scholarly journals Composition and evaluation of the antimicrobial activity of the essential oil of Senecio selloi Spreng DC.

2013 ◽  
Vol 15 (4) ◽  
pp. 503-507 ◽  
Author(s):  
G.N.S. Silva ◽  
T.B. Spader ◽  
S.H. Alves ◽  
C.A. Mallmann ◽  
B.M. Heinzmann

The essential oil of the aerial parts of Senecio selloi Spreng. DC. was extracted by hydrodistillation and analyzed by GC/MS. Nineteen compounds were identified, representing 99.9% of the total. The main compounds were found to be sesquiterpene hydrocarbons (71.3%), most of them with a bisabolane skeleton (59.4%). The major constituent was α-zingiberene (54%), followed by monoterpene α-isolimonene (16%). The essential oil was also tested against two Gram-positive and two Gram-negative bacterial species, three yeasts, and an algae. From the strains assayed, only Bacillus subtilis ATCC 6633 showed susceptibility (MIC and MBC = 4400 µg/mL) to the essential oil.

2004 ◽  
Vol 59 (9-10) ◽  
pp. 653-656 ◽  
Author(s):  
Ali Sonboli ◽  
Peyman Salehi ◽  
Morteza Yousefzadi

Abstract The composition and antimicrobial activity of the essential oil of Nepeta crispa Willd., an endemic species from Iran, was studied. The oil was obtained from the aerial parts of the plant and analyzed by GC and GC/MS. Twenty-three compounds, accounting for 99.8% of the total oil, were identified. The main constituents were 1,8-cineol (47.9%) and 4aα,7α,7aβ- nepetalactone (20.3%). The antimicrobial activity of essential oil of N. crispa was tested against seven gram-negative or gram-positive bacteria and four fungi. The results of the bioassays showed the interesting antimicrobial activity, in which the gram-positive bacteria, Bacillus subtilis and Staphylococcus aureus, were the most sensitive to the oil. Also, the oil exhibited a remarkable antifungal activity against all the tested fungi.


2007 ◽  
Vol 2 (12) ◽  
pp. 1934578X0700201 ◽  
Author(s):  
Ali Sonboli ◽  
Mohammad Reza Kanani ◽  
Morteza Yousefzadi ◽  
Mehran Mojarrad

The aerial parts of Tetrataenium nephrophyllum were collected at the flowering stage, hydrodistilled, and the essential oil was analyzed by GC and GC-MS. Forty components accounting for 97.9% of the total oil were identified. Germacrene D (38.5%), 2-ethylhexyl acetate (11.2%), n-octyl 2-methylbutanoate (9.2%) and geranyl isovalerate (8.3%) were the major constituents. Sesquiterpene hydrocarbons (51.3%) and aliphatic esters (40.4%) were found to be the main group of compounds. The antimicrobial activity of the essential oil of T. nephrophyllum was determined against seven Gram-positive and Gram-negative bacteria (Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus, S. epidermidis, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae), as well as three fungi (Candida albicans, Saccharomyces cerevisiae and Aspergillus niger). The bioassay showed that the oil exhibited moderate to high antimicrobial activity.


2017 ◽  
Vol 19 (2) ◽  
pp. 183 ◽  
Author(s):  
F. Sadyrbayeva ◽  
G. Akhmetova ◽  
K. Praliyev ◽  
H. Osman ◽  
N. Korotetskaya

