scholarly journals New Anti-Infective Preparations of Naphthyloxypropargylpiperidine Series

2017 ◽  
Vol 19 (2) ◽  
pp. 183 ◽  
Author(s):  
F. Sadyrbayeva ◽  
G. Akhmetova ◽  
K. Praliyev ◽  
H. Osman ◽  
N. Korotetskaya
Keyword(s):  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Subtilis Atcc ◽  
Composition And Structure ◽  
Cyclopropanecarboxylic Acid ◽  
Bacillus Subtilis Atcc ◽  
Microbial Strains ◽  
Selective Antimicrobial Activity ◽  
Layer Chromatography

1-alkyl-4-(3-naphthyloxyprop-1-ynyl)piperidine-4-ols have been obtained by the condensation of 1-alkyl-pipieridin-4-ones with 1- and 2-naphthoxypropines by the Favorsky method in absolute diethylether, in the presence of powered technical KОН, under atmospheric pressure. Acylation of tertiary naphthoxypropynyl piperidols by cyclopropanecarbonylchloride has been carried out in order to introduce a cyclopropanecarbonyl fragment into the structures of naphthoxypropargylpiperidines. The obtained esters of cyclopropanecarboxylic acid represent crystalline substances with the definite melting temperature, very soluble in water, ethanol, acetone. The composition and structure of the synthesized compounds have been confirmed by the data of elemental analysis, IR-spectroscopy, NMR-spectroscopy, the identity has been confirmed by thin-layer chromatography. The compounds under the code AIP (anti-infective preparation) have been studied for an antimicrobial activity in relation to museum microbial strains. The effects of these preparations have been assessed in vitro in relation to Bacillus subtilis ATCC 6633, Escherichia coli ATCC 25922, Salmonella enterica ATCC 14028 and Staphylococcus aureus ATCC 6538- P. It has been found that the compounds AIP-30 and AIP-31 possess an antimicrobial activity in relation to all strains of microorganisms, engaged in the experiment. AIP- 30 and AIP-31 have antimicrobial effects to different extents, AIP-30 has displayed the highest activity in relation to the museum strain Bacillus subtilis ATCC 6633 in the concentration of 250 μg/ml. It has been established that AIP-32 displays a selective antimicrobial activity towards one type, and AIP-33 ‒ towards two types of the museum strains, engaged in the experiment.

scholarly journals Preliminary Characterization of the Probiotic Properties of a Bacterial Strain for Used in Monogastric Nutrition

2019 ◽  
Vol 76 (2) ◽  
pp. 102 ◽  
Author(s):  
Mihaela DUMITRU ◽  
Mihaela HĂBEANU ◽  
Cristina TABUC ◽  
Ștefana JURCOANE
Keyword(s):  
Bacillus Subtilis ◽  
Bile Salts ◽  
Good Growth ◽  
Probiotic Properties ◽  
Simulated Intestinal Fluid ◽  
Phenotypic Profile ◽  
Subtilis Atcc ◽  
Bacillus Subtilis Atcc

This study aimed to evaluate some probiotic properties of Bacillus subtilis ATCC 6051a. The phenotypic profile, resistance to pH by simulated gastric juice (pH 2 and 3), bile salts by simulated intestinal fluid, survivability (%), heat and antibiotics tolerance were investigated. The strain is a Gram-positive, rod-shaped bacteria, arranged in short chains or in small irregular pairs with the ability to produce spores. Good viability at pH 2 and 3, with a survival of more than ≥80%, was found. In the presence of bile salts 0.3%, over 4 h, the strain exhibited a survival ≥85%. At 80°C, for 120 min., the strain showed good growth (9.04 log CFU/ml). Results were sensitive to most antibiotics, with a highly susceptible (between 16 – 25 mm) to erythromycin, clindamycin, amoxicillin, chloramphenicol, ciprofloxacin, amikacin and kanamycin. The strain was found to be sensitive to vancomycin, gentamicin, and tetracycline. The present research demonstrated that Bacillus subtilis ATCC 6051a can survive under gastrointestinal conditions, which involves them to future in vitro and in vivo probiotic studies.

scholarly journals Rizobacterias con potencial antagonista in vitro a Mycosphaerella fijiensis Morelet

2020 ◽  
Vol 13 (2) ◽  
Author(s):  
Karen Tatiana Chávez Arteaga ◽  
Jefferson Javier Guato Molina ◽  
Jorge Luis Rodríguez Acosta ◽  
Ángel Virgilio Cedeño Moreira ◽  
Ricardo Fernando Romero Meza ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Pseudomonas Putida ◽  
Serratia Marcescens ◽  
Pseudomonas Fluorescens ◽  
Mycosphaerella Fijiensis ◽  
Subtilis Atcc ◽  
Bacillus Subtilis Atcc ◽  
Pseudomonas Veronii ◽  
Enterobacter Asburiae

