scholarly journals Factors associated to Theileria equi in equids of two microregions from Rio de Janeiro, Brazil

2011 ◽  
Vol 20 (3) ◽  
pp. 235-241 ◽  
Author(s):  
Tiago Marques dos Santos ◽  
Erica Cristina Rocha Roier ◽  
Huarrisson Azevedo Santos ◽  
Marcus Sandes Pires ◽  
Joice Aparecida Rezende Vilela ◽  
...  
Keyword(s):  
Rio De Janeiro ◽  
Fluorescent Antibody ◽  
Prevalence Ratio ◽  
Antibody Test ◽  
Geographic Area ◽  
Southeastern Brazil ◽  
Amblyomma Cajennense ◽  
Serum Samples ◽  
Theileria Equi ◽  
Factors Associated

Serum samples from 714 equids of Itaguaí and Serrana microregions, Rio de Janeiro, southeastern Brazil, were examined by indirect fluorescent antibody test (titer 1:80) for Theileria equi. The prevalence in the microregions and factors associated with seropositivity were evaluated and the prevalence ratio (PR) calculated. The overall prevalence of T. equi infection was 81.09% (n = 579), with higher prevalence (p < 0.05) in the Itaguaí (85.43%) when compared to Serrana microregion (76.92%). The geographic area, altitude, farming condition and area of origin of equids were associated (p < 0.05) with seropositivity for T. equi. Equids reared in the Itaguaí microregion (PR = 1.11, p = 0.003) and at altitudes below 500 m (PR = 1.10; p = 0,014) were more likely to be seropositive for T. equi. Furthermore, when equids were born in the farm (PR = 1.10, p = 0.008) and reared with poor farming conditions (PR = 1.13, p = 0.018) they were more likely to be exposed to T. equi. The main ticks found on equids were Amblyomma cajennense and Dermacentor (Anocentor) nitens. The microregions studied are endemic areas for equine theileriosis and there exists enzootic stability for T. equi. Only factors related to the collection area of serum samples influenced the seropositivity of equids for T. equi in that region.

scholarly journals Seroepidemiological aspects ofLeishmania spp. in dogs in the Itaguai micro-region, Rio de Janeiro, Brazil

2013 ◽  
Vol 22 (1) ◽  
pp. 39-45 ◽  
Author(s):  
Claudia Bezerra da Silva ◽  
Joice Aparecida Rezende Vilela ◽  
Marcus Sandes Pires ◽  
Huarrisson Azevedo Santos ◽  
Aline Falqueto ◽  
...  
Keyword(s):  
Rural Areas ◽  
Rio De Janeiro ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Canine Leishmaniasis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Factors Associated ◽  
Definition Of ◽  
Forest Streams

This study evaluated factors associated with the frequency ofLeishmania spp. antibodies in dogs residing in the Itaguai micro-region, State of Rio de Janeiro, Brazil. Blood samples were collected from 524 dogs. The serum samples were submitted to indirect fluorescent antibody test (IFAT) and enzyme-linked immunosorbent assay (ELISA) forLeishmania spp. The frequency of seropositive dogs was 28.24% (n = 148) in the micro-region, and among the three municipalities within that region, the highest frequency (p < 0.05) was observed in Seropedica (59.46%), followed by Itaguai (29.05%) and Mangaratiba (11.49%). Regarding factors associated with the host, mongrel dogs and those over the age of two presented higher frequency of antibodies to Leishmaniaspp. (p < 0.05). Concerning factors related to the environment and habits of the animal, dogs residing in rural areas (FR = 1.67, p = 0.0002), living outside the residence (FR = 1.42, p = 0.0197), with access to forest, streams and pastures (FR = 2.81, p = 0.0007), remaining loose (FR = 1.66, p = 0.0073), and those that had no shelter (FR = 2.16, p < 0.0001) were more likely to be seropositive. Canine leishmaniasis is a disease with high occurrence in the Itaguai micro-region, and aspects such as the definition of breed, age, habits and care by owners showed significant association in this micro-region.

scholarly journals Factors associated with the distribution of natural Toxoplasma gondii infection among equids in Northeastern Brazil

2019 ◽  
Vol 28 (2) ◽  
pp. 283-290 ◽  
Author(s):  
Alexandre Dias Munhoz ◽  
Monia Andrade Souza ◽  
Sonia Carmen Lopo Costa ◽  
Jéssica de Souza Freitas ◽  
Aísla Nascimento da Silva ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Rural Areas ◽  
Urban Areas ◽  
Linear Models ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Northeastern Brazil ◽  
Domestic Cats ◽  
Serum Samples ◽  
Factors Associated

