scholarly journals Growth response of Aspergillus flavus IMS1103 isolated from poultry feed

2016 ◽  
Vol 2 (2) ◽  
pp. 221-228 ◽  
Author(s):  
Monzur Morshed Ahmed ◽  
Md Fakruddin ◽  
Md Nur Hossain ◽  
Khandaker Rayhan Mahbub ◽  
Abhijit Chowdhury
Keyword(s):  
Growth Rate ◽  
Aspergillus Flavus ◽  
Growth Response ◽  
Culture Media ◽  
Agar Medium ◽  
Lag Time ◽  
Potato Dextrose Agar ◽  
Poultry Feed ◽  
Effect Of Temperature ◽  
Temperature Range

Aspergillus flavus strains were isolated from locally available poultry feeds. Effect of temperature, pH and culture media on growth of Aspergillus flavus was studied. Temperature ranged from 4-42°C (4, 10, 20, 25, 30, 37 and 42°C) was examined. Except for 4°C and 10°C, the isolate was able to grow for the whole temperature range. The growth was maximum at 25°C and was influenced with increasing or decreasing of temperature from 42°C to 20°C.The lag time was strongly influenced by the temperature at lower temperature level than at higher temperature range. Effect of pH on growth of Aspergillus flavus was also examined; from comparison of 3 different pH levels, it is concluded that at most temperatures pH 6.5 showed a higher growth rate and as a consequence required a shorter time to achieve maximum colony diameter. No significant variations in the lag time were observed. A natural poultry feed meal agar medium (FMAM) was developed in the laboratory and growth of A. flavus was compared with other 2 synthetic dehydrated media namely; Czapek’sdox Agar (CDA) and potato dextrose Agar (PDA). Poultry feed meal agar medium showed better growth response than Czapek’sdox agar and potato dextrose agar at all conditions. At 25°C and pH 6.5 found optimum for growth of Aspergillus flavus in feed meal agar medium whereas, temperature 30°C and pH 6.5 found optimum for growth for Czapek’sdox agar media and temperature 30°C and pH 6 showed high growth rate on potato dextrose agar. Poultry feed meal media showed high affinity for growth of mycelium and early spore formation than other media examined.Asian J. Med. Biol. Res. June 2016, 2(2): 221-228

scholarly journals Growth and sporulation of Metarhizium flavoviride var. Flavoviride on culture media and lighting regimes

2001 ◽  
Vol 58 (3) ◽  
pp. 613-616 ◽  
Author(s):  
Sideney Becker Onofre ◽  
Cindia Mara Miniuk ◽  
Neiva Monteiro de Barros ◽  
João Lúcio Azevedo
Keyword(s):  
Biological Control ◽  
Mycelial Growth ◽  
Culture Media ◽  
Agar Medium ◽  
Potato Dextrose Agar ◽  
Continuous Illumination ◽  
Agricultural Pests ◽  
Dark Cycle ◽  
Metarhizium Flavoviride ◽  
Conidia Production

Entomopathogenic fungi from the genus Metarhizium are largely used for the biological control of agricultural pests by conidia spreading on the field. Although conidia production is well studied in M. anisopliae, only few research studies were done in M. flavoviride. The present work was carried out alming to evaluate the Mycelial growth and sporulation of the entomopathogenic fungus Metarhizium flavoviride var. flavoviride growing at 27 ± 2°C on Potato-dextrose-agar (PDA), Czapek-agar (CZP) and a complete agar medium (CM) under three lighting regimes, (continuous illumination, light/dark cycle and an black light/dark cycle) were investigated. A completely randomized 3 × 3 (culture media × lighting regime) factorial design with four replicates was used. The best mycelial growth and sporulation occurred on the PDA and CM media under continuous illumination (P <= 0,05).

scholarly journals Growth Supplements for Helicobacter pylori

2000 ◽  
Vol 38 (5) ◽  
pp. 1984-1987 ◽  
Author(s):  
Xiuping Jiang ◽  
Michael P. Doyle
Keyword(s):  
Helicobacter Pylori ◽  
Ferrous Sulfate ◽  
Growth Response ◽  
Culture Media ◽  
Horse Serum ◽  
Brain Heart Infusion ◽  
Lag Time ◽  
Inhibitory Effects ◽  
Sodium Pyruvate ◽  
H Pylori

