scholarly journals DNA fragmentation and membrane damage of bocachico Prochilodus magdalenae (Ostariophysi: Prochilodontidae) sperm following cryopreservation with dimethylsulfoxide and glucose

2012 ◽  
Vol 10 (3) ◽  
pp. 577-586 ◽  
Author(s):  
José Gregorio Martínez ◽  
Víctor Atencio García ◽  
Sandra Pardo Carrasco
Keyword(s):  
Dna Fragmentation ◽  
Ex Situ Conservation ◽  
Sperm Quality ◽  
Membrane Damage ◽  
Egg Yolk ◽  
Ex Situ ◽  
Important Species ◽  
Chicken Egg ◽  
Nitrogen Vapor ◽  
Chicken Egg Yolk

The endangered bocachico Prochilodus magdalenae is a native freshwater fish of Colombia, the most captured species locally and one of the most important species for ex-situ conservation (germplasm banks). The aim of this study was to examine the effect of three concentrations of Dimethylsulfoxide (DMSO) (5%, 10%, 15%) and three of glucose (305, 333, 361 mM) in the extender on spermatic DNA fragmentation (F-DNA) (by Halomax®, Chromatin dispersion) and membrane damage (D-Me) (by eosin-nigrosin staining). After assessment of sperm quality by computer analysis of motility, one part of semen from males was diluted separately with three parts of extender and filled into 0.5 ml straws. Freezing was carried out in liquid nitrogen vapor dry shipper for 30 minutes and thawed at 60ºC for 8 seconds in a water bath and evaluated for the percentage of cells found with F-DNA and D-Me. The results demonstrated that cryopreservation causes greater F-DNA (13.62 ± 1.6% to 28.91 ± 3.25) and D-Me (24.27 ± 1.1% to 58.33 ± 2.81%) when compared with pre-freezing semen (PFS) (6.71 ± 1.54% and 2.34 ± 0.5%, respectively for F-DNA and D-Me). A significant interaction was found between DMSO and glucose concentration in this experiment. Use of extender: 10% DMSO + 305 mM glucose + 12% chicken egg yolk and, 10% DMSO + 333 mM glucose + 12% chicken egg yolk, allow for lower F-DNA and D-Me during cryopreservation of bocachico semen. A high correlation between F-DNA and D-Me was found (r = 0.771).

scholarly journals CRYOPRESERVATION OF KAMPUNG ROOSTER SEMEN USING EGG YOLK DILUENT FROM FOUR TYPES OF POULTRY WITH DIFFERENT CONCENTRATIONS

2019 ◽  
Vol 13 (3) ◽  
Author(s):  
Khairuddin Khairuddin ◽  
Muhammad Erik Kurniawan ◽  
Soman Soman
Keyword(s):  
Liquid Nitrogen ◽  
Recovery Rate ◽  
Egg Yolk ◽  
Spermatozoa Motility ◽  
Chicken Egg ◽  
Nitrogen Vapor ◽  
Before And After ◽  
Egg Yolks ◽  
Chicken Egg Yolk

The aim of this study was to determine the type and the best concentration of egg yolk in maintaining the quality of kampung rooster spermatozoa during cryopreservation. This study used a completely randomized factorial pattern design with the first factor was the type of egg yolk (purebred chicken, kampung chicken, duck, and quail) and the second factor was the concentration of egg yolk (5%, 10%, and 15%). Semen was collected from twelve kampung roosters using massage method. Immediately after collection, the semen was evaluated macroscopically and microscopically. Semen with more than 70% motility was used in this study. The semen was diluted, packed in a ministraw, equilibrated, and frozen using liquid nitrogen vapor and stored in a liquid nitrogen container for 24 hours. Observation of spermatozoa motility was carried out in fresh semen, diluted semen, after equilibration and after thawing with four replications. The results showed that the type of egg yolk treatment had no effect (P0.05) on the recovery rate and motility of spermatozoa before and after cryopreservation, but egg yolk concentration had a highly significant effect (P0.01) on the quality of spermatozoa. Egg yolks in 10-15% concentration had spermatozoa motility and recovery rate higher than egg yolk with 5% concentration. In conclusion, purebred chicken egg yolk, kampung chicken egg yolk, duck egg yolk, and quail egg yolk each in diluent can be used to maintain the quality of kampung rooster spermatozoa at a concentration of 10-15% during cryopreservation.

