scholarly journals Physiological Basis for Metamifop Selectivity on Bermudagrass (Cynodon dactylon) and Goosegrass (Eleusine indica) in Cool-Season Turfgrasses

Weed Science ◽  
2016 ◽  
Vol 64 (1) ◽  
pp. 12-24 ◽  
Author(s):  
Patrick E. McCullough ◽  
Jialin Yu ◽  
Mark A. Czarnota ◽  
Paul L. Raymer

Bermudagrass and goosegrass are problematic weeds with limited herbicides available for POST control in creeping bentgrass. Metamifop effectively controls these weeds with greater selectivity in cool-season grasses than other ACCase inhibitors. The objectives of this research were to determine the physiological basis for metamifop selectivity in turfgrasses. In greenhouse experiments, metamifop rate required to reduce shoot biomass 50% from the nontreated (GR50) at 4 wk after treatment was > 6,400, 2,166, and 53 g ai ha−1for creeping bentgrass, Kentucky bluegrass, and goosegrass, respectively. The GR50for bermudagrass treated with diclofop-methyl or metamifop was 2,850 and 60 g ha−1, respectively. In laboratory experiments, peak absorption of14C-metamifop was reached at 48, 72, and 96 h after treatment (HAT) for goosegrass, creeping bentgrass and Kentucky bluegrass, respectively. Grasses translocated < 10% of the absorbed radioactivity out of the treated leaf at 96 HAT, but creeping bentgrass translocated three times more radioactivity than goosegrass and Kentucky bluegrass. Creeping bentgrass, Kentucky bluegrass, and goosegrass metabolized 16, 14, and 25% of14C-metamifop after 96 h, respectively. Goosegrass had around two times greater levels of a metabolite at retention factor 0.45 than creeping bentgrass and Kentucky bluegrass. The concentration of metamifop required to inhibit isolated ACCase enzymes 50% from the nontreated (I50) measured > 100, > 100, and 38 μM for creeping bentgrass, Kentucky bluegrass, and goosegrass, respectively. In other experiments, foliar absorption of14C-metamifop in bermudagrass was similar to14C-diclofop-methyl. Bermudagrass metabolized 23 and 60% of the absorbed14C-diclofop-methyl to diclofop acid and a polar conjugate after 96 h, respectively, but only 14% of14C-metamifop was metabolized. Isolated ACCase was equally susceptible to inhibition by diclofop acid and metamifop (I50= 0.7 μM), suggesting degradation rate is associated with bermudagrass tolerance levels to these herbicides. Overall, the physiological basis for metamifop selectivity in turfgrass is differential levels of target site inhibition.

2010 ◽  
Vol 20 (1) ◽  
pp. 179-185 ◽  
Author(s):  
Bernd Leinauer ◽  
Matteo Serena ◽  
Devesh Singh

A field experiment was conducted at New Mexico State University to investigate the effect of seeding rates and ZEBA polymer [starch-g-poly (2-propenamide-co-propenoic acid) potassium salt] seed coating on the germination and establishment of warm- and cool-season grasses, and cool-season blends and mixtures. Grasses were established at recommended and reduced (50% of recommended) seeding rates with coated and uncoated seeds under two irrigation regimes (98% and 56% reference evapotranspiration). With the exception of ‘Bengal’ creeping bentgrass (Agrostis stolonifera), the polymer coating did not improve germination of the turfgrasses tested 22 days after seeding (DAS). However, at the end of the establishment period (92 DAS), plots established with ‘Bengal’, Dunes Mix [mixture of ‘Hardtop’ hard fescue (Festuca longifolia), ‘Baron’ kentucky bluegrass (Poa pratensis), ‘Barok’ sheep fescue (Festuca ovina)], ‘Panama’ bermudagrass (Cynodon dactylon), and Turf Sense™ [mixture of ‘Baronie’ kentucky bluegrass, ‘Barlennium’ perennial ryegrass (Lolium perenne), and ‘Barcampsia’ tufted hairgrass (Deschampsia cespitosa)] achieved greater coverage (based on visual estimations) when coated seed was used compared with uncoated seed. Establishment was greater for ‘Bengal’, Dunes Mix, ‘Panama’, Turf Sense™, and Turf Saver™ [blend of ‘Barlexas II’, ‘Barrington’, and ‘Labarinth’ tall fescue (Festuca arundinacea)] when normal seeding rates were applied compared with reduced seeding rates. ‘Barleria’ crested hairgrass (Koeleria macrantha) plots did not establish, regardless of the treatments applied. Results showed that seed coating has the potential to improve establishment at recommended and reduced seeding rates and can compensate for less favorable conditions such as reduced irrigation, reduced seeding rate, or for a combination of both. At the end of the establishment period, not all grasses achieved coverage greater than 50%. Further research over a longer establishment period is needed to determine if coated seed can be beneficial in achieving full coverage.


