scholarly journals Selective cyclooxygenase-2 inhibition prevents bone resorption

2005 ◽  
Vol 19 (1) ◽  
pp. 36-40 ◽  
Author(s):  
Carlos Augusto Nassar ◽  
Patrícia Oehlmeyer Nassar ◽  
Patrícia Maria Nassar ◽  
Luis Carlos Spolidorio
Keyword(s):  
Bone Loss ◽  
Alveolar Bone ◽  
Experimental Period ◽  
Root Surface ◽  
Negative Control ◽  
Alveolar Bone Loss ◽  
Cyclooxygenase 2 ◽  
The Other ◽  
Cox 2 ◽  
Experimentally Induced

The aim of the present work was to evaluate the effect of a selective cyclooxygenase-2 (COX-2) inhibitor (meloxicam) on the alveolar bone loss progression in experimentally induced periodontitis. Forty (40) Wistar rats were separated into 8 experimental groups (n = 5). Cotton ligatures were placed at the gingival margin level of the lower right first molars of some rats. Four groups were treated for 5 or 15 days with an oral dose of 15 mg/kg of body weight/day of the selective COX-2 inhibitor. The other groups were used as positive control (sham) or negative control in each experimental period. Standardized digital radiographs were taken after sacrifice at 5 and 15 days to measure the amount of bone loss at the mesial root surface of the first molar tooth in each rat. The treatment with meloxicam did not induce weight alteration or other visible systemic manifestations. One way analysis of variance (ANOVA) indicated that groups treated with meloxicam, after 5 days, had significantly less alveolar bone loss (p < 0.05) when compared with control groups. On the other hand, no significant differences in bone loss were observed after 15 days of treatment with meloxicam. These data provide evidence that systemic therapy with meloxicam can modify the progression of experimentally induced periodontitis in rats during the initial experimental period.

scholarly journals The effects of morus nigra on the alveolar bone loss in experimentally-induced periodontitis

2019 ◽  
pp. 99-105 ◽  
Author(s):  
Tuba Talo Yildirim ◽  
Gonca Ozan ◽  
Serkan Dundar ◽  
Alihan Bozoglan ◽  
Tahir Karaman ◽  
...  
Keyword(s):  
Bone Loss ◽  
Alveolar Bone ◽  
Alveolar Bone Loss ◽  
Morus Nigra ◽  
Experimentally Induced

Effect of Antimicrobial Agents on Root Surface Caries, Alveolar Bone Loss, and Microflora in Rice Rats

1986 ◽  
Vol 65 (5) ◽  
pp. 695-697 ◽  
Author(s):  
A. Toth ◽  
F.M. Beck ◽  
E.X. Beck ◽  
N. Flaxman ◽  
S. Rosen
Keyword(s):  
Bone Loss ◽  
Antimicrobial Agents ◽  
Alveolar Bone ◽  
Root Surface ◽  
Alveolar Bone Loss ◽  
Root Surface Caries

scholarly journals CpG immunostimulatory oligodeoxynucleotide 1826 as a novel nasal ODN adjuvant enhanced the protective efficacy of the periodontitis gene vaccine in a periodontitis model in SD rats

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Guohui Bai ◽  
Hang Yu ◽  
Xiaoyan Guan ◽  
Fengjiao Zeng ◽  
Xia Liu ◽  
...  
Keyword(s):  
Bone Loss ◽  
Morphometric Analysis ◽  
Alveolar Bone ◽  
Alveolar Bone Loss ◽  
Secretory Iga ◽  
Nasal Administration ◽  
Cpg Odn ◽  
Gene Vaccine ◽  
Sd Rats ◽  
Cox 2

