scholarly journals Microsporogenesis in Brachiaria bovonei (Chiov.) Robyns and B. subulifolia (Mez) Clayton (Poaceae)

2009 ◽  
Vol 66 (5) ◽  
pp. 691-696 ◽  
Author(s):  
Claudicéia Risso-Pascotto ◽  
Maria Suely Pagliarini ◽  
Cacilda Borges do Valle
Keyword(s):  
Chromosome Number ◽  
High Frequency ◽  
Germplasm Collection ◽  
Meiotic Abnormalities ◽  
Meiotic Behavior ◽  
African Species ◽  
Germplasm Collections ◽  
Chromosome Stickiness ◽  
The Tropics ◽  
New Cultivars

Some African species of Brachiaria have been introduced into the Americas and became the most important forage for pastures in the tropics. New cultivars can be obtained either from direct selections from the natural existing variability in the germplasm collections or from interspecific hybridizations. Polyploidy is predominant in the genus Brachiaria and correlated with apomixis which complicates hybridization. The objective of cytological studies underway on the Brachiaria germplasm collection at Embrapa Beef Cattle is to determine the chromosome number and meiotic behavior of accessions. For the breeding of Brachiaria species, compatible sexual and apomictic accessions need to be identified. Microsporogenesis was evaluated in two accessions of Brachiaria bovonei (BO01 and BO05) and one accession of B. subulifolia (SU01). BO01 is pentaploid (2n = 5x = 45), BO05 is tetraploid (2n = 4x = 36), and SU01 is hexaploid (2n = 6x = 54), derived from x = 9. Meiotic abnormalities typical of polyploids, characterized by precocious chromosome migration to the poles in metaphases, laggard chromosomes in anaphases, and micronuclei in telophases and tetrads, were recorded in high frequency in all the accessions generating unbalanced gametes. Both accessions of B. bovonei presented chromosome stickiness. The results are discussed in the view of the Brachiaria breeding program objectives.

Meiotic Behavior in an Amphidiploid Rose and Its Hybrid Progeny

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 483d-483
Author(s):  
Yan Ma ◽  
David H. Byrne
Keyword(s):  
Meiotic Chromosome ◽  
Chromosome Association ◽  
Hybrid Progeny ◽  
Meiotic Abnormalities ◽  
Meiotic Behavior ◽  
Pollen Stainability ◽  
Meiotic Configuration ◽  
Genomic Relationships ◽  
Breeding Selection ◽  
Chromosome Stickiness

The amphidiploid rose 86-7 (Rosa wichuraiana × R. rugosa rubra) and its hybrids with the tetraploid breeding selection 82-1134 were analyzed for meiotic configuration frequencies and meiotic abnormalities. Genomic relationships in these hybrids were interpreted with the aid of a model of meiotic chromosome association in tetraploids. The optimized solutions to this model indicated a pattern intermediate between the “211” pattern, with one pair of closest genomes, and the “ring4” pattern, in which one of three possible pairing arrangements is strongly suppressed. The same configuration frequencies could also reflect a “4:0” pattern of equally similar genomes with more than two independent pairing and chiasma-forming domains per chromosome. The observed meiotic abnormalities included chromosome stickiness and asynchronous chromosome contraction within cells. Pollen stainability varied independently of meiotic irregularity or multivalent frequency.

scholarly journals Meiotic Studies in Some Species of Tribe Cichorieae (Asteraceae) from Western Himalayas

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Raghbir Chand Gupta ◽  
Henna Goyal ◽  
Vijay Singh ◽  
Rajesh Kumar Goel
Keyword(s):  
Chromosome Number ◽  
Pollen Viability ◽  
Intraspecific Variability ◽  
Pollen Grains ◽  
Early Prophase ◽  
Western Himalayas ◽  
Meiotic Abnormalities ◽  
Unreduced Pollen ◽  
Chromosome Stickiness ◽  
First Time

