scholarly journals Characterization of immobilized biomass by amplified rDNA restriction analysis (ARDRA) in an anaerobic sequencing-batch biofilm reactor (ASBBR) for the treatment of industrial wastewater

2012 ◽  
Vol 55 (4) ◽  
pp. 623-629 ◽  
Author(s):  
Arnaldo Sarti ◽  
Eloisa Pozzi ◽  
Marcelo Zaiat
Keyword(s):  
Industrial Wastewater ◽  
Restriction Analysis ◽  
Biofilm Reactor ◽  
Sequencing Batch Biofilm Reactor ◽  
Immobilized Biomass ◽  
Amplified Rdna Restriction Analysis

scholarly journals Isolation and Characterization of Mycoplasma mycoides Subspecies capri from Milk of Natural Goat Mastitis Cases

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Vijay Kumar ◽  
Rajneesh Rana ◽  
Somya Mehra ◽  
Pramod Kumar Rout
Keyword(s):  
Restriction Analysis ◽  
Clinical Signs ◽  
Strain Variation ◽  
Specific Pcr ◽  
Isolation And Characterization ◽  
Mycoplasma Mycoides ◽  
Species Specific ◽  
Amplified Rdna Restriction Analysis ◽  
Indian Goat

Association of Mycoplasma mycoides subspecies capri (Mmc) with natural goat mastitis has been studied earlier largely by detecting the Mmc DNA using molecular methods. However, report on detection of cultivable Mmc isolates from natural goat-mastitis milk is still very rare. In this study, Mmc was isolated from milk samples () of goats with or without clinical signs of mastitis. Mmc isolates were further characterized by biochemical and species-specific PCR methods. Intra species strain variation was also studied by 16S amplified rDNA restriction analysis (16S ARDRA). The study recovered a total of 6 Mmc isolates (3.5%). Three types of intraspecies variants among the recovered Mmc isolates were found by 16S ARDRA. The study concluded that Mmc may be an etiological agent of mycoplasmal mastitis in Indian goat herds.

Characterization of cultivated fungi isolated from grape marc wastes through the use of amplified rDNA restriction analysis and sequencing

2010 ◽  
Vol 48 (3) ◽  
pp. 297-306 ◽  
Author(s):  
Spyridon Ntougias ◽  
Nektarios Kavroulakis ◽  
Kalliope K. Papadopoulou ◽  
Constantinos Ehaliotis ◽  
Georgios I. Zervakis
Keyword(s):  
Restriction Analysis ◽  
Grape Marc ◽  
Amplified Rdna Restriction Analysis

scholarly journals Evidence for Phytoremediation and Phytoexcretion of NTO from Industrial Wastewater by Vetiver Grass

Molecules ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 74
Author(s):  
Abhishek RoyChowdhury ◽  
Pallabi Mukherjee ◽  
Saumik Panja ◽  
Rupali Datta ◽  
Christos Christodoulatos ◽  
...  
Keyword(s):  
Stress Reduction ◽  
Industrial Wastewater ◽  
Plant Biomass ◽  
Ionization Mass ◽  
Vetiver Grass ◽  
Insensitive Munitions ◽  
Treatment Technologies ◽  
And Control ◽  
Near Future

The use of insensitive munitions such as 3-nitro-1,2,4-triazol-5-one (NTO) is rapidly increasing and is expected to replace conventional munitions in the near future. Various NTO treatment technologies are being developed for the treatment of wastewater from industrial munition facilities. This is the first study to explore the potential phytoremediation of industrial NTO-wastewater using vetiver grass (Chrysopogon zizanioides L.). Here, we present evidence that vetiver can effectively remove NTO from wastewater, and also translocated NTO from root to shoot. NTO was phytotoxic and resulted in a loss of plant biomass and chlorophyll. The metabolomic analysis showed significant differences between treated and control samples, with the upregulation of specific pathways such as glycerophosphate metabolism and amino acid metabolism, providing a glimpse into the stress alleviation strategy of vetiver. One of the mechanisms of NTO stress reduction was the excretion of solid crystals. Scanning electron microscopy (SEM), electrospray ionization mass spectrometry (ESI-MS), and Fourier-transform infrared spectroscopy (FTIR) analysis confirmed the presence of NTO crystals in the plant exudates. Further characterization of the exudates is in progress to ascertain the purity of these crystals, and if vetiver could be used for phytomining NTO from industrial wastewater.

Nitrogen removal from livestock and poultry breeding wastewaters using a novel sequencing batch biofilm reactor

2010 ◽  
Vol 62 (11) ◽  
pp. 2599-2606 ◽  
Author(s):  
Hong Xiao ◽  
Ping Yang ◽  
Hong Peng ◽  
Yanzong Zhang ◽  
Shihuai Deng ◽  
...  
Keyword(s):  
Nitrogen Removal ◽  
Heterotrophic Bacteria ◽  
Biofilm Reactor ◽  
N Accumulation ◽  
Sequencing Batch Biofilm Reactor ◽  
Poultry Breeding ◽  
Mode 2 ◽  
Do Concentration ◽  
Nitrification And Denitrification ◽  
Biological Nitrogen

A study was conducted regarding the biological nitrogen removal from the livestock and poultry breeding wastewater (LPBWs) using a novel sequencing batch biofilm reactor (SBBR). Nitrogen removal process was studied under three aeration strategies/modes, referred to as MODE 1, 2, and 3. The results showed that MODE 2 (one operation period: instant fill of LPBWs, 3.0 h aeration, 1.5 h non-aeration, 1.5 h aeration, 1.0 h non-aeration and rapid drain of treated LPBWs) performed the best in nitrogen removal. Under MODE 2, the removal efficiencies were as high as 96.1 and 92.1% for NH3-N and TN, respectively. Simultaneous nitrification and denitrification (SND), as well as shortcut nitrification and denitrification are likely to be the two main mechanisms for the nitrogen removal in this study. Nitrifying bateria were not inhibited by heterotrophic bacteria with C/N ratios ranging from 18.1 to 21.4 and DO concentration of 2.0 mg/l. Alternation between aeration and non-aeration played an important role in NO2−-N accumulation.

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