scholarly journals Antimicrobial resistance and PCR-ribotyping of Shigella responsible for foodborne outbreaks occurred in southern Brazil

2010 ◽  
Vol 41 (4) ◽  
pp. 966-977 ◽  
Author(s):  
Cheila Minéia Daniel de Paula ◽  
Mercedes Passos Geimba ◽  
Patrícia Heidrich do Amaral ◽  
Eduardo Cesar Tondo
Keyword(s):  
Antimicrobial Resistance ◽  
Southern Brazil ◽  
Foodborne Outbreaks ◽  
Pcr Ribotyping

Spread of a Major Clone of Salmonella enterica Serotype Enteritidis in Poultry and in Salmonellosis Outbreaks in Southern Brazil

2016 ◽  
Vol 80 (1) ◽  
pp. 158-163 ◽  
Author(s):  
KAREN APELLANIS BORGES ◽  
THALES QUEDI FURIAN ◽  
SARA NEVES de SOUZA ◽  
EDUARDO CÉSAR TONDO ◽  
ANDRÉ FELIPE STRECK ◽  
...  
Keyword(s):  
Salmonella Enteritidis ◽  
Food Sources ◽  
Southern Brazil ◽  
Salmonella Spp ◽  
Clonal Relationship ◽  
Poultry Products ◽  
The World ◽  
Foodborne Outbreaks ◽  
Major Clone ◽  
Pcr Ribotyping

ABSTRACT Salmonella spp. are among the most important agents of foodborne diseases all over the world. Human Salmonella outbreaks are often associated with the consumption of poultry products (meat and eggs), and one of the most prevalent serotypes associated with these products is Salmonella Enteritidis. Brazil is one of the most important poultry exporters in the world. In southern Brazil, three closely related clones of Salmonella Enteritidis have been responsible for the majority of foodborne Salmonella outbreaks over the past decade. However, until now, there has been little information regarding the clonal relationship among the Brazilian Salmonella strains of avian origin and those involved in foodborne outbreaks. Therefore, the aim of the present study was to complete the molecular characterization of Salmonella Enteritidis strains isolated from poultry and food sources involved in Salmonella outbreaks. PCR ribotyping was performed to discriminate the strains into different ribotype profiles according to the banding pattern amplification. This technique was able to differentiate the Salmonella Enteritidis strains into two banding patterns: R2 and R4. R2 accounted for 98.7% of the strains. DNA sequencing of the 600-bp fragment, present in all ribotypes, was applied to confirm this result. The sequences generated showed high levels of similarity, ranging from 99.7 to 100%, and were grouped into a single cluster. These results suggest that there is a clonal relationship among the Salmonella Enteritidis strains responsible for several salmonellosis outbreaks and the strains collected from poultry sources.

scholarly journals Salmonella serotypes, resistance patterns, and food vehicles of salmonellosis in southern Brazil between 2007 and 2012

2014 ◽  
Vol 8 (07) ◽  
pp. 811-817 ◽  
Author(s):  
Roberta Capalonga ◽  
Rosane Campanher Ramos ◽  
Jane Mari Correa Both ◽  
Mara Lúcia Tiba Soeiro ◽  
Solange Mendes Longaray ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Rio Grande ◽  
Foodborne Diseases ◽  
Southern Brazil ◽  
Rio Grande Do Sul ◽  
Central Laboratory ◽  
Resistance Patterns ◽  
Foodborne Outbreaks ◽  
Salmonella Serotypes ◽  
Official Records

Introduction: Previous studies have identified Salmonella as the main causative agent of foodborne diseases in the state of Rio Grande do Sul (RS), southern Brazil, between 1997 and 2006. This study aimed to describe the Salmonella serotypes, antimicrobial patterns, and food vehicles of salmonellosis that occurred in RS between 2007 and 2012. Methodology: Information about Salmonella isolates and salmonellosis outbreaks registered in the official records of the Central Laboratory of RS (FEEPS/IPB-LACEN/RS) was analyzed. Results: Among the 163 isolates investigated, 138 (84.7%) were identified as S. Enteritidis. The second and third most frequent serovars identified were S. Schwarzengrund (5.5%) and S. Typhimurium (3.7%). Homemade mayonnaise was the food vehicle most frequently identified (17.39%), followed by pastry products (15.94%) and beef (12.32%). Antimicrobial resistance was analyzed; 12 drugs were tested. Higher percentages of resistance were observed to nitrofurantoin (94.2%) and nalidixic acid (89.1%). The resistance to these two drugs was verified in 80.43% of the isolates. Multi-resistance to three and five drugs was verified in four and two isolates, respectively. Conclusions: Comparing the results of the present study with results of previous reports, it was possible to conclude that S. Enteritidis and homemade mayonnaise are still the main serotype and food vehicle of salmonellosis in RS and that antimicrobial resistance has been increasing among S. Enteritidis responsible for foodborne outbreaks in southern Brazil.

