VARIATIONS IN PLASMA CONCENTRATIONS OF VASOPRESSIN DURING THE MENSTRUAL CYCLE

1981 ◽  
Vol 89 (2) ◽  
pp. 263-266 ◽  
Author(s):  
MARY L. FORSLING ◽  
M. ÅKERLUND ◽  
P. STRÖMBERG
Keyword(s):  
Menstrual Cycle ◽  
Plasma Concentrations ◽  
Hormonal Changes ◽  
Vasopressin Release ◽  
Healthy Women ◽  
Plasma Vasopressin ◽  
The Mean ◽  
Mean Concentration

Plasma vasopressin concentrations, determined by radioimmunoassay, were followed throughout the menstrual cycle in eight healthy women. The concentrations were found to depend on the day of the menstrual cycle. The mean concentration on day 1 was 0·5±0·08 (s.e.m.) μu./ml, while that on days 16–18 was 1·1±0·16 μu./ml. These values were significantly (P <0·02) different. Vasopressin release in women may thus depend on the hormonal changes during the menstrual cycle.

PROGESTERONE IN THE HUMAN PERIPHERAL BLOOD IN THE PREOVULATORY PERIOD OF THE MENSTRUAL CYCLE

1967 ◽  
Vol 55 (1) ◽  
pp. 91-96 ◽  
Author(s):  
Benno Runnebaum ◽  
Josef Zander
Keyword(s):  
Menstrual Cycle ◽  
Corpus Luteum ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Progesterone Concentration ◽  
Plasma Progesterone ◽  
Progesterone Secretion ◽  
Graafian Follicle ◽  
The Mean ◽  
Mean Concentration

ABSTRACT Progesterone was determined and identified in human peripheral blood during the preovulatory period of the menstrual cycle, by combined isotope derivative and recrystallization analysis. The mean concentration of progesterone in 1.095 ml of plasma obtained 9 days before ovulation was 0.084 μg/100 ml. However, the mean concentration of progesterone in 1.122 ml of plasma obtained 4 days before ovulation was 0.279 μg/100 ml. These data demonstrate a source of progesterone secretion other than the corpus luteum. The higher plasma-progesterone concentration 4 days before ovulation may indicate progesterone secretion of the ripening Graafian follicle of the ovary.

Osmoregulation of thirst and vasopressin during normal menstrual cycle

1988 ◽  
Vol 254 (4) ◽  
pp. R641-R647 ◽  
Author(s):  
T. J. Vokes ◽  
N. M. Weiss ◽  
J. Schreiber ◽  
M. B. Gaskill ◽  
G. L. Robertson
Keyword(s):  
Menstrual Cycle ◽  
Luteal Phase ◽  
Plasma Osmolality ◽  
Free Water ◽  
Urine Osmolality ◽  
Free Water Clearance ◽  
Vasopressin Release ◽  
Plasma Vasopressin ◽  
Normal Menstrual Cycle ◽  
Basal Plasma

Changes in osmoregulation during normal menstrual cycle were examined in 15 healthy women. In 10 women, studied repetitively during two consecutive menstrual cycles, basal plasma osmolality, sodium, and urea decreased by 4 mosmol/kg, 2 meq/l, and 0.5 mM, respectively (all P less than 0.02) from the follicular to luteal phase. Plasma vasopressin, protein, hematocrit, mean arterial pressure, and body weight did not change. In five other women, diluting capacity and osmotic control of thirst and vasopressin release were assessed in follicular, ovulatory, and luteal phases. Responses of thirst and/or plasma vasopressin, urine osmolality, osmolal and free water clearance to water loading, and infusion of hypertonic saline were normal and similar in the three phases. However, the plasma osmolality at which plasma vasopressin and urine osmolality were maximally suppressed as well as calculated osmotic thresholds for thirst and vasopressin release were lower by 5 mosmol/kg in the luteal than in the follicular phase. This lowering of osmotic thresholds for thirst and vasopressin release, which occurs in the luteal phase, is qualitatively similar to that observed in pregnancy and should be taken into account when studying water balance and regulation of vasopressin secretion in healthy cycling women.

