Monitoring of dengue viruses in field-caught Aedes aegypti and Aedes albopictus mosquitoes by a type-specific polymerase chain reaction and cycle sequencing.

1998 ◽  
Vol 58 (5) ◽  
pp. 578-586 ◽  
Author(s):  
V T Chow ◽  
Y K Chung ◽  
S G Lam-Phua ◽  
K M Lee ◽  
R Yong ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Chain Reaction ◽  
Cycle Sequencing ◽  
Dengue Viruses ◽  
Specific Polymerase Chain Reaction ◽  
Polymerase Chain

scholarly journals Detection of Dengue Virus In Aedes sp. Mosquito at Home of DHF Patients in Ternate City

2017 ◽  
Vol 7 (2) ◽  
pp. 158-166
Author(s):  
Mustafa Mustafa
Keyword(s):  
Polymerase Chain Reaction ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Aedes Albopictus ◽  
Chain Reaction ◽  
Pcr Polymerase Chain Reaction ◽  
Polymerase Chain ◽  
At Home

Virus Dengue adalah penyebab penyakit demam berdarah dengue (DBD), ditularkan melalui gigitan nyamuk Aedes aegypti dan Aedes albopictus. Penyakit Demam Berdarah Dengue (DBD) adalah penyakit infeksi yang disebabkan oleh virus Dengue dengan serotipe DEN-1, DEN-2, DEN-3 dan DEN-4. Penelitian ini bertujuan untuk mengetahui keberadaan virus Dengue pada nyamuk Aedes sp di rumah penderita DBD Kota Ternate. Jenis penelitian yang digunakan adalah penelitian Deskriptif dengan uji laboratorium. Sampel pada penelitian ini adalah nyamuk jenis Aedes sp yang ditangkap dirumah penderita DBD. Teknik pengambilan sampel pada penelitian ini adalah Total sampling. Hasil penelitian ini menunjukkan kondisi lingkungan rumah penderita rata-rata berpotensial untuk perkembangan nyamuk dan jentik, suhu dalam rumah (91,67%), suhu luar rumah (83,33%), suhu air (91,67%), kelembaban (83,33%), pH air (75%) dan pencahayaan (91,67%). Jenis wadah berpotensial untuk perkebangan jentik yaitu bak mandi/WC 57,1%, drum plastik 40 % dan ember 36,8 %. Dari hasil pemeriksaan PCR (Polymerase Chain Reaction)  pada 369 ekor nyamuk Aedes sp tidak menunjukkan adanya nyamuk yang mengandung virus Dengue-1. Kesimpulan dari penelitian ini adalah rata-rata Kondisi lingkungan fisik potensial untuk perkembangan larva dan nyamuk. Jenis wadah bak paling tinggi positif jentik, dan dari semua sampel nyamuk yang diperiksa tidak satupun yang mengandung virus Dengue serotipe DEN1.

Deteksi Transmisi Transovarial Virus Dengue PadaAedes AegyptiPada Wilayah Endemis Kota Pontianak

2021 ◽  
Vol 1 ◽  
pp. 984-995
Author(s):  
Malik Saepudin ◽  
Heru Subaris Kasjono ◽  
M Martini
Keyword(s):  
Polymerase Chain Reaction ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Aedes Albopictus ◽  
Transovarial Transmission ◽  
Chain Reaction ◽  
Cross Sectional ◽  
Transmission Index ◽  
Virus Serotype ◽  
Polymerase Chain