El empleo de bio-controladores en la agricultura beneficia los aspectos fisiológicos en plantas, a diferencia de la constante aplicación de pesticidas en el cultivo del banano ha ocasionado la pérdida de la sensibilidad en M. fijiensis, reduciendo la microbiota del suelo. El objetivo se enfocó en caracterizar el potencial antagónico de las PGPR en inhibición de germinación de ascósporas y desarrollo micelial de M. fijiensis. Se realizaron cultivos monospóricos de M. fijiensis e identificado por PCR. Se evaluaron los extractos celulares de Pseudomonas putida PB3-6, Klebsiella variicola BO3-4, Enterobacter asburiae BA4-19, Serratia marcescens PM3-8, Enterobacter asburiae PM3-14, Pseudomonas protegens CHA0, Pseudomonas fluorescens WCS417, Pseudomonas veronii R4 y Bacillus subtilis ATCC 5540 para sus evaluaciones antagonistas: a) Inhibición del tubo germinativo de las ascósporas al 2% y b) Desarrollo micelial al (2 y 10 %). La PCR empleado en la identificación de M. fijiensis se confirma el producto de amplificación de 1018 pb. El factor antagónico de los extractos celulares al 2 % de PM3-14 y CHA0 inhibe sobre el 80 % al desarrollo de los tubos germinativos. La inhibición al desarrollo micelial del extracto celular al 2 %, de CHA0 logró una efectividad del 54 % y las cepas (PM3-8, PM3-14 y BA4-19) con (32, 26 y 26 %). Al 10 % del extracto de la cepa PM3-8 inhibe el desarrollo micelial con niveles de turbidez de 0,47 (OD600nm). El empleo de estos bio-controladores en la agricultura ofrecerá una alternativa para beneficiar en la reducción del uso de agroquímicos

scholarly journals Antibacterial Activity of Synthesized Derivatives of Purpurin Containing Cyсlopropane and Cyclobutane Fragment

2020 ◽  
Vol 22 (3) ◽  
pp. 213
Author(s):  
Т.V. Kharlamova ◽  
A.V. Gabdrakipov ◽  
P.B. Seidakhmetova ◽  
K.D. Praliyev
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Acid Chloride ◽  
Subtilis Atcc ◽  
Bacillus Subtilis Atcc ◽  
Cyclobutanecarboxylic Acid ◽  
Mic Value

The present study is a continuation of the scientific research works for the synthesis of anthraquinonе-containing derivatives with cyclic ring systems by the interaction of 1,2,4-trihydroxyanthraquinone (purpurin) with cyclopropanecarboxylic acid chloride and cyclobutanecarboxylic acid chloride by using the ultrasonic treatment. Esters of purpurin, studied for antibacterial activity in museum test strains of microorganisms (Staphylococcus aureus ATCC 6538-P, Bacillus subtilis ATCC 6633, Escherichia coli ATCC 25922, Pseudomonas aeruginosa АТСС 27853, Сandida albicans АТСС 10231) in vitro with the determination of the minimum inhibitory concentration (MIC) value. The study revealed that the cyclopropane substituent exhibits moderate antibacterial activity against Bacillus subtilis ATCC 6633. While with the cyclobutane moiety it had a weak effect with respect to Pseudomonas aeruginosa ATCC strain. It has been found that the compounds with the cyclopropane and cyclobutane fragment have possessed antimicrobial activity in relation to strains of microorganisms Staphylococcus aureus АТСС 6538 for which the MIC value was 62.5 μg/ml.

scholarly journals Actividad antibacteriana de extractos hexánico y etanólico de macroalgas marinas de la Bahía de Ancón, Lima – Perú

2020 ◽  
Vol 31 (2) ◽  
pp. e17829
Author(s):  
Ramón Muñoz A. ◽  
Selma Santome ◽  
Jorge León Q.
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Salmonella Typhimurium ◽  
San Francisco ◽  
Subtilis Atcc ◽  
Bacillus Subtilis Atcc ◽  
Para Determinar

