SEQUENTIAL FOETAL—MATERNAL PLASMA CORTISOL LEVELS IN SHEEP

1973 ◽  
Vol 56 (3) ◽  
pp. 483-492 ◽  
Author(s):  
M. DROST ◽  
L. F. KUMAGAI ◽  
MARGARET GUZMAN
Keyword(s):  
Protein Binding ◽  
Plasma Cortisol ◽  
Maternal Plasma ◽  
Blood Samples ◽  
Serial Blood ◽  
Competitive Protein Binding ◽  
Cortisol Levels ◽  
Amino Glutethimide

SUMMARY Chronic foetal—maternal cortisol interrelationships were studied in five time-dated pregnant ewes. Serial blood samples were obtained from ewe and foetus simultaneously for several weeks before and including parturition. Plasma cortisol was measured by both fluorometry and isotopic competitive protein binding. The data indicate that maternal cortisol levels are slightly higher than those in the foetus during the latter part of gestation until approximately 1 week before parturition. During the last week of gestation, especially 4–5 days before delivery, a marked increase in foetal cortisol levels was noted. Administration of amino-glutethimide to the foetus 1 week before delivery did not alter cortisol levels in either foetus or ewe. The results suggest that parturition in sheep is a culmination of events initiated by active foetal adrenocortical secretion.

Morning Plasma Cortisol Levels in Infants Treated with Topical Fluorinated Glucocorticosteroids

PEDIATRICS ◽  
1980 ◽  
Vol 66 (1) ◽  
pp. 102-102
Author(s):  
William L. Weston ◽  
W. Mitchell Sams ◽  
Helen G. Morris ◽  
James M. Arthur ◽  
Gordon J. Blakeman ◽  
...  
Keyword(s):  
Protein Binding ◽  
Coefficient Of Variation ◽  
Plasma Cortisol ◽  
Oral Prednisone ◽  
Body Fluids ◽  
Fluorometric Method ◽  
Competitive Protein Binding ◽  
Cortisol Levels

In the article "Morning Plasma Cortisol Levels in Infants Treated with Topical Fluorinated Glucocorticosteroids" by Weston et al (Pediatrics 65:103, 1980) an error has been noted in the description of the method used for cortisol assay. Samples were assayed by the competitive-protein binding radioassay of Murphy, which permits measurement of cortisol in 0.1-ml aliquots of plasma (0.2 ml for a duplicate determination) with a coefficient of variation of 7%.1,2 The Spencer-Peet fluorometric method, noted incorrectly in the text, would require a much larger volume of plasma than that obtained from the infants. References: (1) Murphy BEP: Some studies of the protein-binding of steroids and their application to the routine micro and ultramicro measurement of various steroids in body fluids by competitive protein binding radioassay. J Clin Endocrinol Metab 27:973, 1967; (2) Morris HG, DeRoche G, Winkler SM, et al: Effect of oral prednisone on the measurement of plasma steroid concentrations by the competitive protein binding radioassay. J Pediatr 85:248, 1974.

scholarly journals Assessment of a Simple Competitive Protein-Binding Technique for Plasma Cortisol Assay

1975 ◽  
Vol 12 (1-6) ◽  
pp. 66-69 ◽  
Author(s):  
Mary F. Crowley ◽  
Katherine J. T. Garbien ◽  
J. W. Tuttlebee
Keyword(s):  
Protein Binding ◽  
Plasma Cortisol ◽  
Late Pregnancy ◽  
Plasma Samples ◽  
Blood Samples ◽  
Prednisolone Therapy ◽  
Dexamethasone Therapy ◽  
Competitive Protein Binding ◽  
Synthetic Steroids ◽  
Significant Interference

The Cortipac kit for Cortisol assay by a competitive protein-binding technique (CPB) which utilizes 75Se Cortisol has been evaluated. Results obtained by it agree well with those by the Mattingly fluorimetric method. Assays can be carried out on either plasma or serum and haemolysis does not interfere. The specificity of the assay was checked in blood samples from patients receiving synthetic steroids. Prednisone and prednisolone therapy caused significant interference with the assay; fluorocortisol and dexamethasone therapy did not. The increased progesterone in late pregnancy blood samples had only a small effect on the assay. Plasma samples for Cortisol assay could be stored for at least 4 weeks at 4°C and for at least 12 weeks at −20°C.

