DIFFERENTIAL IDENTIFICATION OF ASCOGREGARINA SPECIES (APICOMPLEXA: LECUDINIDAE) IN AEDES AEGYPTI AND AEDES ALBOPICTUS (DIPTERA: CULICIDAE) BY POLYMERASE CHAIN REACTION

2005 ◽  
Vol 91 (6) ◽  
pp. 1352-1356 ◽  
Author(s):  
Maria E. Morales ◽  
Clara B. Ocampo ◽  
Horacio Cadena ◽  
Claudia S. Copeland ◽  
Michael Termini ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Differential Identification

scholarly journals Detection of Dengue Virus In Aedes sp. Mosquito at Home of DHF Patients in Ternate City

2017 ◽  
Vol 7 (2) ◽  
pp. 158-166
Author(s):  
Mustafa Mustafa
Keyword(s):  
Polymerase Chain Reaction ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Aedes Albopictus ◽  
Chain Reaction ◽  
Pcr Polymerase Chain Reaction ◽  
Polymerase Chain ◽  
At Home

Virus Dengue adalah penyebab penyakit demam berdarah dengue (DBD), ditularkan melalui gigitan nyamuk Aedes aegypti dan Aedes albopictus. Penyakit Demam Berdarah Dengue (DBD) adalah penyakit infeksi yang disebabkan oleh virus Dengue dengan serotipe DEN-1, DEN-2, DEN-3 dan DEN-4. Penelitian ini bertujuan untuk mengetahui keberadaan virus Dengue pada nyamuk Aedes sp di rumah penderita DBD Kota Ternate. Jenis penelitian yang digunakan adalah penelitian Deskriptif dengan uji laboratorium. Sampel pada penelitian ini adalah nyamuk jenis Aedes sp yang ditangkap dirumah penderita DBD. Teknik pengambilan sampel pada penelitian ini adalah Total sampling. Hasil penelitian ini menunjukkan kondisi lingkungan rumah penderita rata-rata berpotensial untuk perkembangan nyamuk dan jentik, suhu dalam rumah (91,67%), suhu luar rumah (83,33%), suhu air (91,67%), kelembaban (83,33%), pH air (75%) dan pencahayaan (91,67%). Jenis wadah berpotensial untuk perkebangan jentik yaitu bak mandi/WC 57,1%, drum plastik 40 % dan ember 36,8 %. Dari hasil pemeriksaan PCR (Polymerase Chain Reaction)  pada 369 ekor nyamuk Aedes sp tidak menunjukkan adanya nyamuk yang mengandung virus Dengue-1. Kesimpulan dari penelitian ini adalah rata-rata Kondisi lingkungan fisik potensial untuk perkembangan larva dan nyamuk. Jenis wadah bak paling tinggi positif jentik, dan dari semua sampel nyamuk yang diperiksa tidak satupun yang mengandung virus Dengue serotipe DEN1.

Deteksi Transmisi Transovarial Virus Dengue PadaAedes AegyptiPada Wilayah Endemis Kota Pontianak

2021 ◽  
Vol 1 ◽  
pp. 984-995
Author(s):  
Malik Saepudin ◽  
Heru Subaris Kasjono ◽  
M Martini
Keyword(s):  
Polymerase Chain Reaction ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Aedes Albopictus ◽  
Transovarial Transmission ◽  
Chain Reaction ◽  
Cross Sectional ◽  
Transmission Index ◽  
Virus Serotype ◽  
Polymerase Chain

