ACCase-Inhibiting Herbicide-ResistantAvenaspp. Populations from the Western Australian Grain Belt

Avenaspp. are world weeds with many cases of evolved herbicide resistance. In Australia,Avenaspp. (wild oat and sterile oat) are a major problem, especially in grain crops. Acetyl-CoA carboxylase (ACCase)–inhibiting herbicides have been used extensively since the late 1970s forAvenaspp. control. However, continued reliance on these herbicides has resulted in the evolution of resistantAvenaspp. populations. Resistance across many ACCase-inhibiting herbicides was characterized in fourAvenaspp. populations from the Western Australian grain belt. Dose–response experiments were conducted to determine the level of resistance to the aryloxyphenoxypropionates and cyclohexanediones and to the phenylpyrazoline herbicide pinoxaden. On the basis of resistance index values, all four resistant populations exhibited high-level diclofop resistance but varied in the level of resistance to other ACCase-inhibiting herbicides tested. It is evident thatAvenaspp. populations from the Western Australian grain belt have evolved resistance to a number of ACCase-inhibiting herbicides.

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An isoleucine to leucine mutation in acetyl-CoA carboxylase confers herbicide resistance in wild oat

Genome ◽

10.1139/g02-080 ◽

2002 ◽

Vol 45 (6) ◽

pp. 1049-1056 ◽

Cited By ~ 51

Author(s):

Michael J Christoffers ◽

Michelle L Berg ◽

Calvin G Messersmith

Keyword(s):

Herbicide Resistance ◽

Dna Sequences ◽

Resistance Mutation ◽

Triticum Aestivum L ◽

Wild Oat ◽

Acetyl Coa Carboxylase ◽

Acetyl Coa ◽

Herbicide Resistant ◽

Binding Domains ◽

Substitution Site

Wild oat (Avena fatua L.) populations resistant to herbicides that inhibit acetyl-CoA carboxylase (ACCase; EC 6.4.1.2) represent an increasingly important weed control problem. The objective of this study was to determine the ACCase mutation responsible for herbicide resistance in a well-studied wild oat biotype (UM1). A 2039-bp region encompassing the carboxybiotin and acetyl-CoA binding domains of multifunctional plastidic ACCase was analyzed. DNA sequences representing three plastidic ACCase gene loci were isolated from both the resistant UM1 and a herbicide-susceptible biotype, consistent with the hexaploid nature of wild oat. Only one nonsynonymous point mutation was found among the resistant wild oat sequences, inferring an isoleucine to leucine substitution. The position of this substitution corresponds to residue 1769 of wheat (Triticum aestivum L.) plastidic ACCase (GenBank accession No. AF029895). Analysis of an F2 population derived from a cross between a herbicide-resistant and a susceptible biotype confirmed co-segregation of herbicide resistance with the mutated ACCase. We conclude that the isoleucine to leucine mutation is responsible for herbicide resistance in UM1 wild oat based on a comparison of the substitution site across species and ACCase types. While isoleucine is conserved among plastidic ACCases of herbicide-susceptible grasses, leucine is found in plastidic and cytosolic forms of multifunctional herbicide-resistant ACCase.Key words: acetyl-CoA carboxylase, herbicide resistance, mutation, wild oat.

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Characterization of cross-resistance patterns in acetyl-CoA carboxylase inhibitor resistant wild oat (Avena fatua)

Weed Science ◽

10.1017/s0043174500088925 ◽

1997 ◽

Vol 45 (6) ◽

pp. 750-755 ◽

Cited By ~ 34

Author(s):

Luc Bourgeois ◽

Norm C. Kenkel ◽

Ian N. Morrison

Keyword(s):