Abstract The aim of this study was to determine the frequency and factors associated with Toxoplasma gondii in naturally infected equids in northeastern Brazil. Serum samples from 569 equids (528 horses, 8 mules and 33 donkeys) were subjected to the indirect fluorescent antibody test. Generalized linear models were used to evaluate associated factors. Among the 569 animals sampled, 118 (30.6%) living in rural areas and 14 (26.42%) in urban areas were seropositive (p>0.05). Seropositive animals were observed on 95% of the farms and in all the municipalities. Donkeys/mules as the host, presence of domestic cats and rats on the farm, ingestion of lagoon water and goat rearing remained in the final model as factors associated with infection. Preventive measures such as avoiding the presence of domestic cats close to rearing areas, pastures and sources of water for the animals should be adopted. The wide-ranging distribution of positive animals also indicated that infection in other domestic animals and in humans, through the contaminated environment, was possible. It should be highlighted that there was the possibility that donkeys and mules would continue to have detectable titers for longer, thus explaining the prevalence found. Further studies are needed to confirm this possibility.

scholarly journals Seroepidemiology ofLeishmaniaspp. in equids from Uberlândia, Minas Gerais, Brazil

2017 ◽  
Vol 47 (5) ◽  
Author(s):  
Patrícia Magalhães de Oliveira ◽  
Felipe Garcia ◽  
Fernanda Evers ◽  
Vinícius de Morais Barbosa ◽  
Diego Camilo Mora Obando ◽  
...  
Keyword(s):  
Economic Activity ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Epidemiological Survey ◽  
Minas Gerais ◽  
Southeastern Brazil ◽  
Wild Animals ◽  
Serum Samples ◽  
Leishmania Spp

ABSTRACT: This study evaluated the occurrence of Leishmania spp. in equids from Uberlândia, Minas Gerais, Southeastern Brazil. Serum samples from 257 healthy equids of Uberlândia were assessed for the presence of antibodies against Leishmania spp. by using the indirect fluorescent-antibody test. Additionally, an epidemiological survey was done to evaluate the possible risk factors (species of equids, age, gender, economic activity, and contact with domestic and wild animals) associated with the presence of antibodies against Leishmania spp. The total seroprevalence against Leishmania spp. was 24.1% (62/257); representing seroprevalence levels of 22.7% (55/242) in horses, 50% (4/8) in mules, and 42.9% (3/7) in ponies from all regions evaluated. The species of equid was the only factor that contributed to an elevated seroprevalence of Leishmania spp.

scholarly journals Risk factor analysis associated with Neospora caninum in dairy cattle in Western Brazilian Amazon

2021 ◽  
Vol 30 (1) ◽  
Author(s):  
Poliana de Jesus Souza Venturoso ◽  
Osvaldo Juliatti Venturoso ◽  
Gisele Glomba Silva ◽  
Maerle Oliveira Maia ◽  
Rute Witter ◽  
...  
Keyword(s):  
Risk Factors ◽  
Dairy Cattle ◽  
Neospora Caninum ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Serum Samples ◽  
Present Survey ◽  
Caninum Infection ◽  
Factors Associated ◽  
And Control

Abstract Neospora caninum is considered to be one of the main causes of abortion among cattle. The present survey was conducted in the municipality of Rolim de Moura, Rondônia State, Brazil. A questionnaire that investigates the epidemiological aspects of neosporosis was used in the analysis of risk factors associated with the animal-level and herd-level prevalence in dairy cattle. A total of 416 bovine blood samples were collected from 30 farms, and N. caninum antibody levels were measured by Indirect Fluorescent Antibody Test (IFAT). Analysis of dairy cattle serum samples revealed the presence of anti-N. caninum antibodies to be 47.36% (n = 197). Risk factors associated with N. caninum infection were the management system and access locations of dogs. The results of the present survey indicated that infection of dairy cattle with N. caninum is widespread in the studied region of Western Amazon, which has implications for prevention and control of neosporosis in this region. Therefore, integrated control strategies and measures are recommended to prevent and control N. caninum infection in dairy cattle. In addition, direct contact between dairy cattle, dogs and wild animals, which can influence the epidemiology of neosporosis, should be investigated further.

scholarly journals Sarcocystis neurona, seroprevalence of antibodies in equines and research of oocystis in opossum in Ilhéus - Itabuna microregion, Bahia, Brazil

