Spread of a Major Clone of Salmonella enterica Serotype Enteritidis in Poultry and in Salmonellosis Outbreaks in Southern Brazil

2016 ◽  
Vol 80 (1) ◽  
pp. 158-163 ◽  
Author(s):  
KAREN APELLANIS BORGES ◽  
THALES QUEDI FURIAN ◽  
SARA NEVES de SOUZA ◽  
EDUARDO CÉSAR TONDO ◽  
ANDRÉ FELIPE STRECK ◽  
...  
Keyword(s):  
Salmonella Enteritidis ◽  
Food Sources ◽  
Southern Brazil ◽  
Salmonella Spp ◽  
Clonal Relationship ◽  
Poultry Products ◽  
The World ◽  
Foodborne Outbreaks ◽  
Major Clone ◽  
Pcr Ribotyping

ABSTRACT Salmonella spp. are among the most important agents of foodborne diseases all over the world. Human Salmonella outbreaks are often associated with the consumption of poultry products (meat and eggs), and one of the most prevalent serotypes associated with these products is Salmonella Enteritidis. Brazil is one of the most important poultry exporters in the world. In southern Brazil, three closely related clones of Salmonella Enteritidis have been responsible for the majority of foodborne Salmonella outbreaks over the past decade. However, until now, there has been little information regarding the clonal relationship among the Brazilian Salmonella strains of avian origin and those involved in foodborne outbreaks. Therefore, the aim of the present study was to complete the molecular characterization of Salmonella Enteritidis strains isolated from poultry and food sources involved in Salmonella outbreaks. PCR ribotyping was performed to discriminate the strains into different ribotype profiles according to the banding pattern amplification. This technique was able to differentiate the Salmonella Enteritidis strains into two banding patterns: R2 and R4. R2 accounted for 98.7% of the strains. DNA sequencing of the 600-bp fragment, present in all ribotypes, was applied to confirm this result. The sequences generated showed high levels of similarity, ranging from 99.7 to 100%, and were grouped into a single cluster. These results suggest that there is a clonal relationship among the Salmonella Enteritidis strains responsible for several salmonellosis outbreaks and the strains collected from poultry sources.

Incidence of Salmonella, Campylobacter jejuni, Campylobacter coli, and Listeria monocytogenes in Poultry Carcasses and Different Types of Poultry Products for Sale on the Belgian Retail Market

1999 ◽  
Vol 62 (7) ◽  
pp. 735-740 ◽  
Author(s):  
M. UYTTENDAELE ◽  
P. DE TROY ◽  
J. DEBEVERE
Keyword(s):  
Listeria Monocytogenes ◽  
Campylobacter Jejuni ◽  
Broiler Chickens ◽  
Salmonella Enteritidis ◽  
Contamination Level ◽  
Contamination Rate ◽  
Campylobacter Coli ◽  
Retail Market ◽  
Salmonella Spp ◽  
Poultry Products

From January 1997 to May 1998, 772 samples of poultry carcasses and poultry products for sale on the retail market in Belgium were analyzed for the presence of Salmonella spp., Salmonella Enteritidis, Campylobacter jejuni, C. coli, and Listeria monocytogenes per 100 cm2 or 25 g. Poultry samples were contaminated with Salmonella (36.5%), C. jejuni and C. coli (28.5%), and L. monocytogenes (38.2%). In about 12.3% of the poultry samples, the L. monocytogenes contamination level exceeded 1 CFU per g or cm2. Significant differences in pathogen contamination rates of poultry products were noticed between the poultry products originating from Belgian, French, and U.K. abattoirs. Poultry products derived from broiler chickens running free in pine woods until slaughtering age (12 to 13 weeks) had a significantly (P < 0.05) lower contamination rate of Salmonella than poultry products from enclosed broilers slaughtered at the age of 6 to 8 weeks. A significantly (P < 0.05) lower pathogen contamination rate was noted for Salmonella, C. jejuni, and C. coli for poultry cuts without skin compared to poultry cuts with skin on. An increase in pathogen contamination rate was noticed during cutting and further processing. To diminish C. jejuni, C. coli, Salmonella, and L. monocytogenes contamination rates, hygienic rules of slaughter and meat processing must be rigorously observed. At the moment, zero tolerance for these pathogens is not feasible, and there is a need to establish criteria allowing these pathogens to be present at reasonable levels in the examined poultry samples.