Abstract Background We previously demonstrated that nasal administration of periodontitis gene vaccine (pVAX1-HA2-fimA) or pVAX1-HA2-fimA plus IL-15 as adjuvant provoked protective immunity in the periodontal tissue of SD rats. This study evaluated the immune effect of pVAX1-HA2-fimA plus CpG-ODN 1826 as an adjuvant in the SD rat periodontitis models to improve the efficacy of the previously used vaccine. Methods Periodontitis was induced in maxillary second molars in SD rats receiving a ligature and infected with Porphyromonas gingivalis. Forty-two SD rats were randomly assigned to six groups: A, control without P. gingivalis; B, P. gingivalis with saline; C, P. gingivalis with pVAX1; D, P. gingivalis with pVAX1-HA2-fimA; E, P. gingivalis with pVAX1-HA2-fimA/IL-15; F, P. gingivalis with pVAX1-HA2-fimA+CpG ODN 1826 (30 µg). The levels of FimA-specific and HA2-specific secretory IgA antibodies in the saliva of rats were measured by ELISA. The levels of COX-2 and RANKL were detected by immunohistochemical assay. Morphometric analysis was used to evaluate alveolar bone loss. Major organs were observed by HE staining. Results 30 μg could be the optimal immunization dose for CpG-ODN 1826 and the levels of SIgA antibody were consistently higher in the pVAX1-HA2-fimA+CpG-ODN 1826 (30 µg) group than in the other groups during weeks 1–8 (P < 0.05, except week 1 or 2). Morphometric analysis demonstrated that pVAX1-HA2-fimA+CpG-ODN 1826 (30 µg) significantly reduced alveolar bone loss in ligated maxillary molars in group F compared with groups B–E (P < 0.05). Immunohistochemical assays revealed that the levels of COX-2 and RANKL were significantly lower in group F compared with groups B–E (P < 0.05). HE staining results of the major organs indicated that pVAX1-HA2-fimA with or without CpG-ODN 1826 was not toxic for in vivo use. Conclusions These results indicated that CpG-ODN 1826 (30 µg) could be used as an effective and safe mucosal adjuvant for pVAX1-HA2-fimA in SD rats since it could elicit mucosal SIgA responses and modulate COX-2 and RANKL production during weeks 1–8, thereby inhibiting inflammation and decreasing bone loss.

scholarly journals Protective Effect of Resveratrol against the Alveolar Bone Loss in Rats with Experimental Periodontitis and Acts Positively on the IL-17

2021 ◽  
pp. 162-173
Author(s):  
JordanaHeidemann Pandini ◽  
Lais Fernanda Pasqualotto ◽  
Pedro Henrique de Carli Rodrigues ◽  
João Paulo Gonçalves De Paivaa ◽  
Patricia Oehlmeyer Nassar ◽  
...  
Keyword(s):  
Bone Loss ◽  
Protective Effect ◽  
Alveolar Bone ◽  
Experimental Period ◽  
Alveolar Bone Loss ◽  
Control Group ◽  
Interleukin 17 ◽  
Periodontal Tissues ◽  
Male Wistar Rats ◽  
Experimental Periodontitis

The resveratrol is a polyphenol known for its health benefits, which includes the ability to interfere in the osteoblastogenesis, which may foster adverse immunomodulators effects in the host response to periodontal disease. In the present study we evaluated the appearance of periodontal tissues of rats with experimentally induced periodontitis, by using resveratrol. Twenty-four male Wistar rats were used, in which half of the animals received a ligature around the first lower molars, then forming the groups with experimental periodontitis. Next, four groups were created: 1) Control Group (CON); 2) The Ligature Group (LIG); 3) Group Resveratrol (RSV); 4) Ligature-Resveratrol Group (LIG-RSV). The animals of the Resveratrol groups were daily dosed with 10 mg/kg of body weight of polyphenol orally, during four weeks. After 105 days of experimental period, euthanasia was performed. The results showed a significantly lower alveolar bone loss (p<0.05) in animals that received resveratrol, and still, the polyphenol was able to reduce concentration of interleukin 17 (IL-17) in the groups dosed with it. Our conclusion is that dosing rats with experimental periodontitis with resveratrol could cause a protective effect on the alveolar bone loss, in addition to act positively on the IL-17.