En el presente trabajo se evaluaron las actividades antimicrobianas de extractos etanólicos y hexánicos de nueve especies de macroalgas marinas recolectadas en la zona intermareal de la playa San Francisco, Ancón, de Lima, Perú. La evaluación se realizó in vitro frente a un panel de bacterias estándar Gram positivas (Staphylococcus aureus ATCC 6538, Bacillus subtilis ATCC 6633 y Enterococcus faecalis ATCC 51922) y Gram negativas (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 9027 y Salmonella Typhimurium ATCC 14028). Los extractos etanólicos fueron de mayor rendimiento (67%) en comparación a los hexánicos (33%), en especial frente a los Gram positivos, siendo el extracto obtenido de Ulva enteromorpha var. intestinalis y U. nematoidea los más activos frente a S. aureus ATCC 6538 (74.1 y 78.2 % de inhibición, respectivamente). Los extractos etanólicos de Cladophora sp y U. nematoidea frente a B. subtilis ATCC 6633 presentaron inhibiciones entre moderada y baja (52.5 y 33%, respectivamente). Los extractos etanólicos de U. enteromorpha y Cladophora sp frente a S. Typhimurium presentaron solo 40% de inhibición. El extracto etanólico de Cladophora sp fue el único que presentó actividad antimicrobiana frente a las cinco cepas bacterianas y fue elegida para determinar la Concentración Mínima Inhibitoria (CMI), que resultó en 12.5 y 25 mg/ml frente a B. subtilis ATCC 6633 y S. aureus ATCC 6538, respectivamente. Se concluye que, la mayoría de las macroalgas estudiadas contienen compuestos inhibitorios, cuyos extractos etanólico y hexánico son capaces de inhibir el crecimiento de bacterias patógenas.

Recent Patents on Impact of Lipopeptide on the Biofilm Formation onto Titanium and Stainless Steel Surfaces

2020 ◽  
Vol 14 (1) ◽  
pp. 49-62
Author(s):  
Mauro Ezio Eustáquio Pires ◽  
Adriano Guimarães Parreira ◽  
Tuânia Natacha Lopes Silva ◽  
Heloísa Carneiro Colares ◽  
José Antonio da Silva ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Stainless Steel ◽  
Bacillus Subtilis ◽  
Biofilm Formation ◽  
Orthopedic Implants ◽  
Stainless Steel Surface ◽  
E Coli ◽  
Subtilis Atcc ◽  
Bacillus Subtilis Atcc ◽  
Steel Surfaces

Background: Numerous causes of infection in arthroplasties are related to biofilm formation on implant surfaces. In order to circumvent this problem, new alternatives to prevent bacterial adhesion biosurfactants-based are emerging due to low toxicity, biodegradability and antimicrobial activity of several biosurfactants. We revised all patents relating to biosurfactants of applicability in orthopedic implants. Methods: This work aims to evaluate the capability of a lipopeptide produced by Bacillus subtilis ATCC 19659 isolates acting as inhibitors of the adhesion of Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 29213 onto titanium and stainless steel surfaces and its antimicrobial activity. Results: The adhesion of the strains to the stainless-steel surface was higher than that of titanium. Preconditioning of titanium and stainless-steel surfaces with 10 mg mL-1 lipopeptide reduced the adhesion of E. coli by up to 93% and the adhesion of S. aureus by up to 99.9%, suggesting the strong potential of lipopeptides in the control of orthopedic infections. The minimal inhibitory concentration and minimum bactericidal concentration were 10 and 240 µg mL-1 for E. coli and S. aureus, respectively. Conclusion: The lipopeptide produced by Bacillus subtilis ATCC 19659 presented high biotechnological application in human health against orthopedic implants infections.

CHEMICAL CONSTITUENTS AND IN-VITRO ANTIMICROBIAL ACTIVITY OF POLYALTHIA STENOPETALA ESSENTIAL OIL

2016 ◽  
Vol 78 (6-8) ◽  
Author(s):  
Noor Izzatie Munira Kamaruddin ◽  
Nor Azah Mohamad Ali ◽  
Muhd Fauzi Safian ◽  
Zaidah Zainal Ariffin
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Gas Chromatography ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Subtilis Atcc ◽  
Bacillus Subtilis Atcc

Polyalthia stenopetala essential oils were extracted by hydrodistillation from the leaves and were analyzed using gas chromatography-flame ionization detector (GC-FID) and gas chromatography/mass spectrometry (GC/MS) system. Thirty one compounds were identified from the analysis. The most abundant components in the leaves oil are curzerene (37.56%) followed by viridiflorol (11.59%), germacrene B (3.77%) and aromadendrene (4.01%). The antimicrobial activity of the oil essential oils was determined with disk diffusion method and minimum inhibitory concentration (MIC) assay. Four bacteria, Staphylococcus aureus (ATCC 25923), Bacillus subtilis (ATCC 6633), Escherichia coli (ATCC 25922) Psedomonas aeruginosa (ATCC 10145) and two yeasts, Candida albicans (ATCC 10231) and Saccharomyces cerevisiae (ATCC 4098) were selected. The crude oil shows the most reactivity against B. subtilis (ATCC 6633) and C. albicans (ATCC 10231) with an inhibitory zone of 11mm. The minimum inhibitory concentration (MIC) of sample against Staphylococcus aureus (ATCC 25923), Bacillus subtilis (ATCC 6633), Escherichia coli (ATCC 25922) Psedomonas aeruginosa (ATCC 10145) and Candida albicans (ATCC 10231) in range of 0.5 mg/ml – 1.0 mg/ml which can categorized as strong.