AbstractDengue virus is highly pathogenic in humans and spreads rapidly through Aedes aegypti and Aedes albopictus mosquitoes. More than half a billion people from 100 countries in the world are at serious risk of dengue virus infection. The purpose of this study was to prove the existence of transovarial transmission of dengue virus in Aedes aegypti mosquitoes with a transovarial transmission index (TTI) in endemic areas in Pontianak, West Kalimantan. This research method is descriptive observational with a cross-sectional study. The results of the Microscopic Examination of Head Squash Preparations on the Aedes aegypti mosquito, showed the Transovarial Transmission Index in Batulayang Village was 39.60% higher, compared to Sungai Jawi Village, which was 29.30%, but both were still lower than ITT in 2012. The results of the Aedes mosquito examination aegypti using the Polymerase Chain Reaction Transcription Reaction (PCR-TR) method found the dengue virus strain. The conclusion of this study proves that the transovarial transmission of dengue virus in Aedes aegypti mosquitoes in Sungai Jawi Village is 29.30% lower than in Batulayang Village by 39.60%, and the dengue virus serotype, DENV-3, has been found.Keywords: transovarial transmission; dengue virus; Aedes aegypti AbstrakVirus Dengue sangat patogen pada manusia dan menyebar dengan cepat melalui nyamuk Aedes aegypti dan Aedes albopictus. Lebih dari setengah miliar penduduk dari 100 negara di dunia berada pada risiko serius infeksi virus dengue. Tujuan dari penelitian ini adalah untuk membuktikan adanya penularan virus dengue transovarial pada nyamuk Aedes aegypti dengan indeks transmisi transovarial (TTI) di daerah Endemis di Pontianak, Kalimantan Barat. Metode penelitian ini adalah deskriptif observasional dengan studi potong lintang. Hasil Pemeriksaan Mikroskopis Sediaan Head Squash pada nyamuk Aedes aegypti, menunjukan Indeks Transmisi Transovarial pada Kelurahan Batulayang lebih tinggi yaitu 39,60%, dibandingkan dengan Kelurahan Sungai Jawi yaitu 29,30%, tetapi keduanya masih lebih rendah dibandingkan ITT pada Tahun 2012. Hasil pemeriksaan nyamuk Aedes aegypti dengan metode Polymerase Chain Reaction Transcription Reaction(PCR-TR) ditemukan strain virus Dengue-3. Kesimpulan penelitian ini membuktikan adanya transmisi transovarial virus dengue pada nyamuk Aedes aegypti di Kelurahan Sungai Jawi sebesar 29,30% lebih rendah dibandingkan di kelurahan Batulayang sebesar 39,60%, serta berhasil ditemukan serotipe virus Dengue yaitu DENV-3.Kata Kunci : transmisi transovarial; virus dengue; Aedes aegypti

scholarly journals Use of immunodot blot and multiplex reverse transcriptase-polymerase chain reaction in dengue virus detection in macerates of Aedes aegypti larvae

2012 ◽  
Vol 3 (1) ◽  
pp. 13
Author(s):  
Aline T.A. Chagas ◽  
Michelle D. Oliveira ◽  
Jose M.S. Mezencio ◽  
Eduardo A.M. Silva ◽  
Leandro L. Oliveira ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Reverse Transcriptase ◽  
Epidemiological Surveillance ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Denv Serotypes ◽  
Polymerase Chain ◽  
Pcr Techniques

The <em>Dengue virus</em> is the main arbovirus that affects man in terms of morbidity and mortality. The detection of the virus is very important for epidemiological surveillance, so here we propose to standardize and compare the immunodot blot (IDB) and multiplex reverse transcriptase-polymerase chain reaction (M-RT-PCR) techniques to detect and characterize the dengue virus (DENV) serotypes in samples of <em>Aedes aegypti</em> larvae. Thus, the IDB and M-RT-PCR techniques were standardized using macerated samples of larvae collected in nature. The use of monoclonal antibodies in IDB has not shown great results, but DENV detection through this method was possible using polyclonal antibodies. The distinction of serotypes 1, 2 and 3 was carried out by M-RT-PCR.

scholarly journals Report of Chikungunya Virus in Wild Populations of Aedes aegypti in Guerrero State, Mexico