2021 ◽  
Vol 30 (2) ◽  
Author(s):  
Samantha Gusmão Pellizzoni ◽  
Sônia Carmen Lopo Costa ◽  
Raissa Barros Gracie Mery ◽  
Jonata Melo Barbieri ◽  
Alexandre Dias Munhoz ◽  
...  
Keyword(s):  
Protective Factor ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Serum Samples ◽  
Sarcocystis Neurona ◽  
Factors Associated ◽  
Sarcocystis Spp ◽  
Southern Bahia ◽  
Stool Samples

Abstract The aims of this study were to determine the seroprevalence of Sarcocystis neurona antibodies in equines in the Ilhéus-Itabuna microregion (BA), and identify possible factors associated with infection. The presence of sporocysts/oocysts of Sarcocystis spp. was also verified in Didelphis spp. A total of 669 serum samples were collected from equines in 56 properties located in 12 municipalities in the region. Indirect fluorescent antibody test was performed with slides containing merozoites of the S. neurona, using a cut-off titer of 1:80. Occurrence of 7.92% of anti-S. neurona antibodies was observed in the sampled equines. The purposes trade and work were significantly associated with the presence of antibodies (p<0.05), and being used for the purpose of work (21.6%) was considered a risk factor, while being used for the purpose of trade (3.6%) was a protective factor. A total of 25 Didelphis spp. was captured for research on sporocysts/oocysts in stool samples and intestinal scrapings, being all negative. Didelphis spp. were all negative for the presence of Sarcocystis spp. and this circumstance does not change the fact that seroprevalence of S. neurona has been observed in horses raised in the southern Bahia.

scholarly journals Serologic evidence of equine granulocytic anaplasmosis in horses from central West Brazil

2010 ◽  
Vol 19 (3) ◽  
pp. 135-140 ◽  
Author(s):  
Carlos Augusto Salvagni ◽  
Ana Sílvia Dagnone ◽  
Tiago Salles Gomes ◽  
Jozivaldo Silva Mota ◽  
Gisele Maria Andrade ◽  
...  
Keyword(s):  
Fluorescent Antibody ◽  
Antibody Test ◽  
Buffy Coat ◽  
Molecular Techniques ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Theileria Equi ◽  
Nested Polymerase Chain Reaction ◽  
Granulocytic Ehrlichiosis ◽  
Granulocytic Anaplasmosis

Ehrlichiosis is a zoonotic disease caused by gram-negative and intracellular obligatory bacterial organisms. Equine Granulocytic Anaplasmosis - EGA (formerly Equine Granulocytic Ehrlichiosis, EGE) is a seasonal disease, normally self-limited in horses. There are few reports in Brazil about this ehrlichial agent, as well as its natural vectors. Nowadays, veterinarians are considering the suspicion of EGA in horses with suggestive symptoms of ehrlichiosis and which do not respond to piroplasmosis treatment. The aim of the present study was to identify horses exposed to the agent A. phagocytophilum by serological and molecular techniques. Twenty equine blood and serum samples from the central West region of Brazil were evaluated by microscopic examination of buffy coat smear, enzyme-linked immunosorbent assay (ELISA), indirect fluorescent antibody test (IFAT) and nested polymerase chain reaction (nPCR). Additionally, the serodiagnosis of Theileria equi by IFA and ELISA were carried out, as well as molecular diagnosis by nPCR. Thirteen (65%) serum samples were positive for A. phagocytophilum by ELISA, but none of them were positive by buffy-coat smear examination or nPCR. Antibodies IgG anti-T. equi were detected in 18 (90%) and 17 (85%) horses by IFA and ELISA, respectively and the agent was detected in 9 (45%) animals by nPCR. Our data may be considered as important information to understanding the occurrence of EGA and equine piroplasmosis in central West Brazil.

scholarly journals Prevalence and risk factors associated to ovine toxoplasmosis in northeastern Brazil

2013 ◽  
Vol 22 (2) ◽  
pp. 230-234 ◽  
Author(s):  
Carlos Eduardo D'Alencar Mendonça ◽  
Sílvia Letícia Bomfim Barros ◽  
Vitor Andrade Accioly Guimarães ◽  
Antonio Sergio Ferraudo ◽  
Alexandre Dias Munhoz
Keyword(s):  
Risk Factors ◽  
Toxoplasma Gondii ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Control Measures ◽  
Northeastern Brazil ◽  
Serum Samples ◽  
Factors Associated ◽  
Deep Well ◽  
Toxoplasma Gondii Infection