Weed Science ◽  
2016 ◽  
Vol 64 (1) ◽  
pp. 33-41 ◽  
Author(s):  
Patrick E. McCullough ◽  
Jialin Yu ◽  
J. Scott McElroy ◽  
S. Chen ◽  
H. Zhang ◽  
...  

Acetolactate synthase (ALS) inhibitors are widely used for POST control of sedges in turfgrass. A suspected resistant (R) biotype of annual sedge was collected from a bermudagrass turf in Georgia with a history of exclusive use of halosulfuron. Research was conducted to evaluate the resistance level of this biotype to halosulfuron, efficacy of ALS-inhibiting herbicides and other mechanisms of action for control, and the molecular and physiological basis for resistance. In greenhouse experiments, the halosulfuron rate required to reduce shoot biomass 50% in comparison with the nontreated at 8 wk after treatment (WAT) were 8 and > 1,120 g ai ha−1for the S (susceptible) and R biotypes, respectively. Imazapic, sulfosulfuron, and trifloxysulfuron reduced biomass of the S biotype greater than 60% at 8 WAT, but biomass was reduced less than 20% for the R biotype. Glufosinate, glyphosate, MSMA, and sulfentrazone reduced shoot biomass of the R biotype by 93, 86, 97, and 45%, respectively. In laboratory experiments, the halosulfuron concentration required to inhibit ALS activity by 50% in excised leaf tissues was 5.8 and > 1,000 μM for the S and R biotypes, respectively. Gene sequencing of the R biotype revealed a Pro-197-Ser substitution that confers resistance to ALS inhibitors. This is the first report of ALS-inhibitor resistance in annual sedge and herbicide resistance in a sedge species from a turfgrass system.


2012 ◽  
Vol 26 (3) ◽  
pp. 582-586 ◽  
Author(s):  
Jeffrey F. Derr

Broadleaf weeds are common and troublesome pests in cool-season turfgrass species such as tall fescue, Kentucky bluegrass, perennial ryegrass, and creeping bentgrass. Broadleaf weeds are primarily managed in these grasses through POST applications of growth regulator herbicides in the phenoxy, benzoic acid, and pyridine chemical classes. There are disadvantages to use of these chemicals, including nontarget plant damage and limited residual control. Certain annual broadleaf weeds can be controlled through application of isoxaben or a PRE crabgrass herbicide, but these herbicides do not control emerged broadleaf weeds. There are advantages to use of sulfonylurea herbicides, including PRE and POST control of annual and perennial weeds, a different mode of action, and these herbicides have low vapor pressure, reducing the potential for offsite movement. There are disadvantages to the use of sulfonylurea herbicides, including limited spectrum of broadleaf weed species controlled and limited tolerance in cool-season turfgrass species. The primary sulfonylurea herbicides used in cool-season turfgrass are chlorsulfuron, halosulfuron, metsulfuron, and sulfosulfuron. There have been specialized uses for primisulfuron and tribenuron-methyl.


2016 ◽  
Vol 26 (4) ◽  
pp. 394-398 ◽  
Author(s):  
Tyler Cooper ◽  
Leslie L. Beck ◽  
Chase M. Straw ◽  
Gerald M. Henry