1-alkyl-4-(3-naphthyloxyprop-1-ynyl)piperidine-4-ols have been obtained by the condensation of 1-alkyl-pipieridin-4-ones with 1- and 2-naphthoxypropines by the Favorsky method in absolute diethylether, in the presence of powered technical KОН, under atmospheric pressure. Acylation of tertiary naphthoxypropynyl piperidols by cyclopropanecarbonylchloride has been carried out in order to introduce a cyclopropanecarbonyl fragment into the structures of naphthoxypropargylpiperidines. The obtained esters of cyclopropanecarboxylic acid represent crystalline substances with the definite melting temperature, very soluble in water, ethanol, acetone. The composition and structure of the synthesized compounds have been confirmed by the data of elemental analysis, IR-spectroscopy, NMR-spectroscopy, the identity has been confirmed by thin-layer chromatography. The compounds under the code AIP (anti-infective preparation) have been studied for an antimicrobial activity in relation to museum microbial strains. The effects of these preparations have been assessed in vitro in relation to Bacillus subtilis ATCC 6633, Escherichia coli ATCC 25922, Salmonella enterica ATCC 14028 and Staphylococcus aureus ATCC 6538- P. It has been found that the compounds AIP-30 and AIP-31 possess an antimicrobial activity in relation to all strains of microorganisms, engaged in the experiment. AIP- 30 and AIP-31 have antimicrobial effects to different extents, AIP-30 has displayed the highest activity in relation to the museum strain Bacillus subtilis ATCC 6633 in the concentration of 250 μg/ml. It has been established that AIP-32 displays a selective antimicrobial activity towards one type, and AIP-33 ‒ towards two types of the museum strains, engaged in the experiment.


2021 ◽  
Vol 11 (1) ◽  
pp. 108-109
Author(s):  
Kinjal H Shah ◽  
Piyush M. Patel

Evaluation of antimicrobial activity was performed by cup-plate method. The test microorganisms used for the antimicrobial activity were four bacterial species (two Gram positive and two Gram negative) – Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeuroginosa,Escherichia coli. The test microorganisms used for the antimicrobial activity were four bacterial species (two Gram +ve and two Gram -ve) Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeuroginosa,Escherichia coli.  


2009 ◽  
Vol 4 (4) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Daniele Fraternale ◽  
Anahi Bucchini ◽  
Laura Giamperi ◽  
Donata Ricci

The chemical composition of the essential oil of Ballota nigra L. ssp foetida obtained from the flowering aerial parts was analyzed by GC/MS. From the 37 identified constituents of the oil, β-caryophyllene (20.0%), germacrene D (18.0%) and caryophyllene oxide (15.0%) were the major components. The oil was active against both Gram-negative and Gram-positive bacteria as well as against three Candida species.


2020 ◽  
Vol 14 (1) ◽  
pp. 49-62
Author(s):  
Mauro Ezio Eustáquio Pires ◽  
Adriano Guimarães Parreira ◽  
Tuânia Natacha Lopes Silva ◽  
Heloísa Carneiro Colares ◽  
José Antonio da Silva ◽  
...  

Background: Numerous causes of infection in arthroplasties are related to biofilm formation on implant surfaces. In order to circumvent this problem, new alternatives to prevent bacterial adhesion biosurfactants-based are emerging due to low toxicity, biodegradability and antimicrobial activity of several biosurfactants. We revised all patents relating to biosurfactants of applicability in orthopedic implants. Methods: This work aims to evaluate the capability of a lipopeptide produced by Bacillus subtilis ATCC 19659 isolates acting as inhibitors of the adhesion of Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 29213 onto titanium and stainless steel surfaces and its antimicrobial activity. Results: The adhesion of the strains to the stainless-steel surface was higher than that of titanium. Preconditioning of titanium and stainless-steel surfaces with 10 mg mL-1 lipopeptide reduced the adhesion of E. coli by up to 93% and the adhesion of S. aureus by up to 99.9%, suggesting the strong potential of lipopeptides in the control of orthopedic infections. The minimal inhibitory concentration and minimum bactericidal concentration were 10 and 240 µg mL-1 for E. coli and S. aureus, respectively. Conclusion: The lipopeptide produced by Bacillus subtilis ATCC 19659 presented high biotechnological application in human health against orthopedic implants infections.