scholarly journals Antimicrobial resistance and PCR-ribotyping of Shigella responsible for foodborne outbreaks occurred in southern Brazil

2010 ◽  
Vol 41 (4) ◽  
pp. 966-977 ◽  
Author(s):  
Cheila Minéia Daniel de Paula ◽  
Mercedes Passos Geimba ◽  
Patrícia Heidrich do Amaral ◽  
Eduardo Cesar Tondo
Keyword(s):  
Antimicrobial Resistance ◽  
Southern Brazil ◽  
Foodborne Outbreaks ◽  
Pcr Ribotyping

scholarly journals Salmonella Enteritidis in chicken carcasses and giblets in Southern Chile

2009 ◽  
Vol 4 (02) ◽  
pp. 107-109 ◽  
Author(s):  
Jorge Ulloa ◽  
Mario Gonzalez ◽  
Carlos Hernandez ◽  
Maria Paz Villanueva ◽  
Heriberto Fernandez
Keyword(s):  
Salmonella Enteritidis ◽  
Low Frequency ◽  
Chicken Meat ◽  
Southern Chile ◽  
Salmonella Infection ◽  
Salmonella Spp ◽  
Isolation Frequency ◽  
Poultry Products ◽  
Raw Poultry

Background: Gastroenteritis caused by Salmonella spp. is mainly related to the consumption of undercooked chicken meat or raw poultry products. The objective of this study was to assess the occurrence of Salmonella spp. in chicken carcasses and giblets commercialized in Southern Chile (Valdivia city). Methodology: A total of 560 samples were collected from four supermarkets and one poultry products dealer, during two periods (autumn-winter and spring-summer periods), and analysed for Salmonella using standard bacteriological procedures. Results: Five out of 280 analyzed carcasses (1.8%) and one out of 280 (0.4%) chicken giblets were Salmonella positive. The isolation frequency of Salmonella spp. considering the total samples analyzed was 1.1%. Salmonella Enteritidis was the only serotype isolated. No significant differences (P > 0.05) in the isolation rates between the autumn-winter and spring-summer periods were found. Conclusion: Salmonella Enteritidis was isolated in low frequency from chicken carcasses and giblets commercialized in Southern Chile (Valdivia city); however, in spite of the low frequency of contaminated samples, this kind of food could be a potential vehicle of Salmonella infection to humans.

Clonal relationship among Salmonella enterica serovar Enteritidis involved in foodborne outbreaks in Southern Brazil

Food Control ◽  
2009 ◽  
Vol 20 (6) ◽  
pp. 606-610 ◽  
Author(s):  
Fernanda A. de Oliveira ◽  
Mercedes P. Geimba ◽  
Ana P. Pasqualotto ◽  
Adriano Brandelli ◽  
Giancarlo Pasquali ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Southern Brazil ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Clonal Relationship ◽  
Foodborne Outbreaks

scholarly journals Whole-Genome Analysis of Multidrug-Resistant Salmonella Enteritidis Strains Isolated from Poultry Sources in Korea

Pathogens ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1615
Author(s):  
Tae-Min La ◽  
Taesoo Kim ◽  
Hong-Jae Lee ◽  
Joong-Bok Lee ◽  
Seung-Yong Park ◽  
...  
Keyword(s):  
Salmonella Enteritidis ◽  
Clinical Signs ◽  
Food Poisoning ◽  
Multidrug Resistant ◽  
Common Source ◽  
Whole Genome ◽  
Salmonella Spp ◽  
Whole Genome Analysis ◽  
Poultry Products ◽  
Antibiotic Susceptibility Test

The Salmonella Enterica subsp. Enterica serovar Enteritidis is one of main serovars isolated from human patients with food poisoning and poultry without clinical signs. Consumption of poultry products contaminated with Salmonella Enteritidis is a common source of human salmonellosis; 82 Salmonella spp. were isolated from 291 samples of retail chicken meat, 201 one-day-old chicks, 30 internal organs of chickens, 156 chicken eggs, 100 duck eggs, 38 straw bedding samples, 18 samples of retail duck meat, and 19 swab samples from slaughterhouses in 2019 and 2020. An antibiotic susceptibility test was performed for all isolates, revealing 33 multidrug-resistant (MDR) strains. The whole genome of 33 MDR strains isolated in 2019 and 2020 and 10 strains isolated in 2011, 2012, and 2017 was sequenced using the MinION sequencing protocol. Within these 43 samples, 5 serovars were identified: S. Enteritidis, S. Agona, S. Virchow, S. Albany, and S. Bareilly. The most common serovar was S. Enteritidis (26/43), which showed the highest resistance to ampicillin (100%), followed by nalidixic acid (90%) and colistin (83%). Core genome multilocus sequence typing analysis showed that the S. Enteritidis strains isolated from different sources and in different years were clustered together. In addition, the S. Enteritidis strains isolated since 2011 consistently harbored the same antibiotic resistance patterns.