The present paper deals with meiotic studies in 15 species belonging to 6 genera of the tribe Cichorieae from various localities of Western Himalayas. The chromosome number has been reported for the first time inHieracium crocatum(2n=10) andLactuca lessertiana(2n=2x=16). Further, intraspecific variability has been reported for the first time inH. umbellatum(2n=2x=10and2n=6x=54),Tragopogon dubius(2n=2x=14and2n=4x=28), andT. gracilis(2n=2x=14). The chromosome report of2n=2x=10inYoungia tenuifoliais made for the first time in India. Maximum numbers of the populations show laggards, chromosome stickiness, and cytomixis from early prophase to telophase-II, leading to the formation of aneuploid cells or meiocytes with double chromosome number. Such meiotic abnormalities produce unreduced pollen grains and the reduced pollen viability.

scholarly journals Meiotic behavior during microsporogenesis of Alchornea triplinervia (Sprengel) Müller Argoviensis

2012 ◽  
Vol 42 (6) ◽  
pp. 1027-1032 ◽  
Author(s):  
Sara Mataroli de Godoy ◽  
Andréia Rodrigues Alonso Pereira ◽  
Mariza Barion Romagnolo ◽  
Claudicéia Risso-Pascotto
Keyword(s):  
Chromosome Number ◽  
Chromosome Segregation ◽  
Low Frequency ◽  
Optical Microscope ◽  
Pollen Grain ◽  
Meiotic Abnormalities ◽  
Meiotic Behavior ◽  
Segregation Process ◽  
Grain Formation ◽  
Brazilian Flora

The Alchornea triplinervia specie belongs to the Euphorbiaceae family, one of the main families of the Brazilian flora. In order to contribute to a better understanding of the specie, a counting of chromosome number and the microsporogenesis analysis of A. triplinervia were done. The inflorescences were collected in the municipalities of Paranavaí and Diamante do Norte, State of Paraná, Brazil, and the slides were prepared by squashing technique and staining with 1% acetic carmine. The analysis were performed using an optical microscope and showed a chromosome number for the specie equal to 2n=8x=72. Irregularities in the chromosome segregation process were the main meiotic abnormalities, presenting typical polyploid behavior. Other irregularities were observed; however, at low frequency without compromising the pollen grain formation of the analyzed plants.

scholarly journals Chance or Necessity—The Fungi Co−Occurring with Formica polyctena Ants

Insects ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 204
Author(s):  
Igor Siedlecki ◽  
Michał Gorczak ◽  
Alicja Okrasińska ◽  
Marta Wrzosek
Keyword(s):  
Molecular Identification ◽  
High Frequency ◽  
Its Rdna ◽  
The Other ◽  
Formica Polyctena ◽  
Ant Nests ◽  
Mutualistic Fungi ◽  
The Tropics ◽  
High Diversity ◽  
Better Than

Studies on carton nesting ants and domatia−dwelling ants have shown that ant–fungi interactions may be much more common and widespread than previously thought. Until now, studies focused predominantly on parasitic and mutualistic fungi–ant interactions occurring mostly in the tropics, neglecting less−obvious interactions involving the fungi common in ants’ surroundings in temperate climates. In our study, we characterized the mycobiota of the surroundings of Formica polyctena ants by identifying nearly 600 fungal colonies that were isolated externally from the bodies of F. polyctena workers. The ants were collected from mounds found in northern and central Poland. Isolated fungi were assigned to 20 genera via molecular identification (ITS rDNA barcoding). Among these, Penicillium strains were the most frequent, belonging to eight different taxonomic sections. Other common and widespread members of Eurotiales, such as Aspergillus spp., were isolated very rarely. In our study, we managed to characterize the genera of fungi commonly present on F. polyctena workers. Our results suggest that Penicillium, Trichoderma, Mucor, Schwanniomyces and Entomortierella are commonly present in F. polyctena surroundings. Additionally, the high diversity and high frequency of Penicillium colonies isolated from ants in this study suggest that representatives of this genus may be adapted to survive in ant nests environment better than the other fungal groups, or that they are preferentially sustained by the insects in nests.