scholarly journals Effects of Colocasia antiquorum var. Esculenta Extract In Vitro and In Vivo against Periodontal Disease

Medicina ◽  
2021 ◽  
Vol 57 (10) ◽  
pp. 1054
Author(s):  
Seong-Hee Moon ◽  
Seong-Jin Shin ◽  
Hyun-Jin Tae ◽  
Seung-Han Oh ◽  
Ji-Myung Bae
Keyword(s):  
Bone Loss ◽  
Periodontal Disease ◽  
Pathogenic Bacteria ◽  
Alveolar Bone ◽  
Negative Control ◽  
Alveolar Bone Loss ◽  
Oral Microbiome ◽  
Control Group

Background and Objectives: Periodontal disease is a chronic inflammatory disease in which gradual destruction of tissues around teeth is caused by plaque formed by pathogenic bacteria. The purpose of this study was to evaluate the potential of 75% ethanol extract of Colocasia antiquorum var. esculenta (CA) as a prophylactic and improvement agent for periodontal disease in vitro and in vivo. Materials and Methods: The antimicrobial efficacy of CA against Porphyromonas gingivalis (P. gingivalis, ATCC 33277) was evaluated using minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) test, and cytotoxicity was confirmed by CCK-8 assay. For the in vivo study, P. gingivalis was applied by oral gavage to BALB/c mice. Forty-two days after the first inoculation of P. gingivalis, intraoral swabs were taken for microbiome analysis, and the mice were sacrificed to evaluate the alveolar bone loss. Results: The MIC of CA against P. gingivalis was 31.3 μg/mL, the MBC was 62.5 μg/mL, with no cytotoxicity. The diversity of the oral microbiome decreased in the positive control group, while those of the VA (varnish) and VCA (varnish added with CA) groups increased as much as in the negative control group, although the alveolar bone loss was not induced in the mouse model. Conclusions: CA showed antibacterial effects in vitro, and the VA and VCA groups exhibited increased diversity in the oral microbiome, suggesting that CA has potential for improving periodontal disease.

COX-2 Inhibition Decreases VEGF Expression and Alveolar Bone Loss During the Progression of Experimental Periodontitis in Rats

2008 ◽  
Vol 79 (6) ◽  
pp. 1062-1069 ◽  
Author(s):  
Thais M. Oliveira ◽  
Vivien T. Sakai ◽  
Maria Aparecida A.M. Machado ◽  
Thiago J. Dionísio ◽  
Tania Mary Cestari ◽  
...  
Keyword(s):  
Bone Loss ◽  
Alveolar Bone ◽  
Alveolar Bone Loss ◽  
Vegf Expression ◽  
Experimental Periodontitis ◽  
Cox 2

Selective Cyclooxygenase-2 Inhibition Prevents Alveolar Bone Loss in Experimental Periodontitis in Rats

2000 ◽  
Vol 71 (6) ◽  
pp. 1009-1014 ◽  
Author(s):  
Mirna M. Bezerra ◽  
Vilma de Lima ◽  
Veruska B.M. Alencar ◽  
Ivana B. Vieira ◽  
Gerly Anne C. Brito ◽  
...  
Keyword(s):  
Bone Loss ◽  
Alveolar Bone ◽  
Alveolar Bone Loss ◽  
Cyclooxygenase 2 ◽  
Experimental Periodontitis

scholarly journals Evaluation of bone loss due to primary occlusal trauma in two experimental models of occlusal overload

2016 ◽  
Vol 45 (4) ◽  
pp. 183-188 ◽  
Author(s):  
Ana Cristina Távora de Albuquerque LOPES ◽  
Mirela Anne Quartaroli TÉO ◽  
Mônica Grazieli CORRÊA ◽  
Bella Luna Colombini ISHIKIRIAMA ◽  
Mirella Lindoso Gomes CAMPOS
Keyword(s):  
Bone Loss ◽  
Alveolar Bone ◽  
Negative Control ◽  
Experimental Models ◽  
Alveolar Bone Loss ◽  
Normal Height ◽  
Orthodontic Wire ◽  
Wire Segment ◽  
The Impact ◽  
Lower Molar