scholarly journals CONTROL METHODS OF THE RED CHICKEN MITE

2020 ◽  
Vol 21 (2) ◽  
pp. 11-17
Author(s):  
I. K. Avdosieva ◽  
S. А. Ponomareva ◽  
V. M. Malynivsky ◽  
L. I. Flyak
Keyword(s):  
Salmonella Enteritidis ◽  
Dermanyssus Gallinae ◽  
Economic Damage ◽  
Control Methods ◽  
Salmonella Gallinarum ◽  
Poultry Products ◽  
Wide Range ◽  
The World ◽  
Methods Of Control

One of the most dangerous ectoparasites that infects poultry is the red chicken mite (Dermanyssus gallinae), which causes dermanisiosis, which is widespread in many countries around the world, including all regions of Ukraine. The article shows the basic biological and ecological characteristics of the red chicken mite Dermanyssus gallinae. gallinae is a carrier of infectious and viral diseases including: Salmonella gallinarum, Salmonella enteritidis, Chlamydia spp., Borrelia anserine rhusiopathiae, Listeria monocytogenes, Coxiella burnetii, Escherichia coli, Staphylococcus sрр., Marek's disease, Newcastle disease and other dangerous poultry diseases. gallinae causes significant economic damage due to reduced egg productivity, quality of poultry products and increased culling and death of poultry due to depletion. The article analyzes the market of insecticides registered in Ukraine for the control of red mites. The effectiveness of preventive and curative measures in the battle against D. gallinae largely depends on the method and desacarization medication. Among the existing methods of control against D. gallinae remains chemical because synthetic insecticides have a wide range of action, while destroying a number of pests from different groups at different stages of development. To prevent outbreaks and spread of mites in poultry farms, it is necessary to systematically implement a set of organizational and veterinary measures, taking into account the biological characteristics of the development of existing ectoparasites in the farm and technological cycles of poultry.

Investigation of rpoS and dps Genes in Sodium Hypochlorite Resistance of Salmonella Enteritidis SE86 Isolated from Foodborne Illness Outbreaks in Southern Brazil

2012 ◽  
Vol 75 (3) ◽  
pp. 437-442 ◽  
Author(s):  
ANA CAROLINA RITTER ◽  
DONATELLA BACCIU ◽  
LUCÉLIA SANTI ◽  
WALTER ORLANDO BEYS da SILVA ◽  
MARILENE HENNING VAINSTEIN ◽  
...  
Keyword(s):  
Sodium Hypochlorite ◽  
Salmonella Enteritidis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Southern Brazil ◽  
Rio Grande Do Sul ◽  
Wild Type ◽  
Log Reduction ◽  
The World ◽  
Mutant Strains

In Rio Grande do Sul, southern Brazil, Salmonella Enteritidis is one of the principal microorganisms responsible for foodborne disease. The present study was conducted to compare the sodium hypochlorite resistance of Salmonella Enteritidis SE86 with that of other strains of Salmonella Enteritidis isolated from different regions of the world and to investigate the involvement of the rpoS and dps genes in resistance to this disinfectant. We tested five Salmonella Enteritidis wild-type (WT) strains isolated from different countries, two mutant strains of Salmonella Enteritidis SE86, and two tagged (3XFLAG) strains of Salmonella Enteritidis SE86 for their resistance to sodium hypochlorite (200 ppm). The survival of the WT and attenuated strains was determined based on bacterial counts, and tagged proteins (Dps and RpoS) were detected by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and immunoblotting with anti-FLAG antibodies. None of the WT strains of Salmonella Enteritidis were totally inactivated after 20 min. The SE86 strain lacking dps was more sensitive to sodium hypochlorite than was the WT SE86 strain, with a 2-log reduction in counts after 1 min. The RpoS and Dps proteins were actively expressed under the conditions tested, indicating that in Salmonella Enteritidis SE86 these genes, which are expressed when in contact with sodium hypochlorite, are related to oxidative stress.