Cluster Analysis ◽

Principal Component ◽

Petri Dish ◽

Cross Resistance ◽

Wild Oat ◽

Acetyl Coa Carboxylase ◽

Acetyl Coa ◽

Pairwise Correlation ◽

Resistance Patterns

The purpose of this study was to determine cross-resistance patterns among wild oat lines resistant to acetyl-CoA carboxylase (ACCase) inhibitors and to determine which, if any, cross-resistant type was more common than another. Discriminatory concentrations of two aryloxyphenoxy-propionates (APP) and three cyclohexanediones (CHD) were determined using a petri-dish bioassay. These concentrations were then applied to 82 resistant wild oat lines identified in previous studies. In addition, two resistant standards (UM1 and UM33) and a susceptible standard (UM5) were included in the experiments. Coleoptile lengths expressed as percentages of untreated controls were used to assess the level of resistance to each herbicide. Large variations were observed among wild oat lines and herbicides. However, cluster analysis summarized the relationship between the five herbicides (variables) and the wild oat lines into three main cross-resistance types. Type A included wild oat lines with high resistance to APP herbicides and no or low resistance to CHD herbicides. Types B and C included those with low to moderate resistant and high levels of resistance to all five herbicides, respectively. Type C was the most common cross-resistance type. Relationships among herbicides were determined using pairwise correlation and principal component analysis (PCA). All correlations were high between APP herbicides and between CHD herbicides but not between APP and CHD herbicides. The first two axes of the PCA accounted for 88.4% of the total variance, with the first axis correlated to the CHD herbicides and the second axis correlated to the APP herbicides. In the PCA, wild oat lines were segregated into the three types identified in the cluster analysis. Although CHD and APP herbicides bind at the same region on the ACCase, resistant wild oat lines respond differently to them.

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Wild oat (Avena fatua L.) biotypes resistant to acetolactate synthase and acetyl-CoA carboxylase inhibitors in Poland

Plant Soil and Environment ◽

10.17221/177/2013-pse ◽

2013 ◽

Vol 59 (No. 9) ◽

pp. 432-437 ◽

Cited By ~ 7

Author(s):

K. Adamczewski ◽

R. Kierzek ◽

K. Matysiak

Keyword(s):

Resistance Index ◽

Acetolactate Synthase ◽

Avena Fatua ◽

Multiple Resistance ◽

Wild Oat ◽

Acetyl Coa Carboxylase ◽

Site Mutation ◽

Metabolic Resistance ◽

Mechanism Of Resistance ◽

And Control

The aim of the study was to collect seeds of wild oat from the fields where, in spite of the applied herbicides, the weed is very poorly controlled, and to determine under greenhouse conditions if any resistant biotypes are present. In the years 2008–2011, 34 samples of wild oat were collected from fields where the weed was poorly controlled. The biotypes were analyzed in greenhouse experiments to determine if they are resistant to herbicides. Among five resistant biotypes three of them (R3, R4 and R5) were resistant only to iodosulfuron and mesosulfuron, and biotype R2 – only to propoxycarbazone-sodium. Biotype R1 exhibited multiple resistance to iodosulfuron + mesosulfuron and pinoxaden. The use of sulfometuron proves that the mechanism of resistance of two biotypes of wild oat (R1 and R4) to acetolactate synthase inhibitors is associated with target-site mutation. The curve of biotypes R3 and R5 controlled with iodosulfuron + mesosulfuron shows a relatively low resistance index and control of those biotypes with sulfometuron indicates a metabolic resistance.

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Stimulation of hepatic lipogenesis and acetyl-coenzyme A carboxylase by vasopressin

Biochemical Journal ◽

10.1042/bj1980485 ◽

1981 ◽

Vol 198 (3) ◽

pp. 485-490 ◽

Cited By ~ 21

Author(s):

F Assimacopoulos-Jeannet ◽

R M Denton ◽

B Jeanrenaud

Keyword(s):