The growth response of Helicobacter pylori in broth was determined in the presence of ferrous sulfate, sodium pyruvate, and mucin (porcine stomach). The addition of either ferrous sulfate and sodium pyruvate or mucin to brain heart infusion broth with 7% horse serum (BHI-HS) enhanced the growth of H. pylori. The best growth of strain NB2-1, which was the slowest growing of 10 H. pylori strains evaluated, occurred in the presence of 0.05% ferrous sulfate and 0.05% sodium pyruvate. The addition of 0.3% mucin to BHI-HS reduced the lag time of H. pylori by 48 h and enhanced the growth. On the basis of the results for 10 H. pylori strains, the combination of ferrous sulfate (0.025%), sodium pyruvate (0.025%), and mucin (0.15%) in BHI-HS counteracted the inhibitory effects of the antibiotics used in culture media for selective growth of H. pylori. Results suggest that these supplements may be useful for enhancement of the growth of H. pylori in enrichment media.

scholarly journals Incidence and Biology of Corynespora cassiicola (Berk. & Curt.) Wei. disease of okra in Bangladesh

2014 ◽  
Vol 42 (2) ◽  
pp. 265-272 ◽  
Author(s):  
Fakhruddin Ali Ahmed ◽  
Nazmul Alam ◽  
Abul Khair
Keyword(s):  
Culture Media ◽  
Agar Medium ◽  
Potato Dextrose Agar ◽  
Corynespora Cassiicola ◽  
Optimum Growth ◽  
Potato Dextrose Agar Medium ◽  
Significant Incidence ◽  
Effects Of Temperature ◽  
A Minor

Eight commercial okra cultivars were evaluated to determine the magnitude of incidence of Corynespora cassiicola (Berk. & Curt.) Wei. Maximum and significant incidence of C. cassiicola was 14.86 in the cultivar BARI 1. In rest of the cultivars, the occurrence of this fungus was very poor to nil. Corynespora cassiicola appeared to be a minor pathogen for okra. Effects of temperature, pH and culture media on growth and sporulation of the fungus were investigated. Potato dextrose agar medium was found to be the most suitable for optimum growth and sporulation of this fungus at pH 7 and 25ºC. The conidial length significantly varied with the changes of pH. The correlation between pH and conidial length was found negative and highly significant. DOI: http://dx.doi.org/10.3329/bjb.v42i2.18028 Bangladesh J. Bot. 42(2): 265-272, 2013 (December)

scholarly journals Morphological Characterization of Corynespora cassiicola Isolates in Culture Media

2021 ◽  
Vol 13 (11) ◽  
pp. 74
Author(s):  
Jacqueline Dalbelo Puia ◽  
Adriano Thibes Hoshino ◽  
Estela Mariani Klein ◽  
Emily Danila De Almeida ◽  
Sandra Cristina Vigo ◽  
...  
Keyword(s):  
Growth Rate ◽  
Culture Medium ◽  
Culture Media ◽  
Agar Medium ◽  
Morphological Characterization ◽  
Corynespora Cassiicola ◽  
Cotton Production ◽  
Significance Level ◽  
Morphological Aspects ◽  
Host Origin

Corynespora cassiicola threatens soybean and cotton production in Brazil. The objective of this study was to evaluate cultural and morphological aspects of C. cassiicola isolated from soybean and cotton of different Brazilian regions, in culture media. The isolates were grown in PDA (Potato Dextrose Agar) and V8 juice agar media. The characteristics evaluated were: color, aspect, and growth rate of mycelia, as well as production and dimension of conidia, and number of septa per conidium. Culture media and isolates were compared using the Kruskal-Wallis or Tukey&rsquo;s test at 5% significance level. The mycelia of the isolates were predominantly dark gray and light brown. C. cassiicola isolates grew better in V8 juice agar medium, presenting a higher mycelial growth rate. In PDA medium, the production of conidia was higher in isolates from cotton, compared with soybean isolates. There was great variation in the production of conidia in V8 juice agar medium, regardless of the host origin. Conidia length and width varied for isolate and culture medium. The isolates of C. cassiicola coming from cotton presented a higher number of septa per conidium when grown in PDA medium. The morphological aspects of C. cassiicola vary depending on the host of origin and the culture medium.