Hormonal changes in post-partum blood plasma and milk of buffaloes (Bubalus bubalis)

1983 ◽  
Vol 37 (2) ◽  
pp. 237-246 ◽  
Author(s):  
G. S. Pahwa ◽  
R. S. Pandey
Keyword(s):  
Plasma Concentration ◽  
Blood Plasma ◽  
Post Partum ◽  
Bubalus Bubalis ◽  
Complete Regression ◽  
Hormonal Changes ◽  
Similar Concentration ◽  
The Mean ◽  
A Minor ◽  
Mean Concentration

ABSTRACTPost-partum changes in the concentration of progesterone, oestradiol-17β and prolactin were measured by radioimmunoassay in the blood plasma and milk of 28 buffaloes. The mean concentration of progesterone in the plasma of normal animals was 0·24 ± 0·04 μ.g/1 on the day of calving and decreased linearly during the post-partum period, until the complete regression of the residual corpus luteum of pregnancy occurred, when the plasma concentration was recorded as 0μ05 to 0μ08 μg/l. The concentration at a silent oestrus was 0μ17 ± 0·02 μ.g/1. The milk progesterone profile was parallel to that of plasma but the level was 2 to 4 times higher.The mean concentration of oestradiol-17β in plasma was 41·0 ± 2·34 ng/1 on the day of calving; it declined significantly by day 1 post partum (P < 0·001) and fluctuated between 8 and 16 ng/1 until oestrus, when the concentration was approximately 25 ng/1. The oestradiol-17β concentration in milk was significantly higher than that of plasma on the day of calving. During the first 20 days post partum, a similar concentration of oestradiol-17β was maintained in both fluids but thereafter the concentration in milk was 2 to 3 times that of plasma.The mean concentration of prolactin in plasma was 212·6± 19·08 μg/1 on the day of calving and then declined and fluctuated between 70 and 270 μg/1 during the rest of the period. A minor elevation occurred around oestrus. The prolactin concentration for the first 2 days post partum in milk was significantly higher than that in plasma but during the rest of the period it was similar to that in plasma.The progesterone concentrations were slightly lower in anoestrous animals than in those cycling. The concentration of prolactin was significantly higher in multiparous animals (P < 0·001), whilst the progesterone and oestradiol values were similar in primiparous and multiparous animals.

RADIOIMMUNOASSAY OF OESTRONE AND OESTRADIOL IN HUMAN PLASMA

1972 ◽  
Vol 69 (3) ◽  
pp. 567-582 ◽  
Author(s):  
Yvonne Emment ◽  
William P. Collins ◽  
Ian F. Sommerville
Keyword(s):  
Menstrual Cycle ◽  
Human Plasma ◽  
Simple Method ◽  
Healthy Women ◽  
Theoretical Error ◽  
Dextran Coated Charcoal ◽  
Coefficients Of Variation ◽  
The Mean

ABSTRACT A simple method for the determination of oestrone and oestradiol in human plasma is described and evaluated in terms of theoretical and practical errors. The oestrogens are separated on columns of Sephadex LH 20 and, after equilibration with antiserum, the unbound steroid is removed with dextran-coated charcoal. The mean total random theoretical error is calculated to be 22 %; the coefficients of variation of replicate analyses on pooled female plasma were from 13–18 % for both steroids; in male plasma the values were 17 % for oestradiol and 27 % for oestrone. The concentrations of oestradiol, expressed in pg/ml ± sd) in samples collected from healthy women during the menstrual cycle were 69 ± 56 (days 1–10); 126 ± 66 (days 11–20) and 99 ± 54 (days 21–30). Corresponding values for oestrone were 119 ± 46, 156 ± 41 and 156 ± 27.