2018 ◽  
Vol 34 (2) ◽  
pp. 147-150 ◽  
Author(s):  
Gustavo Ponce-García ◽  
Adriana E. Flores-Suarez ◽  
Karina Villanueva-Segura ◽  
Martha Lopez-Rodriguez ◽  
Felipe Dzul ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Aedes Aegypti ◽  
Reverse Transcriptase ◽  
Real Time ◽  
Chikungunya Virus ◽  
Wild Populations ◽  
Chain Reaction ◽  
Male And Female ◽  
San Marcos ◽  
Polymerase Chain

ABSTRACT We detected vertical transmission of chikungunya virus (CHIKV) in wild populations of Aedes aegypti from San Marcos, Guerrero, Mexico, with real-time reverse transcriptase–polymerase chain reaction. A total of 20 pools (1–11 specimens/pool) of larvae, male, and female mosquitoes were tested. We report the detection of CHIKV in 2 of 11 larval pools, 4 of 5 male pools, and 1 of 4 female pools, from field-collected mosquitoes.

scholarly journals MUTASI TITIK F1534C GEN VOLTAGE GATED SODIUM CHANNEL (VGSC) PADA Aedes aegypti DI KELURAHAN PANCUR PUNGAH

2020 ◽  
Vol 8 (1) ◽  
pp. 26-33
Author(s):  
Fadjar Sidiq Hidayahtullah ◽  
Chairil Anwar ◽  
Dwi Handayani
Keyword(s):  
Polymerase Chain Reaction ◽  
Aedes Aegypti ◽  
Hemorrhagic Fever ◽  
Chain Reaction ◽  
Mutation Site ◽  
Mosquito Mortality ◽  
The World ◽  
Polymerase Chain ◽  
Pcr Test ◽  
F1 Generation

The incidence of dengue hemorrhagic fever (DHF) has increased significantly throughout the world in the last decade. One of the challenges in eradicating DHF is the occurrence of vector resistance to insecticides. This study aimed to looked at the mutation site of the VGSC codon F1534C Aedes aegypti on RT 5 & 6 Pancur Pungah Village, Muara Dua District, Ogan Komering Ulu Selatan Regency as a marker of resistance. The samples in this studied were larvae and pupae of Aedes aegypti. Larvae and pupae were bred into F1 generation and then tested for susceptibility and followed by polymerase chain reaction (PCR). The results of the suscepbillity test carried out obtained mosquito mortality of 10.5% so it was categorized resistant based on WHO criteria. Continued PCR test found no mutation point occurred in the VGSC gene mutation point F1534C in Aedes aegypti. Therefore, the possibility of mutations in the VGSC gene is at another mutation point. Keyword: Mutation, Aedes aegypti, F1534C   ABSTRAK Kejadian demam berdarah dengue (DBD) telah meningkat signifikan di seluruh dunia dalam dekade terakhir. Salah satu tantangan dalam pemberantasan DBD adalah kejadian resistensi vektor terhadap insektisida. Penelitian ini bertujuan untuk melihat titi mutasi gen VGSC kodon F1534C Aedes aegypti di RT 5 & 6 Kelurahan Pancur Pungah Kecamatan Muara Dua Kabupaten Ogan Komering Ulu Selatan sebagai penanda terjadinya resistensi. Sampel pada penelitian ini adalah larva serta pupa nyamuk Aedes aegypti. Larva dan pupa dibiakkan menjadi generasi F1 lalu dilakukan uji suscepbillity dan dilanjutkan uji polymerase chain reaction (PCR).  Hasil penelitian pada uji  suscepbillity yang dilakukan didapat kematian nyamuk sebesar 10,5% sehingga dikategorikan resisten berdasarkan kriteria WHO. Dilanjutkan uji PCR didapat tidak terjadi titik mutasi pada gen VGSC mutasi titik F1534C pada Aedes aegypti. Oleh karena itu, kemungkinan terjadinya mutasi pada gen VGSC berada pada titik mutasi yang lain. Kata kunci: Mutasi, Aedes aegypti, F1534C

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