Fatty Acid ◽

Dose Response ◽

Fatty Acid Synthesis ◽

Response Curve ◽

Acid Synthesis ◽

Dose Response Curve ◽

Dependent Manner ◽

Hepatic Lipogenesis ◽

Acetyl Coa Carboxylase ◽

Acetyl Coa

The effect of vasopressin on the short-term regulation of fatty acid synthesis was studied in isolated hepatocytes from rats fed ad libitum. Vasopressin stimulates fatty acid synthesis by 30-110%. This increase is comparable with that obtained with insulin. Angiotensin also stimulates fatty acid synthesis, whereas phenylephrine does not. The dose-response curve for vasopressin-stimulated lipogenesis is similar to the dose-response curve for glycogenolysis and release of lactate plus pyruvate. Vasopression also stimulates acetyl-CoA carboxylase activity in a dose-dependent manner. Vasopressin does not relieve glucagon-inhibited lipogenesis, whereas insulin does. The action of vasopressin on hepatic lipogenesis is decreased, but not suppressed, in Ca2+-depleted hepatocytes. The results suggest that vasopressin acts on lipogenesis by increasing availability of lipogenic substrate (lactate + pyruvate) and by activating acetyl-CoA carboxylase.

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Characterization of Wild Oat (Avena fatuaL.) Populations and an Inbred Line with Multiple Herbicide Resistance

Weed Science ◽

10.1017/s0043174500094819 ◽

1996 ◽

Vol 44 (4) ◽

pp. 847-852 ◽

Cited By ~ 15

Author(s):

Anthony J. Kern ◽

Corey T. Colliver ◽

Bruce D. Maxwell ◽

Peter K. Fay ◽

William E. Dyer

Keyword(s):

Inbred Line ◽

Herbicide Resistance ◽

Dose Response ◽

Petri Dish ◽

Wild Oat ◽

Repeated Use ◽

Sufficient Magnitude ◽

Multiple Herbicide Resistance

Repeated use of the preemergence herbicide triallate has selected for wild oat populations that are resistant (R) to field use rates. Field collections and an inbred R line were shown in greenhouse and petri dish dose response experiments to be 6- to 20-fold more tolerant to triallate than susceptible (S) lines. R populations and the inbred line were also resistant (8-fold) to the related thiocarbamate herbicide diallate, as well as to the chemically unrelated postemergence herbicide difenzoquat (60-fold).14C-triallate uptake and translocation patterns were similar between R and S lines for the first 24 h after application. However, translocation of radioactivity was more rapid in S lines than R lines from 24 through 60 h after application.14C-difenzoquat uptake was the same in R and S lines 12 h after application, but was 10 to 20% higher in R lines than S lines by 24 through 96 h after application. Similarly, translocation of radioactivity after14C-difenzoquat application was 7 to 14% greater in R than S lines after 12 h, although translocated radioactivity amounts were not significantly different between R and S lines. The relatively minor differences in triallate and difenzoquat uptake and translocation patterns between R and S lines are most likely not of sufficient magnitude to explain the observed resistance levels.

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Resistant Acetyl-CoA Carboxylase is a Mechanism of Herbicide Resistance in a Biotype of Avena sterilis ssp. ludoviciana

Plant and Cell Physiology ◽

10.1093/oxfordjournals.pcp.a078638 ◽

1994 ◽

Vol 35 (4) ◽

pp. 627-635 ◽

Cited By ~ 34

Author(s):

Chanya Maneechote ◽

Joseph A.M. Holtum ◽

Christopher Preston ◽

Stephen B. Powles

Keyword(s):

Herbicide Resistance ◽

Acetyl Coa Carboxylase ◽

Acetyl Coa ◽

Avena Sterilis

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Derived cleaved amplified polymorphic sequence, a simple method to detect a key point mutation conferring acetyl CoA carboxylase inhibitor herbicide resistance in grass weeds

Weed Research ◽

10.1111/j.1365-3180.2006.00487.x ◽

2006 ◽

Vol 46 (1) ◽

pp. 34-39 ◽

Cited By ~ 40

Author(s):

S S KAUNDUN ◽

J D WINDASS

Keyword(s):

Point Mutation ◽

Herbicide Resistance ◽

Acetyl Coa Carboxylase ◽

Acetyl Coa ◽

Simple Method ◽

Cleaved Amplified Polymorphic Sequence

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Fenoxaprop-p-ethyl Susceptibility and Mutation Point Detection of Acetyl-CoA Carboxylase (ACCase) in Different Wild oat (Avena fatua L.) Populations from China