Evidence of altered dopaminergic modulation of Prl, LH and TSH secretion in patients with normoprolactinaemic amenorrhoea

1984 ◽  
Vol 106 (1) ◽  
pp. 8-14 ◽  
Author(s):  
Claus Hagen ◽  
Karsten Petersen ◽  
Henning Djursing ◽  
Anders Nyboe Andersen
Keyword(s):  
Plasma Concentrations ◽  
Ideal Body Weight ◽  
Control Group ◽  
Hormonal Changes ◽  
Tsh Secretion ◽  
Basal Plasma ◽  
Hormone Responses ◽  
The Mean ◽  
Normal Women ◽  
Plasma Prl

Abstract. Basal plasma concentrations of Prl, LH, FSH, GH, TSH, T3, T4, resin T3 uptake (RT3U), and oestradiol as well as hormone responses to iv metoclopramide (MTC) were investigated in 16 consecutive patients with normoprolactinaemic, normogonadotrophic amenorrhoea. The control group consisted of 17 normal menstruating women between day 3 and 6 of the menstrual cycle. The mean age of the amenorrhoeic patients was 24.0 years (range 19 to 34) and the mean duration of amenorrhoea was 31 months (range 12 to 60). Amenorrhoeic patients had significantly (P < 0.05) lower basal levels of LH, oestradiol and RT3U, whereas other hormone levels were similar in the two groups. Plasma Prl and TSH concentrations rose significantly (P < 0.05) after the administration of MTC in the two groups. A significant positive correlation (r = 0.69 P < 0.01) was found between the TSH response to MTC and basal TSH levels in controls, but not in amenorrhoeic patients. Plasma LH levels increased significantly (P < 0.05) in amenorrhoeic patients, but not in controls. The Prl and TSH responses to MTC were significantly (P < 0.001) lower in amenorrhoeic patients than in normal women. In amenorrhoeic patients none of the hormonal parameters correlated significantly (P > 0.05) with the percentage of ideal body weight. It is concluded that the hormonal changes in amenorrhoeic patients may in part be caused by a raised dopaminergic acvitity leading to a depression of central ovulatory mechanisms.

Effect of ovariectomy and treatment with ovarian steroids on vasopressin release and fluid balance in the rat

1990 ◽  
Vol 124 (2) ◽  
pp. 277-284 ◽  
Author(s):  
K. Peysner ◽  
M. L. Forsling
Keyword(s):  
Water Intake ◽  
Fluid Balance ◽  
Plasma Concentrations ◽  
Plasma Osmolality ◽  
Control Group ◽  
Oestrogen Treatment ◽  
Vasopressin Release ◽  
Plasma Vasopressin ◽  
Progesterone Treatment ◽  
Food And Water Intake

ABSTRACT Plasma vasopressin concentrations have previously been shown to vary during the oestrous cycle of the rat, being highest on the morning of pro-oestrus and lowest on dioestrus day 1. To determine the effect of gonadal steroids on vasopressin secretion and fluid balance, mature rats were ovariectomized and given oestrogen, progesterone or vehicle alone s.c. for periods of up to 16 days. Plasma vasopressin concentrations fell after ovariectomy and this was reflected in an increase in 24-h urine volume. The normal increase in plasma vasopressin concentrations seen over daylight hours was also suppressed. The change in vasopressin concentrations observed on steroid treatment depended upon both the dose and the duration. High doses of oestrogen were associated with a fall in plasma vasopressin, probably as a result of fluid retention. Thus, of an initial group of rats given silicone elastomer implants containing 50, 500 or 1000 μg oestradiol in oil, plasma vasopressin concentrations were reduced after 7 days treatment with 1000 μg oestradiol implants in association with reduced plasma sodium concentrations. Daily s.c. injections of 100 μg oestradiol benzoate/100 g body weight produced an immediate small increase in plasma vasopressin concentrations, but by 14 days the plasma concentrations of 0·7 ± 0·16 pmol/l (mean ± s.e.m.) had fallen significantly and were less than those in the vehicle-treated group (1·2± 0·26 pmol/l). However, after treatment for 14 days with implants containing only 50 μg oestradiol, plasma vasopressin concentations were higher compared with the group receiving vehicle alone, despite the fact that the plasma osmolality was lower in the latter group, suggesting a long term resetting of the osmoreceptors. Progesterone treatment with two implants containing 17·5 mg progesterone in oil was associated with an initial suppression of plasma vasopressin concentrations, but 16 days after the implant the plasma concentrations were higher than in the control group. Neither oestrogen nor progesterone restored the vasopressin concentrations to those seen in the intact animal. Oestrogen treatment resulted in a reduction in food and water intake, whereas progesterone treatment produced an initial increase in food and water intake, and a fall in plasma osmolality which could account for the reduced plasma vasopressin. This was followed by an increase in urine flow over days 6 to 15. Thus ovariectomy had a marked effect on circulating vasopressin concentrations, probably as a result of complex changes since administration of either oestrogen or progesterone in doses giving normal circulating concentrations had little effect. Journal of Endocrinology (1990) 124, 277–284