MEASUREMENT OF PLASMA CORTISOL LEVELS IN THE EEL ANGUILLA ANGUILLA IN RELATION TO OSMOTIC ADJUSTMENTS

1971 ◽  
Vol 50 (1) ◽  
pp. 75-96 ◽  
Author(s):  
J. N. BALL ◽  
I. CHESTER JONES ◽  
M. E. FORSTER ◽  
G. HARGREAVES ◽  
E. F. HAWKINS ◽  
...  
Keyword(s):  
Plasma Cortisol ◽  
Sea Water ◽  
Anguilla Anguilla ◽  
Plasma Cortisol Level ◽  
Hormonal Control ◽  
Total Cortisol ◽  
Competitive Protein Binding ◽  
Low Levels ◽  
Cortisol Levels

SUMMARY The competitive protein-binding radioassay (CPB method) of Murphy (1967) has been adapted to determine total cortisol levels in the plasma of the eel, Anguilla anguilla L. Validation of the method for this species depended in part on the development of a chromatographic—fluorimetric technique for eel cortisol, following classical procedures and using radioactive tracers; by this means, the specificity of the CPB method for cortisol in eel plasma was established. Accuracy, precision and sensitivity of the CPB method were also investigated and were shown to be satisfactory. Plasma total cortisol levels were determined in eels during osmotic adjustments after transfers from fresh water (FW) to sea-water (SW) and vice versa, and from FW to distilled water. Plasma osmotic pressure and/or sodium levels were monitored simultaneously, to follow the progress of osmotic regulation. In only one of the transfer situations did the plasma cortisol level change significantly, showing a marked transitory increase during the first few days after transfer from FW to SW, corresponding to the development and correction of an 'osmotic crisis'. Plasma cortisol levels were the same in eels adapted for long periods to FW and to SW. Plasma cortisol fell to extremely low levels after hypophysectomy. These results are discussed in the light of the literature on hormonal control of osmoregulatory mechanisms in the eel, with particular emphasis on the role of adrenocorticosteroids in ionic regulation of animals in SW.

Improved protein-binding radioassay for plasma cortisol.

1980 ◽  
Vol 26 (2) ◽  
pp. 312-313
Author(s):  
H Novacenko ◽  
F Halpern ◽  
E J Sachar
Keyword(s):  
Protein Binding ◽  
Excellent Agreement ◽  
Filter Paper ◽  
Plasma Cortisol ◽  
Rapid Analysis ◽  
Paper Strip ◽  
Total Plasma ◽  
Ethanol Extraction ◽  
Filter Paper Strip ◽  
Competitive Protein Binding

Abstract We describe an improved competitive protein-binding radioassay for total plasma cortisol, which permits rapid analysis of many samples. Plasma, 0.1 mL, is directly applied to a Whatman 3MM filter-paper strip, which is extracted once with dichloromethane. The extract is evaporated and the residue is assayed directly by the micro-method of Murphy (J. Clin. Endocrinol. Metab. 27: 973, 1967). Comparison with the original Murphy method (ethanol extraction) yielded excellent agreement (r = 0.988). One hundred samples, in duplicate, can easily be assayed at a time.

Reliability of Plasma Cortisol Determination by a Simple Competitive Protein Binding Method

1976 ◽  
Vol 13 (1-6) ◽  
pp. 371-378 ◽  
Author(s):  
G. C. Cashmore ◽  
J. D. Few ◽  
Christine H. Fowles
Keyword(s):  
Protein Binding ◽  
Human Plasma ◽  
Cortisol Level ◽  
Plasma Cortisol ◽  
Binding Protein ◽  
Venous Occlusion ◽  
Plasma Cortisol Level ◽  
Short Term ◽  
Term Variation ◽  
Competitive Protein Binding

The method investigated used [3H] cortisol as the ligand, diluted human plasma as the binding protein, and florisil to separate free and bound cortisol. The precision and specificity were found to be adequate for routine use. The effects of such factors as delay in separation of plasma, storage of plasma, provenance of the binding protein, venepuncture, and venous occlusion have been studied. The extent and significance of short term, inter-daily, and longer term variation in plasma cortisol level are discussed.

scholarly journals Selected Factors that Affect the Measurement of Plasma Progesterone Concentrations in Pregnant Ewes

1974 ◽  
Vol 27 (6) ◽  
pp. 659 ◽  
Author(s):  
AR Gleeson ◽  
GD Thorburn
Keyword(s):  
Protein Binding ◽  
Diurnal Variation ◽  
Significant Positive Correlation ◽  
Plasma Samples ◽  
Elevated Plasma ◽  
Progesterone Concentration ◽  
Plasma Progesterone ◽  
Blood Samples ◽  
Peripheral Plasma ◽  
Competitive Protein Binding

A competitive protein-binding technique was used to measure progesterone concentrations in the peripheral plasma of pregnant ewes. Neither haemolysis of blood nor thawing of plasma samples affected plasma progesterone concentration. Blood samples should be chilled immediately upon collection but subsequent to centrifugation immediate chilling of the plasma samples is not critical. No consistent diurnal variation in progesterone concentrations was evident but there was large apparently random day-to-day variation in progesterone concentrations for any ewe. Although a significant positive correlation was found between endogenous progesterone and corticosteroid concentrations, the present study failed to correlate experimentally elevated plasma corticosteroid concentrations with progesterone concentrations. Progesterone concentrations varied greatly between ewes at the same stage of pregnancy.