AbstractDengue virus is highly pathogenic in humans and spreads rapidly through Aedes aegypti and Aedes albopictus mosquitoes. More than half a billion people from 100 countries in the world are at serious risk of dengue virus infection. The purpose of this study was to prove the existence of transovarial transmission of dengue virus in Aedes aegypti mosquitoes with a transovarial transmission index (TTI) in endemic areas in Pontianak, West Kalimantan. This research method is descriptive observational with a cross-sectional study. The results of the Microscopic Examination of Head Squash Preparations on the Aedes aegypti mosquito, showed the Transovarial Transmission Index in Batulayang Village was 39.60% higher, compared to Sungai Jawi Village, which was 29.30%, but both were still lower than ITT in 2012. The results of the Aedes mosquito examination aegypti using the Polymerase Chain Reaction Transcription Reaction (PCR-TR) method found the dengue virus strain. The conclusion of this study proves that the transovarial transmission of dengue virus in Aedes aegypti mosquitoes in Sungai Jawi Village is 29.30% lower than in Batulayang Village by 39.60%, and the dengue virus serotype, DENV-3, has been found.Keywords: transovarial transmission; dengue virus; Aedes aegypti AbstrakVirus Dengue sangat patogen pada manusia dan menyebar dengan cepat melalui nyamuk Aedes aegypti dan Aedes albopictus. Lebih dari setengah miliar penduduk dari 100 negara di dunia berada pada risiko serius infeksi virus dengue. Tujuan dari penelitian ini adalah untuk membuktikan adanya penularan virus dengue transovarial pada nyamuk Aedes aegypti dengan indeks transmisi transovarial (TTI) di daerah Endemis di Pontianak, Kalimantan Barat. Metode penelitian ini adalah deskriptif observasional dengan studi potong lintang. Hasil Pemeriksaan Mikroskopis Sediaan Head Squash pada nyamuk Aedes aegypti, menunjukan Indeks Transmisi Transovarial pada Kelurahan Batulayang lebih tinggi yaitu 39,60%, dibandingkan dengan Kelurahan Sungai Jawi yaitu 29,30%, tetapi keduanya masih lebih rendah dibandingkan ITT pada Tahun 2012. Hasil pemeriksaan nyamuk Aedes aegypti dengan metode Polymerase Chain Reaction Transcription Reaction(PCR-TR) ditemukan strain virus Dengue-3. Kesimpulan penelitian ini membuktikan adanya transmisi transovarial virus dengue pada nyamuk Aedes aegypti di Kelurahan Sungai Jawi sebesar 29,30% lebih rendah dibandingkan di kelurahan Batulayang sebesar 39,60%, serta berhasil ditemukan serotipe virus Dengue yaitu DENV-3.Kata Kunci : transmisi transovarial; virus dengue; Aedes aegypti

DIFFERENTIAL IDENTIFICATION OF ASCOGREGARINA SPECIES (APICOMPLEXA: LECUDINIDAE) IN AEDES AEGYPTI AND AEDES ALBOPICTUS (DIPTERA: CULICIDAE) BY POLYMERASE CHAIN REACTION

2005 ◽  
Vol 91 (6) ◽  
pp. 1352-1356 ◽  
Author(s):  
Maria E. Morales ◽  
Clara B. Ocampo ◽  
Horacio Cadena ◽  
Claudia S. Copeland ◽  
Michael Termini ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Differential Identification

Routine identification of four sympatric species of calanoid copepods Pseudocalanus spp. in the Atlantic Arctic using a species-specific polymerase chain reaction

2020 ◽  
Vol 48 (1) ◽  
pp. 62-72
Author(s):  
E. A. Ershova
Keyword(s):  
Polymerase Chain Reaction ◽  
Life Cycles ◽  
The Arctic ◽  
Relative Distribution ◽  
Chain Reaction ◽  
Specific Polymerase Chain Reaction ◽  
Routine Identification ◽  
Polymerase Chain ◽  
Species Specific ◽  
Plankton Communities

Сalanoid copepods of the genus Pseudocalanus play an important role in the plankton communities of the Arctic and boreal seas, often dominating in numbers and constituting a significant proportion of the biomass of zooplankton. Despite their high presence and significance in the shelf plankton communities, species-specific studies of the biology of these are significantly hampered by extremely small morphological differences between them, especially at the juvenile stages, at which they are virtually indistinguishable. In this paper, we describe a new, routine and low-cost molecular method for identifying all Pseudocalanus species found in the Atlantic sector of the Arctic: the Arctic P. acuspes, P. minutus and the boreal P. moultoni and P. elongatus, and apply it to describe the relative distribution of these species in four locations of the Arctic and sub-Arctic. With this method, species-specific polymerase chain reaction (ssPCR), mass identification of individuals of any developmental stage, including nauplii, is possible. This method can serve as an excellent tool for studying the species-specific biology of this group, describing their life cycles, as well as monitoring changes in Arctic marine ecosystems under the influence of changing climate.

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