scholarly journals Isolation of Bacteriophage from Eggs and Use of Excreta as Biocontrol Agent in Controlling Eggs Related Salmonellosis

2019 ◽  
Vol 35 (1) ◽  
pp. 37-44
Author(s):  
Md Abu Sayem Khan ◽  
Md Muktadir Rahman Ashik ◽  
Sabita Rezwana Rahman
Keyword(s):  
Salmonella Enteritidis ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Penicillin G ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Antibiotic Resistant ◽  
Poultry Products ◽  
Wide Range ◽  
Agar Layer

Poultry and poultry products are the leading causes of foodborne salmonellosis worldwide. Antibiotics are used to control Salmonella spp. in poultry but its uncontrolled use results in the emergence of resistant pathogens.The use of bacteriophages as antimicrobial agents to control antibiotic resistant pathogenic bacteria could be a possible alternative. The aim of this study was to isolate, characterize and evaluate the effectiveness of bacteriophages for reducingload of Salmonella spp. on eggshells. One bacteriophage named as Sal-PE, specific to Salmonella enteritidiswas isolated from poultry excreta. For isolation, samples were subject to an enrichment protocol and then double agar layer method was performed to detect plaque. It had the capability to survive in wide range of pH between 4 to 10and found to be resistant at 60°C for 1 hour. Sal” PE showed its lytic effect on 13 of the 15 (87%) isolates including Salmonella enteritidis and Salmonella typhimurium which were recovered from 50 poultry excreta samples. After enrichment and growth on selective media, isolates were identified based on cultural characteristics, microscopic observation and biochemical tests. Amplification of three different genes (invA, sdfI, fliC) were carried out tocharacterize those isolates in molecular level. All isolates were found to be resistant to penicillin G, ampicillin, oxacillin and clindamycin but sensitive to ciprofloxacin, streptomycin, cefixime and chloramphenicol. Lytic efficiency of Sal-PE was determined by observing the reduction in optical density due to destruction of pathogens. Though more studies are needed in order to evaluate phage effectiveness, our findingsare expected to help us in initiating the development of a better preventive approach to control the occurrence of Salmonella spp. on eggshells. Bangladesh J Microbiol, Volume 35 Number 1 June 2018, pp 37-44

scholarly journals Biofilm Formation by Salmonella Enteritidis at Different Incubation Temperatures

2018 ◽  
Vol 47 (1) ◽  
Author(s):  
Laura Beatriz Rodrigues ◽  
Bruna Webber ◽  
Rafael Levandowski ◽  
Sarah Souza Gehlen ◽  
Luciana Ruschel dos Santos ◽  
...  
Keyword(s):  
Low Temperatures ◽  
Biofilm Formation ◽  
Salmonella Enteritidis ◽  
Animal Feed ◽  
Food Preservation ◽  
Bacterial Suspension ◽  
Poultry Products ◽  
Biofilm Production ◽  
Foodborne Outbreaks ◽  
Stool Cultures