Metamifop is a postemergence aryloxyphenoxypropionic acid herbicide used for the control of annual and perennial grass weeds in cereal crops and rice (Oryza sativa L.). Previous research observed creeping bentgrass (Agrostis stolonifera L.) tolerance to applications of metamifop, suggesting utilization for the removal of encroaching bermudagrass (Cynodon Rich.) from creeping bentgrass putting greens with little to no phytotoxicity. Therefore, the objective of our research was to evaluate the efficacy of metamifop for common bermudagrass [Cynodon dactylon (L.) Pers.] control in a greenhouse environment. Experiments were conducted at the Plant and Soil Science greenhouse facility at Texas Tech University in Lubbock in 2011 and 2012. ‘Riviera’ and ‘Savannah’ common bermudagrass were seeded at 218 lb/acre into 4-inch square pots containing a soilless potting media on 26 Aug. 2011 and 14 Nov. 2011. Pots were allowed to mature in the greenhouse over a 3-month period where they were maintained at a height of 0.25 inches. Herbicide treatments were applied on 1 Dec. 2011 and 8 Feb. 2012 and consisted of metamifop at 0.18, 0.27, 0.36, or 0.45 lb/acre. A sequential application of each treatment was made on 22 Dec. 2011 and 29 Feb. 2012. A nontreated control was included for comparison. Clipping ceased after initial herbicide treatment and pots produced biomass for 3 weeks. Biomass above 0.25 inch was removed from each pot, dried, and weighed. This procedure was conducted again 3 weeks after sequential treatments. The rate of metamifop required to reduce bermudagrass growth 50% (GR50) was calculated 3 and 6 weeks after initial treatment (WAIT). Visual ratings of percent bermudagrass control were recorded weekly on a scale of 0% (no control) to 100% (completely dead bermudagrass). As metamifop rate increased, bermudagrass biomass decreased. The calculated GR50 at 3 WAIT for ‘Savannah’ and ‘Riviera’ was 0.19 and 0.14 lb/acre, respectively. Nontreated control pots exhibited 0% control and produced 0.59 to 0.83 g of biomass at 3 WAIT, regardless of cultivar. Metamifop at 0.27 to 0.45 lb/acre exhibited 96% to 100% bermudagrass control at 3 WAIT, regardless of cultivar. Bermudagrass subjected to those same treatments only produced 0.01 to 0.03 g of biomass at 3 WAIT, regardless of cultivar. The 0.18-lb/acre rate of metamifop exhibited only 9% control of ‘Savannah’ bermudagrass with 0.72 g of biomass collected, while ‘Riviera’ was controlled 41% with 0.38 g of biomass collected. The calculated GR50 at 6 WAIT for ‘Savannah’ and ‘Riviera’ was 0.13 and 0.14 lb/acre, respectively. Sequential applications of metamifop at 0.27 to 0.45 lb/acre completely controlled bermudagrass (100%) at 6 WAIT, while a sequential application at 0.18 lb/acre only controlled bermudagrass 8% to 19% at 6 WAIT, regardless of cultivar. Bermudagrass subjected to 0.18 lb/acre exhibited 0.48 to 0.56 g of biomass at 6 WAIT, regardless of cultivar. Metamifop shows potential as an alternative control option for common bermudagrass present within cool-season turfgrass species.


HortScience ◽  
2005 ◽  
Vol 40 (5) ◽  
pp. 1552-1555 ◽  
Author(s):  
Darren W. Lycan ◽  
Stephen E. Hart

Previous research has demonstrated that bispyribac-sodium can selectively control established annual bluegrass (Poa annua L.) in creeping bentgrass (Agrostis stolonifera L.). Annual bluegrass is also a problematic weed in other cool-season turfgrass species. However, the relative tolerance of other cool-season turfgrass species to bispyribac is not known. Field experiments were conducted at Adelphia, N.J., in 2002 and 2003 to gain understanding of the phytotoxic effects that bispyribac may have on kentucky bluegrass (Poa pratensis L.), perennial ryegrass (Lolium perenne L.), tall fescue (Festuca arundinacea (L.) Schreb.), and chewings fine fescue (Festuca rubra L. subsp. commutata Gaud.). Single applications of bispyribac at 37 to 296 g·ha–1 were applied to mature stands of each species on 11 June, 2002 and 10 June, 2003. Visual injury was evaluated and clippings were collected 35 and 70 days after treatment (DAT). Visual injury at 35 DAT increased as bispyribac rate increased. Kentucky bluegrass was least tolerant to bispyribac with up to 28% injury when applied at 296 g·ha–1. Injury on other species did not exceed 20%. Initial injury on perennial ryegrass, tall fescue, and chewings fine fescue was primarily in the form of chlorosis, while kentucky bluegrass exhibited more severe stunting and thinning symptoms. Bispyribac at rates from 74 to 296 g·ha–1 reduced kentucky bluegrass clipping weights by 19% to 35%, respectively, as compared to the untreated control at 35 DAT in 2002. Initial visual injury on perennial ryegrass, tall fescue, and chewings fine fescue dissipated to ≤5% by 70 DAT. However, recovery of kentucky bluegrass was less complete. These studies suggest that bispyribac-sodium has potential to severely injure kentucky bluegrass. Injury on perennial ryegrass, tall fescue, and chewings fine fescue appears to be less severe and persistent; therefore, bispyribac can be used for weed control in these species. Chemical names used: 2,6-bis[(4,6-dimethoxy-2-pyrimidinyl)oxy]benzoic acid (bispyribac-sodium).