Cytogenetic studies of leafy spurge, Euphorbia esula, and its allies (Euphorbiaceae)

1988 ◽  
Vol 66 (11) ◽  
pp. 2247-2257 ◽  
Author(s):  
A. E. Stahevitch ◽  
C. W. Crompton ◽  
W. A. Wojtas
Keyword(s):  
Seed Germination ◽  
Chromosome Number ◽  
Natural Populations ◽  
Leafy Spurge ◽  
Euphorbia Esula ◽  
Meiotic Abnormalities ◽  
Stability Studies ◽  
Polymorphic Species ◽  
Cytogenetic Studies ◽  
Euphorbia Cyparissias

Cytological and cytogenetic studies were carried out on populations of leafy spurge (Euphorbia esula L. s.l.) and its allies primarily from North America but also from Europe. Chromosome numbers were determined for 126 samples of E. esula, 11 of E. cyparissias L., 1 of the hybrid (E. ×pseudoesula Schur) between these two species, and 1 of E. agraria Bieb. All plants of E. esula were hexaploid. Of the total, 125 leafy spurge accessions had a chromosome number of n = 30; 1 of n = 25++. Very few meiotic abnormalities were observed. Euphorbia cyparissias was primarily tetraploid (n = 20), although occasional diploids (n = 10) were encountered. Tetraploids were fertile; diploids were sterile. The hybrid between the two foregoing species had a chromosome number of n = 25, indicating that the E. cyparissias parent was a tetraploid; meiosis in the hybrid was abnormal. Euphorbia agraria was found to have a gametic number of n = 20, which is the first chromosome number determination for this species; meiosis was normal. Artificial crosses were made successfully between 31 accessions of leafy spurge. Seed germination of the F1 progeny slightly exceeded that reported for natural populations, and meiosis was normal. Pollen stability studies were carried out on herbarium material. Stainability was 100% for most of the samples studied. Extensive pollen size polymorphism was found. It is suggested that this phenomenon supports the hypothesis that E. esula is of allopolyploid origin. No cytological or cytogenetic basis was found for considering the leafy spurge accessions examined in this study as other than as a single, albeit somewhat polymorphic, species.

Meiotic chromosome behaviour in Cenchrus ciliaris

Bothalia ◽  
1998 ◽  
Vol 28 (1) ◽  
pp. 83-90 ◽  
Author(s):  
N. C. Visser ◽  
J. J. Spies
Keyword(s):  
Chromosome Number ◽  
Meiotic Chromosome ◽  
Basic Chromosome Number ◽  
Chromosome Behaviour ◽  
Meiotic Abnormalities ◽  
Single Specimen ◽  
Cenchrus Ciliaris ◽  
Basic Chromosome ◽  
Single Genome ◽  
Meiotic Irregularities

A basic chromosome number of x = 9 has been confirmed for Cenchrus ciliaris L. Polyploidy is common and levels vary from tetraploid to hexaploid. Aneuploidv is reported for a single specimen, where two chromosomes of a single genome were lost. Various meiotic irregularities were observed. The highest incidence of meiotic abnormalities was observed in the pentaploid specimens. This was attributed to their uneven polyploid level All specimens varied from segmental alloploid to alloploid.

Cytogenetic abnormalities of cotton somaclones from callus cultures

Genome ◽  
1989 ◽  
Vol 32 (5) ◽  
pp. 762-770 ◽  
Author(s):  
David M. Stelly ◽  
D. W. Altman ◽  
R. J. Kohel ◽  
T. S. Rangan ◽  
E. Commiskey
Keyword(s):  
Cell Culture ◽  
Tissue Culture ◽  
Somaclonal Variation ◽  
High Frequency ◽  
Chromosomal Abnormalities ◽  
Callus Cultures ◽  
Meiotic Abnormalities ◽  
High Frequencies ◽  
Field Environment ◽  
Metaphase I