10.1101/549402 ◽

2019 ◽

Author(s):

Jun-jie Liu ◽

Liuyang Lu ◽

Bai-zhong Zhang ◽

Xi-ling Chen

Keyword(s):

Gene Expression ◽

Critical Role ◽

Enzyme Linked Immunosorbent Assay ◽

Wild Oat ◽

Acetyl Coa Carboxylase ◽

Acetyl Coa ◽

Mutation Site ◽

Multiple Sequence ◽

Susceptible Population ◽

In The Wild

AbstractTo explore resistant mechanism of wild oat to fenoxaprop-p-ethyl, the susceptibility of Acetyl-CoA Carboxylase (ACCase) from 24 wild oat populations to fenoxaprop-p-ethyl, the level of gene expression, and mutation site of ACCase were conducted. In vitro ACCase activities were solated and measured by enzyme-linked immunosorbent assay kit (ELISA) assays, the results indicated that the IC50 value of the ACCase of the most unsusceptible to fenoxaprop-p-ethyl in the wild oat population from Yexian2017 (W24) was 7206.557-fold compared to that of the ACCase of most susceptible to fenoxaprop-p-ethyl in the wild oat population from Queshan (W11). The differential expression of genes in wild oat treated by the IC50 fenoxaprop-p-ethyl concentration (6.9 mg/L) for 24 hours using RNA-seq, digital gene expression (DGE) profling was conducted. We found that 8 unigenes annotated as ACCase genes, 0 up-regulaed expression and 3 down-regulated expression were observed. The down-regulaed expressed ACCase was selected for qPCR in the relative susceptible population were significantly more suppressed than the three relative resistant populations. The mutations point of ACCase, Ile-1781-Leu, Trp-1999-Cys, Trp-2027-Cys, Ile-2041-Asn, Asp-2078-Gly, Cys-2088-Arg published were not found in the populations tested by multiple sequence alignment with a model complete ACCase sequence of Alopecurus myosuroides. These findings suggest that ACCase plays a critical role in the development of wild oat resistance to fenoxaprop-p-ethyl.

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Selection and evolution of acetyl-CoA carboxylase (ACC)-inhibitor resistance in wild oat (Avena fatua L.) in a long-term alternative cropping systems study

Canadian Journal of Plant Science ◽

10.4141/cjps2013-361 ◽

2014 ◽

Vol 94 (4) ◽

pp. 727-731 ◽

Cited By ~ 6

Author(s):

Hugh J. Beckie ◽

Eric N. Johnson ◽

Julia Y. Leeson ◽

Scott W. Shirriff ◽

Arlen Kapiniak

Keyword(s):

Cropping Systems ◽

Cropping System ◽

Avena Fatua ◽

Wild Oat ◽

Acetyl Coa Carboxylase ◽

Acetyl Coa ◽

Evolution Of Resistance ◽

Inhibitor Resistance ◽

Herbicide Use

Beckie, H. J., Johnson, E. N., Leeson, J. Y., Shirriff, S. W. and Kapiniak, A. 2014. Selection and evolution of acetyl-CoA carboxylase (ACC)-inhibitor resistance in wild oat (Avena fatua L.) in a long-term alternative cropping systems study. Can. J. Plant Sci. 94: 727–731. In 2012, 18 yr after experiment establishment, wild oat from the spring wheat phase of seven of nine alternative cropping systems (each of three input levels applied to three levels of cropping diversity) were sampled and screened for ACC-inhibitor resistance. The frequency or level of resistance in wild oat was greatest in the diversified annual grains systems (42–60% of individuals), and lowest in the diversified annual perennial systems (<3%). The results of this study demonstrate the importance of perennial crops in slowing the selection and evolution of resistance in this weed. Moreover, annual cropping system diversity by itself is not enough to slow the evolution of ACC-inhibitor resistance in wild oat; cropping diversity must be linked with herbicide mode-of-action diversity and herbicide-use reduction.

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