Abstract Introduction Primary occlusal trauma (OT) is an injury of the periodontium with normal height as a result of occlusal forces which exceed their adaptive capacity. Objective To evaluate, histometrically, the alveolar bone loss in the furcation region of rats experimentally submitted to 2 models of occlusal overload. Material and method 45 animals randomly divided into 3 groups: Occlusal Interference (OI, n = 15) - fixing an orthodontic wire segment on the occlusal surface of the first lower molar; Occlusal Overload (OO, n = 15) - wearing of the cusps of the lower contralateral molars, the second and third molars next to the first molar that had its dimensions maintained; Negative Control (NC, n = 15) - evaluation of the initial dimensions of the periodontal ligament (PL). Five animals / group were sacrificed after 14, 21 and 28 days. Result Intergroup evaluation showed significant bone loss in OI (p<0.001) and OO (p<0.01) compared to NC. OI had significantly higher bone loss compared to OO at 14 (p<0.01), 21 (p <0.01) and 28 days (p<0.01). The intragroup evaluation showed no significant influence of time on bone loss in OI and OO, regardless of the technique used (p>0.05). The thickness of the PL remained stable in NC (p>0.05). Conclusion OI and OO were effective in the experimental reproduction of OT, and OI promoted greater alveolar bone loss compared to OO, showing that the impact of occlusal overload in OI increased the extent of the OT injury.

scholarly journals Effects of Selective Versus Non-Selective COX-2 Inhibition on Experimental Periodontitis

2019 ◽  
Vol 30 (2) ◽  
pp. 133-138 ◽  
Author(s):  
Marcella Goetz Moro ◽  
Marilia Dantas dos Santos Oliveira ◽  
Leticia Rodrigues de Oliveira ◽  
Simone Aparecida Teixeira ◽  
Marcelo Nicolas Muscará ◽  
...  
Keyword(s):  
Bone Loss ◽  
Periodontal Disease ◽  
Periodontal Ligament ◽  
Alveolar Bone ◽  
Alveolar Bone Loss ◽  
Cervical Region ◽  
Radiographic Examination ◽  
Tissue Samples ◽  
Disease Induction ◽  
Cox 2

Abstract In the present study we compared the effects of the selective COX-2 inhibitor etoricoxib with those of the classical non-selective NSAID diclofenac on the inflammatory process and alveolar bone loss in an experimental model of periodontitis in rats. Ninety male Holtzman rats (250 g) were randomly sorted into four experimental groups: Sham+CMC and Ligature+CMC (control) groups which received 0.5% carboxymethylcellulose sodium (CMC) solution; Ligature+Diclofenac and Ligature+Etoricoxib groups which received Potassium Diclofenac and Etoricoxib, respectively, suspended in 0.5% CMC (10 mg/kg/day). At 7, 14 and 21 days after placing ligatures in the cervical region of both the lower right and left first molars, the animals were euthanized. At the end of each period, the mandibles were collected for radiographic examination of alveolar bone loss. In addition, alveolar bone and periodontal ligament tissue samples were collected for COX-2 expression analysis and gingival tissues were collected for measurement of PGE2 contents. Animals with ligature-induced periodontal disease showed significant increased COX-2 gene expression at days 7, 14 and 21 (p<0.05) on alveolar bone and periodontal ligament. However, both treatments resulted in significantly reduced alveolar bone loss when compared to the untreated Ligature group (p<0.05), with no statistical difference between Etoricoxib and Diclofenac Potassium groups. This study shows that both drugs were able to reduce alveolar bone loss after periodontal disease induction.

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