Serotonin Concentrations in Plasma and Variations During the Menstrual Cycle

1992 ◽  
Vol 38 (10) ◽  
pp. 2087-2089 ◽  
Author(s):  
I Hindberg ◽  
O Naesh
Keyword(s):  
Menstrual Cycle ◽  
Healthy Volunteers ◽  
Plasma Concentrations ◽  
Reference Interval ◽  
Serotonin Concentration ◽  
Very Old ◽  
Menstrual Cycles ◽  
Platelet Poor Plasma ◽  
Healthy Women

Abstract We used a specific and sensitive radioenzymatic method to establish a reference interval for the concentration of serotonin in platelet-poor plasma in 98 healthy volunteers (49 men, 49 women). The interval was 0-11 nmol/L with a median of 2.8 nmol/L. No difference in concentration in relation to sex or age was observed. In a group of eight very old volunteers (ages 86-92 years), however, concentrations were increased. In addition, we monitored the plasma concentrations of serotonin in 20 healthy women (ages 26-45 years) through two menstrual cycles. Periovulatory and premenstrual concentrations were greater than the serotonin concentration at the start of menstruation.

scholarly journals Variation in the Hearing Threshold in Women during the Menstrual Cycle

2017 ◽  
Vol 21 (04) ◽  
pp. 323-328 ◽  
Author(s):  
Dayse Souza ◽  
Brunna Luckwu ◽  
Wagner Andrade ◽  
Luciane Pessoa ◽  
João Nascimento ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Hearing Threshold ◽  
Hormonal Contraceptives ◽  
Control Group ◽  
Hormonal Changes ◽  
High Frequencies ◽  
Hearing Thresholds ◽  
Hormonal Fluctuations ◽  
The Mean ◽  
Late Follicular Phase

Introduction The hormonal changes that occur during the menstrual cycle and their relationship with hearing problems have been studied. However, they have not been well explained. Objective The objective of our study is to investigate the variation in hearing thresholds in women during the menstrual cycle. Method We conducted a cohort and longitudinal study. It was composed of 30 volunteers, aged 18–39 years old, of which 20 were women during the phases of the menstrual cycle and 10 were men (control group) who underwent audiometry and impedance exams, to correlate the possible audiological changes in each phase of the menstrual cycle. Results There were significant changes in hearing thresholds observed during the menstrual cycle phases in the group of women who used hormonal contraceptives and the group who did not use such contraceptives. Improved hearing thresholds were observed in the late follicular phase in the group who did not use hormonal contraceptives and the hearing thresholds at high frequencies were better. Throughout the menstrual cycle phases, the mean variation was 3.6 db HL between weeks in the group who used hormonal contraceptives and 4.09 db HL in the group who did not use them. Conclusions The present study found that there may be a relationship between hearing changes and hormonal fluctuations during the menstrual cycle based on changes in the hearing thresholds of women. In addition, this study suggests that estrogen has an otoprotective effect on hearing, since the best hearing thresholds were found when estrogen was at its maximum peak.