Weed Science ◽  
1982 ◽  
Vol 30 (1) ◽  
pp. 116-124 ◽  
Author(s):  
B. J. Johnson

Four herbicides were applied in the spring and fall over a 3-yr period to Kentucky bluegrass [Poa pratensis (L.) ‘Common′] in the Mountain Region and bermudagrass [Cynodon dactylon (L.) Pers. ‘Common′] in the Piedmont Region of Georgia at different frequencies of treatments for summer and winter weed control. Large crabgrass [Digitaria sanguinalis (L.) Scop.] was controlled throughout the 3-yr period from spring treatments of bensulide [O,O-diisopropyl phosphorodithioate S-ester with N-(2-mercaptoethyl)benzenesulfonamide] at 11.2 kg/ha and oxadiazon [2-tert-butyl-4-(2,4-dichloro-5-isopropoxyphenyl)-δ2-1,3,4-oxadiazolin-5-one] at 4.5 kg/ha in the first year followed by 5.6 kg/ha for bensulide and 2.3 kg/ha for oxadiazon the following 2 yr. To control large crabgrass with benefin (N- butyl-N-ethyl-α,α,α-trifluoro-2,6-dinitro-p-toluidine) it was necessary to apply 3.4 kg/ha for 2 consecutive yr before rates could be reduced to 1.7 kg/ha. Oxadiazon was the only herbicide that controlled goosegrass [Eleusine indica (L.) Gaertn.] completely. Control was excellent throughout the 3-yr period when oxadiazon was applied at full rate in the spring of the first year with no additional treatments during the following 2 yr. Optimum control of winter weeds was obtained throughout the 3-yr period when herbicides were applied at full rates for the spring and fall treatments the first year followed by one-half rates at similar dates the following 2 yr. Bensulide treatments increased cover of corn speedwell (Veronica arvensis L.) and hop clover (Trifolium agrarium L.); DCPA (dimethyl tetrachloroterephthalate) and benefin increased spur weed (Soliva spp.) and wild parsnip (Pastinaca sativa L.); and oxadiazon increased wild parsnip and thymeleaf sandwort (Arenaria serpyllifolia L.).


2006 ◽  
Vol 20 (4) ◽  
pp. 1015-1022 ◽  
Author(s):  
Darren W. Lycan ◽  
Stephen E. Hart

Response of creeping bentgrass, annual bluegrass, and Kentucky bluegrass to foliar, soil, or foliar plus soil applications of bispyribac-sodium was evaluated in greenhouse studies. Soil-alone and foliar plus soil applications of bispyribac-sodium at 148 or 296 g ai/ha resulted in greater injury and shoot dry weight reduction of all species 28 d after treatment (DAT) compared to foliar-alone treatments. Creeping bentgrass was less injured than annual or Kentucky bluegrass regardless of application placement. Further studies evaluated foliar and root absorption and translocation of14C-bispyribac-sodium in creeping bentgrass, annual bluegrass, Kentucky bluegrass, and roughtstalk bluegrass. Foliar absorption into creeping bentgrass was less than that of other species at most harvest timings from 4 to 72 h after treatment. Annual and roughstalk bluegrass translocated greater amounts of foliar-absorbed14C to the crown and shoots compared to creeping bentgrass. Annual and roughstalk bluegrass accumulated approximately 47% more14C per dry weight of plant tissue than creeping bentgrass and 74% more than Kentucky bluegrass after 72 h in nutrient solution containing14C-bispyribac-sodium. Annual and roughstalk bluegrass translocated approximately 80% of root-absorbed14C to shoots, whereas creeping bentgrass and Kentucky bluegrass translocated slightly less (66% of absorbed for both species). These studies suggest that bispyribac-sodium is readily absorbed by roots and translocated to shoots which may contribute to its total activity within a plant. In addition, creeping bentgrass displayed lower amounts of foliar and root absorption and subsequent translocation than annual and roughstalk bluegrass which may contribute to greater bispyribac-sodium tolerance displayed by creeping bentgrass.