scholarly journals A diluent containing coconut water, fructose, and chicken egg yolk increases rooster sperm quality at 5°C

2019 ◽  
Vol 12 (7) ◽  
pp. 1116-1120 ◽  
Author(s):  
Siti Eliana Rochmi ◽  
Miyayu Soneta Sofyan
Keyword(s):  
Sperm Quality ◽  
Sperm Concentration ◽  
Egg Yolk ◽  
Mean Value ◽  
Coconut Water ◽  
Sperm Viability ◽  
Spermatozoa Motility ◽  
Chicken Egg ◽  
Semen Volume ◽  
Chicken Egg Yolk

Aim: The present study was conducted to evaluate the quality of rooster sperm at 5°C after treatment with a diluent containing coconut water, fructose, and chicken egg yolk and stored the semen sample at 5°C. Materials and Methods: Ten semen samples from 10 healthy roosters were subjected to four different treatments. For the treatments, 0.2 ml fresh semen with a sperm concentration of 5.2X109 cell/ml was mixed with T0 (no diluent), T1 (0.34 ml coconut water and 6 μl fructose), T2 (0.274 ml coconut water, 0.12 ml egg yolk, and 6 μl fructose), and T3 (0.34 ml egg yolk and 6 μl fructose) solutions. Each treated solution was stored at 5°C and evaluated both macroscopically and microscopically. Macroscopically, semen volume, pH, and sperm concentration were evaluated. The microscopic sperm characteristics examined included total motility (i.e., rapid, medium, or slow), progressive and non-progressive motility, viability, and spermatozoa abnormalities noted at different storage times. The results showed that spermatozoa motility was under 40%. Results: The results indicated that sperm viability significantly affected (p<0.05). The highest mean value of sperm viability on day 7 of storage was found after treatment with the T2 solution (46.100±0.5677%). Similarly, spermatozoa abnormalities were significantly lower after treatment with the T2 solution (6.680±1.702%). Conclusion: The addition of a diluent containing coconut water, egg yolk, and fructose helped in the better preservation spermatozoa motility, as well as viability for up to 7 days when the semen samples were stored at 5°C.

Chicken egg yolk antibodies (IgY)-based antivenom for neutralization of snake venoms: a review

Toxin Reviews ◽  
2021 ◽  
pp. 1-12
Author(s):  
Ankit Choraria ◽  
Rajeswari Somasundaram ◽  
S. Janani ◽  
Selvakumar Rajendran ◽  
Naoual Oukkache ◽  
...  
Keyword(s):  
Egg Yolk ◽  
Snake Venoms ◽  
Chicken Egg ◽  
Egg Yolk Antibodies ◽  
Chicken Egg Yolk

Development of viper-venom antibodies in chicken egg yolk and assay of their antigen binding capacity

Toxicon ◽  
2002 ◽  
Vol 40 (7) ◽  
pp. 857-861 ◽  
Author(s):  
C Maya Devi ◽  
M Vasantha Bai ◽  
L.K Krishnan
Keyword(s):  
Binding Capacity ◽  
Egg Yolk ◽  
Antigen Binding ◽  
Chicken Egg ◽  
Chicken Egg Yolk

Isolation of Immunoglobulin from Chicken Egg Yolk using Single-Stage Ultrafiltration with 100-kDa Regenerated Cellulose Membranes

2011 ◽  
Vol 7 (1) ◽  
Author(s):  
Jianguo Liu ◽  
Juan Yang ◽  
Hai Xu ◽  
Hu Zhu ◽  
Jianbo Qu ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Egg Yolk ◽  
Single Stage ◽  
Regenerated Cellulose ◽  
Cellulose Membrane ◽  
Permeate Flux ◽  
Operational Parameters ◽  
Solution Ph ◽  
Chicken Egg ◽  
Chicken Egg Yolk