Time-resolved immunofluorometric assay of 17 beta-hydroxysteroid dehydrogenase in plasma

1991 ◽  
Vol 37 (8) ◽  
pp. 1412-1415 ◽  
Author(s):  
O Mäentausta ◽  
M Menjivar ◽  
R Vihko
Keyword(s):  
Polyclonal Antibodies ◽  
Endometrial Adenocarcinoma ◽  
Plasma Concentrations ◽  
Hydroxysteroid Dehydrogenase ◽  
Minimum Detectable Concentration ◽  
Antibody Molecule ◽  
Time Resolved ◽  
Detectable Concentration ◽  
The Mean ◽  
Mean Concentration

Abstract We describe a time-resolved immunofluorometric assay (TR-IFMA) for human 17 beta-hydroxysteroid dehydrogenase (17HSD) in which antibody-coated microtiter strip wells and europium chelate-labeled polyclonal antibodies are used. In preparing the label, a polyclonal antibody is affinity-purified and derivatized with diethylenetriamine-pentaacetic acid. With this derivative, five to eight europium ions can be combined with one antibody molecule without decreasing the antibody's immunoreactivity. The minimum detectable concentration of 17HSD is 0.13 microgram/L; the intra- and interassay CVs are less than 8% and less than 15%, respectively, for concentrations between 0.3 and 100 micrograms/L. There is no difference between the concentrations of 17HSD in plasma specimens taken during the proliferative and luteal phases of the menstrual cycle, the measured mean concentration being 0.22 microgram/L. We found no correlation between plasma 17HSD and progesterone concentrations. The plasma concentrations of 17HSD increase during pregnancy, the mean concentrations being 1.5, 4.4, and 12.5 micrograms/L, during the first, second, and third trimesters of pregnancy, respectively. In the specimens from 18 men, the mean concentration was 0.18 microgram/L. In six plasma specimens from patients with endometrial adenocarcinoma, the mean concentration was 0.20 micrograms/L. Pre-analytical aspects are important in the assay of 17HSD because of the lability of the enzyme protein. Preferably, blood should be sampled into EDTA-containing tubes, plasma should be separated within 15 min, and glycerol must be added without delay to a final volume of 200 mL/L.

scholarly journals Plasma and ovarian oestradiol and the variability in the LH surge induced in ewes by the ram effect

Reproduction ◽  
2015 ◽  
Vol 149 (5) ◽  
pp. 511-521 ◽  
Author(s):  
Claude Fabre-Nys ◽  
Audrey Chanvallon ◽  
Nathalie Debus ◽  
Dominique François ◽  
Frédéric Bouvier ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Plasma Concentrations ◽  
Feedback Signal ◽  
Sexually Active ◽  
Lh Surge ◽  
Before And After ◽  
Ram Effect ◽  
The Mean ◽  
Mean Concentration

The proportion of anoestrous ewes ovulating after exposure to a sexually active ram is variable mainly due to whether an LH surge is induced. The aim of this study was to determine the role of oestradiol (E2) in the ram-induced LH surge. In one study, we measured the plasma concentrations of E2 in ewes of different breeds before and after the ‘ram effect’ and related these patterns to the presence and latency of the LH surge, while another compared ovarian responses with the ‘ram effect’ following exposure to rams for 2 or 12 h. In all ewes, the concentration of E2 increased 2–4 h after rams were introduced and remained elevated for 14.5±0.86 h. The quantity of E2 secreted before the LH surge varied among breeds as did the mean concentration of E2. The granulosa cells of IF ewes collected after 12 h exposure to rams secreted more E2 and progesterone and had higher levels of StAR than the 2 h group but in MV ewes there was no differences between these groups for any of these parameters. These results demonstrate that the LH surge induced by the rams is a result of increased E2 secretion associated with increased levels of STAR in granulosa cells and that these responses varied among breeds. The results suggest that the variable occurrence of a LH surge and ovulation may be the result of variable ovarian responses to the ‘ram effect’ and insensitivity of the hypothalamus to the E2-positive feedback signal.Free French abstract: A French translation of this abstract is freely available at http://www.reproduction-online.org/content/149/5/511/suppl/DC1.

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