Background: The genus Salmonella, associated with poultry products, is considered the leading cause of foodborne outbreaks in humans in many countries. In Brazil, Salmonella Enteritidis (SE) is the serovar remains as one most frequently isolated from humans, and it is also a major serovar found in animals, food, animal feed, and environmental samples, despite all the efforts to control this pathogen. Also, the bacterium is able to form biofilms on different surfaces, protecting cells from both cleaning and sanitizing procedures in the food industries. This study aimed to verify the ability of Salmonella Enteritidis isolates to form biofilm on polystyrene at different incubation temperatures.Materials, Methods & Results: A total of 171 SE samples were isolated from foodborne outbreaks (foods and stool cultures) and poultry products between 2003 and 2010. The biofilm-forming ability of samples was measured at four different temperatures (3°C, 9ºC, 25ºC, and 36ºC), for 24 h, simulating temperatures usually found in poultry slaughterhouses. Later, 200 μL of each bacterial suspension was inoculated, in triplicate, onto 96-well, flat-bottomed sterile polystyrene microtiter plates, washed, after that, the biofilm was fixed with methanol. The plates were dried at ambient temperature, stained with 2% Hucker’s crystal violet. Afterwards, absorbance was read using an ELISA plate reader and the optical density (OD) of each isolate was obtained by the arithmetic mean of the absorbance of three wells and this value was compared with the mean absorbance of negative controls (ODnc). The following classification was used for the determination of biofilm formation: no biofilm production, weak biofilm production, moderate biofilm production and strong biofilm production. Results demonstrated all isolates from stool cultures and foods involved in foodborne outbreaks, at least one of the four temperatures tested, were able to form biofilm, even at 3°C, undescribed as possible for the growth of SE. SE strains from poultry products also formed biofilm at least at one of the temperatures.Discussion: the prevention of biofilms formation is very important, once they can be difficult to remove from utensils and food equipment surfaces, becoming a chronic source of microbial contamination of foods, possible dissemination of diseases, and increase of resistance to cleaning and sanitization procedures. A high ability for biofilm formation on plastic surfaces was observed. We may consider that Salmonella has the capacity to bind to surfaces, with relevant impacts on public health. Although biofilm formation could be affected by temperature, most of the SE isolates analyzed in our study were strong biofilm producers at all temperatures, including at 3°C, a temperature used for food preservation and until then not acknowledged as worrisome regarding the development of Salmonella spp. There is a common sense that maintenance of food at low temperatures, particularly below 5°C, is safer to consumers as low temperatures reduce microbial multiplication. However, our results show that the growth of SE in its sessile form is possible under refrigeration. These findings lead to the assumption that the ability of SE to form biofilms, especially at low temperatures, is related to its endurance in inhospitable environments, eventually infecting humans, and that may be one of the factors associated with the high prevalence of this serovar in outbreaks of foodborne diseases. To our knowledge, this is the first publication about biofilm formation by Salmonella Enteritidis at 3ºC.

scholarly journals Antimicrobial Resistance Profiling of Biofilm Forming Non Typhoidal Salmonella enterica Isolates from Poultry and Its Associated Food Products from Pakistan

Antibiotics ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 785
Author(s):  
Abubakar Siddique ◽  
Sara Azim ◽  
Amjad Ali ◽  
Saadia Andleeb ◽  
Aitezaz Ahsan ◽  
...  
Keyword(s):  
Public Health ◽  
Antimicrobial Resistance ◽  
Biofilm Formation ◽  
Salmonella Enteritidis ◽  
Multiple Drug Resistance ◽  
Food Products ◽  
Health Concern ◽  
Multiple Drug ◽  
Zoonotic Potential ◽  
Salmonella Spp

Salmonellosis caused by non-typhoidal Salmonellaenterica from poultry products is a major public health concern worldwide. This study aimed at estimating the pathogenicity and antimicrobial resistance in S. enterica isolates obtained from poultry birds and their food products from different areas of Pakistan. In total, 95/370 (25.67%) samples from poultry droppings, organs, eggs, and meat were positive for Salmonella. The isolates were further identified through multiplex PCR (mPCR) as Salmonella Typhimurium 14 (14.7%), Salmonella Enteritidis 12 (12.6%), and other Salmonella spp. 69 (72.6%). The phenotypic virulence properties of 95 Salmonella isolates exhibited swimming and/or swarming motility 95 (100%), DNA degrading activity 93 (97.8%), hemolytic activity 92 (96.8%), lipase activity 87 (91.6%), and protease activity 86 (90.5%). The sopE virulence gene known for conferring zoonotic potential was detected in S. Typhimurium (92.8%), S. Enteritidis (100%), and other Salmonella spp. (69.5%). The isolates were further tested against 23 antibiotics (from 10 different antimicrobial groups) and were found resistant against fifteen to twenty-one antibiotics. All isolates showed multiple drug resistance and were found to exhibit a high multiple antibiotic-resistant (MAR) index of 0.62 to 0.91. The strong biofilm formation at 37 °C reflected their potential adherence to intestinal surfaces. There was a significant correlation between antimicrobial resistance and the biofilm formation potential of isolates. The resistance determinant genes found among the isolated strains were blaTEM-1 (59.3%), blaOxA-1 (18%), blaPSE-1 (9.5%), blaCMY-2 (43%), and ampC (8.3%). The detection of zoonotic potential MDR Salmonella in poultry and its associated food products carrying cephalosporin and quinolone resistance genes presents a major threat to the poultry industry and public health.

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