In this study, we aimed to determine the prevalence and risk factors associated to Toxoplasma gondii infection in sheep from northeastern Brazil. A total of 932 ovine serum samples from 54 properties in 19 municipalities of the state of Sergipe were collected and assayed using indirect fluorescent antibody test. The assay used antibodies against Toxoplasma gondii (IFAT-IgG) with a cutoff point of 1:64. We observed that 28.22% (263/932) of the ovine samples were serum-reactive. In a logistic regression, factors such as consumption of water directly from the source, consumption of water from a deep well, and age below 12 months were associated with protection; whereas factors such as presence of cats on the property, presence of slatted floor, and use of exchanged or borrowed breeding males were associated with infection. The studied area can be considered endemic for toxoplasmosis, so it is necessary to adopt preventive and control measures because this zoonotic infection poses risks to public health.

scholarly journals Seroprevalence of Toxoplasma gondii infection in swine matrices in Nova Mutum and Diamantino, Mato Grosso, Brazil

2010 ◽  
Vol 19 (4) ◽  
pp. 254-255 ◽  
Author(s):  
Lívia Saab Muraro ◽  
João Garcia Caramori Júnior ◽  
Maria Regina Reis Amendoeira ◽  
Joyce Alves Pereira ◽  
João Xavier de Oliveira Filho ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
The State ◽  
Indirect Fluorescent Antibody ◽  
Risk Of Infection ◽  
Serum Samples ◽  
Mato Grosso ◽  
Toxoplasma Gondii Infection

This report aimed to assess the seroprevalence of Toxoplasma gondii infection in 708 swine matrices in Nova Mutum and Diamantino in the state of Mato Grosso, Central-West Brazil. Serum samples were examined by indirect fluorescent antibody test (IFAT). It was found a seroprevalence of 12.8%, considering titers >64. Therefore, the data reinforce the need for appropriate management of swine raising to minimize the risk of infection of pigs with T. gondii.

scholarly journals Risk factors for Toxoplasma gondii and Neospora caninum seropositivity in buffaloes in Paraiba State, Brazil

2015 ◽  
Vol 24 (4) ◽  
pp. 459-463 ◽  
Author(s):  
Arthur Willian de Lima Brasil ◽  
Roberta Nunes Parentoni ◽  
Thais Ferreira Feitosa ◽  
Camila de Sousa Bezerra ◽  
Vinicius Longo Ribeiro Vilela ◽  
...  
Keyword(s):  
Risk Factors ◽  
Toxoplasma Gondii ◽  
Odds Ratio ◽  
Indirect Fluorescent Antibody Test ◽  
Neospora Caninum ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Management Systems ◽  
Intensive Management ◽  
Factors Associated

Abstract The aims of this survey were to determine the frequency of anti-Toxoplasma gondii and anti-Neospora caninum antibodies and to identify the risk factors associated with seropositivity among buffaloes in the state of Paraíba, Brazil. This survey included 136 buffaloes belonging to 14 herds. To detect anti-T. gondii and anti-N. caninum antibodies, the indirect fluorescent antibody test (IFAT) was used. Among the 136 samples analyzed, 17 (12.5%) were positive for anti-T. gondii antibodies with titers ranging from 64 to 1,024, and 26 (19.1%) for anti-N. caninum with titers from 200 to 1,600. Animals seropositive for both T. gondii and N. caninum were found in 10 of the 14 herds (71.4%). Semi-intensive management systems (odds ratio = 2.99) and presence of pigs (odds ratio = 4.33) were identified as risk factors for T. gondii and N. caninum, respectively. It can be suggested that T. gondii and N. caninum are widespread in buffaloes in Paraíba, and that additional surveys are needed in order to ascertain the importance of these agents for this species and for pigs, and the influence of the farming type on occurrences of seropositive animals.

scholarly journals Prevalence of Toxoplasma gondii indirect fluorescent antibodies in naturally- and experimentally-infected chickens (Gallus domesticus) in Thailand

2009 ◽  
Vol 54 (3) ◽  
Author(s):  
Kamlang Chumpolbanchorn ◽  
Pacharaporn Anankeatikul ◽  
Wantanee Ratanasak ◽  
Jitbanjong Wiengcharoen ◽  
R. Andrew Thompson ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Health Risks ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Gallus Domesticus ◽  
Indirect Fluorescent Antibody ◽  
Serum Samples ◽  
Public Health Risks ◽  
Potential Public Health

AbstractToxoplasma gondii infections in free-range (FR) chickens (Gallus domesticus) are potential public health risks. Antibodies for T. gondii were found in 194 out of 303 serum samples (64.03%) from FR chickens in Thailand tested by the indirect fluorescent antibody test (IFAT, 1:16). To verify the validity of serologic data in this survey, sera from chickens experimentally infected with the RH strain of T. gondii were tested by the IFAT. Antibodies against T. gondii were detected as early as 7 days p.i., peaked at 2 weeks, and then declined by 10 weeks p.i.

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