Somaclonal variation occurs among regenerants from tissue culture of many plant species. Our objective was to determine whether cytogenetic variation contributes to somaclonal variation in cotton (Gossyptum hirsutum L.,2n = 4x = 52). Of 117 somaclones of cotton regenerated from 18-month-old callus cultures of 'SJ-2' and 'SJ-5' cultivars, 35 were analyzed for meiotic abnormalities. The population of somaclones was extremely varied in phenotype, most plants being strikingly aberrant in phenotype. Fertility was generally poor: 84% failed to set bolls and only 5% set 10 or more bolls in a field environment. Only one of the somaclones (3%) formed 26 bivalents at metaphase I. Fourteen were nonsynaptic to partially synaptic at metaphase I. Synaptic abnormalities impaired fertility and precluded thorough metaphase analysis. Chromosome numbers obtained for 32 plants ranged from 49 to 53, and only 1 plant was hyperaneuploid. No plant was polyploid. Chromosomal abnormalities in plants with normal metaphase pairing included univalents, unequal bivalents, rod bivalents, trivalents, open quadrivalents, and centric fragments. Seventeen hypoaneuploid plants formed a V-shaped trivalent at metaphase I, constituting a high frequency of tertiary monosomy. The high frequencies of aneuploidy and tertiary monosomy indicate that cytogenetic anomalies are a major source of somaclonal variation in cotton. It is hypothesized that (i) primary cytogenetic events during cotton cell culture give rise to breakage – fusion – bridge (BFB) cycles, (ii) BFB cycles accrue during culture, (iii) BFB cycles cause loss of chromatin, and (iv) BFB cycles are resolved by the formation of stable tertiary chromosomes with mono-centric activity. The hypothesis accounts mechanistically for the coincidence of chromatin deficiencies and chromatin exchange involved implicitly in tertiary monosomy, as well as for the relatively high frequency of tertiary monosomy among somaclones.Key words: aneuploid, monosomic, synaptic, sterility, Gossypium.

scholarly journals Global Wavenumber Spectrum with Corrections for Altimeter High-Frequency Noise

2015 ◽  
Vol 45 (2) ◽  
pp. 495-503 ◽  
Author(s):  
Xiao-Hui Zhou ◽  
Dong-Ping Wang ◽  
Dake Chen
Keyword(s):  
High Frequency ◽  
Acoustic Doppler Current Profiler ◽  
High Energy ◽  
Frequency Noise ◽  
Spectral Estimates ◽  
Current Profiler ◽  
Adcp Measurements ◽  
Corrected Data ◽  
Geostrophic Turbulence ◽  
The Tropics

AbstractThe altimetry wavenumber spectra of sea surface height (SSH) provide a unique dataset for testing of geostrophic turbulence. While SSH spectral slopes of k−11/3 and k−5 are expected from theories and numerical simulations, the altimetry spectra from the original unfiltered and instrument noise–corrected data often are too shallow, falling between k−2 and k−3. In this study, the possibility that the flattened spectral slopes are partly due to contamination by unresolved high-frequency (<10 days) motions is tested. A spatiotemporal filter based on empirical orthogonal function expansion (EOF) is used to remove the temporally incoherent signals. The resulting spectral slopes are much steeper than in the previous studies. Over 70% of the revised global spectral estimates, excluding the tropics, are above k−3. Moreover, in high energy regions like the Gulf Stream and Kuroshio, the spectral slopes are about k−5, which is consistent with the classical quasigeostrophic (QG) turbulence. The spectral slopes are validated with the eddy kinetic energy (EKE) spectra from shipboard acoustic Doppler current profiler (ADCP) measurements in the high and low energy regions.

scholarly journals Interspecific Genome Size and Chromosome Number Variation Shed New Light on Species Classification and Evolution in Caladium

2014 ◽  
Vol 139 (4) ◽  
pp. 449-459 ◽  
Author(s):  
Zhe Cao ◽  
Zhanao Deng ◽  
Mike Mclaughlin
Keyword(s):  
Chromosome Number ◽  
Genome Size ◽  
Somatic Chromosome ◽  
Genome Duplication ◽  
Germplasm Collection ◽  
Size Group ◽  
Root Tip ◽  
Species Status ◽  
Species Classification ◽  
Duplication Events