scholarly journals Effect of Temeperature and PH on Growth of Alternaria tagetica, Leaf Blight of Marigold

2021 ◽  
Author(s):  
Karuna Chandrakant Kurhade ◽  
Hanumant Dnyandeo Gangawane ◽  
C.D. Deokar ◽  
K.S. Raghuwanshi
Keyword(s):  
Fungal Pathogen ◽  
Agar Medium ◽  
Severe Form ◽  
Leaf Blight ◽  
Potato Dextrose Agar ◽  
Effect Of Temperature ◽  
Common Disease ◽  
Optimum Temperature ◽  
Colony Diameter ◽  
Complete Failure

Marigold (Tagets erecta) is one of the most important major floriculture crop in India. The leaf blight of marigold is caused by Alternaria tagetica is becoming a common disease on marigold Alternaria blight appeared in mild to severe form in many regions of Maharashtra and sometimes resulting in complete failure of the crop especially during rainy season. The fungal pathogen are greatly influenced by environmental factor therefore the present work undertaken to study the effect of temperature and pH on growth of Alternaria tagetica. The effect of temperature and pH were determined by colony diameter method by using different media like potato dextrose agar and oat meal agar medium. It is clearly evident from the result that all the temperature pH tested showed variation in the diameter of colony. The temperature 25oC encouraged better growth of Alternaria tagetica as compared to 15oC and 35oC. The result also revealed that pH 6 and 7 encouraged better growth of Alternaria tagetica it is concluded from the result that temperature 25oC and pH 6 is optimum temperature and pH for the growth Alternaria tagetica.

Effect of Temperature on Effluent Quality in a Biological Wastewater Treatment Process

2019 ◽  
Vol 15 (1) ◽  
Author(s):  
E. Tejaswini ◽  
G. Uday Bhaskar Babu ◽  
A. Seshagiri Rao
Keyword(s):  
Growth Rate ◽  
Activated Sludge ◽  
Decay Rate ◽  
Kinetic Parameters ◽  
Temperature Coefficient ◽  
Oxygen Demand ◽  
Effect Of Temperature ◽  
Activated Sludge Process ◽  
Temperature Range ◽  
Wide Range

Abstract In the present study, the temperature effects on the organic carbon and nitrogen removal in an activated sludge process are evaluated. Benchmark Simulation Model No.1 (BSM1) based on activated sludge process is used for all the simulation purposes. A steady state simulation is performed to analyze the effluent concentrations with varying kinetic parameters obtained from different temperature coefficients over a wide range of temperatures from 15 °C to 35 °C. The temperature coefficient ‘a’ is assumed to have different set of values specific to the kinetic parameters, namely, Maximum heterotrophic growth rate \left( {{\mu _{mH}}} \right), Maximum autotrophic growth rate \left( {{\mu _{mA}}} \right), Heterotrophic decay rate \left( {{b_H}} \right), Autotrophic decay rate \left( {{b_A}} \right). The effluent concentration defined in terms of Chemical Oxygen Demand (COD), Biochemical Oxygen Demand (BOD), Total Suspended Solids (TSS), Total Nitrogen (TN) and Ammonia are observed to be significantly changing with a change in the kinetic parameters which are in turn a strong function of temperature coefficient. Emphasis is laid on the temperature range of 25–30 °C as it is commonly the most operated temperature range in a WWTP in India. It is also noticed that at temperatures <20 °C and >30 °C, the effluent limitations are violated from the standard values.

TEMPERATURE AND CULTURAL STUDIES ON UROCYSTIS TRITICI KOERN.

