo Chlord for dental use (11.7 ± 2.2). The solution with the highest alkalinity in the original concentration was Endo Chlord for dental use (11.7 ± 2.2) but it also was the solution with less cytotoxicity. Conclusion: Viarzoni-T was the only solution without any dissolving capacity, Cloralex, ChorlXtra and EndoChlord were capable of dissolving bovine pulp tissue effectively, reducing their concentration of Cloralex and ChlorXtra did not reduce the pH, but all solutions showed a cytotoxic effect on erythrocites.

scholarly journals Inflammatory stress and idiosyncratic drugs hepatotoxicity in rabbit

2013 ◽  
Vol 12 (2) ◽  
pp. 7
Author(s):  
H. T. Mohamed
Keyword(s):  
Liver Enzymes ◽  
Saline Group ◽  
Rabbit Serum ◽  
Inflammatory Reactions ◽  
Inflammatory Stress ◽  
Group 4 ◽  
Hepatotoxic Effect ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Idiosyncratic drug hepatotoxicity is none or time-related, unpredictable, occur infrequently and can be fatal. It was proposed that inflammatory or oxidative stress occurs randomly in patients even after asymptomatic incidence can precipitate drug hepatotoxicity. To measure the hepatotoxicity of diclofenac in rabbit serum following the incidence of inflammatory stress by lipopolysaccharide (LPS) and correlate this to various stress parameters as Malondialdehyde (MDA). 24 rabbits were divided into four groups (6 each) according to the type of treatment. Group 1: control (received normal saline). Group 2 received diclofenac sod. (5mg/kg, orally 3times daily for three days). Group 3: received lipopolysaccharide (150μg/kg, i.v, 24 hours before killing. Group 4: received diclofenac sod. + Lipopolysaccharide (5mg/kg orally + 150μg/kg, i.v 24 hours before killing). Then for each animal were measure, liver M D A, liver enzymes. LPS potentiated the hepatotoxic effect of diclofenac sod. The effect is mediated by oxidative and inflammatory reactions as demonstrated by increase in a liver tissue M D A.

scholarly journals The Effects of Leukocyte- and Platelet-Rich Plasma (L-Prp) and Pure Platelet-Rich Plasma (P-Prp) an a Rat Endometriosis Model

2019 ◽  
Author(s):  
Ali Doğukan Anğın ◽  
İsmet Gün ◽  
Önder Sakin ◽  
Muzaffer Seyhan Çıkman ◽  
Zehra Meltem Pirioğlu ◽  
...  
Keyword(s):  
Platelet Rich Plasma ◽  
Saline Group ◽  
Group 4 ◽  
Vascular Congestion ◽  
Significant Difference ◽  
Group 3 ◽  
Group 2 ◽  
The Relationship ◽  
Volume Difference ◽  
Group 1

AbstractOur aim was to investigate the effect of platelet-rich plasma (PRP) derivatives, which can be produced from the patient’s own blood and have minimal side effects, on endometriosis. To the best of our knowledge, this is the first study in the literature that studies the relationship between PRP and endometriosis. Endometriosis foci were created in the first operation. In the second operation (30th day) groups were formed. Group 1 (n= 8) was administered saline, group 2 (n= 7) leukocyte- and platelet-rich plasma (L-PRP), and group 3 (n= 8) pure platelet-rich plasma (P-PRP). Group 4 (n= 10) was used to obtain PRP. In the last operation (60th day), the endometriotic foci were measured, and then excised. There was no statistically significant difference between the pre and post volumes of the endometriotic foci, between their volume differences and volume difference rates (p > .05). However, it was observed that existing implant volumes in all groups decreased statistically significantly within their own groups by the end of the experiment compared to the previous volumes (p < .05). When the implants were assessed through histopathological scoring in terms of edema, vascular congestion, inflammatory cell infiltration, hemorrhage, epithelial line, and hemosiderin accumulation and immunohistochemical staining in terms of VEGF, there was no significant difference in the comparison between the groups. Although L-PRP and P-PRP generated more reduction in the endometriosis foci, they did not create any statistical differences.

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