The genus Caladium Vent. is a member of the family Araceae; some of its species are cultivated as ornamentals. The present study was conducted to determine the genome size, somatic chromosome number, and their variation within 63 accessions representing 10 species of Caladium. Caladium genome sizes estimated using propidium iodide staining and flow cytometry ranged from 2.98 pg/2C in Caladium lindenii Engl. to 9.89 pg/2C in Caladium ×hortulanum Birdsey ‘Chang Suek’. Two genome size groups (large and small) were evident among the 63 caladium accessions. The average genome size of 36 caladium accessions in the large genome size group was 9.29 pg/2C, roughly twice that of the 27 accessions in the small genome size group (4.50 pg/2C). Microscopic examination of squashed root tip cells revealed seven somatic chromosome numbers among 39 caladium accessions, including 2n = 18, 20, 24, 26, 30, 34, and 38, and provided the first chromosome counts for four caladium species new to Caladium. The results support the species status of C. marmoratum Mathieu ex K. Koch, C. picturatum K. Koch & C.D. Bouché, and C. steudneriifolium Engl. that were merged into C. bicolor (Aiton) Vent. previously and also support the species status of C. clavatum Hett., Bogner & J. Boos, and C. praetermissum Bogner & Hett., two species recently established in or transferred to Caladium. The results suggest that C. bicolor and C. schomburgkii Schott, not C. picturatum or C. marmoratum, are the chief parents of the fancy-leaved caladium (C. ×hortulanum). Four caladium cytotype groups (CCG-1 to -4) were identified in scatterplot of chromosome number vs. genome size. The genome size of C. bicolor, C. schomburgkii, and C. ×hortulanum in the CCG-4 is approximately twice that of C. humboldtii (Raf.) Schott and C. picturatum in the CCG-2, and the chromosome number of C. clavatum and C. marmoratum in the CCG-3 is close to twice that of C. humboldtii and C. picturatum in the CCG-2, both suggesting possible genome duplication or tetraploidization events in Caladium. However, the chromosome number of the CCG-4 species does not correspond to an expected 2n = 36 or 40, and the genome size of the CCG-3 species does not correspond to an expected 8.98 pg/2C. Conflicts between genome size and chromosome number indicate that genome duplication events were likely followed by chromosome fusions/losses in the formation of CCG-4 species and DNA losses likely followed tetraploidization in the formation of the CCG-3 species. The high level of cytological diversity found within Caladium affects germplasm collection and preservation efforts as well as breeding programs in the genus.

scholarly journals Chromosome Number and Meiotic Behavior of Cultivated Chia, Salvia hispanica (Lamiaceae)

HortScience ◽  
1990 ◽  
Vol 25 (12) ◽  
pp. 1646-1647 ◽  
Author(s):  
A. Estilai ◽  
A. Hashemi ◽  
K. Truman
Keyword(s):  
Chromosome Number ◽  
Species Identification ◽  
Southern California ◽  
Herbarium Specimens ◽  
Meiotic Behavior ◽  
Root Tips ◽  
Chromosome Counts ◽  
Salvia Hispanica ◽  
Meiotic Analyses ◽  
Metaphase I

Edible chia seeds, purchased from the local markets in Guatemala, Mexico, and southern California, were used for species identification, chromosome counts, karyotype construction, and meiotic analyses. Plants raised from those seeds had ovate leaves, dense racemose inflorescences, pale-blue flowers, and were identified from herbarium specimens as Salvia hispanica L. Mitotic analyses of root tips from 50 plants showed 2n = 12—the lowest chromosome number in the genus. Chromosomes were small, ranging from 2 to 3.5 pm. One pair of chromosomes was metacentric (with the long arm: short arm ratio, r = 1.5), four pairs were submetacentric (r = 2.6 to 3.6), and one pair was telocentric (r = 12). Meiosis was regular and six bivalents were observed at metaphase I. Ring and rod bivalents averaged 1.53 ± 1.05 and 4.47 ± 1.05, respectively;

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