2016 ◽  
Vol 78 (6-8) ◽  
Author(s):  
Noor Izzatie Munira Kamaruddin ◽  
Nor Azah Mohamad Ali ◽  
Muhd Fauzi Safian ◽  
Zaidah Zainal Ariffin

Polyalthia stenopetala essential oils were extracted by hydrodistillation from the leaves and were analyzed using gas chromatography-flame ionization detector (GC-FID) and gas chromatography/mass spectrometry (GC/MS) system. Thirty one compounds were identified from the analysis. The most abundant components in the leaves oil are curzerene (37.56%) followed by viridiflorol (11.59%), germacrene B (3.77%) and aromadendrene (4.01%). The antimicrobial activity of the oil essential oils was determined with disk diffusion method and minimum inhibitory concentration (MIC) assay. Four bacteria, Staphylococcus aureus (ATCC 25923), Bacillus subtilis (ATCC 6633), Escherichia coli (ATCC 25922) Psedomonas aeruginosa (ATCC 10145) and two yeasts, Candida albicans (ATCC 10231) and Saccharomyces cerevisiae (ATCC 4098) were selected. The crude oil shows the most reactivity against B. subtilis (ATCC 6633) and C. albicans (ATCC 10231) with an inhibitory zone of 11mm. The minimum inhibitory concentration (MIC) of sample against Staphylococcus aureus (ATCC 25923), Bacillus subtilis (ATCC 6633), Escherichia coli (ATCC 25922) Psedomonas aeruginosa (ATCC 10145) and Candida albicans (ATCC 10231) in range of 0.5 mg/ml – 1.0 mg/ml which can categorized as strong.


2009 ◽  
Vol 4 (7) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Rajesh K. Joshi ◽  
Chitra Pande ◽  
Mohammad H. K. Mujawar ◽  
Sanjiva D. Kholkute

The essential oil composition of the aerial parts of Anaphalis nubigena DC. var. monocephala (DC.) C. B. Clarke collected from Pindari glacier at a height of 3300 m, was analyzed by using GC and GC/MS. Sixty components were identified, accounting for 95.9% of the total oil. The main constituents were α-guaiene (12.3%), γ-muurolene (10.4%), γ-cadinene (8.3%), α-muurolol (7.4%), α-gurjunene (6.0%) and α-bulnesene (5.8%). The oil was found to be rich in sesquiterpene hydrocarbons (60.1%). The oil was active against Escherichia coli (NCIM 2065) and Klebsiella pneumoniae (NCIM 2957), with MIC values of 125 μg/mL and 500 μg/mL, respectively.


2020 ◽  
Vol 10 ◽  
Author(s):  
Navadha Bhatt ◽  
Navabha Joshi ◽  
Kapil Ghai ◽  
Om Prakash

Background: The Lamiaceae (Labiatae) is one of the most diverse and widespread plant families’ in terms of ethno medicine and its medicinal value is based on the volatile oils concentration. This family is important for flavour, fragrance and medicinal properties. Manyplants belonging to this family have indigenous value. Method: The essential oil of Plectranthus gerardianusBenth. (Lamiaceae), was analysed by GC and GC-MS analysis, while the major component was isolated and conformed by NMR spectroscopy. Result: The oil was found to be rich in oxygenated monoterpenes, which contribute around 62% of the total oil. The major components identified were fenchone (22.90%) and carvenone oxide (16.75%), besides other mono and sesquiterpenoids. The in-vitro antimicrobial activity of essential oil was tested against three gram negative bacteria viz. Pasteurellamultocida, Escherichia coli, and Salmonella enterica, two gram positive bacteria viz. Staphylococcus aureus and Bacillus subtilis and two fungi viz. Candida albicans and Aspergillusflavus. The antimicrobial activity of the oil was also compared to the antimicrobial activity of leaf essential oil of another Himalayan plant viz. Nepetacoerulescens. Conclusion: The oil showed in-vitro antimicrobial activity against all the microbial strains and can lessen the ever-growing demand of potentially hazardous antibiotics for treatment.


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