scholarly journals Pilot Study of the Relationship between Deck Level and Journey Duration on Plasma Cortisol, Epinephrine and Norepinephrine Levels in Italian Heavy Pigs

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1578
Author(s):  
Giancarlo Bozzo ◽  
Barbara Padalino ◽  
Elisabetta Bonerba ◽  
Roberta Barrasso ◽  
Vincenzo Tufarelli ◽  
...  
Keyword(s):  
Pilot Study ◽  
Plasma Cortisol ◽  
Structural Characteristics ◽  
Stress Hormones ◽  
Plasma Concentrations ◽  
Activation Patterns ◽  
Blood Samples ◽  
Cortisol Levels ◽  
Council Regulation ◽  
The Relationship

The aim of this pilot study was to evaluate the relationship between journey duration, deck level and activation patterns of the hypothalamic-pituitary-adrenocortical axis (HPA) and sympathetic adrenal medullary system (SAM) in pigs. A total of 90 pigs were examined. The animals came from three different Italian farms associated with the same slaughterhouse located in Bari (Apulia region-Italy). A group of thirty animals was transported from Pordenone (11 h journey); a second group was transported from Terni (6.5 h journey); a third group was transported from Benevento (3 h journey). The animals were transported in the same vehicle, which complied with the structural characteristics indicated in the Council Regulation (EC) No. 1/2005. The truck was composed of a lorry and a trailer, each one divided into three decks. Only the animals transported in the trailer were tested for the study. Before transportation, blood samples were collected on each farm, at 6:00 a.m., from 30 pigs randomly selected out of 135 pigs ready to be transported. Blood samples were also collected during slaughter to evaluate plasma cortisol, epinephrine and norepinephrine, around 6:00 a.m. A journey duration of 11 h was associated with significantly higher plasma concentrations of stress hormones compared with shorter journeys. This increase was proportional to the journey duration, with the pigs travelling for 6.5 h displaying intermediate concentrations between those noticed after 3 h and 11 h journeys. The interaction between deck and journey distance was not significant on epinephrine, norepinephrine or cortisol levels collected at arrival. There was a significant effect of deck level on norepinephrine levels (p < 0.0001), a tendency to influence epinephrine levels (p = 0.073) but no effect on cortisol levels (p = 0.945). Overall, we observed that an 11 h-long journey seemed to impact negatively on pigs’ HPA-SAM activity, likely requiring the animals to spend more time in the lairage facilities to recover.

Nonspecific Interferences in the Fluorometric Determination of Plasma Cortisol

1973 ◽  
Vol 19 (2) ◽  
pp. 258-261 ◽  
Author(s):  
Thomas R Koch ◽  
Leila Edwards ◽  
Max E Chilcote
Keyword(s):  
Protein Binding ◽  
Plasma Cortisol ◽  
Plasma Sample ◽  
Fluorometric Assay ◽  
Fluorometric Determination ◽  
Clin Pathol ◽  
Optical Arrangement ◽  
Competitive Protein Binding ◽  
The Individual

Abstract The popular Mattingly [J. Clin. Pathol. 15, 374 (1962)] fluorometric assay for plasma cortisol is shown to give rise to falsely high values when performed with the recommended optical arrangement. By exciting at 470 nm, however, results are obtained that correlate well (r = 0.972) with those of competitive protein binding. The source of the interference is not identified, but it is related to both the dichloromethane used for extraction and the nature of the individual plasma sample.

Improved protein-binding radioassay for plasma cortisol.

1980 ◽  
Vol 26 (2) ◽  
pp. 312-313 ◽  
Author(s):  
H Novacenko ◽  
F Halpern ◽  
E J Sachar
Keyword(s):  
Protein Binding ◽  
Excellent Agreement ◽  
Filter Paper ◽  
Plasma Cortisol ◽  
Rapid Analysis ◽  
Paper Strip ◽  
Total Plasma ◽  
Ethanol Extraction ◽  
Filter Paper Strip ◽  
Competitive Protein Binding

Abstract We describe an improved competitive protein-binding radioassay for total plasma cortisol, which permits rapid analysis of many samples. Plasma, 0.1 mL, is directly applied to a Whatman 3MM filter-paper strip, which is extracted once with dichloromethane. The extract is evaporated and the residue is assayed directly by the micro-method of Murphy (J. Clin. Endocrinol. Metab. 27: 973, 1967). Comparison with the original Murphy method (ethanol extraction) yielded excellent agreement (r = 0.988). One hundred samples, in duplicate, can easily be assayed at a time.

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