scholarly journals Synthetic cinnamates as potential antimicrobial agents

2015 ◽  
Vol 69 (1) ◽  
pp. 37-42 ◽  
Author(s):  
Olgica Stefanovic ◽  
Ivana Radojevic ◽  
Ljiljana Comic
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Antimicrobial Agents ◽  
Inhibitory Concentration ◽  
Methyl Cinnamate ◽  
Microdilution Method ◽  
Bacillus Subtilis Atcc ◽  
Better Than

This study deals with synthesis of methyl cinnamate, butyl cinnamate, and p-methoxy methyl cinnamate and testing of their in vitro antimicrobial activity. Antimicrobial activity was examined towards 29 microorganisms using microdilution method. It is shown that antimicrobial activity of methyl cinnamate and p-methoxy methyl cinnamate was better than that of butyl cinnamate. Sarcina lutea, Bacillus subtilis ATCC 6633, B. subtilis and B. subtilis IP 5832 (probiotic) were the most sensitive bacteria. It is established that p-methoxy methyl cinnamate can be a new, potential anti-Staphylococcus aureus agent with minimum inhibitory concentration of 62.5 ?g/ml. Methyl cinnamate and p-methoxy methyl cinnamate inhibited the growth of Aspergillus restrictus, A. flavus and A. fumigatus in the concentration range from 62.5 ?g/ml to 250 ?g/ml.

scholarly journals Composition and evaluation of the antimicrobial activity of the essential oil of Senecio selloi Spreng DC.

2013 ◽  
Vol 15 (4) ◽  
pp. 503-507 ◽  
Author(s):  
G.N.S. Silva ◽  
T.B. Spader ◽  
S.H. Alves ◽  
C.A. Mallmann ◽  
B.M. Heinzmann
Keyword(s):  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Essential Oil ◽  
Bacterial Species ◽  
Major Constituent ◽  
Gram Negative ◽  
Subtilis Atcc ◽  
Aerial Parts ◽  
Sesquiterpene Hydrocarbons ◽  
Bacillus Subtilis Atcc

The essential oil of the aerial parts of Senecio selloi Spreng. DC. was extracted by hydrodistillation and analyzed by GC/MS. Nineteen compounds were identified, representing 99.9% of the total. The main compounds were found to be sesquiterpene hydrocarbons (71.3%), most of them with a bisabolane skeleton (59.4%). The major constituent was α-zingiberene (54%), followed by monoterpene α-isolimonene (16%). The essential oil was also tested against two Gram-positive and two Gram-negative bacterial species, three yeasts, and an algae. From the strains assayed, only Bacillus subtilis ATCC 6633 showed susceptibility (MIC and MBC = 4400 µg/mL) to the essential oil.

Antimicrobial activities of different parts of two geographically distinct varieties of Jatropha curcas Linn. fruit in Pakistan

2021 ◽  
Vol 50 (2) ◽  
pp. 219-226
Author(s):  
Abdul Haq ◽  
Alam Khan ◽  
Zulfiqar Ali Malik ◽  
Mushtaq Ahmed ◽  
Samiullah Khan ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Minimum Inhibitory Concentration ◽  
Seed Coat ◽  
Jatropha Curcas ◽  
Inhibitory Concentration ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Seed Kernel ◽  
Subtilis Atcc ◽  
Bacillus Subtilis Atcc

Antimicrobial activities of deoiled seed kernel (mechanically pressed), fruit coat and seed coat of Jatropha curcas Linn. collected from two regions (Bannu and Peshawar) of Pakistan were investigated. The antimicrobial activities were carried out against Klebsiella pneumoniae (ATCC 43816), Escherichia coli (ATCC 10536), Staphylococcus aureus (ATCC 6538), Bacillus subtilis (ATCC 6633) and two clinical fungal isolates Aspergillus fumigatus and Candida albicans using agar well diffusion method. The antibacterial activities of Peshawar sample were found to be higher than Bannu, against selected strains. While antifungal activities of both samples were similar. Highest zone of inhibition 31.5 ± 0.7 mm was exhibited by n-hexane extract of deoiled seed kernel of Peshawar sample against Bacillus subtilis (ATCC 6633). The minimum inhibitory concentration of ethanolic extracts of deoiled seed kernel and seed coat of Peshawar sample was 31.25 - 25 mg/ml. Whereas, minimum inhibitory concentration of ethanolic and n-hexane extracts of Bannu sample was 62.5 - 125 mg/ml. The results suggested that antimicrobial potential of J. curcas Linn. varied with geographical distribution. The investigation of different varieties of medicinal plants belonging to the same species will greatly enhance the chances of best pharmaceuticals discovery. Bangladesh J. Bot. 50(2): 219-226, 2021 (June)

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