scholarly journals Antimicrobial resistance profile of Enterococcus spp isolated from food in Southern Brazil

2009 ◽  
Vol 40 (1) ◽  
pp. 125-128 ◽  
Author(s):  
Gustavo Pelicioli Riboldi ◽  
Jeverson Frazzon ◽  
Pedro Alves d'Azevedo ◽  
Ana Paula Guedes Frazzon
Keyword(s):  
Antimicrobial Resistance ◽  
Southern Brazil ◽  
Resistance Profile ◽  
Antimicrobial Resistance Profile ◽  
Enterococcus Spp

Carbapenem-susceptible Acinetobacter baumannii carrying the ISAba1 upstream blaOXA-51-like gene in Porto Alegre, southern Brazil

2012 ◽  
Vol 141 (2) ◽  
pp. 330-333 ◽  
Author(s):  
M. PAGANO ◽  
A. F. MARTINS ◽  
A. B. M. P. MACHADO ◽  
J. BARIN ◽  
A. L. BARTH
Keyword(s):  
Antimicrobial Resistance ◽  
Acinetobacter Baumannii ◽  
Clinical Isolates ◽  
High Morbidity ◽  
Southern Brazil ◽  
Porto Alegre ◽  
Integrase Gene ◽  
Carbapenem Resistant ◽  
High Prevalence ◽  
Class 1

SUMMARYOver the last decade, Acinetobacter baumannii resistant to carbapenems has emerged in many medical centres and is commonly associated with high morbidity and mortality. We investigated potential mechanisms contributing to antimicrobial resistance of 58 clinical isolates of A. baumannii collected during a prolonged city-wide outbreak in five different hospitals in southern Brazil. The integrase gene was detected in 51 (87·9%) isolates of which 36 harboured class 2 integrons alone and 14 had both class 1 and 2 integrons; all carbapenem-resistant isolates displayed class 2 integrons. ISAba1 was found upstream of blaOXA-23-like only in isolates resistant to carbapenems; however, ISAba1 upstream of blaOXA-51-like was present in both susceptible and resistant isolates. This is the first report of a high prevalence of class 2 integrons in A. baumannii in southern Brazil. Moreover, our study suggests that ISAba1/blaOXA-51-like alone is insufficient to confer resistance to carbapenems.

Clonal relationship among Salmonella enterica serovar Enteritidis involved in foodborne outbreaks in Southern Brazil

Food Control ◽  
2009 ◽  
Vol 20 (6) ◽  
pp. 606-610 ◽  
Author(s):  
Fernanda A. de Oliveira ◽  
Mercedes P. Geimba ◽  
Ana P. Pasqualotto ◽  
Adriano Brandelli ◽  
Giancarlo Pasquali ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Southern Brazil ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Clonal Relationship ◽  
Foodborne Outbreaks

scholarly journals Antimicrobial resistance profiles of Staphylococcus aureus clusters on small dairy farms in southern Brazil

2016 ◽  
Vol 36 (10) ◽  
pp. 951-956 ◽  
Author(s):  
Lilian K. Girardini ◽  
◽  
Daniel S. Paim ◽  
Thais C. Ausani ◽  
Graciela V. Lopes ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Dairy Farms ◽  
Diffusion Method ◽  
Dairy Herds ◽  
Southern Brazil ◽  
Disk Diffusion Method ◽  
Banding Patterns ◽  
Lactating Cows ◽  
Resistance To Penicillin ◽  
Total Dna

ABSTRACT: In intensive dairy farming, persistent intramammary infection has been associated with specific Staphylococcus (S.) aureus strains, and these strains may be resistant to antimicrobials. The objective of this study was to evaluate the antimicrobial resistance phenotypes of S. aureus isolates and to assess the distribution and the persistence of clonal groups in small dairy herds of southern Brazil. Milk samples were collected from all lactating cows from 21 dairy farms over a two-year period, totaling 1,060 samples. S. aureus isolates were tested for susceptibility to thirteen antimicrobials using the disk diffusion method. The total DNA of the isolates was subjected to SmaI digestion followed by pulsed-field gel electrophoresis (PFGE). Banding patterns differing by ≤4 bands were considered members of a single PFGE cluster. The frequency of S. aureus isolation ranged from 3.45% to 70.59% among the 17 S. aureus-positive herds. Most S. aureus isolates (87.1%) were susceptible to all antimicrobials; resistance to penicillin (18.2%) was the most frequently observed. The 122 isolates subjected to macrorestriction analysis were classified into 30 PFGE-clusters. Among them, only 10 clusters were intermittent or persistent over the two-year period. The majority (93.6%) of isolates belonging to persistent and intermittent clusters were susceptible to all tested antimicrobials. S. aureus intramammary colonization in small dairy farms of southern Brazil is most frequently caused by sporadic PFGE clusters, although some persistent clusters can arise over time. Both sporadic and persistent isolates were highly susceptible to antimicrobials.

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