2009 ◽  
Vol 23 (4) ◽  
pp. 519-523 ◽  
Author(s):  
Patrick E. McCullough ◽  
Stephen E. Hart

Spray adjuvants may enhance bispyribac–sodium efficacy for annual bluegrass control but chelated iron may be needed to reduce potential turf discoloration. Field and laboratory experiments were conducted to investigate the influence of iron and adjuvants on bispyribac–sodium efficacy for annual bluegrass control in cool-season turf. In laboratory experiments,14C–bispyribac–sodium foliar absorption increased in four grasses by approximately 50 and 100% when applied with a nonionic surfactant and methylated seed oil, respectively, compared to the herbicide alone. Chelated iron did not reduce14C–bispyribac–sodium absorption. In field experiments, spray adjuvants enhanced annual bluegrass control from bispyribac–sodium at 37 g ai/ha but not at 74 g ai/ha. Iron did not reduce annual bluegrass control from bispyribac–sodium, with or without adjuvants, but mitigated creeping bentgrass discoloration for all treatments.


Weed Science ◽  
2016 ◽  
Vol 64 (3) ◽  
pp. 399-408 ◽  
Author(s):  
Patrick E. McCullough ◽  
Jialin Yu ◽  
Paul L. Raymer ◽  
Zhengbeng Chen

A goosegrass biotype with suspected resistance to acetyl-CoA carboxylase (ACCase) inhibitors was identified in Georgia. The objectives of this research were to evaluate the resistance level of this biotype to ACCase inhibitors, efficacy of various herbicide mechanisms of action for control, and the physiological and molecular basis of resistance. In greenhouse experiments, the rate of diclofop-methyl that reduced dry shoot biomass 50% (SR50) from the nontreated for the resistant (R) and susceptible (S) biotypes measured 4,100 and 221 g ai ha−1, respectively. The SR50for sethoxydim measured 615 and 143 g ai ha−1for the R and S biotype, respectively. The R biotype was cross resistant to clethodim, fenoxaprop, and fluazifop. The R and S biotypes were equally susceptible to foramsulfuron, glyphosate, monosodium methylarsenate (MSMA), and topramezone. In laboratory experiments, the two biotypes had similar foliar absorption of14C-diclofop-methyl. Both biotypes metabolized14C-diclofop-methyl to diclofop acid and a polar conjugate, but the R biotype averaged ∼2 times greater metabolism than the S biotype. Gene sequencing revealed an Asp-2078-Gly substitution in the ACCase of the R biotype that has previously conferred resistance to ACCase inhibitors. A second mutation was identified in the R biotype that yielded a Thr-1805-Ser substitution that has been previously reported, but is not associated with ACCase resistance in other species. Thus, the Asp-2078-Gly substitution is the basis for resistance to ACCase inhibitors for the R biotype. This is the first report of ACCase-inhibitor resistance in goosegrass from the United States and from a turfgrass system.


10.1614/183.1 ◽  
2006 ◽  
Vol 20 (2) ◽  
pp. 526-529 ◽  
Author(s):  
Darren W. Lycan ◽  
Stephen E. Hart

Field studies were conducted in 2002 and 2003 in New Jersey to determine the length of time after a bispyribac-sodium application at which creeping bentgrass, Kentucky bluegrass, and perennial ryegrass can be safely reseeded. Bispyribac at 148 or 296 g ai/ha was applied 6, 4, 2, or 1 week before seeding (WBS). Bispyribac at 148 g/ha applied 1 WBS reduced ground cover of creeping bentgrass and Kentucky bluegrass at 3 weeks after seeding (WAS) by 30 and 42% as compared to the nontreated check, respectively. Reductions in Kentucky bluegrass and creeping bentgrass ground cover from bispyribac at 296 g/ha applied 1 WBS were evident at 28 WAS, whereas perennial ryegrass recovered from initial reductions in ground cover by this time. Applications made 6 to 2 weeks before seeding did not adversely affect ground cover of any species at any evaluation date as compared to the nontreated check. These studies suggest creeping bentgrass, Kentucky bluegrass, and perennial ryegrass can be safely reseeded 2 weeks after a bispyribac application. However, ground cover may be reduced by bispyribac applied 1 WBS.


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