1949 ◽  
Vol 27c (3) ◽  
pp. 66-72 ◽  
Author(s):  
Yu-San Wu
Keyword(s):  
Growth Rate ◽  
Cultural Studies ◽  
Plant Tissue ◽  
Synthetic Medium ◽  
Wheat Seedling ◽  
Potato Dextrose Agar ◽  
Effect Of Temperature ◽  
Growth Studies ◽  
Petri Dishes ◽  
Geographical Isolates

A study of the effect of temperature on the infection of wheat by Urocystis tritici was made using Petri dishes in incubators held at various temperatures from 9° to 26 °C. The seedlings were transferred to pots as soon as the plumules broke through the coleoptiles. The percentages of infection obtained at various temperatures were as follows:—10.8% at 9° to 14 °C., 13.8 at 16°, 21.1 at 18°, 26.1 at 20°, 15.7 at 22°, 6.9 at 24°, and 3.0% at 26 °C. Growth studies of three geographical isolates of U. tritici were made on 12 different artificial media. Cultures were obtained by plating bits of disinfected unbroken, fresh, smutted plant tissue. The smut organism was found to be culturable on various media, though its growth rate was rather slow. The three isolates grew well on potato dextrose agar, Thaxter's hard agar, 4% sucrose potato agar, wheat seedling agar, and Czapek's synthetic medium, and were found to differ characteristically on five different media.

scholarly journals Morphological and cultural studies of Septoria vignicola

2014 ◽  
Vol 19 (1) ◽  
pp. 83-89
Author(s):  
R. D. Rawal ◽  
H. S. Sohi
Keyword(s):  
Mycelial Growth ◽  
Culture Media ◽  
Agar Medium ◽  
Carbon Sources ◽  
Potassium Nitrate ◽  
Potato Dextrose Agar ◽  
Optimum Growth ◽  
Carbon Supply ◽  
Wide Range ◽  
Agar Media

Pycnidial formation starts six days after inoculation. These arę globose, dark and range between 59.28-109.20 μm in diameier. The pycnidial wall is composed of 2- 3 layers of brown cells. The pycnidiospores ooze 8 days after inoculation. They are filiform, straight to curved, hyaline, septate with 2-4 septa, ends pointed or round and 21.84-68.64 x 1.25-2.15 μm. Mycelial growth was most profuse on Czapek Dox's agar medium, whereas maximum sporulation took place on Coon's, Sabauraud's and Potato dextrose agar media. The fungus could grow over a wide range of temperature from 12- 36°C (optimum: 24 ± l°C) and showed no growth at 40°C or above. Pycnidial formation occurred at 16- 28°C with a maximum at 24 ± l°C. Out of seven carbon sources tested, sorbose was the best for growth and sporulation of <i>Septoria vignicola</i> whereas maltose was a poor source for sporulation. The fungus responded very well to the supply of carbon in culture media as no growth of the fungus could be observed when the carbon supply was with held. Aspartic acid supported optimum growth among all the organic sources tried, whereas among inorganic sources potassium nitrate was the best, last growth observed on ammonium nitrate.

scholarly journals Comparison of Mycelial Growth of Different Tricholoma matsutake Strains in Soil Medium at Varying Temperatures

2019 ◽  
pp. 1-8 ◽  
Author(s):  
Maki Narimatsu ◽  
Muneyoshi Yamaguchi ◽  
Takashi Yamanaka ◽  
Seiki Gisusi ◽  
Tomonori Azuma ◽  
...  
Keyword(s):  
Growth Rate ◽  
Mycelial Growth ◽  
Linear Growth ◽  
Agar Medium ◽  
Effect Of Temperature ◽  
Tricholoma Matsutake ◽  
Mycelial Biomass ◽  
Soil Medium ◽  
Strain Collection ◽  
The Mean

Aims: To investigate the effect of temperature on the mycelial growth of the highly prized mushroom, Tricholoma matsutake, in soil medium. Methodology: Seven strains of T. matsutake collected from different areas of Japan were incubated in soil medium at 5, 10, 15, 20, 25, and 30°C for 89 day. After incubation, the linear growth of mycelia from the inoculum was measured on vertical lines under a dissecting microscope and classified by the cultivation day number to obtain the growth rate in each vial. The mycelial densities in the soil medium in all the vials were analyzed using qPCR to determine the mycelial biomass. Results: The mycelial growth of T. matsutake strains was mainly observed at 5–25°C. The optimum temperatures for linear mycelial growth and for the increase in mycelial density were 19.6°C and 17.6°C, respectively. These values were lower than those reported previously for T. matsutake grown on agar medium or in liquid medium. These optimum temperatures showed no clear relationship with the mean air temperature at the site of strain collection. Conclusion: It can be suggested that a temperature around 18°C is suitable for cultivation of T. matsutake mycelia in the soil medium.

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