The aim of this work is to develop a membrane-based cost-effective process for the rapid isolation of immunoglobulin from chicken egg yolk. It was found that a single-stage ultrafiltration using a 100 kDa molecular weight cut-off regenerated cellulose membrane could be employed to isolate immunoglobulin from the crude feedstock. The effects of operational parameters (solution pH, ionic strength, stirring speed and permeate flux) on the transmission of immunoglobulin and the presence of impurity protein with molecular weight close to immunoglobulin were quantified using the parameter scanning ultrafiltration technique. Under optimized conditions, the purity of immunoglobulin obtained was about 85 percent after the single-stage ultrafiltration process, and the recovery of immunoglobulin from the feedstock was 91 percent.

scholarly journals A simple method for isolating chicken egg yolk immunoglobulin using effective delipidation solution and ammonium sulfate

2015 ◽  
Vol 94 (1) ◽  
pp. 104-110 ◽  
Author(s):  
Chenyao Tong ◽  
Fang Geng ◽  
Zhenjiao He ◽  
Zhaoxia Cai ◽  
Meihu Ma
Keyword(s):  
Ammonium Sulfate ◽  
Egg Yolk ◽  
Simple Method ◽  
Chicken Egg ◽  
Egg Yolk Immunoglobulin ◽  
Chicken Egg Yolk

scholarly journals COMPARISON OF ANTIVENOM POTENTIAL OF CHICKEN EGG YOLK ANTIBODIES GENERATED AGAINST BENTONITE AND ADJUVENT COATED VENOMES OF COMMON POISONOUS SNAKE IN INDIA

1970 ◽  
Vol 7 (1) ◽  
pp. 259-267 ◽  
Author(s):  
S. Meenatchisundaram ◽  
R. Selvakumaran ◽  
G. Parameswari ◽  
A. Michael
Keyword(s):  
Room Temperature ◽  
Deae Cellulose ◽  
Egg Yolk ◽  
Precipitation Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Snake Venoms ◽  
Chicken Egg ◽  
Ammonium Sulphate Precipitation ◽  
Egg Yolk Antibodies ◽  
Chicken Egg Yolk

Antivenom antibodies were generated in white leghorn chicken against bentonite and adjuvant coated venoms of Common Indian Poisonous Snakes (Cobra, Krait, Russell's viper and Saw Scaled viper).The antivenom from immunized chicken egg yolk were purified by polyethylene glycol (PEG) and ammonium sulphate precipitation method and further purified by DEAE cellulose ion exchange column chromatography and concentrated by polyvinyl pyrolidone powder at room temperature. The titer of antibodies was estimated using Enzyme Linked Immunosorbent Assay (ELISA).The chickens immunized with Freund's complete adjuvant showed slightly higher titre when compared to bentonite. Inhibition of lethal, edema, haemorrhagic, procoagulant and phospholipase A2 and fibrinolytic activities of snake venoms were determined. The chicken egg yolk antivenom was effective in neutralization of these toxic and enzymatic activities of venom. The median effective dose (ED50) of chicken egg yolk antibodies raised against adjuvant coated venoms showed effective neutralizing venom toxicity when compared to the antibodies raised using bentonite coated venoms.

scholarly journals EVALUATION OF CULTURE SUBSTRATE FOR THE ISOLATION AND CULTIVATION OF LEGIONELLA WITH THE USE OF THE STRAINS ISOLATED DURING THE XXII OLYMPIC GAMES-2014 IN SOCHI

2017 ◽  
pp. 49-50
Author(s):  
N.V. Zharinova ◽  
E.B. Zhilchenko ◽  
L.S. Katunina ◽  
O.A. Konyaeva ◽  
N.S. Serdyuk ◽  
...  
Keyword(s):  
Experimental Evaluation ◽  
Olympic Games ◽  
Egg Yolk ◽  
Enzymatic Hydrolysate ◽  
Long Period ◽  
Chicken Egg ◽  
Environmental Objects ◽  
Culture Substrate ◽  
Chicken Egg Yolk ◽  
And Storage

The article presents the results of experimental evaluation of the effectiveness of new culture substrate developed for the isolation and cultivation of Legionella, the strains isolated from environmental objects in the resort city of Sochi before and during XXII Olympic games. Dense nutrient substrate prepared on the basis of enzymatic hydrolysate of chicken egg yolk and enzymatic hydrolysate of the lung of the pig with additional stimulants allowed saving number of colonies in the earlier stages of the study and storage of the isolated strains during long period of time compared with domestic commercial nutrient substrate.

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